Cdk12 (NM_001033867) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Crk7; Crkrs; Pksc
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200174 representing NM_001033867 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAATTCAGAGAGACATGGGGGCAAGAAGGACGGGAGTGGAGGAGCTTCTGGAACTTCGCAGCCGT CATCGGGAGGCGGCAGCTCCAACAGCAGGGAGCGTCACCGCTTGGTGTCGAAGCACAAGCGGCATAAGTC CAAGCACTCCAAAGACATGGGGTTGGTAACCCCCGAAGCGGCATCTTTGGGTACAATAATCAAACCATTG GTGGAGTATGATGACATCAGCTCTGATTCAGACACCTTCTCAGATGACATGGCCTTCAAATCAGACAGGA GGGAGAACGATGAACGTCGTGGAACGGATCGGAGTGACCGCCTGCACAGACATCGTCACCACCAGCACCG GCGGTCCCGAGACTTGTTAAAAACTAAACAGACCGAAAAGGAAAAAAACCAGGAAGTCTCCAAATCTGGA TCGATGAAGGACCGGTTATCAGGTAGTTCGAAACGTTCAGTGGAGGGGAACGATGATTATGGGAAGGCAC AATTATCCAAAAGCAGCAGCAAGGAATCCAGGTCGTCCAAAATGCACAAAGAGAAGACCCGGAAAGAACG AGAGTTAAAGTCTGGACACAAGGACCGTAGTAAAAGTCATCGGAAAAGGGAAACACCCAAAAGTTACAAA ACGGTGGATAGCCCCAAACGGAGATCTAGGAGTCCCCATAGGAAATGGTCTGACAGTTCCAAGCAAGATG ACAGCCCTTCTGGAGCTTCTTATGGCCAAGATTACGATCTTAGCCCCCCAAGGTCTCATACTTCTAGCAA TTATGACTCCTACAAGAAGAGTCCGGGAAGTACCTCAAGAAGGCAGTCAATCAGCCCACCTTATAAAGAG CCTTCTGCTTACCAGTCCAGCACTCGGTCACCCAGTCCCTACAGCCGACGACAGAGGTCAGTGAGCCCCT ATAGCCGGAGACGGTCATCCAGCTATGAAAGGAGCGGTTCTTACAGCGGGAGATCACCCAGCCCCTATGG CCGAAGGCGATCTAGCAGCCCTTTCTTGAGCAAGAGGTCTCTGAGTCGGAGTCCAATCTCCAGTAGGAAA TCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACACTCATCTTCACATAGTAAAAAGAAGCGAT CCGGGTCACGAAGTCGTCATTCCAGCATCTCACCTGTCAGGCTTCCATTGAATTCCAGCCTGGGAGCTGA ACTCAGTAGAAAAAAGAAGGAAAGAGCAGCTGCTGCAGCAGCAGCAAAATTGGATGGAAAAGAATCTAAG GGTTCACCTATAATTTTGCCTAAAAAAGAGAAATTTGAGGTGAAGGAGTCAGGGTTAGAGTCTAAAAAGT TACCCAGAGGTATAAAGTCAGAAAAATCTACCCCAGATACTGAACTGGTAAATGTAGCACATTCAAACAC AGAGGTAAAAAATTGTTTAGATACAGGGAAAGTAAAGTTGGATGAGAACTTGCAGAAGCATCCTGTTAAG GATTTGAAAGCACAGGGAACAAAGGACACCAAACCTGTAGCACTGAAGGAGGTGATTGTTACTTCAAAGG AGACAGAGACATCAGAAAAGGAGGCCCTTCCACCTCTCCCCACAATAACTTCTCCACCCCCTTTACCATC TACTACTCCTCCACCTCAGACACCCCCTTTGCCACCTTTGCCTCCACTACCAGCTGTTCCACTGCAGCCA CCTTTGCCTCCTCCCCAACCACCATTTAGTCAAGTTCCTGTTTCAAATACTTCAACTTTACCCTCTTCTC CTCACCCAAGGACATCTACTTTATCCTCTCAGACAAATTCTCAGCCCCTTGTACAGGTTTCTATGAAGAC TCAATTATCTGTAACAGCTGCTATTCCACACCTGAAAACTTCAACATTGCCTCCTCTGCCCCTCCCTCCC CTATTACCTGGAGATGATGACATGGATAGTCCAAAAGAAATGCTTCCTTCAAAGCCTGCAAAGAAAGAGA