Adgrb2 (NM_001107914) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Bai2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200962 representing NM_001107914 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCCAGCCTGTCCCCTCTTACTGTCTGTGATTCTGTCCCTGCGCCTGGCCACGGCCTTCGACCCTG CCCCCAGTGCCTGCTCTGCCCTGGCCTCGGGCGTGCTCTACGGGGCCTTCTCACTGCAGGACCTCTTTCC CACCATCGCCTCGGGCTGCTCCTGGACCCTGGAGAACCCAGACCCCACCAAGTACTCGCTCTACCTGCGC TTCAACCGACAGGAGCAGGTGTGCACACACTTTGCCCCGCGCCTGCTGCCCCTGGACCACTACCTGGTCA ACTTTACGTGTCTGCGGCCTGGTCCGGAGGAAGCCACAGCCCGGGCGGAATCAGAAGTGGGGCGGCCAGA GGAGGAAGCGGAGGCCGAGGCAGCAGCCACGGGCTTGGAGCTGTGTGGTGGCTCAGGCCCTTTCACCTTT CTGCACTTCGACAAGAACTTCGTGCAGCTGTGCCTGTCGGCTGAGCCCTCGGAGGCCCCACGTCTGCTAG CGCCTGCTGCCCTGGCCTTCCGCTTTGTGGAGGTTTTGCTTATCAACAACAACAACTCCAGCCAGTTCAC CTGCGGCGTGCTCTGCCGCTGGAGTGAGGAGTGTGGTCGGGCTGCAGGCAGGGCTTGTGGCTTTGCTCAG CCAGGGTGTAGCTGCCCTGGGGAGGCTGGGGCCAGCCCTGCCACCACCACACCTCCGGGGCCTCCAGCTG CCCACACCCTGTCCAATGCCTTGGTGCCTGGGGGCCCAGCCCCTCCTGCTGAGGCCGACTTGCACTCGGG GAGCAGCAATGACCTGTTCACCACCGAGATGAGATACGGTGAGGAGCCGGAAGAGGAACCGAAGGTGAAA ACCCAGTGGCCAAGGTCTGCAGATGAGCCTGGGCTATACATGGCGCAGACAGGCGACCCAGCAGCTGAGG AGTGGTCCCCGTGGAGCGTGTGTTCCCTGACGTGTGGGCAGGGTCTGCAGGTGCGGACCCGCTCCTGCGT GTCCTCCCCCTATGGGACCCTGTGCAGCGGGCCCCTTCGGGAGACCCGGCCTTGCAACAATTCAGCCACC TGCCCAGTGCACGGCGTGTGGGAGGAGTGGGGGTCCTGGAGCCTGTGCTCCCGCAGCTGCGGGCGGGGGT CCCGGAGTCGGATGCGGACCTGCGTGCCCCCCCAGCACGGCGGCAAGGCCTGCGAGGGTCCCGAGCTGCA GACTAAACTCTGCAGTATGGCCGCCTGCCCGGTGGAAGGCCAGTGGCTAGAATGGGGTCCCTGGGGCCCA TGCTCCTCATCTTGTGCCAATGGGACCCAGCAGCGCAGCCGGAAGTGCAGTGTGGCCGGTCCAGCCTGGG CCACGTGCGCAGGTGCCCTCACCGATACCCGTGAGTGCAGCAACTTCGACTGTCCAGCCACTGATGGCAA GTGGGGGCCATGGAACGCATGGAGCCTGTGCTCCAAGACGTGTGACACGGGCTGGCAACGCCGCTTCCGC ATGTGCCAGGCTTCCGGCACGCAGGGCTACCCTTGCGAGGGCACGGGAGAGGAGGTGAAACCCTGCAGTG AGAAGAGGTGTCCAGCCTTCCATGAGATGTGCAGAGACGAGTACGTCATGCTCATGACGTGGAAGAGGGC AGCGGCTGGCGAGATCATTTACAACAAATGTCCCCCTAATGCCTCGGGTTCTGCTAGCCGCCGCTGTCTC CTCAGTGCCCAGGGCGTGGCATACTGGGGACTGCCCAGTTTCGCTCGTTGTATATCTCATGAATACCGAT ACCTGTACCTGTCACTTCGGGAGCACCTGGCTAAGGGCCAGCGCATGCTGGCAGGTGAGGGCATGTCGCA GGTGGTGCGGAGCCTACAGGAGCTCCTGGCTCGGCGTACTTACTACAGCGGGGACCTGCTCTTCTCTGTG GACATTCTAAGGAACGTCACTGACACCTTCAAGAGGGCCACCTATGTCCCTTCCGCTGATGACGTGCAGC GTTTCTTCCAGGTGGTGAGCTTTATGGTGGACTCAGAAAACAAGGATAAGTGGGACGACGCTCAGCAGGT