Frem2 (NM_001245978) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1566012
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217801 representing NM_001245978 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCCGCGGGCGCTGGACAGCCAAGTACTCCAACTTCCAGTCTATACTCACACAGTCGCCCAGAC TGCTGCTGCTGCTCCTGTCACTGGTAAGCCGCGTCTCCACACAGGATGCAGCCCCTGGCGCTGCATTTCT ATCCCTGGGTCTCTCCGGGACTTCGGGGGTCCCTACCGAGGAAGCTATAGTGGTGGCGAACCGCGGGCTC CGTGTGCCCTTCGGTCGCGAAGTCTGGCTGGATCCGTTGCGTGACTTGGTGCTGCAGGTGCAGCCAGGAG ACCGCTGCACAGTGGCCGTGCTAGACAACGACGCGCTGGCCCAGCGGCCCGGCCGCTTGAGTCCCAAGCG TTTTGCGTGCGACTACGGCCCGGGTGAGGTGCGTTACTCGCACCTGGGTGCTCGCAGCCCTTCCCGCGAC CGCGTCCGGCTGCAGCTGCGCTACGATGCTCCAGGAGGGGCCATAGTCTTGCCATTGGCACTAGAGGTGG AAGTGGTTTTCACCCAGCTGGAGGTTGTGACTCGGAATTTACCTCTGGTCGTGGAAGAACTGCTAGGGAC CAGTAATGCCCTGGATGCTCGGAGCTTAGAGTTCGCCTACCAGCCTGAGACCGAGGAGTGCCGCGTGGGC ATCTTGTCAGGTTTGAGCGCTTTGCCTCGTTATGGAGAACTCCTGCACTACCCACAGGTCCCGGGAGGGA CCGGAGAGAGAGGGTCCTCCGAGACCCCTTTGATGGACTGCAAAGCATTCCAGGAGCTGGGAGTGCGCTA TCGTCACACAGCCCTGAGTCACTCACCTAACAGGGACTGGCTGCCTATGGTGGTGGAGATACATTCAAGA GGGGCTCCAGTGGGCAGCCCGGCTTTAAAGCGTGAGCATTTCCAGGTGCTTGTGAGGATCCGGGGAGGTG CGGAGAACACTGCGCCCAAGCCCAGTTTCGTGGCCATGATGATGATGGAGGTAGACCAGTTTGTACTGAC TGCTCTCACTCCAGACATGCTGGCAGCAGAGGATGCTGAATCCAACCCGGACCTTTTGATTTTCAACCTC ACCTCTGCCTTCCAGCCTGGGCAAGGCTACTTGGTGAGCACTGATGATAGAAGCCTGCCTCTGTCCTCCT TTACACAGCGGGATTTGCGCCTGCTGAAGATTGCCTACCAGCCTCCCTCTGAAGACTCCGATCAGGAGCG CCTCTTTGAGCTAGAGCTGGAGGTAGTGGACCCTGAAGGAGCAGCCTCAGACCCTTTCGCCTTCATGGTG GTGGTGAAACCCATGAACACACTGGCTCCAGTGGTCACACGGAACACTGGCCTCATTCTCTATGAGGGTC AGTATCGGCCACTCACGGGTCCTGTAGGCAGTGGACCCCAGAACTTGGTCATCAGTGATGAAGATGACCT AGAAGCTGTGAGACTGGAGGTGGTGGCCGGGCTCCGGCATGGTCACCTTGTTATTCTGGGTTCTCCCAGT AGTGACCCTGCTCCCAAGACCTTCACTGTGGCTGAGCTGGCCGCTGGCCAGGTGGTCTACCAGCATGATG ACAGAGACGGTTCACTGTGTGACAACTTGGTGCTGCGCATGTCAGATGGAGGAGGCAGGCACCAGGTCCA GTTCCTGTTCCCTATCACTCTGGTGCCTGTGGATGACCAGCCACCTGTCCTGAACGCCAACACTGGGCTG ACAGTGGCAGAGGGTGACACCGTGCCAATCCCGCCCTCGACTCTGAGTGCAACTGATATGGACTCGGATG