Pcdh15 (NM_001271377) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217693 representing NM_001271377 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCCACAGTTTGCTCTGTGGAAGTGTATGCCCCATGGGATCCTCCTTGCCTCACTCTTGATAGTCA GCTGGGGCCAATATGATGATGACTGGCAATATGAGGATTGTAAACTAGCTAGGGGAGGACCACCAGCTAC CATTGTGGCCATTGATGAAGAGAGTCGGAATGGTACAATTCTGGTGGACAACATGTTGATTAAGGGGACT GCCGGAGGACCAGACCCCACCATAGAGCTCTCTTTAAAGGACAACGTGGACTACTGGGTGTTACTGGACC CCGTTAAGCAGATGCTTTTCCTCAACAGTACCGGAAGAGTTCTGGATAGAGACCCACCGATGAACATACA CTCCATTGTAGTCCAAGTCCAGTGTGTCAACAAGAAGGTTGGCACAGTGATCTATCATGAAGTACGCATC GTGGTGCGGGACCGGAATGACAACTCACCCACATTCAAGCATGAGAGCTACTATGCCACCGTGAATGAGC TCACTCCGGTCGGCACCACAATATTCACGGGGTTTGCGGGAGACAACGGAGCCACAGACATAGACGATGG CCCTAACGGACAGATAGAATACGTCATTCAGTACAACCCAGAAGATCCGACATCCAATGACACCTTCGAA ATTCCACTCATGTTGACTGGCAACGTGGTGCTGAGGAAAAGACTCAACTACGAGGATAAGACTCGCTACT ACGTCATCATCCAAGCTAATGACCGTGCACAAAATCTGAATGAGAGGCGAACAACTACTACCACCCTCAC AGTAGACGTTCTAGATGGAGATGATCTGGGACCAATGTTTCTGCCCTGTGTTCTTGTGCCAAATACCCGT GACTGCCGTCCACTCACCTATCAAGCTGCCATTCCTGAACTGAGGACTCCGGAAGAACTGAACCCTATTT TGGTGACCCCACCTATCCAAGCCATTGACCAGGACCGAAACATCCAACCACCATCTGATCGACCTGGCAT CCTCTACTCCATTCTTGTTGGCACCCCTGAGGATTACCCCCGCTTCCTCCACATGCATCCCAGGACTGCA GAACTCACTCTCCTGGAGCCAGTAAACAGAGACTTCCATCAAAAGTTTGATTTGGTTATTAAGGCCGAAC AGGACAATGGCCACCCGCTGCCTGCCTTTGCTAGTCTACACATCGAGATACTGGACGAAAATAATCAGAG TCCATACTTTACAATGCCCAGCTATCAAGGGTACATTCTGGAATCTGCCCCAGTGGGAGCCACCATTTCT GAGAGCCTGAACTTAACCACGCCTCTGAGAATTGTAGCTCTGGACAAAGACATAGAAGACGTGCCACCTG GTGGAGTTCCTACAAAGGACCCGGAGCTCCACCTTTTCCTGAATGATTACACCTCGGTCTTCACCGTGAC ACCCACCGGTATCACCCGCTACCTCACCCTGCTTCAACCTGTGGACAGGGAGGAACAGCAAACCTATACC TTTCTGATAACAGCGTTCGATGGCGTGCAGGAGAGCGAGCCAGTGGTGGTCAATATCCGAGTGATGGATG CCAATGACAACACGCCCACCTTCCCTGAGATCTCCTACAATGTCTATGTTTACACGGACATGAGTCCCGG GGACAGTGTCATCCAGCTGACTGCAGTGGACGCTGATGAAGGCTCTAACGGGGAGATCTCCTATGAAATA CTAGTGGGCAGCAAGGGAGACTTCGTGATCAACAGGACCACGGGGCTGGTGAGCATCGCGCCAGGAGTGG