Cacna1b (NM_001195199) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	BIII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217766 representing NM_001195199 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGCTTCGGGGACGAGCTAGGCGGCCGCTATGGGGGCACCGGCGGCGGGGAGCGGGCTCGGGGCG GCGGGGCCGGCGGGGCCGGTGGCCCGGGCCAGGGGGGTCTGCCGCCGGGCCAGCGGGTCCTGTACAAGCA GTCCATTGCGCAACGCGCACGGACCATGGCCCTGTACAACCCCATCCCAGTCAAGCAGAACTGCTTCACC GTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATATGCTAAGCGCATCACCGAAT GGCCGCCCTTCGAATATATGATCCTGGCCACCATCATCGCCAACTGTATTGTCCTGGCCCTGGAGCAGCA CCTCCCTGATGGGGACAAGACTCCCATGTCTGAACGACTGGATGACACGGAACCTTACTTCATCGGCATC TTTTGCTTCGAGGCGGGCATCAAGATCATAGCTCTGGGCTTCGTGTTCCACAAAGGCTCCTACCTCCGGA ATGGCTGGAACGTCATGGACTTCGTGGTGGTCCTCACAGGGATTCTTGCCACAGCTGGAACTGACTTTGA TCTGCGCACCCTGAGGGCTGTGCGTGTGCTTAGGCCCCTGAAGTTGGTGTCTGGAATTCCAAGCTTGCAG GTGGTGCTCAAGTCCATCATGAAGGCCATGGTCCCGCTGCTGCAGATCGGGCTGCTGCTCTTCTTCGCCA TCCTCATGTTCGCTATCATCGGCCTCGAGTTCTATATGGGCAAATTCCATAAGGCCTGCTTCCCCAACAG CACAGATGCAGAGCCTGTGGGTGACTTTCCTTGTGGCAAGGAGGCCCCTGCTCGTCTGTGTGACAGTGAC ACCGAATGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAATTTTGACAACATCCTGTTTGCCA TCTTGACCGTGTTCCAGTGTATCACCATGGAGGGCTGGACTGACATCCTCTACAATACAAATGATGCGGC CGGCAACACGTGGAACTGGTTGTACTTCATCCCCCTCATCATCATTGGCTCCTTCTTCATGCTCAACCTG GTGCTCGGTGTGCTTTCAGGAGAGTTTGCCAAAGAGCGGGAGCGAGTCGAGAACCGCCGTGCCTTCCTGA AGCTCCGCAGGCAGCAGCAGATTGAGCGAGAACTGAATGGGTACTTGGAGTGGATCTTCAAGGCGGAGGA AGTCATGTTGGCAGAGGAGGACAAGAACGCAGAAGAGAAGTCCCCTTTGGATGCAGTGTTGAAGAGAGCT GCTACCAAGAAGAGCCGAAATGACCTCATCCATGCAGAAGAGGGGGAGGACCGGTTTGTAGACCTCTGTG CTGCTGGGTCTCCCTTTGCTCGTGCCAGCCTCAAGAGTGGGAAGACAGAGAGCTCATCGTACTTCCGGAG GAAGGAGAAGATGTTCCGGTTCCTTATCCGTCGTATGGTGAAAGCACAGAGCTTCTACTGGGTGGTACTG TGCGTGGTGGCCCTGAACACGTTGTGTGTGGCCATGGTACACTATAATCAGCCTCAGCGGCTTACCACTG CACTGTACTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTACGGTCT AGGGCCCAGAAGCTACTTCCGGTCTTCCTTCAACTGCTTTGACTTTGGGGTGATTGTGGGGAGTATCTTT