Taf1 (NM_001191723) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1562050
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217708 representing NM_001191723 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCAGGGTGGGCTGGACTGCTGCCCGAAGACGGTTCTGGTAGCCCTTCCGCCGTCATGTCAGACA CAGACAGCGATGAAGATTCTGCTGGTGGAGGCCCGTTTTCTTTGACTGGGTTCCTGTTCGGCAACATAAA TGGAGCAGGGCAGCTGGAGGGTGAAAGCGTCTTGGACGATGAGTGCAAGAAGCATCTTGCAGGCTTGGGG GCATTGGGCCTTGGTAGCCTGATCACTGAACTCACAGCAAATGAAGAATTGTCTGGGACTGATGGTACCC TGGTAAATGATGAAGGATGGATTAGGAGTAGAGAAGATGCTGTGGACTACTCAGACATCAACGAGGTGGC AGAAGATGAAAGTCGAAGATATCAGCAGACAATGGGGAGCTTGCAACCCCTTTGCCACACAGATTATGAT GAAGATGACTATGATGCTGATTGTGAAGATATTGATTGCAAATTGATGCCTCCCCCACCTCCACCCCCAG GACCAGTGAAGAAAGAGAGGGACCAAGATGGTATTACTGGTGTGTCTGAAGATGGAGAAGGCATCATCTT GCCCTCCATCATTGCCCCTTCCTCTTTGGCCTCAGAGAAAGTGGACTTCAGTAGTTCCTCTGACTCAGAA TCTGAGATGGGACCTCAGGAAGCAACACAGTCAGAATCTAAGGATGGAAAGCTGACCCTACCATTGGCTG GGATTATGCAGCATGATGCCACCAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTAGACCTGG AAAGGTGTTACGCTTTTTACGGCTTTTTGGACCAGGGAAGAATGTCCCATCTGTTTGGAGGAGTGCTCGG AGAAAGAGGAAAAAGAAGCACCGTGAACTTATACAGGAAGAGCAGATCCAGGAAGAGGAGTGTTCAGTAG AATTAGAAGTCAACCAGAAATCTCTGTGGAACTATGACTATGCTCCACCACCACTTCCAGAGCAGTGTCT CTCTGATGATGAAATCACAATGATGGCTCCTGTGGAGTCCAAGTTTTCCCAGTCAACTGGAGATACAGAT AAAGTAATGGATACCAAACCAAGAGTGGCAGAATGGCGTTATGGACCTGCCCGGCTATGGTATGATATGC TAGGTGTTCCTGAAGATGGCAGTGGCTTTGACTATGGCTTCAAAATGAAGAAGACTGAACATGACTCTAC AATGAAATATAACATAATGAAGAAACTAAGAAAACTTGAGGAAAACAATGGCGTTGATCTTCTGGCAGAT GAAAACTTCCTAATGGTGACACAACTGCATTGGGAAGATGATATCATCTGGGATGGGGAGGATGTCAAAC ACAAAGGAACAAAACCTCAGCGTGCAAGTTTGGCAGGTTGGCTTCCCTCCAGTATGACTAGGAATGCCAT GGCCTACAATGTTCAGCAAGGTTTTACAGCTACATTGGATGATGATAAACCATGGTACTCCATTTTCCCC ATTGATAATGAAGATCTGGTATATGGACGTTGGGAGGACAATATCATTTGGGATGCTCAGAACATGCCTA GGATTTTGGAGCCTCCTGTTTTGACACTTGATCCCAATGATGAGAACTTGATTTTGGAGATCCCCGATGA GAAGGAAGAAGCTACTTCTAATTCCCCTTCTAAGGAGAATAAGAAAGAATCATCTCTGAAGAAGAGTCGA ATTCTCTTGGGAAAAACAGGAGTTATCAAGGAAGAGCCACAGCAGAACATGTCTCAGCCAGAAGTGAAAG ATCCATGGAATCTCTCCAATGATGAGTATTACTATCCCAAGCAACAGGGTCTTCGAGGTACTTTCGGAGG