GTCGCCTGGCTCTGTGCACTTGCTGCGTGTTGTGGAAGATTTCATTCACCTCGTGGGCGACGCTCTCAAG GCCTTCCAGAGCTCTCTGATTGTCACGGACAACCTGGTGATCAGCATTCAGAGAGAGCCTATCTCAGCGG TGTCCAGTGACATCACATTTCCCATGCGGGGCCGCAGGGGCATGAAGGACTGGGTACGACATTCAGAGGA TCGCCTCTTCCTACCCAAGGAGGTGCTCAGCCTGTCCTCCCCAGGGAAGCCAGCCACACCTGGGGCAGCC ACAGCGGGCAGCCCGGGCAGGGGGAGGGGCCCAGGAACGGTGCCCCCTGGCCCAGGCCCCGCCCACCAGC GCCTTCTCCCAGCTGACCCCGAAGAGTCCTCCTCCTACTTTGTGATCGGTGCTGTGCTCTACCGCACCCT TGGCCTCATCCTGCCGCCCCCCAGGCCTCCACTTGCTGTCACCTCCCGGGTGATGACAGTGACTGTGCGT CCCCCCACCCAGCCTCCAGCGGAGCCCCTCATTACAGTAGAGCTCTCGTACATCATCAATGGCACCACTG ATCCCCACTGTGCCAGCTGGGACTACTCGAGAGCAGATGCCAGCTTGGGGGACTGGAACACTGAGAGCTG CCAGACCTTGGAGACCCAAGCAGCTCACACCCGCTGCCAGTGCCAGCACCTGTCCACCTTTGCCGTCCTG GCCCAGCCACCCAAGGACCTGACTCTGGAGCTGGCGGGTGCTCCCTCTGTCCCCCTGGTGATCGGCTGTG CGGTGTCCTGCATGGCCCTGCTCACGTTGCTGGCCATCTATGCAGCCTTCTGGAGGTTTATAAAGTCAGA ACGCTCCATCATCTTGCTGAACTTCTGCCTGTCCATCCTGGCTTCCAACATCCTGATCCTCGTGGGCCAG TCCCGGGTGCTGAGCAAGGGCGTATGCACCATGACAGCTGCCTTCCTACACTTCTTCTTTCTGTCCTCCT TTTGCTGGGTGCTTACAGAGGCCTGGCAATCCTATCTGGCTGTCATCGGGCGGATGCGCACCCGCCTGGT TCGCAAGCGCTTCCTCTGCCTGGGCTGGGGTCTGCCTGCCCTGGTGGTGGCTGTGTCTGTTGGCTTTACT CGCACCAAAGGATACGGCACATCCAGCTACTGCTGGCTGTCCCTAGAGGGTGGCCTGCTCTACGCCTTTG TGGGTCCAGCAGCAGTCATCGTCCTGGTGAACATGCTCATCGGAATCATCGTCTTCAACAAGCTCATGGC TCGAGATGGCGTCTCAGACAAGTCTAAGAAGCAGAGGGCCGGGTCGGAGCGGTGCCCCTGGGCCAGCCTG CTCCTCCCCTGCTCAGCGTGTGGAGCGGTCCCCAGCCCCCTGCTCAGCTCAGCCTCGGCCAGGAATGCCA TGGCTTCCCTCTGGAGTTCCTGCGTGGTACTGCCTCTCCTGGCGCTTACCTGGATGTCCGCTGTCCTGGC CATGACAGATCGCCGCTCCGTCCTCTTCCAGGCGCTCTTTGCTGTTTTCAACTCTGCACAAGGCTTTGTC ATCACCGCCGTGCACTGCTTCCTGCGCCGAGAGGTCCAGGATGTGGTAAAGTGCCAGATGGGTGTGTGTC GGACTGATGAGAGTGAAGACTCCCCAGACTCGTGCAAGAACGGACAGCTGCAGATCCTGTCAGACTTTGA AAAGGACGTGGATCTGGCTTGTCAGACAGTTCTGTTCAAGGAGGTCAACACTTGCAACCCGTCCACCATC ACCGGCACTTTGTCCCGCCTGTCTCTGGATGAGGATGAGGAGCCCAAGTCCTGTCTCGTGGGTCCTGAGG GTGGCCTCAGCTTCTCACCACTGCCTGGGAACATCCTAGTACCCATGGCAGCCTCGCCAGGTCTGGGGGA GCCACCACCACCACAGGAGACCAACCCTGTGTACATGTGTGGGGAGGGTGGCCTGCGGCAATTGGACCTT ACATGGCTACGGCCAAGTGAACCAGGCTCTGAGGGGGACTACATGGTGCTGCCCCGGCGGACTTTGAGCC TGCAGCCTGGCGGTGGGGGTACAGGGGGTGAGGAGGCACCAAGGGCCCGGCCTGAGGGGACCCCCCGGCG GGCTGCCAAGACAGTGGCCCACACTGAAGGCTACCCCAGCTTCTTGTCTGTGGAGCACTCGGGTCTAGGG CTGGGCCCTGCCTATGGGTCTCTCCAGAACCCATATGGGATGACCTTCCAACCACCACCACCAACACCCA