GAAGTAGTCTGGGCTGCCATCAAGCCAGGAACCTCCTTCGGAATCAGTGTGCTGCGGGCTCTCCGACTGC TGAGGATTTTCAAAGTCACCAAGTATTGGAACTCCCTGAGGAACCTGGTTGTTTCCCTCCTCAACTCCAT GAAGTCCATCATCAGCCTTCTCTTCCTGCTTTTCCTTTTCATTGTGGTCTTCGCTCTGTTGGGGATGCAG CTGTTTGGGGGACAGTTCAACTTTCAAGATGAGACTCCAACCACCAATTTTGATACCTTCCCAGCTGCCA TCCTCACTGTGTTTCAGATTCTGACAGGAGAGGACTGGAATGCAGTCATGTATCATGGGATTGAGTCACA AGGAGGAGTCAGCAAAGGCATGTTTTCATCCTTTTACTTCATCGTCCTGACACTGTTTGGAAACTACACC CTGTTGAACGTTTTCTTGGCCATTGCTGTGGACAACCTTGCCAATGCCCAGGAGTTGACCAAGGATGAAG AGGAGATGGAAGAGGCAGCCAATCAGAAGCTTGCTCTTCAGAAGGCCAAAGAAGTAGCTGAAGTCAGCCC CATGTCTGCTGCCAACATCTCCATTGCTGCTTTTGTAAAGCAAACTCGAGGTACTGTATCTCGCAGCTCA TCTGTCTCCAGCGTAAACTCACCGCAGCAGAACTCGGCCAAGGCGCGCTCAGTATGGGAGCAGCGGGCCA GTCAGCTAAGGCTCCAGAACCTGCGTGCCAGCTGTGAGGCACTGTACAGTGAGATGGACCCGGAGGAGCG CCTGCGTTATGCCAGCACGCGCCACGTGAGGCCAGACATGAAGACACACATGGACCGACCCCTAGTGGTG GAACCTGGTCGGGATGGCCTGCGGGGACCCGCCGGGAACAAGTCAAAGCCTGAGGGCACGGAGGCCACCG AAGGTGCGGATCCACCACGCCGACACCACCGGCATCGTGATAGGGACAAGACCTCAGCCTCAACCCCTGC TGGAGGCGAACAGGACAGGACAGACTGCCCAAAGGCCGAAAGCACCGAGACCGGGGCCCGGGAGGAACGT GCGCGCCCTCGTCGAAGTCACAGCAAGGAGGCTCCAGGGGCTGACACACAAGTGCGTTGTGAGCGCAGTA GACGTCACCACCGGCGCGGATCCCCGGAGGAGGCCACTGAACGGGAACCTCGGCGCCACCGTGCCCACCG GCACGCACAGGACTCAAGCAAGGAAGGCAAGGAGGGCACTGCACCGGTGCTTGTACCCAAGGGCGAGCGT CGCGCAAGACATCGAGGCCCGCGTACGGGCCCCCGTGAGACAGAGAACAGTGAGGAGCCCACACGCAGGC ACCGTGCAAAGCATAAGGTGCCACCAACACTTGAGCCCCCAGAGAGGGAGGTTGCAGAGAAGGAGAGCAA CGTGGTGGAAGGGGATAAGGAAACTCGAAATCACCAGCCCAAGGAACCTCGCTGTGACCTGGAGGCCATT GCGGTTACAGGCGTGGGCTCTCTGCACATGCTGCCCAGCACCTGTCTCCAGAAAGTGGACGAACAGCCAG AGGATGCAGACAACCAGCGTAATGTCACCCGGATGGGCAGTCAGCCCTCAGACCCCAGCACCACTGTGCA TGTCCCAGTGACACTGACAGGCCCTCCCGGGGAGGCCACTGTAGTTCCCAGTGCTAACACGGACCTGGAA GGCCAAGCGGAGGGCAAGAAGGAGGCAGAGGCTGACGATGTGCTGAGAAGAGGCCCCAGGCCCATCGTTC CCTACAGTTCCATGTTCTGCCTCAGCCCCACCAACCTACTCCGTCGCTTCTGCCATTACATTGTGACCAT GCGGTACTTTGAGATGGTGATTCTTGTGGTCATCGCCTTGAGCAGCATTGCCCTGGCTGCTGAGGATCCC