Unc13b (NM_022862) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Munc13-2; Unc13h2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200304 representing NM_022862 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACGACTGTTGCGGGAGTCTGAGGAAGAAATCATGCTGACACTGGGACCTTCATCCAGCCTCTCAC CAGATCAGGTCAGGACAGAGACTGTGTGTATTGTCAAGGGCAAGTCCACAGGTCCTACTGGGTCCTTGCC AGAGGACAACTTCCCACCCCCTTGCTGTGAGTCAGCAGACTCCACCACTAGTGGAGAGAGAGATAGGAAC CTGGCCCAACTTGGTAGCTTTGAGCAGCAAGCCAGTAGCCAACCTTCTCTGGCCTGCACTGCTTGTGCTT CAGGGTCTGATTCTCGGGAACTCTCGCCTGCCAGCATAACAAGCTGTTCAGAGCCTTCAGAGAGAAACAA AGCCAGGCCCATATTCCCCAGAGGTCCTGGCCAAAGATGTAGGCATGAGCATCAGGAGCCTCTGGGAGAT GTAGTAGAATATATCATCAGAGAGCTGCAAGGAATCAGTCGTCTCCAGTCTGAGATTGCTGAGCTGCAAC AGCACTTGAACCAAGTCCGGGGCTCTGTGGATGAAGTATCTAGCTGTGTAGATTCAGTGCTGAGTGAAAT AGAAGGCTTGCACGTGGGTAGCAGCTCCCTGGGCAAAGTACGCCATGGGGAAAAGGCCCAGGAACTCCAT GTAGAAAGATCCAGAGAGGAAGCTATTCTTTATTTGTATGGACTTCCTGAACATGATGGGGAAAGCACTG TGGAACTGGTAGATAACTTTCTAGCTAAGCACCTCTGTGTTAATGGCATGCAGTGCAACAGGTATGTCAG AGAGGCATACAGGGCAGGCACAGCTCCTGCCCCCCGGCCCACTGTGGTGAAACTTGTCCACCCAGAGCAC AGAGACCTCATCTTGCAAAAATCCATCCTCTTGCAGAGTGTGGGGGTCAGGGTTGCTACCAGAGAGGAAC CAGTCTGGCCAGAAGGGTGTAAGAATCCCCCAAAGGAGTCTCTCTCATGCTTGCAACAATTTCAAGACCA CAGTCGGAACCATCAGGGTAAACCAGCTCTTCAGCTAGAAACAGGGAACAGAAGACAAATGTCGGGACCA CACCAGATGAGGACTCAGAATCAACATAGAGAACTCCAGGCTTCTGAGCATCAAGGGCTTAGTTTCCTAC CAAAGGATGGCTCAGCAAAACAGAGTGATGTGTCTAAGCTGCAGGATGAAGTAAAAGGAACATCAGGAGC TCCCCAGGTAATCAGTGATCCTTGTGGTGAACTTTCTCTCCTCCATCAACTTGAGGGGTCTTCCCCAGTG TTAATCCCAAAAGAGGAAGATTGTGGAAAATTACAGATTTTCAAACAGGACAGCCAAGAGCATAAGGCAT GTAACGTGACAAAGCTACAAAGTGATTGCAACAATGCAATTAAAGCCAGCAGTTGCCTTTCATTGTCTGG ACCACTGAAAGCAGAGAAGGTAAACGCAGAGGACAGGATGCTAGGCGGTGAAGATGGACTTGATATTCTG AGCCCAAAGCAGCTGGAGGACCTTTTGGCAGACAAGTCTAGGAGGTTTGCGACTCTGAATCCTGACAGCG CAGTGGAGGAAGTGATCATAGGACCCGAGACTTTCAGTAACATGGTGCATATTGACCTGAATGAAGAAGA GACCTGTACTGCCCAGGTCCTTAAGAATGTCTTTGATAAGTCCTCATGTGTTCTGGGTGGCTCCCAGGAG GATGAAGATGTAGAAATTAAATTTCACACAACTAAGCTGAGTCGAGCGATCCACCACTTCCGTTTGGCTC TTCAGGGGGTATTTCAGAAGCTGGAGAACAATGGGTCCATCAGTCCTGAGGACCTGGAAAGCAATGAGAG TGGTTCCCAGTCAGAAAACAGTGATAGACTTCTTTGGACAGTAAGTTCAGGAGGTGCCCACGATTGTTCA GTGGAGAGTCCAGCAAGTCAGGGGAGTGAGAGCTTGCTCAGTGTGGTCTCTGGTGGAGTAGGCATCTCTG