Myo7b (NM_001191941) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1561153
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217747 representing NM_001191941 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGTGTTCCGGCTGGGTGACCATGTGTGGCTGGACCCTCCCTCCAGCAACAAGACTGGTGTGGCCA TTGGAGGCATTGTCAAAGAAACAAAACTGGGGAAGACCTTGATTGAGGATGACGAGGGCAAGGAACACTG GATCTCTGCTGAAGACCTCAGCACCCTCAGGCCTATGCACCCCAACTCGGCCCAAGGAGTGGATGACATG ATTCGCCTGGGGGACCTGAATGAGGCCGGTGTGGTACACAACCTCCTGATCCGATACCAGCAGCACAAGA TCTATACCTACACGGGTTCCATCCTGGTGGCGGTGAACCCATTCCAGATGCTGCCCCTGTACACCCTGGA GCAGGTACAAATCTACTACAGCCGCCACATGGGCGAGCTGCCTCCCCATGTCTTTGCCATTGCCAACAGC TGCTACTTCAACATGAAGAAGAACAAGAGGGACCAGTGTTGCATCATCAGTGGTGAGTCTGGCGCTGGGA AGACCGAGACCACCAAGCTCATCCTTCAGTTCCTGGCCACAGTCAGCGGCCAGCACTCGTGGATCGAGCA GCAGGTCCTGGAGGCCAACCCCATCCTGGAAGCCTTTGGCAATGCCAAGACCATCCGCAATGACAACTCC AGCCGCTTTGGGAAGTACATCGACATCCACTTCAACTCTAGCGGGGTGATCGAGGGGGCAAGCATTGAGC ACTTCCTACTGGAAAAGTCGAGGGTCTGCCGGCAGGCTGCGGAGGAACGGAACTACCATATTTTCTACTG CATGCTGATGGGGATGAGCCTGGAAGAGAAGAACATGCTGGGTCTGGGCATGCCCTCTGAGTACCACTAC CTGACCATGGGAAACTGCACCTCCTATGAGGGGCTCAGTGATGCCAAGGACTACGCACACGTTCGCTCGG CCATGAAGATCCTCCAGTTCTCGGACTCGGAGAACTGGGACATCAGCAAGCTGCTGGCAGCCATCCTCCA CCTGGGGAATGTGGGGTTTATGGCTGCCGTCTTTGAGAACCTGGATTCCTCTGATGTGATGGAAACTCCG GCCTTCCCCTTTGCAATGAAGTTGCTAGAGGTACAGCACCAGGCTCTCCGAGACTGTTTGATCAAACACA CAATCCCTATCCTTGGAGAGTTTGTCTCCAGGCCCTTGAACATCGCGCAGGCTGCAGACCGGAGAGATGC CTTTGTCAAGGGCATCTACGGGCACCTCTTCCTGTGGATCGTCAAGAAGATCAATGCTGCGATCTTCACT CCCCAAGCCCAGGATCCCCAAAATGTGCGGAGGGCCATCGGTCTCCTGGACATATTTGGCTTTGAAAACT TTCAAAACAACAGCTTCGAGCAACTCTGCATCAATTTCGCCAACGAGCACCTTCAGCAATTCTTCGTGAA GCACGTGTTCACCATGGAGCAGGAGGAGTACCTGTCTGAGAACATCACTTGGAACTACATCCACTACACG GACAACCAGCCCATCCTAGACATGCTGGCCCTCAAGCCCATGAGCATCATCTCTCTCCTGGATGAGGAGA GCCGCTTCCCACAGGGGACAGATGTCACTATGCTGCAGAAACTGAACAGCATCCATGCCAACAACAAGTC CTTCCTGAGACCCAAGAGCATCCATGACACCCGATTTGGGATCGCACACTTTGCGGGCGATGTATACTAC CAGGCAGAAGGCTTTCTGGAGAAGAATCGAGATGTGCTGAGCACTGACATCCTCATCCTGATTCACTCCT CAAAAAACAAGTTCCTGAAGGAGATATTTAACCTGGACTTACCACAGACAAAGCTGGGGCACGGCACCAT CTGCCAGGTGAAGACGAAGACAGGAGGCCAGATCTTCAAGCCATCAGACTCCACCAAGCGCTCCGTCACC CTCTCGAGCCAGTTCAAGCAGTCTCTGGAGCAGCTGATGAAAATCCTGACCAATTGCCAGCCCTACTTTG TCCGCTGCATCAAACCCAATGAGTACAAGAAACCACTGCTGTTTGACCGAGAGCTGTGCATTCAGCAGCT GCGTTATTCCGGCATGATGGAGACCGTGCACATCCGCAAGTCAGGCTTTCCCATCCGCTACACATTCGAC