GTGCGGACCGACTCATTCCGGAACAATGCTCTGAAGTACATGGACTACATCTTTACAGGAGTCTTCACCT TTGAGATGGTCATAAAGATGATAGACTTGGGCCTGCTGCTGCACCCTGGGGCCTACTTCCGGGACCTGTG GAACATTCTGGACTTCATTGTTGTCAGTGGAGCCCTGGTGGCATTTGCATTCTCAGGATCCAAAGGGAAA GACATCAATACCATCAAGTCTCTGAGAGTCCTGCGAGTCCTGCGGCCCCTCAAGACCATCAAGCGGCTGC CTAAACTCAAGGCTGTGTTTGACTGTGTGGTGAACTCTCTGAAGAATGTCTTGAACATCCTGATCGTCTA CATGCTCTTCATGTTTATATTTGCCGTCATCGCCGTCCAACTCTTCAAAGGGAAGTTCTTTTACTGCACT GATGAGTCCAAGGAGCTGGAGCGGGACTGCAGGGGTCAGTATTTGGATTATGAGAAGGAAGAGGTAGAAG CCCAGCCAAGGCAGTGGAAGAAATATGACTTCCACTATGACAATGTGCTCTGGGCCTTGCTGACTCTGTT TACGGTGTCCACAGGAGAGGGGTGGCCCATGGTGCTGAAACACTCTGTGGACGCCACCTATGAGGAGCAG GGGCCAAGCCCCGGGTTTCGGATGGAGCTTTCCATCTTCTATGTGGTCTACTTTGTGGTCTTCCCTTTTT TCTTTGTCAACATCTTTGTGGCCTTGATCATCATCACCTTCCAGGAGCAGGGGGACAAGGTGATGTCTGA GTGCAGTCTGGAAAAGAATGAGAGGGCTTGCATTGACTTTGCCATCAGCGCCAAACCCCTGACACGGTAC ATGCCTCAGAACAAGCAGTCGTTCCAGTATAAGACATGGACATTTGTGGTCTCTCCACCCTTTGAGTACT TCATTATGGCCATGATAGCCCTCAACACAGTGGTGCTGATGATGAAGTTCTACGATGCCCCTTATGAGTA CGAGCTGATGCTGAAGTGCTTGAACATCGTCTTCACATCCATGTTCTCTCTGGAGTGCATCCTGAAGATC ATCGCCTTCGGGGTGTTGAACTACTTCAGAGATGCCTGGAACGTCTTTGACTTTGTCACTGTTTTGGGAA GTATTACTGATATTTTAGTAACGGAGATTGCGGAAACGAACAACTTCATCAACTTGAGCTTCCTTCGCCT CTTCCGGGCAGCACGGCTGATCAAGCTGCTTCGCCAGGGCTACACCATCCGCATCTTGTTATGGACCTTT GTCCAGTCCTTTAAGGCGCTGCCCTACGTGTGCCTCCTCATTGCCATGCTGTTCTTCATCTACGCCATCA TCGGCATGCAGGTTTTTGGAAACATTGCCCTTGATGATGGCACCAGCATCAACCGACACAACAACTTCCG GACATTTCTGCAAGCCTTAATGCTGTTGTTCAGGAGTGCCACTGGGGAGGCCTGGCACGAAATCATGCTG TCTTGCCTGGGCAACCGGGCCTGCGACCCACATGCCAACGCCAGCGAATGCGGGAGCGACTTTGCCTATT TTTATTTTGTCTCCTTCATCTTCCTCTGTTCCTTTCTGATGCTGAACCTCTTTGTTGCTGTGATCATGGA CAATTTCGAATACCTCACGCGGGATTCTTCCATCCTAGGGCCGCACCACCTCGATGAATTCATTCGCGTC TGGGCTGAATACGACCCAGCTGCGTGTGGGCGCATCAGTTACAATGACATGTTTGAGATGCTGAAACACA TGTCCCCACCTCTGGGTTTGGGGAAGAAATGCCCGGCTCGAGTTGCATACAAGCGCCTGGTTCGAATGAA CATGCCCATATCCAATGAGGACATGACGGTACACTTTACATCCACACTGATGGCCCTCATCCGGACGGCA