TACAGGGTGATCAGACACCTCAAGCACCAAGTAACTTCTCTCTTGCTTCTAACAACTCACCTCTTACAAA CTCATTGCTCAGTTTCCCTCTAGCCCCAGGTCTGGGAAACGAGACTTGTTCCAGGCCTGACTCTCCTAAT CAAGGCAAACTGAGTTTAGAACAAGTATGTGCAGAGACCATTTACCTTAACAAATGCATCAACAATTTTA AAAACGTCCTAAGAGAGAAAAGACTGAGGCAGAAAAAACTTTTGCAAGAGCTAGTACAGACGGCAAGTCA TCTTTCAGTAGAAGACATCCCCTCAGAAGGAAAGCGAGAAGCTCTTCAGATTTCAGATGATGGTGACCCC TCCTTGCCTCAGTGGCTCCCAGAAGGGCCAGCGGGAGGGCTCTATGGCATCGACAGCATGCCAGATCTAC GAAGAAAGAAGCCACTGCCACTTGTCAGTGATCTGGCTATGTCACTGGTCCAGTCACGGAAAGCAGGGAT TACTTCCGCTATGGCTACACGTACCTCTCTCAAGGATGAAGATCTGAAATCCCATGTGTATAAGAAAACT CTGCAGGCCTTAATCTACCCCATCTCGTGCACCACGCCCCACAACTTTGAGGTCTGGTCTGCCACGACAC CCACCTACTGCTATGAGTGTGAAGGCTTGCTCTGGGGCCTTGCCCGGCAAGGCATGCGCTGCAGCGAGTG TGGCGTCAAGTGCCATGAGAAGTGCCAAGACCTGCTCAATGCCGATTGCCTACAGCGGGCCGCTGAAAAG AGCTCTAAGCACGGAGCTGAGGACCGGACTCAGAACATTATCATGGCCATGAAGGACCGTATGAAGATCC GAGAACGGAATAAACCAGAGATCTTTGAGGTTATCCGGGATGTGTTCACAGTGAGCAAAGTTGCCCACGT GCAGCAGATGAAAACAGTAAAGCAGAGTGTCCTGGATGGCACCTCCAAGTGGTCGGCCAAAATTACCATC ACGGTGGTTTGTGCCCAGGGCCTACAAGCCAAAGACAAGACAGGATCCAGTGACCCTTATGTGACTGTGC AAGTTGGGAAAACCAAGAAGCGCACTAAGACCATTTTTGGAAATTTGAATCCTGTTTGGGAGGAGAAGTT CCATTTTGAGTGCCACAACTCTTCCGATCGAATTAAGGTACGTGTGTGGGATGAGGATGATGACATCAAA TCCAGAGTAAAGCAGCGGCTGAAGCGAGAGTCGGATGATTTCCTTGGCCAAACTATCATTGAAGTTCGAA CGCTGAGTGGAGAGATGGATGTCTGGTATAATCTGGAGAAGAGAACAGACAAGTCAGCTGTCTCAGGGGC TATCCGACTGCAAATCAATGTGGAGATCAAGGGGGAGGAGAAGGTAGCCCCATACCATGTGCAATACACA TGTCTCCATGAGAATCTTTTCCATTACCTCACAGACATTCAGGGCAGTGGAGGAGTCTGGATCCCAGACG CTCGGGGGGACGATGCATGGAAGGTGTACTTTGACGAGACAGCCCAAGAAATTGTGGATGAATTTGCCAT GCGCTATGGCATCGAGTCCATATATCAGGCCATGACGCACTTTGCGTGTTTGTCATCCAAGTACATGTGT CCTGGCGTACCAGCCGTGATGAGTACCTTACTGGCCAACATCAATGCGTATTATGCTCACACAACCGCCT CCACTAACGTCTCTGCATCTGACCGCTTTGCCGCCTCCAACTTTGGTAAAGAAAGATTTGTAAAACTGCT GGACCAGCTACACAACTCACTGAGGATAGACCTGTCTACATACAGGAATAACTTTCCTGCTGGGAGCCCT GAGCGGCTCCAGGATTTAAAATCTACAGTGGACTTGCTGACCAGCATTACTTTCTTCAGGATGAAGGTGC AAGAACTTCAGAGCCCCCCAAGAGCCAGCCAGGTGGTAAAGGACTGTGTGAAGGCCTGCTTGAACTCTAC ATATGAATACATCTTCAACAACTGCCATGACCTCTACAGTCACCAATACCAGCTGCAGGAACAACCACTA GAGGAACCAGGGCCCAGCATTCGGAACTTGGATTTCTGGCCCAAACTTATCACACTGATTGTATCAATCA