GAATATTATCCAGCACTCAATTCCAGCTGTAGAATTACGGCAGCCCTTCTTTCCCACCCACATGGGACCC ATCAAACTCCGGCAGTTCCATCGCCCACCTCTAAAAAAGTACTCCTTTGGGGCACTATCTCAGCCAGGCC CACACTCAGTCCAGCCTTTGTTGAAGCATATCAAAAAGAAGGCCAAGATGAGAGAACAAGAGAGACAAGC CTCGGGTGGTGGAGAGATGTTTTTTATGCGTACACCTCAGGACCTTACAGGCAAAGATGGAGACCTTATT CTTGCTGAATACAGTGAAGAAAATGGACCATTAATGATGCAAGTTGGCATGGCAACTAAGATAAAAAACT ACTATAAACGGAAACCTGGAAAAGATCCTGGAGCCCCAGATTGCAAATATGGAGAAACTGTTTATTGTCA TACATCACCTTTCCTGGGTTCTCTTCATCCTGGCCAGTTACTGCAGGCATTTGAAAACAACCTTTTTCGT GCTCCAATTTATCTTCATAAGATGCCAGAGTCTGACTTCCTGATCATTCGCACAAGGCAGGGTTACTATA TTCGAGAATTGGTGGATATTTTTGTTGTGGGCCAGCAGTGTCCCTTGTTTGAAGTTCCTGGTCCTAATTC CAAAAGGGCCAATACACACATCAGAGACTTTCTGCAGGTTTTTATTTACCGGCTCTTTTGGAAGAGTAAA GATAGGCCACGGAGGATACGGATGGAAGATATAAAGAAAGCCTTTCCTTCACATTCAGAAAGCAGCATCC GGAAGAGGCTAAAGCTCTGTGCTGACTTCAAACGTACAGGGATGGATTCAAACTGGTGGGTGCTGAAGTC TGATTTTCGTTTACCAACGGAAGAGGAGATCAGAGCTATGGTCTCTCCAGAACAGTGCTGTGCTTATTAT AGCATGATAGCTGCAGAACAGAGACTAAAGGATGCTGGCTATGGAGAGAAGTCCTTTTTTGCTCCTGAGG AAGAAAATGAAGAAGATTTCCAGATGAAGATTGATGATGAAGTTCGTACTGCTCCTTGGAATACTACAAG GGCTTTCATTGCTGCCATGAAGGGCAAATGTCTCCTGGAGGTGACTGGAGTAGCAGATCCTACAGGTTGT GGTGAAGGGTTCTCCTATGTGAAGATTCCAAACAAACCAACACAGCAGAAGGATGATAAGGAGCCTCAGC CAGTGAAGAAAACAGTCACAGGAACAGATGCAGACCTTCGTCGCCTATCTTTGAAAAATGCCAAGCAACT TCTTCGTAAATTTGGTGTGCCTGAGGAAGAGATTAAAAAGTTGTCCCGCTGGGAAGTTATTGATGTGGTA CGCACAATGTCAACAGAACAGGCTCGTTCCGGAGAGGGGCCCATGAGTAAATTTGCTCGTGGATCAAGGT TTTCTGTGGCTGAGCATCAAGAACGTTACAAAGAGGAATGTCAGCGCATCTTTGACCTACAGAACAAGGT TTTGTCTTCAACAGAGGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAAGACAGTGACTTTGAAGAA ATGGGAAAAAACATTGAGAATATGCTGCAGAACAAGAAAACAAGCTCTCAGCTGTCCAGGGAACGGGAGG AGCAGGAACGAAAGGAACTCCAGCGGATGCTACTGGCAGCAGGCTCGGCAGCAGGAGGAAACAACCACAG AGATGATGACACAGCCTCGGTGACTAGCCTTAACTCTTCTGCCACTGGCCGCTGTCTCAAGATTTATCGA ACATTTCGAGATGAGGAGGGGAAGGAATATGTTCGTTGTGAGACAGTCCGAAAAGCAGCTGTTATTGATG CTTATGTACGCATACGGACCACAAAAGATGAAGAATTCATTCGAAAATTTGCCCTTTTTGATGAGCAACA CCGAGAAGAGATGAGAAAAGAACGGCGGAGGATTCAGGAGCAACTGAGGAGACTTAAACGAAACCAGGAA AAGGAGAAACTGAAGGGTCCTCCTGAGAAAAAGCCCAAAAAGATGAAGGAACGTCCTGACTTAAAACTAA