Ascc3 (NM_001277057) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Helic1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217751 representing NM_001277057 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTACCTCGCCTCACAGGAGCCTTGCGCTCCTTTTCAAATGTCACCAAGCAAGATAATTATAATG AAGAAGTAGCTGACTTAAAGCTGAAGCGATCCAAACTTCATGAACAAGCTTTAGATTTCGGCCTTTCATG GAAGAAGATAGTGAAGTTTTTGAATGAAAAACTAGAAAAGAATAAAATGCAAACTATAAATGAAGACTTA AAAGATATACTACAAGCTGCAAAACAAATCGTTGGAACGGATAATGGGAATGAAGCAATTGAAAGTGGGG CTGCATTCCTCTTTATGACGTTTCACATGACAGACTCTGTTGGTTACACGGAGACAAAGGCTATCAGACA GATGTTTGGACCCTTTCCATCATCCTCTGCTACCTCAGCTTGTAATGCCACTAGTCGGATTATTTCGCAT TTTAGTCAAGATGATCTCACTGCTTTAGTCCAGGCGACAGAAAACCAATGTAATGATAGAGTGGTCTTTG GTAGAAACTTAGCATTTTCCTTTGACATGCATGATCTGGACCACTTTGATGAATTACCAGTAAATGGTGA AGCCCAGAAAACTATAAGCTTAGACTACAAGAAGTTCCTGAATGAACAGTTTCAGGAACCCTACACCCCA GAACTCAAGCCCGTGGAAAAATCAAATGGCTCCTTATTGTGGTGTGAAGTAGAGAAGTACTTAAACGCAA CTCTAAAGGAAATGACTGAAGCACCAAGAGTTGAAGATCTGTGCTGTACGTTATATGACATGCTTGCTTC TGCTAAAAGCGGGGATGAGCTTCAGGATGAGCTTTTTGAATTGCTGGGACCTGGAGGACTTGATTTGATT GAGAAGCTACTCCAGAACAGAATTACAATCGTAGATCGATTTCTTAATTCCTCAAGTGATCATAAGTTCC AAGTTCTTCAAGATAATTGTAAAAAAATTTTAGGAGAGAATGCGAAGCCTAATTATGGTTGTCAAGTCAC GATTCAGTCCGAACAAGAAAAGCAGTTAATGAAACAGTATCGGCGTGAGGAAAAAAGAATTGCCAGGAGA GAAAAGAAGGCTGGGGAAGATGGGGAAGTTTCTGGAGAAGGACTTTTGCCCTTTGATCCTAAGGAACTCC GATTACAGAGAGAACATGCGCTTCTGAATGCTAGAAGTGCTCCAATTTTGGGAAGGCAGAGAGACACGGA GGTTGAGAAAATTCGTTATCCTCATGTTTATGATTCCCAGGCTGCAGCCCGAGAAACATCAGCATTTATA GCTGGGGCCAAGATGATTTTGCCAGAAGGCATCCAGAGAGAGAATACCAAGCTGTATGAAGAAGTAAGGA TCCCCTATAGTGAACCAATGCCCGTTGGGTTTGAGGAGAAACCAGTTTATATCCAGGATTTAGATGAGGT GGGACAGTTGGCTTTTAAAGGAATGAAGAGACTCAATAGAATCCAGTCTATAGTATTTGACACAGCCTAC AACACCAACGAAAACATGCTCATCTGTGCCCCTACTGGAGCTGGGAAGACCAATATTGCAATGCTCACTG TCTTACATGAAATCCGCCAACACTTTCACCAAGGTGTTCTTAAAAAGAATGAGTTTAAGATTGTGTATGT TGCTCCAATGAAAGCCTTGGCAGCTGAAATGACGAATTACTTCAGCAAACGTCTAGAGCCACTGGGCATT GTTGTGAAAGAATTGACTGGAGATATGCAGTTGTCAAAAAGTGAAATTCTACGAACTCAGATGCTTGTGA CCACACCAGAAAAATGGGATGTAGTAACAAGAAAGAGTGTTGGGGATGTTGCTCTTTCCCAAATTGTAAA ACTCCTGATTCTCGATGAGGTTCACTTGCTGCATGAAGACAGAGGCCCAGTGCTAGAAAGCATAGTTGCG AGGACCTTACGGCAGGTTGAATCCACACAGAGTATGATAAGAATCCTTGGCTTATCTGCAACCTTGCCCA ACTACCTGGATGTTGCTACATTTTTACATGTTAACCCCTACATTGGTCTTTTCTACTTTGATGGCCGTTT TCGACCTGTACCTCTGGGACAGACATTTTTAGGGATTAAAAGTGCAAATAAGATGCAGCAGTTAAATAAC ATGGATGAAGTGTGCTACGAAAGCGTTCTGAAGCAAGTAAAGGCTGGACACCAGGTGATGGTATTTGTAC