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Dgkb (NM_019304) Rat Tagged ORF Clone
CAT＃: RR200972
- TrueORF®
Dgkb (Myc-DDK-tagged ORF) - Rat diacylglycerol kinase, beta (Dgkb), (10 ug)
"NM_019304" in other vectors (3)
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CNY 10,260.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Dagk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200972 representing NM_019304
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAAACCAGGAAAAATGGGCCCATCTCAGCCCCTCAGAATTTTCCCAACTTCAGAAATATGCTGAGT ATTCTACAAAGAAATTAAAGGATGTTCTTGAAGAATTCCATGGTAATGGTGTGCTTGCAAAGTATAATCC TGAAGGGAAACAAGATATTCTTAACCAAACCATAGATTTTGAAGGATTCAAGCTGTTCATGAAGACCTTC CTGGAAGCTGAGCTCCCTGACGATTTCACTGCCCACCTTTTCATGTCATTCAGCAACAAATTCCCCCATT CCAGCCCCAACGTAAAGAGCAAGCCAGCTCTGCTTTCAGGTGGTTTGAGGATGAATAAGGGTGCCATCAC CCCTCCACGTTCTTCTCCAGCAAATACGTGCTTCCCGGAAGTGATCCACCTCAAGGATATTGTCTGTTAC CTGTCTCTTCTGGAAAGAGGAAGACCTGAGGACAAGCTTGAGTTTATGTTTCGCCTGTATGACACCGATG GGAATGGCTTCCTGGACAGCTCGGAATTAGAAAATATCATCGGTCAGATGATGCATGTTGCCGAGTACCT GGAATGGGATGTTACTGAACTTAATCCGATCCTTCATGAAATGATGGAAGAAATCGACTATGACCGTGAC GGCACTGTGTCTCTGGAAGAGTGGATTCAAGGAGGAATGACGACTATCCCACTTCTAGTCTTGCTGGGCT TGGAAAACAATGTGAAGGATGACGGACAGCATGTGTGGCGACTCAAGCACTTTAACAAGCCCGCCTACTG CAATCTCTGCCTGAACATGCTGATTGGCGTGGGGAAGCAGGGCCTCTGCTGCTCCTTCTGCAAGTACACC GTCCATGAGCGCTGTGCCAGAGCCCCGCCCTCCTGCATCAAGACCTATGTGAAGTCCAAGAAGAACACTG ACGTCATGCACCATTATTGGGTCGAAGGCAATTGCCCAACCAAGTGTGATAAGTGCCACAAGACAGTGAA ATGTTACCAGGGCCTGACGGGGCTGCACTGTGTTTGGTGTCAGACCACGCTGCACAATAAATGTGCTTCT CATCTGAAGCCTGAATGTGACTGTGGACCCTTGAAGGACCATATTTTGCCACCCACCACAATCTGTCCAG TTGTATTGACTATGCCCACTGCCGGAACTTCAGTTCCTGAGGAAAGACAGTCAACAGCTAAAAAGGAAAA GGGCAGCTCCCAGCAGCCAAATAAAGTGACAGACAAGAACAAAATGCAAAGAGCTAACTCTGTCACTATG GATGGACAAGGTTTGCAGATCACTCCTATTCCTGGCACACATCCTCTTCTAGTTTTTGTCAACCCTAAAA GTGGTGGGAAGCAAGGAGAACGAATTTACAGAAAATTCCAGTATCTTCTAAATCCTCGTCAGGTTTACAG TCTTTCTGGAAATGGACCAATGCCAGGGTTACATTTTTTTCGCGATGTCCCTGACTTCAGAGTGTTAGCA TGCGGTGGAGATGGGACTGTGGGCTGGATTTTGGACTGCATAGAAAAGGCCAATGTAGTCAAGCACCCTC CAGTTGCTATCCTGCCTCTTGGGACGGGCAATGACCTAGCAAGATGCCTGCGGTGGGGTGGAGGTTATGA AGGTGAAAATCTTATGAAGATCCTAAAAGACATTGAAAGCAGCACAGAAATCATGTTGGACAGGTGGAAG TTTGAAGTCACACCCAACGACAAAGACGAGAAAGGAGACCCTGTGCCTTACAGCATCATCAACAACTACT TTTCCATTGGCGTGGATGCTTCCATTGCACACAGATTCCACATCATGAGAGAAAAGCACCCGGAGAAATT CAACAGCCGAATGAAGAACAAATTTTGGTACTTTGAGTTTGGCACATCAGAAACCTTCTCAGCCACCTGC AAGAAACTGCATGAATCTGTAGAAATAGAATGTGATGGAGTACAGATAGATTTGATAAACATCTCTCTGC AAGGAATTGCAATTTTGAATATACCAAGCATGCATGGAGGATCTAATCTTTGGGGAGAATCTAAAAAGAA AAGAAGTCATCGACGGATAGAGAAAAAAGGCTCTGACAAAAGACCCACTCTCACAGATGCAAAAGAACTG AAGTTTGCAAGTCAAGATCTGAGTGACCAGCTACTGGAGGTTGTTGGCTTGGAAGGAGCAATGGAAATGG GCCAAATATACACGGGGCTGAAAAGTGCTGGCCGGCGGCTGGCTCAGTGCTCCTCTGTGGTTATCAGGAC AAGCAAGTCACTGCCAATGCAGATTGATGGAGAGCCATGGATGCAGACCCCATGCACAATAAAAATTACA CACAAGAACCAGGCCCCAATGCTGATGGGACCTCCACCTAAAACTGGATTATTCTGCTCCCTCATCAAAA GAACAAGAAACCGAAGCAAGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200972 representing NM_019304
Red=Cloning site Green=Tags(s) MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGKQDILNQTIDFEGFKLFMKTF LEAELPDDFTAHLFMSFSNKFPHSSPNVKSKPALLSGGLRMNKGAITPPRSSPANTCFPEVIHLKDIVCY LSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRD GTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYT VHERCARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCAS HLKPECDCGPLKDHILPPTTICPVVLTMPTAGTSVPEERQSTAKKEKGSSQQPNKVTDKNKMQRANSVTM DGQGLQITPIPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLA CGGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWK FEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATC KKLHESVEIECDGVQIDLINISLQGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKEL KFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKIT HKNQAPMLMGPPPKTGLFCSLIKRTRNRSKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019304 |
| ORF Size | 2403 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019304.1, NP_062177.1 |
| RefSeq Size | 6211 bp |
| RefSeq ORF | 2406 bp |
| Locus ID | 54248 |
| UniProt ID | P49621 |
| MW | 90.3 kDa |
| Gene Summary | beta isotype of the diacylglycerol kinase family, regulates intracellular concentrations of the second messenger diacylglycerol (DAG) [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200972 | Dgkb (untagged ORF) - Rat diacylglycerol kinase, beta (Dgkb), (10 ug) |
CNY 10,260.00 |
|
| RR200972L3 | Lenti ORF clone of Dgkb (Myc-DDK-tagged ORF) - Rat diacylglycerol kinase, beta (Dgkb), (10 ug) |
CNY 12,070.00 |
|
| RR200972L4 | Lenti ORF clone of Dgkb (mGFP-tagged ORF) - Rat diacylglycerol kinase, beta (Dgkb), (10 ug) |
CNY 13,300.00 |