TAGAGGAGGATAAGAATTCCTACACACCTGTTCTGAACCAGTTTCCTCAGGAGTTGAATGTAGGAAAAGT CAGTGCAGAAGTGATGTGGCACCTGTTTGCTCAAGACATGAAATATGCACTGGAGGAACATGAGAAAGAC CGGCTGTGTAAGAGTGCTGACTACATGAACCTGCACTTCAAGGTGAAGTGGCTCCACAATGAATATGTGC GGGAGCTGCCTGCCCTCCAGGGGCAGGTGCCTGAGTACCCAGCGTGGTTTGAGCAGTTCGTGCTACAGTG GCTAGATGAAAATGAAGACGTGTCCCTGGAATTCCTTCGTGGGGGCTTGGAACGAGATAAGAGAGATGGG TTCCAGCAAACATCGGAGCATGCCTTGTTTTCCTGTTCTGTGGTGGACGTCTTTACACAACTCAACCAGA GCTTTGAGATCATCCGGAAATTGGAATGTCCAGACCCCAGCATCCTTGCCCATTATATGAGAAGGTTTGC TAAGACCATCGGCAAAGTGCTGATACAGTATGCAGACATCTTATCAAAGAACTTCCCAGCTTACTGCACA AAGGAGAGACTGCCCTGTATCCTGATGAACAACATGCAGCAACTGAGGGTCCAGCTGGAGAAAATGTTTG AGGCCATGGGAGGCAAAGAGCTGGACTCTGAAGCTGCAGACAGTCTGAAGGAGCTGCAGGTGAAACTGAA TACAGTTCTGGATGAGCTCAGCATGGTGTTTGGAAACAGCTTCCAGGTGCGGATCGACGAGTGTGTTCGA CAAATGGCTGACATCCTGGGCCAAGTACGGGGCACAGGGAATGCATCCCCTAACGCCAGAGCCTCGGTGG CTCAGGATGCAGATAGTGTGCTGCGACCTCTCATGGATTTCCTGGATGGCAACCTCACACTGTTTGCCAC TGTGTGTGAGAAGACAGTGCTGAAGCGAGTCCTGAAGGAGCTCTGGCGAGTGGTGATGAACACCATGGAG CGGGTCATCGTCCTGCCCCCACTCATTGACCAGACAGGCACCCAGCTGATCTTAACTGCTGCCAAGGAGC TGAGCCAGCTTTCCAAACTCAAGGACCACATGGTACGAGAGGAAACACGGAATCTCACTCCAAAGCAGTG TGCTGTCCTCGACTTGGCCTTGGACACCGTCAAGCAATACTTCCATGCAGGAGGCAATGGTCTGAAGAAA ACCTTCCTGGAAAAGAGCCCAGACCTGCAGTCTCTACGTTATGCCCTCTCTCTGTATACACAGACCACAG ACACTCTCATCAAGACATTTGTGCGCTCACAGACTGCCCAAGTACATGATGGGAAAGGTATTAGGTTTAC TGCTAACGAGGACATTCGGCCAGAAAAGGGGGCTGGTGTGGACGACCCTGTGGGAGAAGTCTCTATTCAG GTGGACTTGTTTACACATCCTGGCACTGGGGAGCATAAGGTCACAGTGAAAGTGGTAGCAGCCAATGATC TCAAGTGGCAGACAGCAGGTATGTTCCGGCCTTTTGTGGAAGTGACCATGGTTGGCCCACACCAAAGCGA TAAGAAGAGGAAGTTCACAACCAAGTCTAAAAGTAACAGCTGGACTCCCAAGTACAACGAGACTTTTCAT TTCCTCCTGGGAAATGAAGAGGGTCCCGAGGCCTATGAATTGCAGATATGCGTGAAGGATTACTGCTTTG CCCGGGAGGATCGTGTGATAGGACTGGCAGTGATGCCCCTGAGGGATGTGGCAGCTAAGGGCAGCTGTGC CTGCTGGTGCCCGTTGGGCCGGAAGATCCACATGGATGAGACAGGCATGACCATTCTCCGGATTCTGTCT CAGAGGAGCAATGATGAGGTAGCCCGAGAGTTCGTGAAGCTCAAGTCAGAGTCTCGATCCATAGAGGAAG GGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200304 representing NM_022862 Red=Cloning site Green=Tags(s) MKRLLRESEEEIMLTLGPSSSLSPDQVRTETVCIVKGKSTGPTGSLPEDNFPPPCCESADSTTSGERDRN LAQLGSFEQQASSQPSLACTACASGSDSRELSPASITSCSEPSERNKARPIFPRGPGQRCRHEHQEPLGD