ACTCTCAGCTGCTTTTTGTACTGGAGCCGCCCTTCTCAAGCTCAGGACGCCTGCTTCTCCGCCAAACGCA TGCTCCCCAGGAAGAGCAGGGTCTGTGGCACAAACAAGGGTCCTTTTATGAGCGAATGGTGACAGAGTGG CGGCAACAGGATATAACGGAGGGGAAACTCTTCTACAGGCACTCGGGGCCCCACAGCCCTGGGCCAGTGA TGGACCAGTTCCTGTTTAGAGTCCAAGACAATCAAGACCCCCCTAATCAGTCTGGGATCCAGAGGTTTGT AATACGCATCCATCCCGTGGACCGTCTCCCTCCAGAACTGGGCAGTGGTTGTCCCCTTCGGATGGTGGTA CAGGAATCCCAGCTCACACCACTGAGGAAAAGGTGGCTGCACTACACTGACCTAGACACAGACGACCGAG AGCTCCACTACACAGTGACCCAGCCCCCTACGGACACAGATGAGAACCACTCACCGGCTCCGCTGGGGAC TCTGGTCTTCACTGATAATCCCTCAGTTGTGGTGACCCATTTTACCCAAGCCCAGGTCAACCATCATAAA ATTGCTTACAGGCCCCCAGGGCAGGAGCTAGGAGTAGCAGCCCGAGTGGCTCAGTTCCAGTTCCAAGTAG AGGATCGAGCTGGGAATGTGGCTCCAGGCACCTTCACCCTCTACCTGCAGCCTGTGGATAACCAGCCCCC CGAGATTGTCAACACGGGCTTCACTGTTGAGGAGAAGGGCCACCACGTCCTTCGTGAGACTGAGCTGCAT GTGAGTGACGTTGACACGGATGTGGCCCACATCTCGTTCACTCTCACACAGGCCCCCAAACATGGCCACT TGCAAATATCTGGACGGCCACTGCATGTAGGAGGACAGTTCCGTTTAGAGGACATAAAGCAGGGCAGAAT TTCCTATCTGAATAGTGGGGACGAATCTTTGACTGACAGCTGCTCCCTGGAGGTCAGCGACAGACACCAC GTGGTGCCAATCACTCTCAGAGTGAACGTTCGGCCAGCGGACCGTGAAGGTGCTATGTCAGTCCTTCCTG CTGGTACTCTGGAGTCCTATCTAGATGTTTTGGAGAATGGGGCTACCGAAGTCACTGCCAACATCATTAA GGGGACCTATCGGGATACCGATGATTTGATGTTGACTTTCCTGTTGGAAGGTCCGCCCTTATATGGGGAG ATCCTGGTCCACGGAGCTCCAGCCGAGCAGTTCACCCAGAGGGACATCCTGGAGGGCTCTGTTGTCTACG CTCATACCAGTGGTGAGATTGGCCTGTTGCCCAAAGCAGACTCTTTTAACCTGAGTCTGTCAGCTATGTC TCAAGAATGGAGAATTGGTAGCAGTGTTATCCAAGGCGTTACTGTGTGGGTGACCATCCTCCCCGTGGAC AGCCAAGCCCCTGAAATTTCTGTTGGTGAGCAGTTTGTGGTGCTGGAAGGGGACAAGAGTTTGATAAGCC TGACCCACCTAAGTGCTGAGGACGTGGACTCCCTCATGGATGACCTCTTGTGCACAATAGTCATTCAGCC AACCTCGGGCTATGTAGAGAACATTTCTCCAGCACCAGGCTCAGAAAAATCAAGGGCTGGGATTGCCATA AGTGCCTTCACGCTGAAGGATCTCAGGCAAGGACACATTAACTATGTCCAGAGTGTCCACAGAGGGGTGG AGCCTGTGGAAGACCGCTTTATATTCCGTTGTTCTGACGGCATTAACTTTTCTGAGAGACAGATTTTCCC CATTGTAATCATTCCTACCAACGATGAGCAGCCAGAAATGTTCATGAGGGAATTCATGGTGATGGAAGGC ATGAGTCTGGTGGTCGATACACCCATCCTCAACGCTGCCGATGCCGACATTCCCCGGGATGATTTAACGT TCACCATTACCCGGTTTCCGACTCATGGTCATATCATGAACCAGCTGATCAATGGTACAGTGTTGGTTGA AAGCTTCACCTTGGACCAGATTATAGAGAGTTCCAGCATTATTTATGAGCATGATGACTCTGAGACACAA