GAGTTCTCACAGAGGTTCCGCGTGCTGCTGCCTAGTCCGGAACGTGTGCAGTTTCAAAACAAGCACCGCC AGATGACCTCGCGCATTGCTGACCTGTGCCTGGGGACGGACAAAGAGTGGAAAATGGGAAAGACCAAAAT CTTCCTGAAGGATCATCAGGACACAATGCTGGAGATCCAGAGGAGCCAGGCCCTGGATGGAGCAGCGATC CGAATCCAGAGAGTCCTTCGGGGACACAAATACAGGAAGGAGTTCCTGAGGCAGAAACGGGCGGCTGTGA CCCTCCAAGCAGTGTGGCGAGGCCATAACCAAAGAAAGAATTTCAAGCTGATCCTCATGGGTTTTGAACG GCTGCAAGCCATTGCCCGGAGCCACCTGCTGATGAGGCAGTTCCAGACCATGCGACAGAAGATAGTGCAG CTGCAGGCCCGCTGCAGGGGCTACCTGGTGCGACAGCAAGTGCAGGCCAAGAGGAGAGCAGTGGTGATCA TTCAAGCCCATGCCAGAGGCATGGTGGCCCGGAAGAGCTACTGGCAACAGAAAAGCAGTGGACCTCCAGT CATCCTGGCTAAGGAGCCAAAGGGCCAAGGTGTGGCCCTTGACAGGAAGCGTAAGTCCATCTATGACACC GTCACTGACACAGCAATGGTGGAGAAAGTGTTTGGCTTCCTCCCAGCTATGATTGGAGGTCAGGAGGGTC CAGCCCCAACACGCTTTGAGGATTTGGAAGTAAAGACCCAGAAACTTCATGAGGTTGATCTGGACACAGT GCCCATGACGGAGATGCCAGGAGAGGATGTGGATGGTCTGGCTGAGTATACCTTCCCCAAGTTTGCTGTA ACTTACTTCCAGAAATCAGCCAGTCACACCCACATCCAGAAGCCCCTTCGATACCCACTCCTCTACCATG AAAATGACACTGACCACTCGGCAGCTCTGGCTGTTTGGATCATCATCCTGAGGTTCATGGGTGACCTTCC AGAACCACAGGCCTATGGCAGGAGCAGCCTGACTGGCAGCTCAGTTATGCGGCAGATCCATGACAGGCTG GACAAGGACAGTGTCACTCAGGGTCCACAGCACAACAGATCCACACAGGTGGCCAGCCAGCAGAACATTG GTGAAGAGGTGTTCAAGTTTGATGGCCCTATCTCAGACAGACCCATGAGTAACTTGGAGAAGGTGCACTT CATTGTGGGCTACGCCATCATGCGTCCTGGACTCAGAGATGAAATCTACTGCCAGATCTGCAAGCAGCTC TCCGAGAACTACAAAACAAGTAGCCGGGCTCGGGGCTGGATCCTGCTCAGCCTCTGTCTGGGCTGCTTCC CACCCTCAGAGAGGTTCATGAAGTATCTGCTGAACTTCATTAGTCAAGGGCCAGCCAGCTATGGGCCCTT CTGTGCCGAACGCCTGCAACGCACCTTTGCCAATGGGGTGCGTGCAGAACCTCCCACCTGGCTGGAGCTG CAGGCTGTCAAGTCCAAGAAGCACATCCCGATCCAGGTCATCCTGGCAACCGGAAGGAGCCTGACCATCT CCGTGGATTCTGCCTCCACATCTCGAGAAATCTGCCAGCAAATTGCTCAGAAACAGGGCCTCAGGGACAA GCTGGGCTTCTCCCTCCAGGTCGCTGTATATGACAAGTTCTGGTCCTTGGGCAACAGCTGTGACCATGTG ATGGATGCTGTGGCCCAGTGTGAGCAACTGGCCTGGGAGAGGGGAGAGAGCCAGCGCCAGGCCCCCTGGC GCATCTACTTCCGGAAGGAGTTCTTCACCCCGTGGCATGACTCCCGAGAGGACCCCGTGAGCACTGAGCT CATTTACCACCAAGTCCTCCGGGGAGTGTGGTCTGGCGAGTATAACTTTGAGAAGGAAGAGGACCTGGTA GAGCTGCTAGCCCGGCACTGCTATGTGCAGCTTGGGGCCACAGTGAAGAGTAATGCTGTCCAGGAGCTGC TACCCAGCTGTGTTCCTTCCAAGCTGTACCGCACCAAGTCTCCAGAGAAGTGGGCTAGCCTTGTCATCGC TGCCCATGCCAAGGCCCCGTATACCCAGTCGAAAGCCACACCATTGGAGGTGAAGGAGCAGACAGTGGAG GCTGCCCGTCTTCTGTGGCCACTGCTCTTCTCCCGGCTATTTGAAGTCACCACACTCTCCGGCCCCCGCC TTCCCAAGACGCAGCTGATTTTAGCCATTAACTGGAAGGGGATGTACTTCCTGGATCAGAAGGAGAGGAC ACTGCTGGGGTTGTCCTTCGCAGAGGTCATGGGTCTGATCGCCAACAGGGACGCCCCAGGTGGGAAGAGT