Tg (NM_001270783) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Tgn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217788 representing NM_001270783 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGACCTTGGTCTTGTGGGTCTCGACTTTGTTGAGCTCAGTCTGCCTGGTAGCAGCCAACATCTTTG AGTACCAAGTGGATGCACAGCCACTCCGCCCCTGTGAGCTGCAAAGGGAGAAGGCCTTTCTGAAGCAGGA TGAATATGTTCCACAGTGCTCTGAAGATGGAAGTTTCCAGACAGTTCAGTGCCAAAATGATGGCCAATCT TGCTGGTGTGTGGATTCTGATGGCACGGAAGTACCTGGCAGCAGGCAGCTGGGAAGGCCAACAGCATGTC TGTCCTTCTGCCAGCTACACAAGCAACGAATCCTGCTGAGCAGCTACATTAACAGCACAGATGCTCTCTA CCTTCCTCAGTGCCAGGATTCAGGGAACTATGCACCAGTGCAGTGCGACCTGCAGCAAGTGCAATGCTGG TGTGTAGACACAGAGGGGATGGAGGTGTATGGCACCCGCCAGCAGGGGAGGCCAACACGGTGTCCAAGGA GCTGTGAGATAAGAAGCCGCCGTCTCCTCCATGGAGTAGGAGACAAATCGCCACCCCAGTGCGATGCAGA TGGGGAGTTTATGCCTGTCCAGTGCAAGTTTGTCAACACCACAGACATGATGATTTTTGATCTGATTCAC AACTACAACAGGTTTCCAGATGCATTTGTGACCTTCAGTGCCTTCCGGAACAGGTTCCCTGAAGTGTCTG GGTACTGTTACTGTGCTGACAGCCAGGGCCGAGAGCTGGCTGAGACAGGTCCCACCAAGTGTGAAGTGGA ACAGTTTACTGCCACCAGCTTTGGTCATCCATATATCCCAAGCTGCCACAGAGATGGGCACTACCAAACA GTGCAGTGCCAGATGGAAAGGATGTGCTGGTGTGTGGATGCTCAGGGAATAGAGATCCCTGGCACTCGGC AGCAAGGGCAGCCATTGTTTTGCGCTAAAGATCAATCGTGTGCCTCAGAAAGGCAGCAGGCTTTGTCTAG GCTCTACTTTGAAACCCCAGGCTACTTCAGTCCTCAGGACCTGTTGTCTTCTGAGGACAGGCTGGTCCCT GTGAGTGGTGCCAGATTGGATATATCCTGCCCACCCAGAATCAAGGAACTCTTTGTCGACTCTGGACTCC TACGTTCTATTGCTGTGGAGCGCTACCAACAACTCTCTGAATCCAGAAGTCTACTTAGAGAAGCCATCAG AGCAATTTTCCCCTCCCGAGAGCTGGCAGGGCTTGCCCTTCAGTTCACCACCAACCCCAAGCGACTCCAG CAAAACCTCTTTGGAGGGACATTTTTGGTGAATGCTGCCCAGCTAAACTTGTCAGGAGCCCTTGGTACAA GAAGCACATTTAACTTCAGTCAGTTTTTCCAGCAGTTTGGACTCCCAGGCTTCCTGGTTAGAGACAGAGC TACTGATCTAGCCAAACTACTCCCTGTGAGTTTGGACTCCAGTCCTACCCCAGTACCTCTCCGTGTGCCT GAGAAGAGAGTTGCTATGAATAAGTCAGTTGTGGGTACCTTTGGCTTCAAAGTCAACCTACAAGAGAACC AAGATGCCTTAAAATTCCTTGTTTCTCTCATGGAACTTCCAGAATTTCTTGTCTTCTTGCAGCGTGCTGT TTCTGTACCTGAAGATAGAGCAAGAGATTTAGGAGATGTGATGGAAATGGTGTTCAGTGCTCAGGCCTGT AAACAGACATCTGGGAGGTTCTTTGTCCCATCATGCACTGCTGAAGGAAGCTATGAAGATATCCAGTGCT ATGCTGGAGAATGCTGGTGTGTGAATTCCCAAGGCAAAGAAGTTGAAGGCTCAAGAGTCAGTGGGGGACA CCCAAGATGTCCCACCAAGTGTGAGAAGCAAAGAGCTCAGATGCAAAACCTTGCAGGCGCTCAGCCTGCT GGCTCCAGTTTTTTTGTTCCTACGTGTACTAGTGAGGGATATTTTCTGCCCGTCCAGTGCTTCAACTCAG