Vcan (NM_001170558) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cspg2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217802 representing NM_001170558 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGATAAATATGAACGGCATCCTATGGATGTGCTCAACCTTACTGTTAACGCATGCACTGCATAAAG CCAAAATGGAAGAAAACCCACCTGTTAAAGGCTCTCTGTCTGGAAAAGTGATCCTACCTTGTCATTTTTC AACCTTGCCCACCTTACCACCCGATTACAACACGAGTGAATTTCTCAGAATCAAATGGTCTAAAATAGAA GTGGACAAAAATGGAAAAGACATAAAGGAGACTACTGTCCTGGTGGCCCAAGACGGGAACATCAAGATTG GTCAGGACTACAAGGGGCGGGTATCAGTGCCTACGCATCCCGATGACGTAGGCGATGCCTCTCTCACCAT GGTCAAACTCCGTGCTAGTGACGCAGGTGTCTACCGCTGTGATGTCATGTATGGCATTGAAGACACTCAG AACACGATGTCGCTGGCCGTGGACGGTGTCGTGTTTCACTACAGGGCAGCGACCAGCAGATACACTCTGA ACTTCGAGTCTGCTCAACAGGCTTGTTTGGACATCGGGGCGGTCATAGCAACCCCAGAGCAGCTGTTCGC TGCCTATGAGGATGGATTTGAGCAGTGTGATGCAGGATGGCTGTCTGACCAAACTGTCAGATATCCCATA CGGGCTCCCCGAGAGGGCTGTTATGGAGACATGATGGGGAAGGAAGGGGTCCGGACCTATGGATTCCGCT CTCCCCAGGAAACCTATGATGTGTATTGCTATGTGGATCATCTGGACGGCGATGTGTTCCACATCACTGC TCCCAGTAAATTCACCTTCGAGGAGGCCGAAGCAGAGTGTGCAAACCGGGATGCCAGGCTGGCGACTGTT GGGGAACTTCACGCAGCTTGGAGGAACGGCTTTGACCAGTGCGATTACGGCTGGCTGTCGGATGCCAGCG TGCGGCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGTGGTCTACTTGGGGTGAGAACCCTGTATCG TTTTGAGAACCAGACATGCTTCCCTCTCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACCTAAACAG AATATATCAGAGGCAACCACCATCGAAACGAATACCCTCGCAGAAACTGCATCACCCAGTTTATCCAAAG AACCACACATGGTACCCGAGAGAGCTACACCAGTCATTCCATTAGTCACCGAATTACCTATTTTTACCAC ACACTTCCCTCCTGCAGGAAATATAGTCAATTCTGAACAAAAATCTGTAATCTACTCTCAGGCTATCACA GACAGAGTAGCCACTGAATCACCCACAGCTGCTGGGAGTCCCATTAACTCTTGGGATGCGGATGACTACT TGCCTTCTGGGTCAGGACCACTTGGAAAGCCAGACATATCTGAAATTAAGGAGGAAGGACTTCAAAGTAC AACTGTCACCTCCCAACATGCTACTGCCTCACACCATGGAATTACAGAGGATACGCAAACACATGAATCA GTTACACAGATTGAACAAATAGAAGTGGGCCCCTTGGTAACATCCATGGAAATCACAAATCACATTTCTT TCAAGGAATTTCTAGAAACCAAAACACCATTGGAATCCACAGAAGTGACCCTGGAACAGCATCAGACCGA CATGCCAACAGTTATCACCAGTCCTGAATTGGCAACCACAAGTCACTATGGAGTCACCTTGAGAGAAGAT GATAGAGAAGATATAGCACTGACAGTAAGATCTGGTCAGAGTACCCGGGTCTTTAGCCAAATACCAGAAG TCATCACGGTATCAAAGACATCAGAAGACACTACCTACTCACAGCTAGGGGACTTAGAATTCGTTTCAAC AGCAACAATAACCATGCTTGGCACAGATGGATCACTGACAGATGAAGGGAAAGAACCCCAAACTGATGAT AAGACGGCCGAAGACGAGTTTGGTCAATCACAACCTACAATCCCCTTCCCATCACAGCATCTTACAGAAG