Unc13b (NM_022862) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Munc13-2; Unc13h2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200304 representing NM_022862 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACGACTGTTGCGGGAGTCTGAGGAAGAAATCATGCTGACACTGGGACCTTCATCCAGCCTCTCAC CAGATCAGGTCAGGACAGAGACTGTGTGTATTGTCAAGGGCAAGTCCACAGGTCCTACTGGGTCCTTGCC AGAGGACAACTTCCCACCCCCTTGCTGTGAGTCAGCAGACTCCACCACTAGTGGAGAGAGAGATAGGAAC CTGGCCCAACTTGGTAGCTTTGAGCAGCAAGCCAGTAGCCAACCTTCTCTGGCCTGCACTGCTTGTGCTT CAGGGTCTGATTCTCGGGAACTCTCGCCTGCCAGCATAACAAGCTGTTCAGAGCCTTCAGAGAGAAACAA AGCCAGGCCCATATTCCCCAGAGGTCCTGGCCAAAGATGTAGGCATGAGCATCAGGAGCCTCTGGGAGAT GTAGTAGAATATATCATCAGAGAGCTGCAAGGAATCAGTCGTCTCCAGTCTGAGATTGCTGAGCTGCAAC AGCACTTGAACCAAGTCCGGGGCTCTGTGGATGAAGTATCTAGCTGTGTAGATTCAGTGCTGAGTGAAAT AGAAGGCTTGCACGTGGGTAGCAGCTCCCTGGGCAAAGTACGCCATGGGGAAAAGGCCCAGGAACTCCAT GTAGAAAGATCCAGAGAGGAAGCTATTCTTTATTTGTATGGACTTCCTGAACATGATGGGGAAAGCACTG TGGAACTGGTAGATAACTTTCTAGCTAAGCACCTCTGTGTTAATGGCATGCAGTGCAACAGGTATGTCAG AGAGGCATACAGGGCAGGCACAGCTCCTGCCCCCCGGCCCACTGTGGTGAAACTTGTCCACCCAGAGCAC AGAGACCTCATCTTGCAAAAATCCATCCTCTTGCAGAGTGTGGGGGTCAGGGTTGCTACCAGAGAGGAAC CAGTCTGGCCAGAAGGGTGTAAGAATCCCCCAAAGGAGTCTCTCTCATGCTTGCAACAATTTCAAGACCA CAGTCGGAACCATCAGGGTAAACCAGCTCTTCAGCTAGAAACAGGGAACAGAAGACAAATGTCGGGACCA CACCAGATGAGGACTCAGAATCAACATAGAGAACTCCAGGCTTCTGAGCATCAAGGGCTTAGTTTCCTAC CAAAGGATGGCTCAGCAAAACAGAGTGATGTGTCTAAGCTGCAGGATGAAGTAAAAGGAACATCAGGAGC TCCCCAGGTAATCAGTGATCCTTGTGGTGAACTTTCTCTCCTCCATCAACTTGAGGGGTCTTCCCCAGTG TTAATCCCAAAAGAGGAAGATTGTGGAAAATTACAGATTTTCAAACAGGACAGCCAAGAGCATAAGGCAT GTAACGTGACAAAGCTACAAAGTGATTGCAACAATGCAATTAAAGCCAGCAGTTGCCTTTCATTGTCTGG ACCACTGAAAGCAGAGAAGGTAAACGCAGAGGACAGGATGCTAGGCGGTGAAGATGGACTTGATATTCTG AGCCCAAAGCAGCTGGAGGACCTTTTGGCAGACAAGTCTAGGAGGTTTGCGACTCTGAATCCTGACAGCG CAGTGGAGGAAGTGATCATAGGACCCGAGACTTTCAGTAACATGGTGCATATTGACCTGAATGAAGAAGA GACCTGTACTGCCCAGGTCCTTAAGAATGTCTTTGATAAGTCCTCATGTGTTCTGGGTGGCTCCCAGGAG GATGAAGATGTAGAAATTAAATTTCACACAACTAAGCTGAGTCGAGCGATCCACCACTTCCGTTTGGCTC TTCAGGGGGTATTTCAGAAGCTGGAGAACAATGGGTCCATCAGTCCTGAGGACCTGGAAAGCAATGAGAG TGGTTCCCAGTCAGAAAACAGTGATAGACTTCTTTGGACAGTAAGTTCAGGAGGTGCCCACGATTGTTCA GTGGAGAGTCCAGCAAGTCAGGGGAGTGAGAGCTTGCTCAGTGTGGTCTCTGGTGGAGTAGGCATCTCTG