AATGTTACTGTGTTGATGCTGAAGGCCAGGTTATTCCTGGAACTCAAAGTACAATTGGTGAACCCAAGCT GTGTCCTAGTGTCTGCCAGTTACAGGCTGAACAAGCTTTCCTTGGGGTAGTAGGGGTTCTGCTCTCCAAC TCCAGTATGGTGCCTCCTATCTCCAGTGTCTACATTCCCCAATGCAGTACCAGTGGCCAGTGGATGCCTG TGCAGTGTGATGGACCCCATGAGCAGGTCTTTGAGTGGTATGAACGATGGAACACTCAGAACAGTGATGG CCAGGAACTGACTACAGCCACACTGCTGATGAAGCTCATGAGCTACAGAGAAGTAGCTTCCACAAACTTC AGCCTTTTTCTTCAAAGTCTCTATGATGCTGGCCAGCAAAGCATCTTCCCAGTGCTAGCACAGTACCCAT CCCTTCAGGATGTTCCACAGGTAGTGTTGGAGGGAGCCACAATTCAGCCTGGAGAGAACATTTTCCTGGA TCCATACATCTTTTGGCAGATCCTAAATGGCCAGCTCAGCCAATACCCAGGACCCTACTCAGACTTTAGC ATGCCTTTGGAACACTTCAACCTCAGGAGCTGCTGGTGTGTGGATGAGGCTGGTCAGGAATTGGATGGAA CTCGGACTAGGGCTGGCGAGATACCAGCATGCCCTGGCCCCTGTGAGGAAGTGAAATTTCGTGTCCTGAA ATTTATCAAGGAAACAGAAGAGATCGTCTCTGCTTCCAATGCTTCTTCATTTCCACTTGGAGAGAGTTTC TTAGTAGCCAAGGGAATCCAGCTGACCAGTGAGGAGCTTGGCCTTCCTCCACTCTACCCATCTCGTGAGG CTTTCTCTGAGAAGTTTCTACGTGGGAGTGAATATGCCATTCGTTTGGCTGCTCAGTCCACCTTGACCTT CTATCAGAAACTCCGTGCTTCCCTGGGAGAGTCCAATGGAACAGCCAGTCTTCTATGGTCAGGACCCTAT ATGCCACAATGCAATACTATTGGAGGCTGGGAGCCTGTGCAGTGCCATCCTGGGACAGGACAGTGTTGGT GTGTGGATGGGTGGGGAGAGCTCATCCCAGGCTCACTGATGGCACGCTCCTCCCAGATGCCCCAGTGTCC CACAAGCTGTGAGCTATCCCGAGCCAATGGGTTGATTTCTGCATGGAAACAGGCTGGACACCAAAGAAAC CCAGGTCCAGGAGATCTGTTTACACCAGTCTGCCTACAGACAGGAGAGTATGTCAGACAACAGACATCAG GAACAGGAGCTTGGTGTGTGGATCCATCTTCAGGAGAAGGGGTGCCAACAAACACAAACAGCAGTGCTCA GTGTCCTGGCCTCTGTGATGCCCTCAAGAGTAGGGTCCTGTCCAGGAAAGTCGGTCTTGGCTACACTCCA GTCTGTGAGGCATTGGATGGGGGCTTTTCCCCAGTGCAGTGTGACTTGGCCCAGGGCAGTTGCTGGTGTG TCCTGGCAAGTGGAGAGGAAGTGCCTGGGACTCGTGTGGTAGGCACCCAGCCTGCCTGTGAAAGCCCACA GTGTCCACTGCCATTCTCTGGGTCAGATGTGACTGATGGAGTGGTCTTCTGTGAGACAGCCTCTAGCTCA GGAGTGACCACTGTTCAACAGTGCCAGCTGTTCTGCCGCCAGGGCCTCTGGAACGTCTTTTCACCAGGGC CACTGATTTGTAACCTGGAGAGTCAGCGCTGGGTGACACTGCCACTACCCAGAGCCTGTCAGAGGCCTCA GCTGTGGCAGACCATGCAAACTCAAGCACACTTCCAGCTCCTGCTCCCACCAGGCAAGATGTGCAGTATT GACTACTCTGGCTTGCTGCAGGCCTTCCAGGTCTTCATACTGGATGAGCTGATAACCCGTGGCTTCTGCC AGATCCAGGTAAAGACATTTGGGACCCTGGTTTCCAGGACTGTCTGTGACAACTCCTCAATACAGGTGGG GTGTCTGACTGCAGAGCGTTTAGGAGTAAATGCTACATGGAAGTTACAGCTTGAGGACATCTCAGTGGGC TCACTTCCAAATTTGCACAGCATTGAAAGGGCATTAATGGGCCAGGATCTCCTTGGGCGCTTTGCAAATC TGATACAAAGTGGCAAGTTCCAGCTTCATCTGGACTCTAAGACATTCTCAGCTGATACCATCTTGTACTT TCTCAATGGGGACAGATTTGTCACCTCTCCCATGACTCAGCTTGGATGCTTGGAAGGATTTTACAGAGTT