Parp14 (NM_001191659) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1310490
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217691 representing NM_001191659 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGTCGGGTCCCTTCCCGCTGCTGGTCGAGGGCTCTTGGGGTCCCGACCCCCCGAAGAACTTGA TCAACAAGTTGCAAATGTACTTCCAGAGCCGGAAGAAATCTGGAGGAGGGGAGTGCGAGGTCGTCCCGGA GCCCGGGAACCCAGCACGCTTCCTGGTGCTCTTCTCCCCGGAGGATGTTCGGCAGAATGTTCTGGAGAAA GAGAATCATGAGTTAGTGTGGCAAGGAAAAGGAACATTCAAAATGACCGTCCAGTTGCCCGCAGACCCAG GGCAAGCCTCAGTCTCTAAGAAGGCAATTCCAGAAGAGGAGTCCAAGACAAAAGAAGATGCTGTGAAACC AGGTGATTTGGACACAACACCTTCTCCCAGCAGCAGATCAGAAGAAATGGAAGATGTCCCAAAAGAATGT GAAAATATTTCCTCCATGGTGGCATTTGAACAACTGCCCGAGAAAGTATCGGAGATGGTGCTGACCTTCC TGGTGGAGAATATAAGTGGCCTCCCTAGTGATGATTTTAAGGTGGAATTGATCCGAGATTTCGCTGTTGC TGTGGTTACCTTTCAGAAGCCTATAGATACCAAAAAATTTATTATTGATTGTATCAGCCACCGCTCAAAT CAACAACTCCAACTTGTCCCGAGACTTCTGGAAACGACCAATGTAGTGAGGGTGGAGAACCTGCCTCCTG GGGTGGATGATTACCAGCTGCAACTCTTCTTTGAAAACCCTTTCAACGGGGGTGGTAGGGTCGCCCGAGT TGAGTGTTTCCCCGAAGAGAGTTCGGCGCTGGTTGAGTTTTGTGACAGCAAAGTGTTAGACACCATTATG GCCAAGAAACACAGTTTCAACAAGATGCCGCTGTCTGTCTTCCCATATTACCCCTCCCTGGGCACAGCCT TGTATGGTGAGGAGAAGCCTCTGGTCAAGCTTCCAGCATCTTTCCAGGAGTCCCTGGATCTTCCTTTGTG GAAGTTCTTTCAGAAAAATGATCATCTGATGGAGGAGATAAATGATGAAATGAGGCACTGTCACTGCGAG CTGACGTGGTCTGAAATCAACGGTAAACTGACCGTCAGACCGGCCGCCACCTTAGTCAGTCACAGACCGA GCATCAAGACCTGGCAGAGGGATGCGTCCGCAGCACTCTCTGGCATCAGGTCTAAATATGATGTGATGGT GTTTGAAGTGTGCTCGCCAGTATGGGACATCATAAAGCATGAGTTAGGAGATGACAGGGTTTTGATTGAG TTTGAGAAGGAGAGTCTGAATGTAGCAGGAAAATCAGAAGATGTCCAAGGCATTAGCCAGCAAATCAGGG AACTAATAGAGCGCACCACTGAAAAGCTCAGAAGGGAAGAGCAGAGTCTGGAGGAGAAAGTGGCCATTTC CTCGGGAAAGCATTTTCTTCTGTACCACAGTGGCTTCCTGAAGGATCTAAGCAAAGAGTACCCGGAGATG GAGATCTCTTACGATGCCACGGCTCAGTACCTGTGCATTAAAGGCCTCCGTGCAGATGTGTACAAAGTAA AGTGTGAAATCCAAGAAAAGGTGTTTTCCATGGCTCAGAAAAATGTCCCGGTTTCTTCCGAAGTCTTCAG