AAGAGCAGAGGACTCGCCACTTACTCACAGACTTGCCTCTTCCTCCTGAGCTACCAGGTGGAGATCCATC TCCCCCAGATTCTCCAGAGCCAAAGGCAATTACACCACCTCAACAACCATATAAAAAGAGACCAAAAATT TGTTGTCCACGTTATGGAGAAAGAAGACAAACAGAAAGTGATTGGGGGAAGCGCTGCGTGGACAAGTTTG ACATTATTGGGATTATTGGAGAAGGAACCTATGGCCAAGTATATAAAGCCAAGGACAAAGACACAGGAGA ACTTGTAGCTCTGAAGAAGGTTCGGCTAGACAATGAGAAAGAGGGCTTCCCAATCACAGCCATCCGGGAG ATTAAGATTCTTCGTCAGTTAGTTCATCAAAGTGTTGTAAACATGAAGGAGATTGTCACAGACAAACAAG ATGCACTGGATTTCAAGAAGGACAAAGGTGCCTTTTACCTTGTATTTGAATATATGGACCATGACTTAAT GGGACTGCTAGAATCAGGTTTGGTGCACTTTTCTGAGGACCATATCAAGTCATTCATGAAACAGCTAATG GAAGGACTGGATTACTGTCACAAAAAGAATTTCCTCCATCGGGATATTAAATGTTCTAACATTTTGCTGA ATAACAGTGGGCAAATCAAACTGGCAGATTTTGGCCTTGCTCGGCTCTATAACTCTGAAGAGAGTCGCCC TTACACAAACAAAGTCATTACTCTGTGGTATCGACCTCCAGAGCTGCTTCTTGGAGAGGAAAGATACACA CCAGCCATTGATGTTTGGAGCTGTGGGTGCATCCTTGGGGAACTGTTCACAAAGAAACCTATTTTTCAAG CCAATTTGGAACTGGCTCAGCTAGAACTGATCAGTCGTCTCTGTGGTAGTCCTTGTCCAGCAGTGTGGCC TGATGTTATCAAGCTGCCCTACTTCAACACCATGAAACCGAAAAAGCAATACAGGAGACGCCTAAGAGAA GAATTCTCTTTCATTCCTTCAGCGGCACTTGATCTGTTGGACCACATGCTGACACTGGATCCTAGCAAGC GCTGCACAGCGGAACAAACCCTACAGAGTGACTTTCTTAAAGATGTGGAACTCAGCAAAATGGCACCTCC AGACCTACCTCACTGGCAGGATTGCCATGAATTGTGGAGTAAGAAACGTCGACGGCAACGACAAAGTGGT ATTGTGATAGAAGAGCAACCTCCATCCAAAGCTTCTCGAAAAGAAACTACCTCAGGGACAGCAGCTGAGC CTGTGAAGAACAGTAGCCCAGCACCACCTCAACCTGCTCCTGTCAAGGCAGAGCCTGGTCCAGGGGATGC AGTAGGGCTTGGTGACATCACACAGCAGTTGAATCAAAGTGAATTGGCAGTGTTATTAAACCTGCTTCAG AGCCAAACTGACCTGAGCATCCCTCAGATGGCACAATTGCTTAACATCCACTCCAATCCAGAGATGCAAC AGCAGCTTGAAGCCCTGAATCAGTCTATTAGTGCACTGACTGAAGCCAGTTCCCAGCAGCAAGACTCAGA ATCCATAGCCCCAGAGGAATCATTGAAGGAGGTACCTTCTGTATCTGTGGTCCTGCCTCCTGCTGAACAG ACAACTCCTGAAGCTTCAAACACACCAGCTGACATGCAGAATATGTTGGCAGTTCTCTTGAGTCAGCTGA TGAAAACCCAAGAGCCAGCAGGTAACCTGGAGGAAAACACCAGTGACAAGAATAGTGGGCCACAGGGGCC CCGAAGAACCCCTACAATGCCACAGGAGGAGGCAGCAGCATGTCCTCCTCACATTCTTCCACCAGAGAAG AGGCCCCCTGAACCCCCTGGACCTCCACCGCCGCCACCTCCACCCCCTCTGGTTGAAGGCGATCTTTCCA GCGCCCCCCAGGAGTTGAATCCCGCCGTGACAGCCGCCTTGCTGCAACTTTTATCCCAGCCTGAAGCAGA GCCTCCTGGCCACCTGCCACATGAGCACCAGGCCTTGAGACCAATGGAGTACTCCACTCGATCCCATCCA AACAGGACTTACGGAAATACTGATGGGCCTGAAACAGGGTTCAGTGCCACTGACACTGATGAACGCAGTT CTGGTCCAGCCTTGACAGAATCTTTGGTTCAGACCCTGGTGAAGAACAGGACCTTCTCAGGCTCTGTGAG CCACCTTGGGGAGTCCAACAGTTACCAGGGCACAGGGTCAGTGCAGTTCCCAGGGGACCAGGACCTCCGT