Siglec1 (NM_001107777) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200553 representing NM_001107777 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGCCCTGCTCTCCTTGCTCCTGCTGGCCTCGGTCTTCACACCAGGCCAGACCACGTGGGGTGTCT CCAGTCCCAAGGATGTGCAGGGCTTGACTGGATCTTGCCTGCTCATTCCCTGCATCTTCAGCTTCCCTGC TGATGTATCAGTGCCTAATGGCATCACAGCCATCTGGTACTATGACTACTCGGGCAAACGGCAGGTGGTA ATCCACTCAGGGGACCCCAAGCTGGTGGATGAGCGCTTCAGGGGCAGAGCTGAACTGACAGGGAACATGG ATCACAAGGTGTGCAACCTGTTGCTCAAAGACCTGAAGCTTGAAGACTCAGGCACCTACAACTTCCGCTT TGAGATCAGTGATGGCAACCGCTGGTCTGATGTCAGAGGCACCACAGTCACTGTGACAAAGGATCCCAGT CCCCCCACTATTACCATCCCACATGAGCTGCGTGAAGGTGTGGAGGTGAACGTCAACTGTTCCACACCCT ACCTGTGCCTGCAGGAGAAGCAGGTCAGCCTGCATTGGCAAGGCCAGGACCCCACCCGCTCCGTCATCTC CAACTTCCAGAGCCTCGAGCCCACTGGCGTCTACCACCAGACGACCCTACATATGGCCCCATCCTGGCAG GACCACAGACGGACCCTGCGCTGCCAGCTGTCATTGGCCACGCACAGTAGTCAGAAAGAGGTTTATCTAC AAGTGCAGCATGCCCCCAAAGGTGTGGAGATCCTCCTTAGCTCCTCAGGAAGGAACATCCTTCCAGGGAA CCTGGTCACGCTCACCTGCCGGGTGAATAGCAGCTATCCTGCCGTCAGTTCTGTGGATTGGGTCAAGGAT GGAGTGAACCTCACAGCCAAGGGGCATGTGCTCCAGCTATCCTCAGCAACCTGGAATGATTCTGGGGTCT ACACCTGTCAAGCAACAAATAATGTGGGCTCTCTGGCATCACCCCCACTCAGCCTCCACGTTTTTATGGC TGAAGTCAAGATAAACCCAGCAGGGCCCATCTTGGAAAATGAGACAGTGACACTCCTCTGTAGCACCCCC AAGGAGGCACCCCAGGAGCTCCGCTACAGCTGGTACAAGAACAACATTCTTCTGGAAGACGCCCATGCCC CAACCTTGCACCTGCCTGCAGTCACCAGGGCTGATACTGGCTTCTATTTCTGTGAGGTGCAGAACACCCA GGGCAGTGAGCGCTCCGGTCCAGTGAGTGTGGTGGTCAGATATCCACCTCTTACTCCAGACCTGACCACC TTCTTGGAGACACAGGCTGGACTTGTGGGCATCTTCCATTGCTCTGTGATCAGCGAGCCCCTGGCTACTG TGGTACTGTCACACGGAGGTCTCACATTGGCCTCCAGCTCTGGAGAAAATGACTTCAACCCCCGATTCAG CATTTCCTCTGCCCCCAACTCCGTGCGCCTAGAAATCCGAGACTTGCAGCCAGCGGACAGCGGGGAGTAC ACATGCTCAGCCACCAACTCCCTTGGAAAATCAATGTCCAGCCTAGACTTCCATGCAAATGATCCCCCAA GAAAGCTCACATTCACTGCCAGGCTGGATTTGGATACCTCTGGAGACGGGGATGGACGGCGTGGCATCCT CTTGTGCCAAGTAGACAGTGATCCCCCAGCCCAGCTACAGCTTCTCCACAAAGGTCATGTTGTGGCCACT TCTCTGCCATCAATGTGTGGGAGCTGCTCTCGGCGCATGAAAGTCAGCAGAGCCTCCAACTCACTGCAGG TGGAGATCCAGAAGCCGGTACTAGAGGATGAGGGCATGTACCTGTGTGAGGCTAGCAACACACTGGGCAA CTCCTCTGTCTCAGCCTCTTTCAATGCTAAGGCTACTGTCGTGGTCATCACACCGTCAGATACACTGCTT GAAGGCACAGAAGCCAGCCTAACTTGTAACGTGACCCAGGAGGTTGCCGTCAGCCCTGCCAACTTTTCTT GGTTCCGGAATGGAGTGCTGTGGACCCAGGGACCACTGGAGACTGTGAGGCTGCAGCCTCTGGCCAGGAC TGATGCTGCCGTCTATGCCTGCCGCCTCCTCACTGACGATGGGGCCCAGCTCTCGGCTCCTGTGGTCCTA AGTGTGCAGTATGCCCCAGACCCTCCAAAGCTGTCAGCCCTCCTGGATGTGGGTCAGGGCCACATGGCTG TGTTCATCTGCACTGTGGACAGCTATCCCCTGGCTCACCTGTCTCTATTCCGTGGGGAGCGCCTCCTGGC CACCAGCTTGGAACCCCAGCGTCTATCCCATGGTAGAATCCAGGCCAAGGCCACAGCCAACTCCCTGCAG CTAGAGGTCCGGGAACTAGGCCTTGAAGACTCTGGAAACTACCACTGTGAAGCCACAAATGTTCTTGGAT CAGCCAACAGTTCACTCTTCTTCCAGGTCAGAGGAGCCTGGGTCCAGGTTTCGCCATCACCTGAGCTCCG