GAGGACAGTTTTGTGATTAAGTTGACAGATGGCAAGCACTCTGTGGAGAAGATGGCCCTCATCATGGTCA TCCCTGTTGATGACGAGACGCCCAGAATGGCCATCAATAACGGGCTAGAAATAGAAATTGGGGAAACCAA AGTTATCAATAACAAAGTTCTAGCGGCAACTGATTTAGACTCTGATGACAAATCACTGGTTTATATTATT CGGTATGGACCGGGGCATGGCTTGTTACAGAGACGGAAGCCTCTAGGTGTCTTTGAAAATATCACGCTAG GCATGAACTTTACCCAGGATGAAGTGGACAGGAACTTAATTCAATATGTCCACTTTGGGCAAGAGGGCAT TCGAGACCTAATTAAATTCGATGTGACAGATGGGACGAATGCTCTCATAGACCGCTACTTCTACGTGTCT ATTGGAAGTGTTGACATTGTCTTCCCTGATGTGGTAAGTAAGGGCGTGTCCTTGAAAGAAGGTGGCAAGG TCACTCTGACAACCGACCTGCTAAGCACAAGCGACCTGAACAGTCCTGATGAGAACTTAGTTTTCACCAT TACCAGGGCTCCCATGAGAGGTCACCTGGAGTGCACGGACCAAAGAGGACTGTCCATCACTTCTTTCACT CAGCTCCAGCTGGCTGGCAACAAAATCTACTACATACACACAGCCGAGGATGAGGTCAAAATGGACAGTT TCGAGTTTCAAGTCACCGACGGCCGGAACCCTGTCTTCCGAACTTTCCGGATCTCAATCAGCGACGTAGA CAACAAAAAGCCAGTGGTCACCATCCACAACTTGGTGGTCAGTGAAAGTGAAAGTAAGCTTATCACCCCC TTTGAACTCACCGTGGAAGACAGAGATACCCCCGACAGGCTCCTAAAATTCATCGTCACCCAGGTCCCTG TTCATGGTCACCTCCTCTTCAACAACACCAGATCTGTCATGGTTTTCACCAAGCAAGACTTGAATGAAAA CTTAATCAGCTACAAACACGATGGCACAGAATCCACGGAAGACAGCTTCTCCTTCACGGTGACTGACGGC ACCCACTCGGACTTCTATGTTTTTCCTGATACAGTGTTTGAGACTAGGAGACCTCAAGTGATGAAAATCC GGGTGTTACCTGTTGACAACCGTGTCCCCCAAATTGTAGTGAACAAAGGAGCTTCTACACTTCGGACTCT GCCCACCGGCCATTTGGGGTTCATGATCACGAGCAAAATATTGAAAGTGCAGGACAGAGACAGCCTGCAT TTTTCTCTCAGGTTCATTGTGACAGAAGCCCCCCAACATGGCTATCTTCTCAACCTGGGCCAAGGAAACC ACAGTGTCACACAGTTTACCCAAGCTGACATTGACGACATGAAAATATGCTATGTCTTGAGAGAGAGAGC AAATGCCACGAGCGATGTGTTCCATTTCATAGTGGAAGATGACGGTGGAAACAGGTTAACCAACCAGCAT TTTCGACTGAATTGGGCATGGATCTCTTTTGAAAAGGAGTATTACCTGATCAACGAAGATTCCAAATTTC TGGATATTGTTCTTAAACGCAGAGGATACTTGGGAGAAACTTCGTTTATAAGTATCAGCACAAGGGATGG GACTGCAGAAAAAGACAAAGACTTCAAGGGTAAAGCTCAGAAACAAGTGCAGTTCAATCCGGGCCAAACC AGGGCCTCGTGGAGAGTGCGGATCCTGAGCGATGGGGAACACGAGCACTCCGAGACCTTCCAAGTGGTTC TCTCTGAGCCGGTGCTGGCAGTCCTGGAGTTCCCGGAAGTGACTACGGTGGAGATCATTGACCCAGGCGA TGAATCAACCGTGTTCATTCCCCAGTCTGAATACTCAGTTGAAGAAGATGTGAGCGAGCTGTTCATTCCC ATCAGGAGGAGTGGAGACATCAGCCAGGAGCTGATGGTGATCTGCTATACACAGCAAGGGACAGCAACCA