AATGTGGAGCATGTGGTGCAATTGGACACATGAGGACAAATAAATTCTGCCCTCTTTATTATCAAACAAA TGCACCACCTTCTAATCCTGTTGCAATGACAGAGGAACAGGAAGAAGAGCTGGAAAAGACAGTCATTCAC AATGATAATGAAGAACTTATCAAGGTCGAAGGGACCAAGATCGTTTTGGGAAAACAGCTAATTGAGAGTG CGGATGAAGTACGCAGAAAATCTCTGGTTCTGAAGTTCCCTAAACAACAACTTCCTCCCAAGAAAAAGCG TCGGGTTGGAACCACTGTTCACTGTGACTATTTGAATAGACCTCATAAATCCATCCACCGACGCCGGACA GACCCTATGGTGACACTGTCTTCTATCTTGGAATCTATTATCAATGACATGAGAGATCTTCCAAATACAT ACCCTTTCCATACTCCAGTTAATGCAAAAGTTGTAAAGGATTACTACAAAATCATCACTCGGCCAATGGA TCTACAAACACTCCGTGAAAATGTGCGCAAACGTCTTTATCCATCTCGAGAAGAATTCAGAGAACATTTG GAATTAATTGTGAAAAATAGTGCAACTTATAATGGGCCAAAACATTCACTGACTCAGATTTCTCAATCCA TGCTGGATCTCTGTGATGAAAAACTCAAAGAGAAAGAGGATAAGTTGGCACGTTTAGAGAAAGCTATCAA CCCCTTGCTTGATGATGATGATCAAGTAGCATTTTCTTTTATTCTGGATAACATTGTCACTCAGAAAATG ATGGCAGTTCCAGATTCTTGGCCATTTCATCACCCAGTTAATAAGAAGTTTGTTCCAGATTATTACAAAG TGATTGTCAATCCAATGGATTTGGAGACTATACGTAAGAATATCTCCAAGCACAAATACCAGAGTCGAGA GAGCTTTTTAGATGATGTAAACCTGATTCTGGCCAACAGTGTTAAGTACAATGGGCCTGAGAGTCAGTAT ACTAAGACTGCTCAGGAGATTGTGAACGTTTGTCACCAGACATTAACTGAGTATGATGAACATTTGACTC AACTTGAGAAAGATATTTGTACAGCTAAAGAAGCAGCTCTGGAGGAAGCAGAATTAGAAAGTCTAGACCC AATGACTCCAGGACCTTATACCCCTCAGGCTAAGCCTCCTGATTTATATGATAACAGCACATCCCTCAGT GTTTCTCGAGATGCTTCGGTGTATCAAGATGAGAGCAATCTGTCTGTCTTGGATATTCCCAGTGCTACTT CAGAAAAGCAGCTAACACAGGAAGGTGGAGACGGAGATGGTGATCTTGCAGATGAAGAGGAAGGAACTGT ACAACAGCCCCAAGCCAGTGTCCTGTATGAGGATTTGCTTATGTCTGAAGGTGAAGATGATGAAGAAGAT GCTGGGAGTGATGAAGAAGGAGATAACCCTTTCTCTGCTATTCAGCTAAGTGAAAGTGGAAGTGACTCTG ATGTGGAATCTGGCAGCATAAGACCCAAACAGCCCCGCATGCTTCAAGAGAATACAAGGATGGGCATGGA AAATGAAGAAAGCATGATGTCCTATGAGGGAGATGGTGGGGAGGCTTCCCGTGGTTTGGAGGATAGCAAC ATCAGTTATGGGAGCTATGAGGAGCCTGATCCCAAGTCGAACACCCAGGATACAAGCTTCAGCAGCATCG GTGGGTATGAGGTATCAGAGGAGGAAGAAGATGAGGAAGAGCAGCGCTCTGGGCCCAGCGTTCTAAGCCA GGTCCAACTCTCAGAGGACGAGGAGGACAGTGAGGATCTCCACTCCATTGCTGGAGACAGTGACTTGGAC TCTGATGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191723
Insert Size	5682 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191723.1, NP_001178652.1
RefSeq Size	7260 bp
RefSeq ORF	5682 bp
Locus ID	317256

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