ATGCTCGAAATGCCACTGTAAGGACAGCTATGTCTCTAATAGAGAGAGCAAAAAACAGTGGCCAGATCTC CTGTTTTCTCCCCACCCAAGGACCAGAGTATGGGCATGCATTGAAACAGGTACAAAAGTCAAGAAATAAG CAAGTGCGAGAGTTATTCTCAGATGGCTTTAGCATTCATCATGCCGGGATGCTTCGGCAAGACAGAAATT TGGTTGAAAATTTGTTCTCAAATGGGCACATCAAAGTCCTGGTTTGCACAGCCACCCTAGCCTGGGGTGT CAATCTCCCTGCACATGCCGTCATTATTAAGGGAACACAAATCTATGCTGCCAAAAGAGGCTCCTTTGTT GACCTTGGAATTTTAGATGTCATGCAGATATTTGGCCGAGCCGGACGACCGCAATTTGACAAATTTGGGG AAGGAATCATTATAACCACACATGATAAACTCAGCCATTACCTCAGTTTGCTCACTCAACAAAACCCGAT TGAGAGTCAGTTTCTGGAAAGTCTTGCAGATAACCTAAATGCAGAGATTGCACTAGGAACAGTCACCAAT GTGGAAGAGGCGGTGAAGTGGATGAGTTACACGTACCTCTACGTGCGGATGAGAGCAAACCCACTCGCCT ACGGCATCAGTCACAAAGCATACCAGATGGACCCAACGTTAAGAAAGCACCGAGAACAGCTCCTCATTGA AGTTGGACAAAAGCTGGATAAAGCTAGGATGATTCGCTTTGAAGAACGAACTGGCTATTTTTCTTCCACT GATTTGGGTAGAACTGCCAGCCACTACTATATTAAATACAACACCATTGAGACATTCAATGAACTCTTTG ATGCACACAAAACAGAAGGTGATATCTTTGCTATTGTCTCCAAAGCTGAAGAATTTGATCAAATTAAGGT CAGAGAAGAAGAAATAGAAGAATTAGATGCCTTGTTAAACAATTTCTGTGAACTCTCAGCTCCAGGAGGT GTGGAAAATAGTTATGGGAAAATAAACATCCTACTTCAGACCTACATCAGCCGAGGAGAGATGGACAGTT TCTCCCTCATATCAGATTCCGCATATGTTGCCCAGAATGCAGCTAGAATTGTCCGTGCTCTCTTTGAAAT TGCACTGAGGAAACGTTGGCCTACCATGACCTACAGGCTTTTGAATCTTAGTAAAGTCATTGACAAGAGA CTCTGGGGTTGGGCTAGCCCTTTGAGACAATTTTCTGTGTTACCACCACACATCCTAACAAGATTAGAAG AAAAAAACCTTACAGTGGATAAACTCAAAGACATGAGAAAAGATGAAATAGGTCACATTTTGCATCATGT GAATATTGGACTGAAGGTCAAACAGTGTGTTCATCAGATTCCTTCTGTTACAATGGAAGCTTCCATTCAG CCCATCACACGGACTGTCCTCCGAGTCTCACTGAACATCTACCCTGATTTCTCTTGGAATGATCAGGTCC ATGGCACAGTAGGGGAACCGTGGTGGATCTGGGTAGAAGATCCTACAAACGACCATATTTATCACTCGGA GTACTTTCTAGCTCTTAAAAAACAGGTTATTAATAAAGAAGCTCAACTTCTGGTATTTACAATCCCTATT TTTGAGCCTTTGCCCTCTCAGTACTACATTCGAGCAGTATCTGATAGATGGCTAGGAGCTGAAGCTGTTT GCATTATCAACTTTCAACATCTGATTCTACCAGAGAGACATCCTCCCCATACAGAATTATTGGACCTTCA GCCTTTGCCAGTCACAGCCTTGGGGTGCAAAGCATATGAAGCTCTGTATAACTTCAGCCACTTCAACCCG GTGCAGACACAGATATTCCACACGCTGTATCACACTGACTGTAACGTCCTACTTGGAGCCCCCACGGGGT CAGGAAAGACAGTTGCAGCTGAACTCGCCATTTTCAGAGTCTTCAACAAGTATCCCACTTCCAAGGCAGT ATATATTGCACCCCTCAAAGCTCTAGTACGTGAAAGAATGGATGATTGGAAAATTAGAATAGAAGAAAAA CTTGGTAAAAAAGTTATTGAACTGACTGGGGATGTGACTCCAGACATGAAATCCATTGCTAAGGCTGACC TTATCGTCACTACACCAGAAAAGTGGGATGGCGTCAGTCGAAGCTGGCAGAACAGGAGCTACGTTCAGCA GGTCAACATTCTCATCATAGATGAGATCCACCTGCTAGGTGAGGAGAGAGGTCCAGTTCTAGAAGTCATT GTATCTCGAACAAACTTTATCTCATCGCACACAGAGAAGCCTGTTAGAATAGTCGGACTGTCCACTGCCT