GCACTGTGCGGACATCCGTGCTGTCTTACTCTGACTACATCTCCAGGCCTGAGGACCACAGCAGCGTGAT CCGCTTTGACAAAGATGAACGTGAGAAAATGTGTCGTGTGGTGGTGATTGATGACTCGTTGTACGAAGAG GAAGAGACTTTCCAGGTCCTTTTGAGCATGCCCATGGGCGGGAGGATCGGCAACAAGTTTCCGGGAGCCA ATGTGACCATCCTCACAGACAAGGATGATGAGCCTGCCTTCTACTTTGGGGATACCCAGCATTCGGTGGA TGAAAGTGCTGGCTACGTGGAGGTGCAGGTGTGGAGGACGGGGACCGACCTCACGAAGCCTTCTAGTGTC ACGGTGAGGTCCCGGAAAACAGAACCTCCCTCTGCAGACGCTGGAACAGATTATGTGGGAATCAGTCGCA ATTTAGACTTCGCACCTGGCGTCAGCATGCAGACCGTTCGCGTTGTCATCCTCGATGACCTGGGACGACC AGTGCTGGAGGGAATCGAGAAGTTTGAACTGGTACTTCGAATGCCTATGAACGCTGCCCTTGGCGAACCC AGCAAGGCCACCGTGTCCATAAATGACTCTGCCTCAGACTTGCCCAAAATGCAATTCAAAGAAAGAGTGT ACACTTGCAGCGAAAATGATGGACACGTGGTTGCAATGATCTACAGGAGTGGTGACCTCCAGCACAGGTC TTCTGTGAGATGCTACACGAGGCAGGGGTCTGCCCAGGTGATGATGGATTTTGAAGAACGCCCAAATACC GACATTTCCATTGTCACATTCCTCCCTGGTGAGTCGGAGAAGCCGTGTGTCCTTGAACTGATGGACGATG ATCTCTATGAGGACGTGGAGGAGCTGCGCCTGGTCCTCGGCACACCACAGGGCAGCTCTGCCTTTGGAGC TGCAGTGGGCGAGCAGAACGAAACCCTGATAAAGATCCAGGATGATGCTGACAAAGCTGTTATCAAATTT GGAGAAACCAAATTTAGTGTCACTGAACCCAGCAGACCAGGAGAGTCGGTGGTTGTCAAAATTCCGGTGA TCCGCCAAGGAGACACTTCGAAGGTTTCCATCGTGAGAGTGCACACCAAGGATGGCTCGGCTACCTCTGG AGAGGATTACCACCCTGTGTCGGAAGAAATCGAGTTTAAGGAAGGGGAAACGCAACACACTGTTGAAATT GAAGTAATCTTCGATGGGGTGAGAGAGATGAGAGAGGCTTTCACTGTTCACCTTAAACCGGATGAAAATA TGGTAGCAGAGACACAGACAACCAAGGCCATTGTATATATAGAAGAAATCAACAGCATGGCAGATGTCAC CTTTCCTTCTGTCCCTCACATTGTGTCCTTGATGGTATATGATGACACTTTCAAAGGCAGAGAGGACGCT GGACCTGTTTCTGGCTATCCTATCATCTGCATTACGGCCTGCAACCCCAAATATCCAGACTATGACAAAA CAGGCTCTATTTGTGCCAGTGAGAACATCAACGACACTCTGACCCGATACAGATGGCTGACCAGTGCTCC CACTGGCCCGGATGGTGTAGCCAGCCCTATGAGAGAGGTGGACTTCGACACCTTCTTCACATCATCCAAG ATGATCACACTAGACTCCATATACTTTCAGCCAGGCTCTCGGGTACAGTGTGCGGCCCGAGCAGTGAACA CCAATGGGAACGAAGGCCTGGAACTAATGAGCCCCATCGTCACCATTGCCAGAGAGGAAGGTCTCTGTCA GCCCCGTGTGCCTGGGGTAGTGGGGGCAGAGCCGTTCTCAGCAAAGTTGCGCTACACTGGCCCGGAGGAC CCAGACTTTGCCAACCTCATCAAGCTCACGGTCACGATGCCACACATAGACGGCATGCTCCCGGTCATCT CCACGAGAGAGCTGTCCAACTTTGAGCTGACGCTCAGCCCAGATGGCACAAGAGTTGGGAATCACAAATG CTCCAACCTGCTGGACTACAGTGAAGTGAGGACCCACCATGGCTTCCTGACTAATGCCACGAAGAACCCG