Tdrd5 (NM_001134740) Rat Tagged Lenti ORF Clone

CAT#: RR200143L4

  • LentiORF®

Lenti ORF clone of Tdrd5 (mGFP-tagged ORF) - Rat tudor domain containing 5 (Tdrd5), transcript variant 2, (10 ug)



  "NM_001134740" in other vectors (3)

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CNY 15,870.00


货期*
11周

规格
    • 10 ug

Product images

经常一起买 (4)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
    • 10 reactions

CNY 5,420.00


Lenti ORF control particles of pLenti-C-mGFP-P2A-Puro, >1x10^7 TU/ml, 0.5 ml
    • 500 ul

CNY 2,950.00


Forward primer to sequence targets cloned in expression vectors.
    • 1 nmol

CNY 570.00


Reverse primer to sequence targets cloned in expression vectors.
    • 1 nmol

CNY 570.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag mGFP
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RR200143).
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001134740
ORF Size 2985 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001134740.1, NP_001128212.1
RefSeq Size 3303 bp
RefSeq ORF 2988 bp
Locus ID 289129
UniProt ID B4F7C4
Gene Summary This gene encodes a member of the tudor domain-containing protein family. Tudor domain-containing proteins regulate a variety of processes including RNA metabolism, histone modification, and the DNA damage response. The tudor domain commonly functions to link methylated arginine or lysine marks to downstream effector proteins. In mouse, this gene is expressed in the primordial germ cells and in male germ cells during embryogenesis. In addition, it is expressed in adult testis cells where the protein is a component of the intermitochondrial cement and chromatoid bodies. Loss-of-function mutations in mouse result in arrest of spermatogenesis. [provided by RefSeq, Mar 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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