TACAGGGTGATCAGACACCTCAAGCACCAAGTAACTTCTCTCTTGCTTCTAACAACTCACCTCTTACAAA CTCATTGCTCAGTTTCCCTCTAGCCCCAGGTCTGGGAAACGAGACTTGTTCCAGGCCTGACTCTCCTAAT CAAGGCAAACTGAGTTTAGAACAAGTATGTGCAGAGACCATTTACCTTAACAAATGCATCAACAATTTTA AAAACGTCCTAAGAGAGAAAAGACTGAGGCAGAAAAAACTTTTGCAAGAGCTAGTACAGACGGCAAGTCA TCTTTCAGTAGAAGACATCCCCTCAGAAGGAAAGCGAGAAGCTCTTCAGATTTCAGATGATGGTGACCCC TCCTTGCCTCAGTGGCTCCCAGAAGGGCCAGCGGGAGGGCTCTATGGCATCGACAGCATGCCAGATCTAC GAAGAAAGAAGCCACTGCCACTTGTCAGTGATCTGGCTATGTCACTGGTCCAGTCACGGAAAGCAGGGAT TACTTCCGCTATGGCTACACGTACCTCTCTCAAGGATGAAGATCTGAAATCCCATGTGTATAAGAAAACT CTGCAGGCCTTAATCTACCCCATCTCGTGCACCACGCCCCACAACTTTGAGGTCTGGTCTGCCACGACAC CCACCTACTGCTATGAGTGTGAAGGCTTGCTCTGGGGCCTTGCCCGGCAAGGCATGCGCTGCAGCGAGTG TGGCGTCAAGTGCCATGAGAAGTGCCAAGACCTGCTCAATGCCGATTGCCTACAGCGGGCCGCTGAAAAG AGCTCTAAGCACGGAGCTGAGGACCGGACTCAGAACATTATCATGGCCATGAAGGACCGTATGAAGATCC GAGAACGGAATAAACCAGAGATCTTTGAGGTTATCCGGGATGTGTTCACAGTGAGCAAAGTTGCCCACGT GCAGCAGATGAAAACAGTAAAGCAGAGTGTCCTGGATGGCACCTCCAAGTGGTCGGCCAAAATTACCATC ACGGTGGTTTGTGCCCAGGGCCTACAAGCCAAAGACAAGACAGGATCCAGTGACCCTTATGTGACTGTGC AAGTTGGGAAAACCAAGAAGCGCACTAAGACCATTTTTGGAAATTTGAATCCTGTTTGGGAGGAGAAGTT CCATTTTGAGTGCCACAACTCTTCCGATCGAATTAAGGTACGTGTGTGGGATGAGGATGATGACATCAAA TCCAGAGTAAAGCAGCGGCTGAAGCGAGAGTCGGATGATTTCCTTGGCCAAACTATCATTGAAGTTCGAA CGCTGAGTGGAGAGATGGATGTCTGGTATAATCTGGAGAAGAGAACAGACAAGTCAGCTGTCTCAGGGGC TATCCGACTGCAAATCAATGTGGAGATCAAGGGGGAGGAGAAGGTAGCCCCATACCATGTGCAATACACA TGTCTCCATGAGAATCTTTTCCATTACCTCACAGACATTCAGGGCAGTGGAGGAGTCTGGATCCCAGACG CTCGGGGGGACGATGCATGGAAGGTGTACTTTGACGAGACAGCCCAAGAAATTGTGGATGAATTTGCCAT GCGCTATGGCATCGAGTCCATATATCAGGCCATGACGCACTTTGCGTGTTTGTCATCCAAGTACATGTGT CCTGGCGTACCAGCCGTGATGAGTACCTTACTGGCCAACATCAATGCGTATTATGCTCACACAACCGCCT CCACTAACGTCTCTGCATCTGACCGCTTTGCCGCCTCCAACTTTGGTAAAGAAAGATTTGTAAAACTGCT GGACCAGCTACACAACTCACTGAGGATAGACCTGTCTACATACAGGAATAACTTTCCTGCTGGGAGCCCT GAGCGGCTCCAGGATTTAAAATCTACAGTGGACTTGCTGACCAGCATTACTTTCTTCAGGATGAAGGTGC AAGAACTTCAGAGCCCCCCAAGAGCCAGCCAGGTGGTAAAGGACTGTGTGAAGGCCTGCTTGAACTCTAC ATATGAATACATCTTCAACAACTGCCATGACCTCTACAGTCACCAATACCAGCTGCAGGAACAACCACTA GAGGAACCAGGGCCCAGCATTCGGAACTTGGATTTCTGGCCCAAACTTATCACACTGATTGTATCAATCA TAGAGGAGGATAAGAATTCCTACACACCTGTTCTGAACCAGTTTCCTCAGGAGTTGAATGTAGGAAAAGT CAGTGCAGAAGTGATGTGGCACCTGTTTGCTCAAGACATGAAATATGCACTGGAGGAACATGAGAAAGAC