Siglec1 (NM_001107777) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Sn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200553 representing NM_001107777 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGCCCTGCTCTCCTTGCTCCTGCTGGCCTCGGTCTTCACACCAGGCCAGACCACGTGGGGTGTCT CCAGTCCCAAGGATGTGCAGGGCTTGACTGGATCTTGCCTGCTCATTCCCTGCATCTTCAGCTTCCCTGC TGATGTATCAGTGCCTAATGGCATCACAGCCATCTGGTACTATGACTACTCGGGCAAACGGCAGGTGGTA ATCCACTCAGGGGACCCCAAGCTGGTGGATGAGCGCTTCAGGGGCAGAGCTGAACTGACAGGGAACATGG ATCACAAGGTGTGCAACCTGTTGCTCAAAGACCTGAAGCTTGAAGACTCAGGCACCTACAACTTCCGCTT TGAGATCAGTGATGGCAACCGCTGGTCTGATGTCAGAGGCACCACAGTCACTGTGACAAAGGATCCCAGT CCCCCCACTATTACCATCCCACATGAGCTGCGTGAAGGTGTGGAGGTGAACGTCAACTGTTCCACACCCT ACCTGTGCCTGCAGGAGAAGCAGGTCAGCCTGCATTGGCAAGGCCAGGACCCCACCCGCTCCGTCATCTC CAACTTCCAGAGCCTCGAGCCCACTGGCGTCTACCACCAGACGACCCTACATATGGCCCCATCCTGGCAG GACCACAGACGGACCCTGCGCTGCCAGCTGTCATTGGCCACGCACAGTAGTCAGAAAGAGGTTTATCTAC AAGTGCAGCATGCCCCCAAAGGTGTGGAGATCCTCCTTAGCTCCTCAGGAAGGAACATCCTTCCAGGGAA CCTGGTCACGCTCACCTGCCGGGTGAATAGCAGCTATCCTGCCGTCAGTTCTGTGGATTGGGTCAAGGAT GGAGTGAACCTCACAGCCAAGGGGCATGTGCTCCAGCTATCCTCAGCAACCTGGAATGATTCTGGGGTCT ACACCTGTCAAGCAACAAATAATGTGGGCTCTCTGGCATCACCCCCACTCAGCCTCCACGTTTTTATGGC TGAAGTCAAGATAAACCCAGCAGGGCCCATCTTGGAAAATGAGACAGTGACACTCCTCTGTAGCACCCCC AAGGAGGCACCCCAGGAGCTCCGCTACAGCTGGTACAAGAACAACATTCTTCTGGAAGACGCCCATGCCC CAACCTTGCACCTGCCTGCAGTCACCAGGGCTGATACTGGCTTCTATTTCTGTGAGGTGCAGAACACCCA GGGCAGTGAGCGCTCCGGTCCAGTGAGTGTGGTGGTCAGATATCCACCTCTTACTCCAGACCTGACCACC TTCTTGGAGACACAGGCTGGACTTGTGGGCATCTTCCATTGCTCTGTGATCAGCGAGCCCCTGGCTACTG TGGTACTGTCACACGGAGGTCTCACATTGGCCTCCAGCTCTGGAGAAAATGACTTCAACCCCCGATTCAG CATTTCCTCTGCCCCCAACTCCGTGCGCCTAGAAATCCGAGACTTGCAGCCAGCGGACAGCGGGGAGTAC ACATGCTCAGCCACCAACTCCCTTGGAAAATCAATGTCCAGCCTAGACTTCCATGCAAATGATCCCCCAA GAAAGCTCACATTCACTGCCAGGCTGGATTTGGATACCTCTGGAGACGGGGATGGACGGCGTGGCATCCT CTTGTGCCAAGTAGACAGTGATCCCCCAGCCCAGCTACAGCTTCTCCACAAAGGTCATGTTGTGGCCACT TCTCTGCCATCAATGTGTGGGAGCTGCTCTCGGCGCATGAAAGTCAGCAGAGCCTCCAACTCACTGCAGG TGGAGATCCAGAAGCCGGTACTAGAGGATGAGGGCATGTACCTGTGTGAGGCTAGCAACACACTGGGCAA CTCCTCTGTCTCAGCCTCTTTCAATGCTAAGGCTACTGTCGTGGTCATCACACCGTCAGATACACTGCTT GAAGGCACAGAAGCCAGCCTAACTTGTAACGTGACCCAGGAGGTTGCCGTCAGCCCTGCCAACTTTTCTT GGTTCCGGAATGGAGTGCTGTGGACCCAGGGACCACTGGAGACTGTGAGGCTGCAGCCTCTGGCCAGGAC TGATGCTGCCGTCTATGCCTGCCGCCTCCTCACTGACGATGGGGCCCAGCTCTCGGCTCCTGTGGTCCTA