AGCTGGTCGTGGGACAGACGTATGCGCTCACGGTACAGGCTTCGGACAACGCCCCGCCCGCAGAGAGAAG GCACTCCATCTGCACGGTGTACATCGAGGTGCTGCCTCCCAACAACCAGAGCCCTCCGCGTTTCCCACAG CTGATGTACAGTCTGGAAGTCAGCGAAGCCATGAGGATCGGCGCGGTTTTATTAAATCTACAGGCAACTG ATCGAGAGGGAGACCCAATCACATATGCCATCGAGAATGGAGACCCTCAGCGAGTTTTTAATCTTTCAGA AACCACAGGGATTCTCAGCCTAGGGAAGGCTCTAGATCGAGAGAGCACGGACCGCTACATCCTCATCGTC ACAGCCTCAGATGGCAGGCCAGATGGAACCTCAACTGCCACTGTGAACATAGTGGTGACAGACGTCAATG ATAACGCTCCCGTGTTCGATCCCTATCTGCCCAGGAACCTCTCTGTGGTGGAGGAAGAGGCCAATGCCTT TGTGGGTCAAGTCCGGGCGACAGACCCAGATGCTGGGATAAATGGCCAAGTGCACTACAGCCTGGGGAAT TTCAACAACCTCTTCCGCATCACGTCCAACGGGAGCATTTACACAGCCGTGAAGCTGAACAGGGAAGCCA GGGACCACTATGAACTGGTTGTTGTGGCAACAGATGGAGCTGTACACCCTCGACATTCAACTCTGACACT GTACATCAAGGTGTTGGACATTGACGATAACAGCCCTGTTTTTACCAATTCAACGTACACGGTTGTTGTT GAAGAGAACCTGCCAGCCGGGACCGCCTTCCTTCAAATAGAGGCCAAGGATGTTGATCTTGGAGCCAATG TGTCCTATCGGATCAGAAGCCCGGAAGTGAAGCACCTCTTTGCACTGCATCCATTTACTGGGGAATTGTC TCTTCTGAGGAGTTTGGATTATGAGGCCTTTCCCGACCAAGAGGCAAGCATCACGTTCTTGGTGGAGGCC TTTGATATTTATGGGACGATGCCACCCGGTATAGCAACGGTCACAGTAATTGTGAAGGACATGAATGACT ACCCTCCGGTGTTTAGCAAACGCATCTACAAGGGGATGGTGGCTCCAGATGCCGTCAAGGGGACACCAAT TACCACTGTTTATGCTGAAGACGCAGACCCACCAGGGTTGCCAGCAAGTCGGGTGAGGTACCGAGTGGAT GATGTGCAGTTTCCATACCCGGCCAGTATTTTTGACGTAGAGGAAGATTCTGGAAGAGTAATAACCCGTG TTAATCTTAATGAAGAGCCTACAACAATTTTTAAGCTGGTGGTTGTGGCTTTTGATGATGGTGAACCTGT GATGTCCAGCAGTGCCACTGTGAGAATTCTTGTCTTACATCCTGGAGAGATCCCACGCTTCACCCAAGAG GAATACAGACCTCCTCCTGTGAGCGAGCTTGCTGCCAGAGGGACTGTAGTAGGCGTCATTTCTGCTGCCG CCATCAATCAGAGCATCGTGTACTCCATTGTGGCAGGAAATGAGGACGATAAATTTGGAATCAACAATGT CACTGGCGTCATCTATGTGAATTCCCCACTGGATTACGAGACAAGGACTAGCTATGTGCTCCGGGTGCAA GCAGATTCTCTGGAAGTGGTCCTCGCCAATCTCCGAGTCCCTTCAAAAAGCAATACAGCTAAGGTGTACA TTGAGATTCAGGATGAAAACGATCACCCCCCAGTGTTCCAGAAGAAATTCTACATTGGAGGTGTGTCTGA AGACGCGAGGATGTTCACGTCTGTGCTCAGAGTGAAGGCCACTGACAGGGACACAGGAAATTATAGCGCC ATGGCCTACAGACTCATCATACCACCGATTAAAGAGGGCAAAGAGGGGTTTGTGGTGGAAACATACACGG GGCTCATCAAGACAGCCATGCTCTTCCACAATATGAGAAGATCCTACTTCAAGTTTCAAGTGATTGCAAC