AGAGGGCCAGGCTGTGGTCCTGAGCTGCCAGGTGCCCACAGGGGTGCCTGAGGGAACCTCATACCGCTGG TTTCAGGATGGCCGCCCCCTCCAGGAGTCAACCTCATCTATATTGCGCATTGCAGCCATAAGTCTGAGGC AAGCTGGTGCCTACCATTGCCAAGCTCAGGCTCCAGACACAGCTGCAGCCAGCCTGGCTGCCCCTGTCAG CCTCCATGTGTCCTATACCCCACGTCACGTGACACTCAGCGCCCTGCTGAGCACGGGCCCTGGGCGATAC GGCCACCTGGTGTGCAGTGCACAAAGTGACCCTCCAGCTCTGCTGCGACTGTTCCACCAGAATCGCCTCG TGGCCTCTACCCTACAAGGCCCGGAGGAATTGGCAAGCAGTAATCCCCGGCTGCATGTGGCTGTGAGCTC CAATGAGTTGCGCCTGGAGATCCAATTCACAGAGCTGGAGGATGGTGGGACCTACACATGTGAAGCCACC AACACACTGGGCCAGGCATCTGCTACAGCTGACTTCGATGCCCAGGCTGTGCGTGTGGCCGTGTGGCCCA ATGCCACTGTGCAAGAGGGGCAGCAGGTGAACCTCAGCTGCTTGGCGTGGAGCACCCACCAGGACTCACT CAGCTACACATGGTACAAGGGCGGCCAACAACTGCTTGGTGTCAGATCCATCTCCCTGCCCAGCGTTACA GTTTCGGATGCTACCTCCTACCGCTGCGGTGTGGGGCTCCCTGGCCACACACCCCATCTCTCCAGACCTG TGACCCTGGATGTCCTCTACGCTCCCCGAAGCCTGCAGCTGACCTACCTCCTGGAGAGCCAGGGCCGGCG GCTGGCCCTGGTACTGTGTACTGTGGATAGTCGCCCACCTGCCCAGCTAACTCTCAGCCACGGTGGCCAG CTCCTAGCCTCCTCCACTGAAGCTTCCGTCCCCAACACCCTGCGCCTAGAGCTTCAGGACCCGAAGCCCA GTGATGAGGGGCTCTACAGCTGCTCTGCCCAGAGCCCACTGGGCAAGGTCAACACATCCCTGGCGCTTCG GCTGGAAGGTGTACGAGTGAGGATGAATCCCTCTGCTACTGTACCCGAGGGGGAGCCTGTCACAGTGACC TGCGAGGACCCTGCTGCCCTCTCACCTGCACTCTATGCCTGGTTTCACAATGGCCATTGGCTTCAGGAGG GACCAGCTTCGTCACTCCAGTTCCCAGTGACGACACAGGCTCATGCTGGCGCTTACTTTTGCCAGGTGCA TGATACTCAGGGCACGCGGAGCTCCAGACCTGCCAGCCTGCAAATTCTCTATGCCCCCCGGGATGCTGTC CTGTCTTCCTTCCGAGACTCCAGGACCAGGCACATGGTCGTGATTCAGTGCACCGTGGACAGTGAACCAC CTGCTGAGCTGGTTCTCTCCCGAAACAGCAAGGTGCTAGCTGCCAGCCGTGAGCTTCACAGCTCAGCACC CGGGATAAGCCACGTCCAGGCAGCCCGGAATGCTCTTCGACTACAGGTGCAAGACACGACTTTTGGTGAT GGCAACACTTACGTTTGCACAGCCCGGAATACACTGGGCTCCATCAGTACCACCCACAAGCTTCTGAGGG AGACTGATATACATGTGACAGCTGAGCCAGGCTTGGATGTGCCAGAGGGCACAGCTCTAAACCTGAGCTG CCACCTCCCCGGTGGCTCCCAGCCTGTGGGCAACTCTTCCTTCACGTGGTTCTGGAATCGCCATCTCCTA CATTCAGCTCCTGTGCCCACACTCTCCTTCACCCCTGTGGTCCGGGCTCAGGCTGGGTCGTACCACTGCA GGGCTGAGCTCTCCACTGGGGTTACTACCTCACCTCCCGTCATGCTCCGTGTCCTCTATCCTCCCAAGAC TCCCACTCTCACAGTGTTTGTGGAACCTCAGGGTGGCCATCAGGGCATCCTCGACTGTCGAGTGGACAGT GAGCCCCTGGCCAGTCTCACCCTCCAGCTGGGCAGTCAACTAGTAGCTTCTAACCAACCGCACAGCGCTC CCACCCAGCCCCACATCCGAGTCACTGCTCCTCCCAATGCCTTGAGACTGGACATAGAGGAGCTCGGACC TAACAATCAAGGGGAGTATGTGTGCACAGCCTCCAACGCTCTGGGCTCTGCCTCAGCCTCAGCCTACTTT GGGACCAGAGCCCTGCAGCAGCTGCAGCTGTTCCAGAGGCTGCTCTGGGTCCTGGGATTTCTGGCAGGCT TCCTGGGCCTGCTGCTGGGCCTAGGGGCCTGTTACAGCTGGAGAAAGAAGAATTCTAGCAAACTGAATGT GAATGAGAATTCAGCAGAGATGACCACTCAGAAAGATAATATCCAGGAGGAGACGGTTGCTGCTGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107777
Insert Size	4830 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107777.1, NP_001101247.1
RefSeq Size	6671 bp
RefSeq ORF	4830 bp
Locus ID	311426

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