AGTGTGCAGTATGCCCCAGACCCTCCAAAGCTGTCAGCCCTCCTGGATGTGGGTCAGGGCCACATGGCTG TGTTCATCTGCACTGTGGACAGCTATCCCCTGGCTCACCTGTCTCTATTCCGTGGGGAGCGCCTCCTGGC CACCAGCTTGGAACCCCAGCGTCTATCCCATGGTAGAATCCAGGCCAAGGCCACAGCCAACTCCCTGCAG CTAGAGGTCCGGGAACTAGGCCTTGAAGACTCTGGAAACTACCACTGTGAAGCCACAAATGTTCTTGGAT CAGCCAACAGTTCACTCTTCTTCCAGGTCAGAGGAGCCTGGGTCCAGGTTTCGCCATCACCTGAGCTCCG AGAGGGCCAGGCTGTGGTCCTGAGCTGCCAGGTGCCCACAGGGGTGCCTGAGGGAACCTCATACCGCTGG TTTCAGGATGGCCGCCCCCTCCAGGAGTCAACCTCATCTATATTGCGCATTGCAGCCATAAGTCTGAGGC AAGCTGGTGCCTACCATTGCCAAGCTCAGGCTCCAGACACAGCTGCAGCCAGCCTGGCTGCCCCTGTCAG CCTCCATGTGTCCTATACCCCACGTCACGTGACACTCAGCGCCCTGCTGAGCACGGGCCCTGGGCGATAC GGCCACCTGGTGTGCAGTGCACAAAGTGACCCTCCAGCTCTGCTGCGACTGTTCCACCAGAATCGCCTCG TGGCCTCTACCCTACAAGGCCCGGAGGAATTGGCAAGCAGTAATCCCCGGCTGCATGTGGCTGTGAGCTC CAATGAGTTGCGCCTGGAGATCCAATTCACAGAGCTGGAGGATGGTGGGACCTACACATGTGAAGCCACC AACACACTGGGCCAGGCATCTGCTACAGCTGACTTCGATGCCCAGGCTGTGCGTGTGGCCGTGTGGCCCA ATGCCACTGTGCAAGAGGGGCAGCAGGTGAACCTCAGCTGCTTGGCGTGGAGCACCCACCAGGACTCACT CAGCTACACATGGTACAAGGGCGGCCAACAACTGCTTGGTGTCAGATCCATCTCCCTGCCCAGCGTTACA GTTTCGGATGCTACCTCCTACCGCTGCGGTGTGGGGCTCCCTGGCCACACACCCCATCTCTCCAGACCTG TGACCCTGGATGTCCTCTACGCTCCCCGAAGCCTGCAGCTGACCTACCTCCTGGAGAGCCAGGGCCGGCG GCTGGCCCTGGTACTGTGTACTGTGGATAGTCGCCCACCTGCCCAGCTAACTCTCAGCCACGGTGGCCAG CTCCTAGCCTCCTCCACTGAAGCTTCCGTCCCCAACACCCTGCGCCTAGAGCTTCAGGACCCGAAGCCCA GTGATGAGGGGCTCTACAGCTGCTCTGCCCAGAGCCCACTGGGCAAGGTCAACACATCCCTGGCGCTTCG GCTGGAAGGTGTACGAGTGAGGATGAATCCCTCTGCTACTGTACCCGAGGGGGAGCCTGTCACAGTGACC TGCGAGGACCCTGCTGCCCTCTCACCTGCACTCTATGCCTGGTTTCACAATGGCCATTGGCTTCAGGAGG GACCAGCTTCGTCACTCCAGTTCCCAGTGACGACACAGGCTCATGCTGGCGCTTACTTTTGCCAGGTGCA TGATACTCAGGGCACGCGGAGCTCCAGACCTGCCAGCCTGCAAATTCTCTATGCCCCCCGGGATGCTGTC CTGTCTTCCTTCCGAGACTCCAGGACCAGGCACATGGTCGTGATTCAGTGCACCGTGGACAGTGAACCAC CTGCTGAGCTGGTTCTCTCCCGAAACAGCAAGGTGCTAGCTGCCAGCCGTGAGCTTCACAGCTCAGCACC CGGGATAAGCCACGTCCAGGCAGCCCGGAATGCTCTTCGACTACAGGTGCAAGACACGACTTTTGGTGAT GGCAACACTTACGTTTGCACAGCCCGGAATACACTGGGCTCCATCAGTACCACCCACAAGCTTCTGAGGG AGACTGATATACATGTGACAGCTGAGCCAGGCTTGGATGTGCCAGAGGGCACAGCTCTAAACCTGAGCTG CCACCTCCCCGGTGGCTCCCAGCCTGTGGGCAACTCTTCCTTCACGTGGTTCTGGAATCGCCATCTCCTA CATTCAGCTCCTGTGCCCACACTCTCCTTCACCCCTGTGGTCCGGGCTCAGGCTGGGTCGTACCACTGCA GGGCTGAGCTCTCCACTGGGGTTACTACCTCACCTCCCGTCATGCTCCGTGTCCTCTATCCTCCCAAGAC TCCCACTCTCACAGTGTTTGTGGAACCTCAGGGTGGCCATCAGGGCATCCTCGACTGTCGAGTGGACAGT GAGCCCCTGGCCAGTCTCACCCTCCAGCTGGGCAGTCAACTAGTAGCTTCTAACCAACCGCACAGCGCTC