VVEYIIRELQGISRLQSEIAELQQHLNQVRGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVRHGEKAQELH VERSREEAILYLYGLPEHDGESTVELVDNFLAKHLCVNGMQCNRYVREAYRAGTAPAPRPTVVKLVHPEH RDLILQKSILLQSVGVRVATREEPVWPEGCKNPPKESLSCLQQFQDHSRNHQGKPALQLETGNRRQMSGP HQMRTQNQHRELQASEHQGLSFLPKDGSAKQSDVSKLQDEVKGTSGAPQVISDPCGELSLLHQLEGSSPV LIPKEEDCGKLQIFKQDSQEHKACNVTKLQSDCNNAIKASSCLSLSGPLKAEKVNAEDRMLGGEDGLDIL SPKQLEDLLADKSRRFATLNPDSAVEEVIIGPETFSNMVHIDLNEEETCTAQVLKNVFDKSSCVLGGSQE DEDVEIKFHTTKLSRAIHHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCS VESPASQGSESLLSVVSGGVGISVQGDQTPQAPSNFSLASNNSPLTNSLLSFPLAPGLGNETCSRPDSPN QGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGKREALQISDDGDP SLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEDLKSHVYKKT LQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEK SSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITI TVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIK SRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQINVEIKGEEKVAPYHVQYT CLHENLFHYLTDIQGSGGVWIPDARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMC PGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSP ERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPL EEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKD RLCKSADYMNLHFKVKWLHNEYVRELPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGGLERDKRDG FQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPSILAHYMRRFAKTIGKVLIQYADILSKNFPAYCT KERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVR QMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTME RVIVLPPLIDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTVKQYFHAGGNGLKK TFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQ VDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNSWTPKYNETFH FLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILS QRSNDEVAREFVKLKSESRSIEEGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022862
ORF Size	5955 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022862.1, NP_074053.1
RefSeq Size	6599 bp
RefSeq ORF	5958 bp
Locus ID	64830
UniProt ID	Q62769
MW	222.1 kDa
Gene Summary	brain specific protein that may have a role in neurotransmitter release [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.