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Usp4 (NM_001135012) Rat Tagged ORF Clone
CAT＃: RR200138
- TrueORF®
Usp4 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), transcript variant 1, (10 ug)
"NM_001135012" in other vectors (3)
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CNY 12,350.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200138 representing NM_001135012
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGGCCGGGGCACCCATGAGCGACCGGATGTGGAGACCCAGAAGACGGAGCTCGGAGCCTTGA TGGGGACCACGCTCCAGCGTGGGGCTCAGTGGTATCTGATCGACAGCCGGTGGTTCAAGCAGTGGAAGAA GTACGTCGGCTTTGACAGCTGGGACATGTACAACGTGGGGGAGCACAATCTCTTTCCTGGACCGATTGAC AACTCTGGGCTCTTCTCAGATCCTGAGAGTCAGACCTTGAAGGAACACTTAATCGATGAGCTGGACTATG TGTTGGTTCCAACTGAAGCCTGGAATAAATTGCTAAATTGGTATGGCTGTGTGGAAGGCCAGCAGCCTAT TGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTGGAAGTGTATTTGCTGGAGTTG AAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTAAGTTGCCATTTTAGCAAAGCAGATACCATTGCAA CTATTGAGAAGGAGATGAGGAAGCTCTTCAACATCCCTGCAGAGCGTGAAACACGACTTTGGAACAAATA CATGAGCAACACCTATGAGCAGTTGAGCAAGCTAGACAACACTATCCAGGATGCTGGGCTGTACCAGGGT CAGGTGCTAGTAATTGAGCCCCAAAATGAAGACGGCACATGGCCCAGGCAGACCCTGCAATCAAAATCAA GCACTGCACCTAGCAGAAATTTTACTACCTCTTCAAAACCATCCGCAAGTCCCTATTCCTCAATGTCTGC CTCTCTCATTGCAAATGGTGATAGCACTAACAGCTCTGGGATGCACAACTCCGGTGTCAGCAGGGGTGGA GCTGGCTTCTCTGCCTCGTATAATTGCCAGGAGCCCCCATCACCTCATATCCAGCCTGGCCTCTGTGGAC TCGGAAACCTGGGAAACACCTGTTTTATGAATTCTGCTTTGCAGTGCCTGAGCAACACTGGTCCACTGAC TGAGTACTTTCTCAAAGATGAGTATGAGGCCGAGATCAACAGAGACAACCCTCTGGGGATGAAAGGGGAG ATTGCAGAGGCCTATGCAGAGCTCATCAAGCAGATGTGGTCTGGAAGGGACACCCACGTAGCACCACGGA TGTTCAAAACGCAAGTGGGACGTTTCGCCCCTCAGTTTTCTGGCTACCAACAACAAGACTCTCAAGAGCT GTTAGCCTTTATTCTAGATGGATTGCATGAAGACCTGAACCGAGTAAAGAAGAAGCCTTACCTGGAGCCA AAGGATGCCAATGGGCGACCAGATGCGGTGGTAGCAAAGGAAGCCTGGGAAAATCACAGGCTGAGGAATG ACTCTGTGATTGTGGATACTTTCCATGGCCTTTTCAAATCTACTTTGGTTTGCCCAGAATGTGCTAAAGT TTCTGTGACCTTTGACCCATTTTGCTATCTAACTCTCCCACTGCCTTTGAAGAAAGATCGAATTATGGAG GTCTTCCTGGTTCCTGCTGACCCTCATTGCAGACCTATCCAGTACCGTGTGACTGTGCCATTGATGGGGG CCATTTCTGACCTGTGTGAAGCACTCTCCAAGCTGTCTGGCATTGCTGCAGAAAATATGGTGGTCACTGA TGTGTATAATCACCGCTTCCACAAAATTTTCCAAATGGATGAAGGTTTAAGCCACATCACGCCTCGAGAT GACATTTTTGTGTATGAGATCTGCACCACTCCCATGGATGGCTCAGAATATATCACTCTCCCAGTCTACT TCAGAGAAAAGAAGTCCAGGCCATCGAGTACTTCCTCTGGGGCCGTGCTCTATGGACAGCCACTTCTTGT TTCTGTCCCTAAGCACAGACTAACCCTCGAGTCCTTATACCAGGCTGTTTGTGAACGTATCAGCCGCTAT ATAAAGCAGCCTTTGCCTGAGGAGTTTCTCAGCTCTCCCTTAGAGCCTGGGGCCTGCAATGGCTCTAGGG GTAGCTATGAAGGAGATGAAGAAGAAATGGATCATCAAGAGGAAGGGAAAGAACAGCTTTCTGAAGTGGA AGAAAGTGGTGAGGACAGTCAGGGAGGGGACCCTACTGAGACCACCCAAAAGGCGAAAGGCCCGCCCCGC CACAAGAGGCTTTTCACCTTCAGCCTCGTGAACTCCTGTGGAACTGCTGATATCAACTCGCTGGCCACTG