CGGCTGTGTAAGAGTGCTGACTACATGAACCTGCACTTCAAGGTGAAGTGGCTCCACAATGAATATGTGC GGGAGCTGCCTGCCCTCCAGGGGCAGGTGCCTGAGTACCCAGCGTGGTTTGAGCAGTTCGTGCTACAGTG GCTAGATGAAAATGAAGACGTGTCCCTGGAATTCCTTCGTGGGGGCTTGGAACGAGATAAGAGAGATGGG TTCCAGCAAACATCGGAGCATGCCTTGTTTTCCTGTTCTGTGGTGGACGTCTTTACACAACTCAACCAGA GCTTTGAGATCATCCGGAAATTGGAATGTCCAGACCCCAGCATCCTTGCCCATTATATGAGAAGGTTTGC TAAGACCATCGGCAAAGTGCTGATACAGTATGCAGACATCTTATCAAAGAACTTCCCAGCTTACTGCACA AAGGAGAGACTGCCCTGTATCCTGATGAACAACATGCAGCAACTGAGGGTCCAGCTGGAGAAAATGTTTG AGGCCATGGGAGGCAAAGAGCTGGACTCTGAAGCTGCAGACAGTCTGAAGGAGCTGCAGGTGAAACTGAA TACAGTTCTGGATGAGCTCAGCATGGTGTTTGGAAACAGCTTCCAGGTGCGGATCGACGAGTGTGTTCGA CAAATGGCTGACATCCTGGGCCAAGTACGGGGCACAGGGAATGCATCCCCTAACGCCAGAGCCTCGGTGG CTCAGGATGCAGATAGTGTGCTGCGACCTCTCATGGATTTCCTGGATGGCAACCTCACACTGTTTGCCAC TGTGTGTGAGAAGACAGTGCTGAAGCGAGTCCTGAAGGAGCTCTGGCGAGTGGTGATGAACACCATGGAG CGGGTCATCGTCCTGCCCCCACTCATTGACCAGACAGGCACCCAGCTGATCTTAACTGCTGCCAAGGAGC TGAGCCAGCTTTCCAAACTCAAGGACCACATGGTACGAGAGGAAACACGGAATCTCACTCCAAAGCAGTG TGCTGTCCTCGACTTGGCCTTGGACACCGTCAAGCAATACTTCCATGCAGGAGGCAATGGTCTGAAGAAA ACCTTCCTGGAAAAGAGCCCAGACCTGCAGTCTCTACGTTATGCCCTCTCTCTGTATACACAGACCACAG ACACTCTCATCAAGACATTTGTGCGCTCACAGACTGCCCAAGTACATGATGGGAAAGGTATTAGGTTTAC TGCTAACGAGGACATTCGGCCAGAAAAGGGGGCTGGTGTGGACGACCCTGTGGGAGAAGTCTCTATTCAG GTGGACTTGTTTACACATCCTGGCACTGGGGAGCATAAGGTCACAGTGAAAGTGGTAGCAGCCAATGATC TCAAGTGGCAGACAGCAGGTATGTTCCGGCCTTTTGTGGAAGTGACCATGGTTGGCCCACACCAAAGCGA TAAGAAGAGGAAGTTCACAACCAAGTCTAAAAGTAACAGCTGGACTCCCAAGTACAACGAGACTTTTCAT TTCCTCCTGGGAAATGAAGAGGGTCCCGAGGCCTATGAATTGCAGATATGCGTGAAGGATTACTGCTTTG CCCGGGAGGATCGTGTGATAGGACTGGCAGTGATGCCCCTGAGGGATGTGGCAGCTAAGGGCAGCTGTGC CTGCTGGTGCCCGTTGGGCCGGAAGATCCACATGGATGAGACAGGCATGACCATTCTCCGGATTCTGTCT CAGAGGAGCAATGATGAGGTAGCCCGAGAGTTCGTGAAGCTCAAGTCAGAGTCTCGATCCATAGAGGAAG GGAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022862
Insert Size	5958 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022862.1, NP_074053.1
RefSeq Size	6599 bp
RefSeq ORF	5958 bp
Locus ID	64830
UniProt ID	Q62769
Gene Summary	brain specific protein that may have a role in neurotransmitter release [RGD, Feb 2006] Transcript Variant: This variant (1) encodes the longer isoform (b).

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