TCAACCACCAGTCAGGACCCTCTGGGCTGTGTGAAATGCCCTGAAGGAAGCTTCTCTCAGGATGGAAAAT GCACTCCCTGTCCTGCTGGCACCTACCAAGGGCAGGCAGGCAGCTCAGCTTGCATCCCATGCCCAAGGGG CAGAACAACCACCATCACTGGAGCTTTCAGCAAGACCCATTGTGTCACTGACTGTCAGAGGGATGAGGCC GGCCTACAGTGTGACCAGAATGGTCAGTACCAAGCCAACCAGAAGGACATGGACAGTGGAGAAGTTTTCT GCGTGGATAGCGAGGGGCAGAGACTACAGTGGTTACAGACAGAGGCTGGACTCTCAGAATCCCAGTGTCT GATGATGCGGAAATTTGAGAAAGCTCCAGAATCCAAGGTTATCTTTGATGCCAGTTCTCCTGTGATAGTC AAGTCCAGAGTCCCCAGTGCCAACTCCCCACTGGTGCAGTGCTTAGCAGATTGTGCAGATGATGAAGCCT GCAGCTTCGTCACAGTGTCCTCAATGTCATCAGAGGTTTCATGTGATCTTTATTCTTGGACAAGAGACAA CTTTGCCTGTGTGACTTCTGATCAGGAAGAAGATGCTGTGGACAGCTTGAAGGAAACCAGCTTTGGAAGC CTTAGGTGCCAGGTGAAAGTGAGGAATAGTGGCAAGGATTCTCTGGCTGTGTATGTGAAGAAAGGCCATG AATTTACTGCATCTGGTCAGAAGAGCTTCGAACCAACAGGTTTTCAAAATGTGCTCTCGGGGTTGTATAG CTCTGTGGTGTTTTCAGCCTTGGGAACCAATCTGACAGATACACACCTCTTCTGTCTGCTTGCCTGTGAC CAGGATTCCTGCTGCGATGGCTTCATCGTCACACAGGTCAAAGAAGGGCCTACCATCTGTGGACTTCTCA GTGCTCCTGACATTCTGGTTTGCCACATCAATGACTGGAGGGATGCCTCTGATACTCAGGCCAATGGTAC ATGTGCTGGGGTGACGTATGACCAAGGGAGCCGCCAGATGACCATGAGTCTGGGAGGCCAAGAATTCTTG CAGGGTCTGACGCTCTTGGAGGGAACTCAAGACAGCTTTATCAGCTTCCAGCAGGTCTACCTCTGGAAAG ATTCTGACATAGGGTCTCGGCCTGAATCTATGGGATGTGGAAGGGGAATGGTGCCCAAGTCAGAAGCTCC AGAAGGAGCAGATATGGCAACAGAACTTTTCTCCCCTGTGGACATTACACAGGTCATTGTCAATACAAGC CACTCATTGCCCAGCCAGCAGTACTGGCTCTCCACACACCTGTTTTCAGCAGAGCAAGCAAACCTGTGGT GCCTTTCTCGGTGTGCCCAGGAGCCTGTTTTCTGTCAACTTGCAGACATAATGGAAAGCTCATCTTTGTA TTTCACATGCTCCCTATACCCAGAAGCTCAGGTCTGTGACAATGATGTGGAATCTAATGCCAAGAACTGC AGTCAGATTCTGCCCCGCCAACCAACAGCCCTCTTCCAGAGGAAAGTTGTGCTGAATGACCGAGTGAAGA ACTTTTATACTCGCCTGCCATTCCAAAAGCTCTCAGGGATTTCCATCAGAGACAGAATCCCCATGTCTGA AAAACTCATTTCCAATGGGTTCTTTGAGTGTGAACGCCTCTGTGACAGAGACCCATGCTGCACTGGCTTT GGTTTTCTAAATGTCTCCCAGATGCAAGGAGGAGAGATGACCTGCTTGACTCTGAACAGCATGGGGATTC AAACATGCAGTGAGGAGAATGGAGCAACCTGGCGTATTTTGGACTGTGGCTCTGAAGACACTGAAGTCCA CACCTATCCCTTTGGATGGTACCAAAAGCCTGCTGTGTGGAGTGATGCTCCCAGCTTCTGCCCATCTGCT GCCCTGCAATCACTCACTGAGGAGAAGGTTGCTTTGGACTCCTGGCAGACTCTGGCCCTATCTTCAGTGA TTATAGATCCATCCATCAAGCACTTTGATGTTGCCCACATCAGCATTTCAGCCACCAGGAACTTCTCCCT GGCCCAAGACTTCTGCTTACAGGAATGTTCCCGTCATCAGGACTGCCTTGTCACTACCCTGCAGATCCAG CAAGGGGTTGTGAGGTGTGTGTTCTACCCTGATATACAGAGCTGCGAACACAGTCTACGGAGCAAGACCT