TAGAATCACTTCCATATTCTGGTGACACAATATCAGTAGAGAGAATATCCACAGTTAGTTATCCATCATT ACAAACAGATGTGACACAAGGAAGAGAGAGAACAGAAGTACCAAGGCCAGGGTTAAAAAAAGATCCTTAT GCAGTTGATGAGATACAAGAAAAGATCACCAAAGATCCATTTATAGGTACAATAGAAGAAGGCTTCTCTG GAATGTCTCTCTCTACATCTTCCTCAGAGACTTCAGCGGAAAGGACGGAATCTGTTTCCCCAGCATTGAC AATTGAGAAGTTAACTGTGAAGCCAGCCGTAGCAAGCGATGTGGACGAAATGACAATCCTGACTAGATTA GAAACAGATGTGCCAACGTCTGACGAAGATGTTACAAGTGCTCATTTGACCCATAGTACTTTAAATGTGG AAGTGGTCACAGTATTAAAATGGCCAGGGAATGAAGACAATTCAACTTCAAAGCCTTTACCCCCAACAGA ACGTGCAGGCTTTACAAAATCGCCTCCTGTCTCACTTAGCACTGTAGGAATAGTTGGAAAAGATAAAGAA ACCCCAAGTTTCACAGATGGGGGAGACGAATACACTCTTTCCCTAGATGGTACTCCCAAGCCATTAGAAA AGTTTTCTGAGGAAGATTTAACATCTGGAGAATTTGCAGTTACAATTCCTACATCTATATCAATTGACAG TGCAGAGAAGTCAACTTTGGGAGAGCCTACAACTGGAGACAGGGTTCTGTCCACCACAAGCACAAAAGAT CTGGTCATCAATGCAACTGTGGAAGGAAGTGCTTTGGATGAAGATATGGATGCCTCCAAGCCACTGTTTA CTGCCACCCCATTTGTCCACACCTCCGATGTGGAAGAATCGGCATTTGTTAATTATAGTAGCACCCAGCA GCCCACTACTTATGTAGACATATCCCACACTAGTCCTCTTTCTATAATTCCCAAGACAGAATGGAGTGTA TCGGAAACTTCTGTTCCACTGGAAGATGAGGTTCTAGGCAAATCTGATCAAGATACCCTTGAACAGACAC ATCTAGAAGCAACCATGTCTCCTGAAGCTTTGAGTACAATAGAAGTCACACAGGGAGAAACCCAGGAAGA ACCACAAACACCAGGAATCCCATTTCCTGCTCTCAGTTCTACTGCAGTTATGACCAAGGAGACAACAGCA TTTGAAGAAGAGGGGGAGGGGTCAACATATACCCTCTCTGAAGACAGATTGATGACAGATTCTGAAATAG TCCCAAGTTTAGAAACAACTCCAGTTGGTACATCTTATCCAGGTGGTGCAATGACACAGCAGGAAGTAGA AATGGATACAATGGTAACACAGATGTCAAGCATTAGGCCAACAGTAGTTTTAAGTACTGAGCCAGAAGTA AGTTATGAAGCAGAAGGTAGTAGTCCAATGGAATTTGCATCGACTTTGAAGCCCTTTGGGACTCAAGTCA CACAGCTTGTGGAAGAAACGACTGAGGAAGGGAAAAAGACACCCTTGGATTATACAGATTTAGGTTCAGG GTTATTTGAACAACCGAGAGTTACTGAGCTCCCAGATTTTTCAATGACTCCAAGTGATATCAGTGTCTTC ACTGCCATCGACAGCCTTCACAGAACCACACCCTTGAGGCCACCTTCGCCATTCACTGAGGAACCACACA TCTTTGAGAAGGAGCCCAGTGAGAAAACAACAGGTGACATAATACTTCCTAGAGAATCTGTAACACAGCA CCCTCTGACAACTCTCATGGATATCATAGCCAAGAAAACAGAATCTGATATTGATCACGAGTATCACATG ACCTCAAAGCCTCCTGTAATGCAGCCAACAAGACCATCAGTTGTGGAAAGAAAAACAACTTCCAAACCTC AAGAGTTGTCTACTTCACCACCCCCGGCAGGGACCAAATTCCACCCTGACATAAATGTTTATATTATTGA GGTCAGAGAAAACAAGACAGGTCGAATGAGTGATATGGTTGTAAATGGTCATCCAATAGATTCAGAATCT AAAGAAGAGGAACCTTGCAGTGAAGAAACAGATCCACTGCATGATCTGTTTGCTGAAATTTTACCCGAGT TACCAGATTCCTTCGAAATAGACATATATCACAGTGAGGAAGATGAAGATGGAGAGGAGGACTGTGTAAA