TAGCCAATGCTAGAGACCTTGCTGACTGGCTCAACATTAAGCAGATGGGTTTGTTTAACTTCCGACCTTC TGTGCGCCCTGTTCCACTGGAAGTTCACATTCAAGGTTTCCCAGGACAACATTACTGCCCACGCATGGCT AGTATGAACAAGCCTGCATTCCAGGCAATTAGAAGTCATTCTCCAGCCAAACCTGTTTTGATATTTGTCT CCTCAAGACGTCAAACACGGCTTACGGCTTTAGAGTTGATAGCTTTCCTGGCCACTGAAGAAGACCCAAA GCAGTGGTTAAACATGGACGAGCAAGAGATGGAGAACATCATTGCAACAGTGCGAGATTCCAACCTTAAG CTGACTCTAGCTTTTGGAATAGGAATGCATCATGCTGGACTACATGAAAGAGACCGAAAAACTGTGGAAG AGCTGTTTGTGAACTGTAAAGTGCAGGTTCTCATTGCGACAAGCACGTTAGCATGGGGTGTAAACTTTCC AGCACACTTAGTCATTATTAAGGGAACTGAATACTACGATGGAAAGACAAGACGCTATGTGGATTTCCCC ATCACAGATGTACTGCAGATGATGGGGCGAGCTGGGAGACCTCAGTTTGATGACCAGGGCAAAGCTGTAA TTCTAGTTCATGATATAAAAAAGGATTTTTATAAAAAGTTTCTTTATGAACCTTTCCCAGTGGAATCGAG CTTATTAGGAGTGTTGTCTGACCACTTAAATGCAGAGATTGCGGGAGGTACAATAACATCAAAGCAAGAT GCCTTGGATTATATCACTTGGACTTACTTTTTCCGACGTCTTATCATGAATCCCAGCTACTACAACTTGG GGGACGTCAGCCAGGACGCCATAAACAAGTTCCTGTCACATCTGATTGGGCAATCTCTGGTTGAGTTGGA GCTTTCCCACTGCATTGAAGTTGGAGAGGACAATCGCAGCATTGAACCACTGACATGTGGCCGAATTGCC TCCTATTACTATCTGAAGCACAAGACAGTCAAAATGTTCAAGGACCGTTTAAAGCCTGAGTGCAGCACTG AAGAATTGCTGTCAATTCTAAGTGATGCGGAAGAATACACAGACTTGCCAGTAAGACACAATGAGGACCA TACGAATAATGAACTGGCCAAATGTCTTCCCATTGAATTAAATCCTCATTCATTTGACAGCCCTCACACC AAAGCCCATCTCTTGCTTCAAGCACATCTCAGCCGAGCCATGCTGCCCTGCCCAGACTATGATACTGATA CCAAAACAGTCTTAGATCAAGCTCTCAGAGTATGCCAGGCAATGCTGGATGTGGCTGCAAGCCAAGGATG GCTAGTGACTACCCTGAATATCACCCACCTGATTCAGATGGTGATCCAAGGCCGATGGCTAAAGGACTCG TCTCTTCTTACCATACCAAACATAGAACAGCACCATCTTCACCTTTTCAGGAAGTGGAAACCACCTGTAA AGGGCCCACATGCTAAGTGCCGAACATCCATTGAGTGCCTTCCTGAGCTGATCCATGCCTGTGAAGGGAA GGAGCATGTGTTCAGCTCCATGGTAGAGAAGGAGTTACAGCCTGCGAAAACAAAGCAGGCATGGAATTTC TTATCTCACTTGCCCGTGATAAATGTTGGCATAAGTGTTAAAGGCTCCTGGGATGACTCAGTTGAAGGAC ATAATGAACTCTCCATCTCAACTCTGACAGCAGACAAACGGGATGAAAACACATGGATCAAGTTGCATGC TGATCAGCAGTATGTGCTTCAAGTCAGCCTACAGAGAGTCCATTTTGAGTTCCACAAGGTAAAACATGAG AGTCACGCAGTTACTCCTCGATTTCCAAAATTAAAAGATGAAGGGTGGTTTTTGATATTAGGAGAAGTGG ATAAGAGAGAGCTGGTGGCTGTAAAACGCGTAGGATTTGTTCGAACTCATCACGAGGCTTCCATCTCTTT CTTTACTCCGGAAGCACCTGGAAGATATATCTTTACCCTGTATCTCATGAGTGACTGTTACCTCGGCCTA GACCAGCAGTATGACATCTTTCTCAATGTCACAAAAGCAGACATCTCCACCCAGATCAACACAGAAGTCC CTGATGTCTCAACTGACTTGGCATAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001277057
Insert Size	6606 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277057.1, NP_001263986.1
RefSeq Size	7446 bp
RefSeq ORF	6606 bp
Locus ID	309887

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.