GCTGGCTTCTGCTCCATGAAGAGGCTGCCTACATCTACCGGAAATCTGGAGCCCCTTTGCACCAATCTGA TGGAATATCCACACCTTCTGTACACATTGATTCCTTTGGCCAGCTTCAGGGTGGGTCCCAGGTCGTCAAG GTGGGCACTGCCTGGAAACAAGTATATCAATTCCTTGGAGTTCCATATGCTGCCCCACCCTTGGCAGAAA ACCGTTTCCAGGCACCAGAGGTCTTGAATTGGACAGGCTCCTGGGATGCCACCAAGCTAAGGTCCAGCTG CTGGCAGCCAGGTACCCGTACCCCAACACCTCCTCAAATCAGTGAAGACTGCTTATATCTCAACGTGTTT GTCCCTGAGAACCTGGTCTCCAATGCATCAGTTCTGGTGTTCTTCCACAATACTGTGGAGATGGAAGGCA GTGGAGGTCAGCTGAACATTGATGGCTCCATTCTGGCTGCAGTTGGCAACCTCATCGTAGTCACTGCTAA CTACAGACTGGGGGTCTTTGGCTTCTTGAGCTCAGGCTCTGATGAAGTAGCTGGCAACTGGGGGCTACTG GACCAAGTGGCAGCTCTAACATGGGTGCAGACCCACATTGGAGCATTTGGTGGGGACCCTCAACGTGTGA CACTGGCAGCAGACCGTGGTGGGGCTGATGTGGCCAGCATACACCTTCTCATCACCAGACCTACCAGACT CCAGCTCTTCAGGAAGGCTCTGCTTATGGGAGGCTCAGCATTGTCACCGGCTGCAATTATCAGCCCAGAT AGGGCCCAACAGCAAGCTGCAGCCTTGGCAAAGGAGGTTGGATGCCCTAACTCATCCGTCCAGGAAGTGG TCTCCTGCTTCCGCCAGAAGCCTGCCAACATTCTCAATGAAGCTCAGACCAAGCTCCTGGCTGTGAGTGG CCCTTTCCACTACTGGGGTCCTGTGGTCGATGGCCAGTACCTACGTGAACTTCCTTCCAGACGGTTGAAG AGGCCTCTGCCAGTGAAGGTGGATCTGCTCATAGGAGGTTCTCAGGACGATGGGCTTATCAACAGGGCAA AGGCTGTGAAGCAATTTGAAGAAAGCCAAGGCCGAACCAACAGTAAAACAGCCTTTTACCAGGCACTGCA GAATTCACTAGGCGGTGAGGACTCAGACGCCCGCATCCTTGCTGCTGCCATATGGTATTACTCCCTGGAG CACTCCACAGATGACTATGCCTCCTTCTCTCGGGCACTGGAGAATGCCACCCGGGACTACTTTATCATCT GTCCCATTGTCAACATGGCCAGCCTCTGGGCAAGGAGAACCCGTGGAAATGTCTTCATGTACCATGTCCC CGAAAGCTATGGCCATGGCAGCCTAGAATTGCTGGCAGATGTTCAGTATGCTTTTGGACTGCCCTTCTAC TCTGCCTACCAGGGCTACTTCTCAATGGAGGAGCAGAGCCTCTCTCTGAAAGTCATGCAGTATTTCTCCA ACTTCATCAGATCAGGAAATCCTAACTACCCACATGAGTTCTCACAGAAAGCAGCTGAATTTGCCACACC CTGGCCAGACTTTGTTCCTGGAGCTGGAGGAGAGAGCTACAAGGAGCTCAGTGCCCAGCTTCCCAATCGG CAGGGCCTCAAAAAAGCTGACTGTTCCTTCTGGTCCAAGTACATCCAGACTCTGAAGGATGCAGATGGAG CCAAGGATGGACAGTTAACCAAGAGTGGAGAGGAGGACTTGGAAGTGGGGCCTGGGTCAGAAGAAGACTT CTCAGGCTCACTGGAGCCTGTCCCCAAGAGCTACAGCAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270783
Insert Size	8163 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270783.1, NP_001257712.1
RefSeq Size	8317 bp
RefSeq ORF	8163 bp
Locus ID	24826
Gene Summary	precursor to thyroid hormone; required for thyroid hormone generation [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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