CGACGACTACGGGAAGGGGTTGAGCGGGAAAGCGGACGTACTGGTCTCCGTGGTCAATCAACTGGATATG CAGGTCATTGTCTCCAATGTGCCCCCTACATTAGTGGAAAAGAAGATAGAAGATCTTACAGAGATTTTGG ATCGCTATGTTCAGGAGCAAATTCCTGGTGCCAAAGTTGTTGTAGAGTCCATTGGTGCCCGGCGCCATGG AGACGCCTTCTCCCTAGAAGACTATAGCAAGTGTGACCTGACCGTCTATGCCATCGACCCTCAGACCAAC AGAGCCATCGACAGAAATGAGCTTTTTAAGTTCCTGGACGGCAAACTGCTTGATATCAATAAAGACTTCC AGCCGTATTACGGGGAAGGAGGACGCATCCTGGAGATTCGGACTCCCGAAGCAGTGACGAGCATCAAGAA GCGAGGAGAGAGCTTGGGGTACACAGAAGGGGCCTTGCTGGCCTTGGCCTTCATCATTATCCTCTGCTGC ATCCCAGCCATCTTGGTCGTCTTAGTGAGCTACCGACAATTTAAAGTACGCCAGGCTGAGTGCACGAAGA CTGCAAGGATTCAGTCTGCTATGCCTGCAGCCAAGCCTGCAGCTCCTGTACCTGCTGCGCCCCCACCACC GCCGCCGCCACCACCACCAGGAGCACATCTCTATGAAGAACTGGGAGAGAGCGCCATGCATAAGTATGAG ATGCCCCAGTATGGAAGTCGCCGCCGACTGCTGCCACCTGCTGGACAGGAGGAATACGGCGAAGTCATTG GTGAAGCTGAAGAGGAGTATGAAGAGGAAGAGGTAAGTCCAGAGAAAGTTAAAAAGCCCAAAGTTGAAAT TAGAGAGCCTAGTGAGGAAGAGGTGGTAGTCACCGTTGAGAAGCCACCAGCGGCTGAGCCCACCTACCCG ACGTGGAAGAGAGCCAGGATCTTCCCAATGATCTTTAAGAAAGTCAGGGGTCTCGCTGAGAAAAGAGGCA TTGACCTTGAGGGCGAGGAGTGGAGGAGGCGCCTTGAGGAAGAAGACAAGGACTATCTCCAACTGACTCT AGACCAGGAGGAAGCCACGGAGAGCACCGTGGAGTCAGAGGAGGAGTCCAGCGACTACACGGAATACACA GAAACCGAGTCTGAGTTCAGCGAGTCCGAGACCACTGAAGAATCCGAGTCGGAGACCCCATCTGAGGAGG CAGAGGAAAGCTCGACCCCGGAGTCAGAGGAGTCTGAGTCCACCGAGTCAGAGGGAGAGAAGGCAAGGAA AAACATCGTGCTAGCAAGAAGAAGACCCGTGGTAGAAGAAATCCAAGAAGTGAAGGGTAAGAAAGAGGAG CCGCCTGTAGAGCAGGAAGAAGAGCCCCCGGGGGAGGAGGAAGAACGGCCAGAGGAAGGAGAAGAAAGCG AAGCAGCTCCCATGGATGAATCCACAGACCTGGAGGCTCAGGATGTCCCAGAGGAGGGCAGCGCAGAATC AGCCTCGGTGGAGAGGGGCGTGGAAAGTGAGGAGGAATCGGAGTCAGAAGTGAGCAGTAGCAGCAGCAGC AGCAGCAGTGAGAGCCTCTCTGGAGGCCCCTGGGGGTTCCAGGTACCAGAGTATGACAGGAGGAAGGAGG AAGATGCCAAGAAATCTCCAGGCGCAAACTCCGAAGGATACAACACAGCCCTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271377
Insert Size	5376 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271377.1, NP_001258306.1
RefSeq Size	5376 bp
RefSeq ORF	5376 bp
Locus ID	690865

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