CCACCCAGCCCCACATCCGAGTCACTGCTCCTCCCAATGCCTTGAGACTGGACATAGAGGAGCTCGGACC TAACAATCAAGGGGAGTATGTGTGCACAGCCTCCAACGCTCTGGGCTCTGCCTCAGCCTCAGCCTACTTT GGGACCAGAGCCCTGCAGCAGCTGCAGCTGTTCCAGAGGCTGCTCTGGGTCCTGGGATTTCTGGCAGGCT TCCTGGGCCTGCTGCTGGGCCTAGGGGCCTGTTACAGCTGGAGAAAGAAGAATTCTAGCAAACTGAATGT GAATGAGAATTCAGCAGAGATGACCACTCAGAAAGATAATATCCAGGAGGAGACGGTTGCTGCTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200553 representing NM_001107777 Red=Cloning site Green=Tags(s) MCALLSLLLLASVFTPGQTTWGVSSPKDVQGLTGSCLLIPCIFSFPADVSVPNGITAIWYYDYSGKRQVV IHSGDPKLVDERFRGRAELTGNMDHKVCNLLLKDLKLEDSGTYNFRFEISDGNRWSDVRGTTVTVTKDPS PPTITIPHELREGVEVNVNCSTPYLCLQEKQVSLHWQGQDPTRSVISNFQSLEPTGVYHQTTLHMAPSWQ DHRRTLRCQLSLATHSSQKEVYLQVQHAPKGVEILLSSSGRNILPGNLVTLTCRVNSSYPAVSSVDWVKD GVNLTAKGHVLQLSSATWNDSGVYTCQATNNVGSLASPPLSLHVFMAEVKINPAGPILENETVTLLCSTP KEAPQELRYSWYKNNILLEDAHAPTLHLPAVTRADTGFYFCEVQNTQGSERSGPVSVVVRYPPLTPDLTT FLETQAGLVGIFHCSVISEPLATVVLSHGGLTLASSSGENDFNPRFSISSAPNSVRLEIRDLQPADSGEY TCSATNSLGKSMSSLDFHANDPPRKLTFTARLDLDTSGDGDGRRGILLCQVDSDPPAQLQLLHKGHVVAT SLPSMCGSCSRRMKVSRASNSLQVEIQKPVLEDEGMYLCEASNTLGNSSVSASFNAKATVVVITPSDTLL EGTEASLTCNVTQEVAVSPANFSWFRNGVLWTQGPLETVRLQPLARTDAAVYACRLLTDDGAQLSAPVVL SVQYAPDPPKLSALLDVGQGHMAVFICTVDSYPLAHLSLFRGERLLATSLEPQRLSHGRIQAKATANSLQ LEVRELGLEDSGNYHCEATNVLGSANSSLFFQVRGAWVQVSPSPELREGQAVVLSCQVPTGVPEGTSYRW FQDGRPLQESTSSILRIAAISLRQAGAYHCQAQAPDTAAASLAAPVSLHVSYTPRHVTLSALLSTGPGRY GHLVCSAQSDPPALLRLFHQNRLVASTLQGPEELASSNPRLHVAVSSNELRLEIQFTELEDGGTYTCEAT NTLGQASATADFDAQAVRVAVWPNATVQEGQQVNLSCLAWSTHQDSLSYTWYKGGQQLLGVRSISLPSVT VSDATSYRCGVGLPGHTPHLSRPVTLDVLYAPRSLQLTYLLESQGRRLALVLCTVDSRPPAQLTLSHGGQ LLASSTEASVPNTLRLELQDPKPSDEGLYSCSAQSPLGKVNTSLALRLEGVRVRMNPSATVPEGEPVTVT CEDPAALSPALYAWFHNGHWLQEGPASSLQFPVTTQAHAGAYFCQVHDTQGTRSSRPASLQILYAPRDAV LSSFRDSRTRHMVVIQCTVDSEPPAELVLSRNSKVLAASRELHSSAPGISHVQAARNALRLQVQDTTFGD GNTYVCTARNTLGSISTTHKLLRETDIHVTAEPGLDVPEGTALNLSCHLPGGSQPVGNSSFTWFWNRHLL HSAPVPTLSFTPVVRAQAGSYHCRAELSTGVTTSPPVMLRVLYPPKTPTLTVFVEPQGGHQGILDCRVDS EPLASLTLQLGSQLVASNQPHSAPTQPHIRVTAPPNALRLDIEELGPNNQGEYVCTASNALGSASASAYF GTRALQQLQLFQRLLWVLGFLAGFLGLLLGLGACYSWRKKNSSKLNVNENSAEMTTQKDNIQEETVAAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107777
ORF Size	4827 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107777.1, NP_001101247.1
RefSeq Size	6671 bp
RefSeq ORF	4830 bp
Locus ID	311426
MW	173.7 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.