TGCAACTGATGTAACAACCACTCCGTCAGTGCAGTACATCACTGGGAAGCCACATGTCACTACAGTGCCC AAAAACCCAGAAGCTGCAGAAGCGAGGCGTGGCCTGTATGAAAGTGTTGCGCCTTCTCAGAATTTCTCAA ATACTTCCGCAACTGACACCCATCAGTTTATACCGGCCGAAACAGAATTGTCAACTACCATGCAATTTAC CAAATCCAAAGAAGCCACTGAATTGTTAGAAATCACATGGAAACCCGAGACCTACCCTGAAACACCAGAA CACTTTTCAAGTGGTGAGCCTGATGTTTTCCCTACACTGCCATCCCATGATGGTAAAACCACCAAATGGT CAGAGTTCATCACAGAGAGCAATCCAAACACAGAAAATCCAGAACACAAACAACCTAAGCCTATACCTCT GTTTCCTGAAGAGTTTTCAGGAGAGGGTGCCATTGACCAAGCATCCCAGCAAACAATCTTTTCCAGGGCT ACAGAAGTAGCACTTGGTAAAGAGACAGACCAAAGTCCTACCATATCTACCTCTAGCATACGTTCAGGTT CTGTGTCAGTACATGCCTTGGAGGAAGATCCAATTGCTCTAACAGGAATCTCACAGACTGATGAATCCAT GTCTACTGTGGAAAGCTGGGTAGAGATGACCCCTAGCCAAACTGTAGAGTTCTCTGGGAGTTCTTCAGCT CCTACTATTGAAGGGTCTGGGGAAGTAGAAGAATATACAAATAAAATTTTCAACACGGTAACTGATTTGC CACAGAGAGAGCCTACAGATACGCTCATCCCCTTGGATATGAGCAACATCATGATCACAGACCACCACAT TTATACTCCTGCAACCACCGCTCCTTTGGATTCACAGCTACCTTCTACAGATGCACGGCCTACACAATTT GGAATTCAAACCACCACCTCTGAGTGGGTTTCCAGTACATCTTTTGAAGGAAGGAAAACAGAGGAAGACA AAGAAAGAGATACAAATGCAGCCCACACAGGGGAAGTCCAACCAGCGACAGAGAGGTCAGATCGCTTACT CTTAACTTCAGAATTAGAAAGTTCAAATGTGGCTGCATCTAGTCCTTTGGACACCTGGGAAGGTTTTGTG CCAGAGACAACATCAACAGTGTCCGAAAAGGAAATGGCAAACACAACTCCTGTTTTTACAGAAACAAGTG ATGTAGCCAATTTGGAGACACAGTCTTTTGAGCATAGTAGTAGCAGTCAGCCCAGGGTACAGGAAGAGCT GACCACTCTCTCAGGAAAGCCCCCCTTGATTTTTATGGACCTGGGTTCAGGAGATGCTAGTACTGATATG GAATTCATCACTGCTTCTTCATTTACATTAGATTTGGAGTCCGATACCAAAGTCAAAAAAGAATTACCCA GTACTCTGTCTCCCAGTGTGGAGACTTCATCCTCCTCTGAGCCAATAGGATTAGCTCCGAGTACTGTATT GGACATAGAAATTGTGGAAGTTATGAATCAGACATCCAAGAAGACACTGATTTCTGAACTATCAGGAAAG CCAACCTCCCAAGCAGAAGTAAGGGATTTATATCCAGGTCTGGGAGAAGATTTTAGTGGTGACTCCAGTG AATATCCAACAGTGTCTTCTACAACAATGAAAGAGGAAACAGTGGGGATGGGAGGCTCTGAGAATGAACG AGTTAAGGATACACAGACTTTATCATCTATACCACCTACCTCAGACAACATCAACCCAGTGCCTGACTCA AAAGGATTCGGCAGTACTGTGGCCAGCACCACAGCCTTCCCTTGGGAAGAGTTCATGACCTCAGCTGAGG GCTCAGGTGAGGAGCTGTCCTCAGTCAGGAGCTCTGTTAGTCTAGTGCTTCCACTAGGTGTGGACATTCT TCCTACTACAGAGTCTCCATATTTTGATCAAGAGTTTGAAGAAGCGGCTGCAGTGACAGAAGCAGGTAAA CAATCTGCTTTGCCAATAGCAGTGAGTGGAAATACTGTAGATCTGACAGAAAACAGGGACATAGAGGTAA ACAGCACAATGTCAGTAGACCTTCCCCAGACAATGGAGCCAGCCAAATTATGGTCTAAACCAGAAGTCAA TCCTGAAAAACAAGAGATTGGAAGCGAAACAGTGACACAGGATAAAGCTCAGGGACAAAAGTCTTTTGAA TCGCTCCACAGTTCTCTTGCACCAGAACAAACAACTTTGGAAAGCCAATCACTTATTGAAACTGAAGTCC