CTGCTTCTGTCCACACTGCATGAAGAGTACGAGTTCGCCTCCCCCAGCAGCGTGGCCATTGCAGAGATGG TGGCCTTGTTCCTGGCAGGCTTGAAGGAGAGATCTGTGTTCGCCATGGCCCTGCAGGACAGGAAGGCCAC AGATGATGTCACCCTTCTGCCCTTCAAGAAAGGGGACTTACTGATCCTGACCAAGAAACAGGGACTGTTG GCCTCAGAGAACTGGGTCCTGGGCCAGAACGACAGAACCGGCAAGACAGGACTGGTGCCCACGGCCTGCC TCTACACCATCCCCTCAGTCACCAAGCCCTCAGCCCAGCTGCTGAACTTGCTGGCCATGTCGCCAGAGAA ACGGAAGCTGGCAGCCCAGGAGGTGCAGGCTTCGGAGCCACCGCTAGAGGAGCAGCTCACAGAGTTACCG TACACCCTGGAGGAGTTCTCCTACCAGTATTTCAGGGCTCCAGAGAAAGAGACCATCAGCAGGGCGGCGA TGCCCATGGCCCGAAGCCGAGGCCATCTATGGGCCTACTCTCCCGAGCCGCTGCGCCAGCCACTGCTCAA ATCTGTCCACGACAAAGCCAAGCTTCGAGATTCAGCCTGCCAGATCTTCCTTGCCATCCTCAAGTACACA GGCGACTACCCATCCCGGCAGTCCTGGCACAGCCTGGAGCTCACAGACCAGATGTTCTCGCTAGCCCTGA AGGAGCCAGCCCTTCAGGACGAGCTCTACTGCCAAATCCTGAAGCAGTTGACCCACAACTCCATCAGGTT CAGTGAAGAGAGAGCCTGGCAGCTACTGTGGCTCTGCACAGGCCTCTTCCCACCTGGCAAGGCGCTGCTG CCCCATGCCCAGAAGTTCATAGACTCCCGGAAGAAGAAGCCACTAGCTCTCGACTGCAGCCGCAGGCTCC ACAGGATTCTAAGGGTGGGACCTCGGAAGCAGCCCCCACACCATGTAGAGGTGAAGGCCGCTGAGCAGAA TGTCTCCAAACTACACCATGAGGTCTACCTCCCCAATGACACCAATAAGACCTTGGAGGTGAGCAGCAGT AGCCGGGTGCGAGACCTATGTGAGGGCATCGCCACTAGGCTCCAGCTGGCCTCCTGGGATGGCTGCAGCC TCTTCATCAAGATCACAGACAAGGTCATCAGCCTGAAGGAAGGAGACTTCTTTTTTGACAGCTTGAGGCA GGTATCTGACTGGGTGAAGAAGAACAGGCCCCAGAAAGAAGGGGCCTCCACGACACTCCCCTACCAGGTG TTCTTCATGCGGAAACTGTGGCTCAATGTGACCCCTGGGAAGGACGTGAATGCAGACACCATACTCCATT ACCACCAGGAGCTACCCAAGTACATGCGTGGGTTTCACAAGTGCTCCCGGGAAGACGCCATCTACCTGGC AGGCCTCATCTATAAAATCCAGTTTGGCAGCGATCGATCTCAAATGGCCAGTGTCTCGAAGATCCTGAAG GAGCTGGTACCTCAGAACCTCACACGCCTGATGTCCTCAGAGGAATGGAAAAAGAGCCTCCTTCTGGAAT GTGACAAACACAAGAATAAGTCAGTGGCCGAGGCCAAGGTGGAATTCCTGAAGCGGATCTACCGATGGCC TACCTTTGGATCTGCCTTCTTTGAGGTGAAGCAAACCTCTGAGCCTTCCTACCCAGACATCCTCCTCATT GCCATCAACCGACATGGGTTACTGCTCATCCACCCCAAGACCAAGGAACTGCTGGACACCTTCCCTTTCA CCAAGATCTCCAGCTGGAGTAGCGGCAACACCTACTTCCATATGGCCTTGGGGAGCCTGGGCCAGGGCAG TCGTTTGCTGTGTGAGACCTCTCTGGGATACAAGATGGACGACCTGCTCACTTCATACGTACAGCAGATC CTGAGTGCAGTGAACAAACATCGGGGCTTCAGGGCCCCTGCTCCGGCTCAACCAGATCACGAAAATCTCT GGAGACTTCCTGTGTGCCCCCCAGACAGGCCCCAGGGCACAGAATCTCCCACCCAGGCCTGCCGGAAGTG CTGTGTTCTGTCCATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191941
Insert Size	6459 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191941.1, NP_001178870.1
RefSeq Size	6568 bp
RefSeq ORF	6459 bp
Locus ID	498834

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