CTGGAGATCAAGCTTGCCCCAGCGGGGACAAAACAGCACCAATGTGATGCTGAGCTGAGGAAGGAGATCT CTTCTGTGTGGGCTAATCTGCCCCAGAAGACTCTGGACTTACTGGTGCCACCCCACAAACCTGACGAGAT GACAGTGGGGAAGGTCTATGCGGCTCTCATGATATTTGACTTCTACAAACAGAACAAAACCACCAGAGAT CAGACTCACCAAGCTCCTGGAGGCCTGTCCCAGATGGGTCCTGTTTCCCTGTTCCATCCTCTGAAGGCCA CCCTGGAGCAGACACAGCCCGCTGTGCTCCGAGGAGCTCGGGTTTTCCTTCGACAAAAGAGTGCAACTTC CCTCAGCAATGGGGGCGCCATACAAACCCAGGAAAGTGGCATCAAGGAGTCCCTGTCCTGGGGCACGCAG AGGACCCAGGACGTACTTTATGAGGCCAGAGCACCTCTAGAACGTGGCCATTCTGCAGAGATCCCTGTGG GGCAGCCAGGAGCACTGGCTGTAGATGTCCAGATGCAGAACATGACATTGAGAGGACCGGATGGGGAGCC CCAGCCTGGCCTGGAGAGCCAAGGCCGAGCGGCCTCTATGCCACGCCTGGCGGCAGAAACACAGCCGGCC CCTAATGCCAGCCCCATGAAGCGCTCCATCTCCACACTGGCTCCACGCCCGCATGGGACTCAGCTTTGCA ACACAGTCCTGGACCGGCCACCTCCTAGCCAGGTGTCCCATCACCACCACCACCGCTGCCACCGGCGCAG GGACAAGAAGCAGAGGTCCCTGGAAAAGGGGCCCAGCCTGTCTGTTGACACAGAAGGTGCACCAAGTACT GCTGCAGGATCTGGCCTGCCCCATGGAGAAGGGTCCACAGGCTGCCGGCGGGAGCGTAAGCAAGAGCGAG GCCGGTCCCAGGAGCGGAGGCAGCCCTCCTCCTCTTCTTCAGAGAAGCAGCGCTTCTATTCCTGTGACCG CTTTGGGAGCCGGGAGCCCCCACAACCTAAGCCCTCCCTCAGTAGCCACCCCATATCGCCAACAGCGGCA CTAGAGCCAGGACCCCACCCGCAGGGCAGTGGTTCCGTTAATGGGAGCCCCTTGATGTCAACATCTGGTG CTAGCACGCCGGGCCGAGGTGGGCGGAGGCAGCTCCCCCAGACTCCCCTGACCCCACGCCCCAGCATCAC CTACAAGACGGCCAATTCCTCGCCTGTCCACTTTGCTGAGGGTCAGAGTGGCCTTCCAGCCTTCTCCCCT GGCCGTCTCAGCCGCGGCCTTTCTGAACACAATGCCCTGCTCCAGAAAGAGCCCCTGAGCCAGCCTCTAG CTTCTGGCTCCCGCATTGGCTCTGACCCTTACCTAGGGCAGCGTCTGGACAGTGAGGCCTCTGCCCACAA CCTGCCTGAGGATACACTCACCTTTGAAGAGGCCGTGGCCACCAACTCTGGCCGCTCCTCCAGGACTTCC TATGTGTCCTCCCTCACTTCCCAATCCCACCCTCTCCGCCGTGTACCCAATGGCTACCACTGCACTTTGG GACTCAGCACCGGCGTCCGGGCGCGGCACAGCTACCACCACCCAGACCAGGATCACTGGTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001195199
Insert Size	7065 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001195199.1, NP_001182128.1
RefSeq Size	7065 bp
RefSeq ORF	7065 bp
Locus ID	257648

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.