AAACTTCATATTACTCTATGTTAACAACAATGAAAACATACAATACTAATGAGGAAGTGGAGGAGGAAGG CACGTCCATAGCTCATATGTCTACTCCAGGCCCTGGAATCAAAGGCTTGGAATCATATCCTACCCATCCT GAAGCTACTGGGAAGTCATACTCTTTCTCAGCTTCTGCCTTAGTGACTGAATCTGGACCAGCTAGAAGTG TAGTCATGGATTCCTCAACTCAAGAGGAAGAAAGTATAAAACTCTTCCAAAAGGACATGATACTAACACA TAAAGAGTCAAATTCTGATCTCTCGTTCTCTGGCCTGGGATCAGGAGAGGCTTTGCCTCCTCTCCCTACA ACATCAGTGAGTTTGACTGACATGGGAAAGATCAATAGCACATTGTATCCTGAGACATCTCACATGGAAA GCTTAGGAACAAGCATCTTAGGAGATAACCATGAAAGAATGAAAAATGTGTCAAATGAAGTTAGAACCCT TATTTCCGAAACAGGCAGCATTTCTCAAGACAGTACGGAGGCACCCAATACAACTTTGAGTGATACCAGA ACAGAAGAATCTACAACTTCACCTCTTCCTTTCATGAAGCTCATGGACACGGAACATTCTCCAAAGCAGA CCCTCAGGTGGGAAGAGGAAATCCAGACCCATAGGCCACAAACTATGACTGGACAGATGACCAATGACAA TTCTTCAGTGTCAGAAGCTGAAGCAGCAGCAACCTCTGCCCCTGCTTTCCTGCCTGAAACCTACAGTGTT GAAATGACCAAAGCCTTTGCTACATCACCGTCACAAACATCTGACTTGTTTGATGCAAATTCTGGAGAAG GATCTGGAGAAGTGGATGGTCTTGACTTAGTGTACACTTCTAGAACTACTCAGGCAAGCAGTCAAGGAGA CAGCATGTTTGCCTCTCATGGATTCATTGAAAAGCATCCAGAGGTGTCAAGGACCGAGACTGGAGCTACT GATGGATCTCCAACAGCTTCAGCGATGTTCCTGCATCAGTCAGAGTACAATGAGAGTTCCCTGTATCCAA CCAGCACACTGCCAAGCACAGTGACATATGAGAGTCCCTCAGAAGGTATTGCAGATGGTTTGCAAGACCA TATCAGATTCGAAGTTTCTACCTTGAAACCTAGCAGAAGGAAAGCCACTGAGAGCGTTATTATAGATCTA GACAAAGAGGATAGTAAGGACTTAGGGTTGGCAATTACTGAGAGTGCTATTGTTGAAATTCTCCCTGAGC TGACATCAGATAGAAATATTATTATAGACATTGATCACACTAAACCTGTATATGAGTACATCCCTGGAAT ACAAACAGACCTAGATTCAGACATACCATTGGGATCACATGGCAGTAGTGAAGAAAGCCTTGAAGTTCAA GAGAAGTATGAAGCAACTATTAACCTGTCTCCAACTGAGGAAGCATTTGACGGCTCTGGTGATGCACTTC CTGCTGGTCACACTCAAGCGATATACAATGAGTCTGTGACTCCCAGTGATGGAAAGCAGCCAGAGGACAT AAGCTTTAGTTTTGCAACTGGAATCCCAGTTTCTAGCACAGAGACAGAATTAAATACTTTCTTCCCTACA GTGTCAACCCTGCACATTCCTAGCAAACTAACCACCGCTAGTCCAGAGATTGATAAACCCAACATTGAAG CAATATCACTGGATGATATATTTGAATCAAGCACCTTGTCTGATGGCCAAGCTATTGCCGATCAGAGTGA AGTAATATCAACACTAGGCCATTTGGAAAAGACACAGGAAGAGTATGAAGAAAAGAAATATGGAGGTCCT TCTTTTCAGCCAGAATTCTTTTCAGGAGTGGGGGAAGTATTCACAGATGCCCCTGCTTATGTAAGTATTG GTAGGACATACTCTGTAGCTCAGCCTTTAACAGAATTCCCCAATGTGGTAGGACAATCTGACTCCACACA TTACACTGAAGCAACATCTGCAGTTTCCTCTGTGACAGAGCTGTCTCCTCAGACACCATCTTCTCCCTCT CCTGTCTACATAGACAGTGGAGTCTCTGAATTCACAGAGGTCCCCCACAAAAGTGCTCAGCCAGCTCCTA CTGCCGCTTCATCACAAAAGTTGATAGAGGGTTCTTTTAAGAAAGTGCGTGCTAATATTGAAGCTACCAT