TTTACTAGGGTTCCCTTAGCATTACACTCAGTGGTTGGGCAACCATTCCTGAAGTCTGAGGGAAATAGCA ACTCTGTGGTACATGCAGAGACCAAATTGCAAAACTATGGGGAGCTGGGACCAGGAACCACTGGGGCCAA CAGCTCAGGAACAACCCTTCAGTGGGGGGGCCCAGCTCAGTCTTTTGGAAAACCCTACCGAGGGGCTGCA AGAGTCCCACCACGAGGGGGAAGAGGGAGAGGGGTTCCTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200174 representing NM_001033867 Red=Cloning site Green=Tags(s) MPNSERHGGKKDGSGGASGTSQPSSGGGSSNSRERHRLVSKHKRHKSKHSKDMGLVTPEAASLGTIIKPL VEYDDISSDSDTFSDDMAFKSDRRENDERRGTDRSDRLHRHRHHQHRRSRDLLKTKQTEKEKNQEVSKSG SMKDRLSGSSKRSVEGNDDYGKAQLSKSSSKESRSSKMHKEKTRKERELKSGHKDRSKSHRKRETPKSYK TVDSPKRRSRSPHRKWSDSSKQDDSPSGASYGQDYDLSPPRSHTSSNYDSYKKSPGSTSRRQSISPPYKE PSAYQSSTRSPSPYSRRQRSVSPYSRRRSSSYERSGSYSGRSPSPYGRRRSSSPFLSKRSLSRSPISSRK SMKSRSRSPAYSRHSSSHSKKKRSGSRSRHSSISPVRLPLNSSLGAELSRKKKERAAAAAAAKLDGKESK GSPIILPKKEKFEVKESGLESKKLPRGIKSEKSTPDTELVNVAHSNTEVKNCLDTGKVKLDENLQKHPVK DLKAQGTKDTKPVALKEVIVTSKETETSEKEALPPLPTITSPPPLPSTTPPPQTPPLPPLPPLPAVPLQP PLPPPQPPFSQVPVSNTSTLPSSPHPRTSTLSSQTNSQPLVQVSMKTQLSVTAAIPHLKTSTLPPLPLPP LLPGDDDMDSPKEMLPSKPAKKEKEQRTRHLLTDLPLPPELPGGDPSPPDSPEPKAITPPQQPYKKRPKI CCPRYGERRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIRE IKILRQLVHQSVVNMKEIVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLM EGLDYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEESRPYTNKVITLWYRPPELLLGEERYT PAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLRE EFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFLKDVELSKMAPPDLPHWQDCHELWSKKRRRQRQSG IVIEEQPPSKASRKETTSGTAAEPVKNSSPAPPQPAPVKAEPGPGDAVGLGDITQQLNQSELAVLLNLLQ SQTDLSIPQMAQLLNIHSNPEMQQQLEALNQSISALTEASSQQQDSESIAPEESLKEVPSVSVVLPPAEQ TTPEASNTPADMQNMLAVLLSQLMKTQEPAGNLEENTSDKNSGPQGPRRTPTMPQEEAAACPPHILPPEK RPPEPPGPPPPPPPPPLVEGDLSSAPQELNPAVTAALLQLLSQPEAEPPGHLPHEHQALRPMEYSTRSHP NRTYGNTDGPETGFSATDTDERSSGPALTESLVQTLVKNRTFSGSVSHLGESNSYQGTGSVQFPGDQDLR FTRVPLALHSVVGQPFLKSEGNSNSVVHAETKLQNYGELGPGTTGANSSGTTLQWGGPAQSFGKPYRGAA RVPPRGGRGRGVPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001033867
ORF Size	4452 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001033867.1, NP_001029039.1
RefSeq Size	5502 bp
RefSeq ORF	4455 bp
Locus ID	192350
UniProt ID	Q3MJK5
MW	163.8 kDa
Gene Summary	human homolog may play a role in regulation of transcription and splicing [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.