GCGCCCGCCAAGTACCAGAGCCAGGAGAACGTAGCCGGACCATGCCCCGTACAGTGCCTGGTTCCACCAT GAAGCTGGGCTCCCTTGAGCGAAAGAAGCTTCGGTATTCTGACTTGGACTTTGAGAAGGTGATGCATACC CGGAAACGGCACTCGGAACTCTACCACGAACTCAACCAGAAGTTCCACACTTTCGACCGCTACCGTAGCC AGTCCTCAGCCAAGGAGAAACCCAGCCCCCCTGGGGGACGCCCTGGCTTGTCCCAGCACAGGAGGCATCA AAGCTGGAGCACTTTCAAATCTATGACACTGGGCTCACTGCCCCCCAAACCTCGAGAACGGCTGGCTCTG CACCGGACAGCAGCCTGGGAGCCCACAGAACCGCCAGACGGCGACTTCCAGACAGAGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200962 representing NM_001107914 Red=Cloning site Green=Tags(s) MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLR FNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEAEAEAAATGLELCGGSGPFTF LHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQ PGCSCPGEAGASPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVK TQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSAT CPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGP CSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNFDCPATDGKWGPWNAWSLCSKTCDTGWQRRFR MCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCL LSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSV DILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALK AFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAA TAGSPGRGRGPGTVPPGPGPAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVR PPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASLGDWNTESCQTLETQAAHTRCQCQHLSTFAVL AQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQ SRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFT RTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASL LLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFV ITAVHCFLRREVQDVVKCQMGVCRTDESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTI TGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDL TWLRPSEPGSEGDYMVLPRRTLSLQPGGGGTGGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLG LGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHT RKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLAL HRTAAWEPTEPPDGDFQTEV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107914
ORF Size	4680 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107914.1, NP_001101384.1
RefSeq Size	5001 bp
RefSeq ORF	4683 bp
Locus ID	313058
MW	169.7 kDa

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