ACGGAAAACTCCTCAAGCTCAACTCTCGATCCACACTGGCCATTGACTGGGACAGTGAGACCCGAAGCCT TTACTTTGATGAGCAAGAATCTGAGGCCTGTGAGAAGCACACGAGCATGTCCCAGCCTCAGAAGAAGAAG AAGGCTGCAATAGCCCTGCGGGAGTGCATCGAGCTCTTTACTACCATGGAGACCCTTGGGGAGCATGACC CCTGGTACTGTCCCACCTGTAAGAAGCACCAACAGGCAACAAAAAAGTTTGATTTGTGGTCTTTGCCCAA GATCCTGGTGGTTCATCTCAAACGTTTCTCCTATAACAGATACTGGCGGGATAAACTGGACACAGTTGTG GAATTCCCAGTAAGAGCTCTGAACATGTCCGAGTTTGTGTGTGACCGGGCAGCAAGGCCTTATGTCTATG ACCTGATTGCTGTGTCCAATCACTATGGCGCTATGGGGGTCGGTCACTATACTGCATATGCGAAGAACAG ACTGAATGGGAAATGGTATTACTTTGATGATAGCAGCGTGTCCCTGGCCTCTGAGGATCAGATAGTGACG AAGGCCGCCTACGTGTTGTTTTACCAGCGTCGGGATGATGAGTGTCCCAGCACCTCCTCACCTGTCAGCT TCCCGGGTTCTGATGGAGGGGCGAAGCTCAGCAGCTCACAGCAGGACTTGGGGGAAGAGGAGGCTTACAC CATGGACACCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200138 representing NM_001135012
Red=Cloning site Green=Tags(s) MAEGRGTHERPDVETQKTELGALMGTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPID NSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLEL KLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTIQDAGLYQG QVLVIEPQNEDGTWPRQTLQSKSSTAPSRNFTTSSKPSASPYSSMSASLIANGDSTNSSGMHNSGVSRGG AGFSASYNCQEPPSPHIQPGLCGLGNLGNTCFMNSALQCLSNTGPLTEYFLKDEYEAEINRDNPLGMKGE IAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFILDGLHEDLNRVKKKPYLEP KDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRIME VFLVPADPHCRPIQYRVTVPLMGAISDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLSHITPRD DIFVYEICTTPMDGSEYITLPVYFREKKSRPSSTSSGAVLYGQPLLVSVPKHRLTLESLYQAVCERISRY IKQPLPEEFLSSPLEPGACNGSRGSYEGDEEEMDHQEEGKEQLSEVEESGEDSQGGDPTETTQKAKGPPR HKRLFTFSLVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRSLYFDEQESEACEKHTSMSQPQKKK KAAIALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVV EFPVRALNMSEFVCDRAARPYVYDLIAVSNHYGAMGVGHYTAYAKNRLNGKWYYFDDSSVSLASEDQIVT KAAYVLFYQRRDDECPSTSSPVSFPGSDGGAKLSSSQQDLGEEEAYTMDTN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001135012 |
| ORF Size | 2883 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001135012.1, NP_001128484.1 |
| RefSeq Size | 4096 bp |
| RefSeq ORF | 2886 bp |
| Locus ID | 290864 |
| UniProt ID | B2GUZ1 |
| MW | 108.4 kDa |
| Gene Summary | Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200138 | Usp4 (untagged ORF) - Rat ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), transcript variant 1, (10 ug) |
CNY 12,350.00 |
|
| RR200138L3 | Lenti ORF clone of Usp4 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), transcript variant 1, (10 ug) |
CNY 14,060.00 |
|
| RR200138L4 | Lenti ORF clone of Usp4 (mGFP-tagged ORF) - Rat ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), transcript variant 1, (10 ug) |
CNY 15,490.00 |