GAGGTCATTGGAGAGACCTATCCTTACCAGTACAGTGCGCCGGTCAGGGGTTCCAGCACCTTACGCTTCT ACCGAAACCTGAACCTGGAGGCCTGTCTGTGGGAGTTTGTCAGCTACTACGACATGTCGGAGCTCCTGGC TGACTGTGGAGGAACCATCGGGACCGATGGCCAGGTCCTCAACCTCGTGCAGTCCTACGTGACCCTTCGA GTTCCTCTGTATGTTTCCTACGTGTTCCACTCCCCCGTGGGCGTGGGAGGCTGGCAGCATTTCGACTTGA AGTCCGAGCTCCGGCTCACATTCGTGTACGACACTGCCATCTTGTGGAACCATGGGATCGGCAGCCCCCC GGAAGCTGAACTGCAAGGCTCTCTCTATCCAACCAGCATGCGCATTGGTGAGGAGGGACGCCTGGCTGTG AACTTCAAGACAGAAGCTCAGTTCCACGGCTTGTTTGTGCTGTCCCATCCTGCTTCCTTCACCAGCTCAT TGATTGTGTCAGCTGACCATCCGGGCCTCACATTTTCCCTCCGCCTTATACGGAGTGAACCAACCTACAA TCAGCCAGTCCAGCAGTGGAGTTTCATCTCAGACTTTGCAGTTCGTGACTACTCGGGCACCTACACAGTA AAGCTGGTGCCTTGCACCACCCCATCCAACCAGGAGTACCGTTTGCCGGTCACCTGCAACCCCAGAGAGC CTGTCACCTTTGACCTTGACATTCGATTCCAACAGGTCAGCGACCCCGTGGCAGCTGAGTTCAGCCTGAA CACCCACATGTATCTGCTGTCTAAGAAGAGTCTCTGGTTGTCTGATGGGTCCATGGGATTTGGGCAGGAG AGCGATGTTGCCTTTGCAGAAGGGGACGTGATTTATGGTCGTGTCATGGTGGATCCTGTCCAGAACCTTG GTGACTCCTTTTACTGCAGCATTGAGAAGGTGTTCTTATGCACGGGGGATGATGGCTACGTGCCCAAGTA CAGCCCGGCAAATGCAGAATATGGCTGCCTGGCTGATTCTCCTTCACTCTTACATAGATTCAAAATTGTG GATAAGGCTCAGCCAGAGACACAAGCCACAAGCTTTGGAGATGTCCTGTTTAACGCCAAACTGGCGGTGG ATGACCCTGAAGCAGCTCTCTTAGTGAATCAGCCTGGATCAGATGGTTTTAAAGTAGACTCAACGCCACT CTTTCAGGTTGCCTTGGGCCGAGAATGGTACATACACACAATCTATACGGTGAGGTCAAAGGGCACCTCC CATCGAGGCATCGGCAAGAGGAGCCTGGAGTACCAGTATCACTCTGTGGTGCAGCACGGACCGCCCCAGG CAACTACCAAGAGCTGGAAGAAGAGAGCAGTCAGGAGCACGCCCTCACTCGCAGGGGAAATCGGTGCCGA GAACAATCGAGGAACAAATATCCAACACATCTCCCTGGACCGCCGTGGCAAGAGGCAGGTCCCCCATGGA AGAATTCCTCCTGACGGCATCCTGCCCTGGGAGCTCAATAGTCCCAGCTCTGAAATGAGCCTTGTCACTG TCCTGGGAGGCATCTCGGTGGGTTTGCTTGCTGTCTGCTTGGCTGTGGCCGTGGCAGTCATGTGCAGGAA CCGGAGTATCAAGGGCAAGGATGCTCCAAAGGGCTCTGGGAGCACTGAGCCCATGATGTCCCCGCAGAGC CACTACAATGACAGCTCAGAGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001245978
Insert Size	9477 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001245978.1, NP_001232907.1
RefSeq Size	9810 bp
RefSeq ORF	9477 bp
Locus ID	310418

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