CAAGTCGCTGGGTGAAAATGATCACGGAACAGAGTCTCCATCCATGTCTCCTAGCCCTGCTTTAGACATT TCAGAAGACGACAGTAAACCTAAGTTACTAGAAGACCTGGAAACTTCTCCCACAAAAACCGAGACTTCCC AAGATTCCCCAAACAAAGCAAATGATCAAATTCCAGGAAAAACAGCTGGAATCCTTGCTGGTATCAAAAC AACAGAGAGTGGACCAGTCGTTACAGCTGCAGATGACATGGAGCTAGGAGATGCCACACAGCGGCCACAT TCTGCTTCTGCTCCTGCAGCTTTCAGGGTTGAGACAAGTATGGTACCTCAGCCCATCCCACAGGAGCCTG AGAGGCCAACCTTTCCTTCCCTGGAAATTAACCATGAAACACACACATCATTATTTGAAGAATCTATATT GGCAACATCTGAGAAACAAGTGTCCCAAAGAATTCTTGATTACAGTAACCAGGCAACAGTCAGTACTCTG GATCTAAATACTGAACACTCAATACCACCATTTTCCATTCTGGACAATTCTAACGAAACTGCTTTCCTGA TTGGCATTAGTGAAGAGACCGTGGAAGGCACAGCAGTTTATCTACCAGGACCTGATCTCTGCAAAACAAA CCCATGCCTCAATGGAGGCACCTGCTATCCTACTGAGACTTCCTATGTGTGCACCTGTGCACCTGGCTAC AGTGGAGACCAGTGTGAACTGGATTTTGATGAATGTCACTCTAACCCTTGTCGGAATGGAGCCACCTGTG TGGACGGTCTGAATACATTTAGATGCCTCTGCCTTCCGAGTTATGTCGGTGCACTCTGCGAACAAGACAC TGAGACATGCGACTATGGCTGGCACAAATTCCAAGGGCAATGCTACAAGTACTTTGCTCATCGCCGTACA TGGGATGCTGCTGAAAGGGAGTGTCGCCTGCAGGGTGCCCACCTCACAAGCATCCTTTCTCATGAGGAAC AAATGTTTGTGAATCGTGTGGGCCATGATTACCAGTGGATTGGCCTCAATGACAAGATGTTTGAACATGA CTTCCGCTGGACTGACGGCAGCGCACTGCAATATGAGAACTGGAGACCCAACCAGCCAGACAGCTTCTTT TCTGCTGGAGAAGACTGCGTTGTGATCATTTGGCATGAGAATGGCCAGTGGAATGACGTCCCCTGCAACT ACCACCTCACCTACACCTGCAAGAAGGGAACAGTTGCTTGCGGCCAACCCCCTGTTGTAGAAAATGCCAA GACCTTTGGAAAGATGAAACCACGTTATGAAATCAACTCCTTGATTAGATACCACTGCAAAGATGGTTTC ATTCAGCGTCACCTTCCAACTATCCGGTGCCTAGGAAATGGGAGATGGGCAATGCCTAAAATAACCTGCA TGAACCCATCTGCATACCAAAGGACTTATTCTAAGAAATACTTAAAAAATTCCTCATCAGTCAAGGACAA TTCTATAAATACGTCAAAACATGAGCATCGCTGGAGCCGGAGGTGGCAGGAAACGAGGCGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170558
Insert Size	10074 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170558.1, NP_001164029.1
RefSeq Size	12281 bp
RefSeq ORF	10074 bp
Locus ID	114122
UniProt ID	Q9ERB4
Gene Summary	extracellular matrix protein secreted by many cell types; part of the large chondroitin sulfate proteoglycan (CSPG) family [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also referred to as V0). Sequence Note: This RefSeq record was created from transcript and genomic sequence data from several strains because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

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