ATTTCTGCAGCAAGTAGACGGTCAGAGACTCTCCAATGCTCTGTTCAAGGCTCAGAACATTCTTGCCACA TACGAGCTGAAAGATAACGCTCTTTTCTTAATTGGAAGTTCTCCCAAAGATCTGGCAGAAGCTGAGGCCA AAATGCTCAGTGCTTTAAGTCATAAGCACATTGAAGTTGAGGACAAGGAAGTTCTGAGAAGTAATGTGTG GAAGAAGAAAATTCACCCTCTGCAAAAGAGACACGGCTCCTACGCAGCCATTATGGTCAAGAATGAGTTG ACGTCGGGAGTCCCGGCTGAGGTCGTCATCGCCGGCTGTGTGAAAGAAGTCAATGAAATTCATAGGCAGC TTTTCGAGTTTTTGGAAAACCACATGAAAGTTGAAAGAGTGGTCAAAATCGATCCACCCTTGATTATTGA CTATTTAAAGACAGACAAGAGGCTACTGTCAAAGACAAAGAAGGCAAATGTGAGTGTGAGTTTCAAGACT AAAGATGATCCAAACAGCATTTTGTTACTTGGCTCCAAGAGCAAAGTTCTAGAGTGCCTGGATACAGTCA AGGAAATCCGGGATTCAGTCTGCGTCAGAAAATTCCAGACTGACAAAGCCGGTGCCAGGCACTTCTTCCA GGATAAAGAATTCTATTACAAAATGGAGATCAGACGTCTGTATGGATGCATCGTTGAACTGAAGGAGGGT GAAGAGGGGGAGGAAGACAGCCTTAAGGAGCAGAAGTGCTACTTACAGAGGGACATAGGCCCTGGGGTTA CACTGATTGTCCACGAGGGAGACCTGAGTCTGTTTCCTGTTGATGTGGTAGTGAACGCAGCCAATGAGAG CCTTAAGCACATCGATGGCCTCGCGGGCACTCTCTCAAAGGCTGCGGGCCCCAGCCTCCAAGCAGAATGT GACCTGATCGTGAAAAAGGGAGGCATGGTCCCACCCGGCAATGCTGTTATCTCAAAAGCAGGCAAGCTCC CTTGTCGTTATGTCATCCATGCCGTAGGGCCTCGATGGAAGGGAGATAAGGTCCTGGAGTGTGTGAACCT CCTGAAGAAAGCTGTGAAACAAAGTCTCACTTTAGCTGAAGAGTACAAGTGCCAGTCCATAGCCATACCA GCGATCAGCTCTGGGATCTTTGATTTTCCCTTAGACCTGTGCGTGGCATCCATTGCTTCAGCCATCAAGG ACAACGTCCAACAGAAGCAGAACACGCACTCCGTGAAAAAGATTTATCTGGTGGACGTATCTGCAAAGGT TGCCAAGGCCTTTGCTGAAGCTGTCAAGACTACATATAGAGATATCCTTTCTCCAGCCAGTGTTAAAGCA TTAGTACCACCTGGGAAAATGCCCCAGAAGCAAGGCAAGCTGCTGGTGTCACCAGAAGGCTTGAACATCC GCCTGATTGAAGAAGGTGTGCAGAATGCTGAGGCCCATGCCATTGTGAACTCTGTTTCCTCGGATCTTAT ACTCAACAAAGGACCCCTTTCTCAGGCTTTCTTGGAAAGTGCTGGGCCAGAACTCCAGGAGGAATTGACC AAGGCAGGACAAGGGGTCTCTGTCAGTGTGGGTACAGTTCTTCAAACCAGCGGCTGTAATCTGAACAGCC GCTCCATCTTCCACGTGGTGACCCCACCATGGAAAAGCAACAACAGCGAGTGGTCACTAAAGATCATGAA AAACATCATCCGTGACTGTCTGAGGACCACCGAGGACCTGTCTCTACAATCCATTGCATTTCCAGCAATA GGAACGGGAAATCTGGGGTTTCCTAAACCCGAATTTGCTAAATTGATAATTTCAGAAGTACTCAAATTCA GTAGCAGGAACCAACTGAAGACTTTACAAGAGGTTCAGTTTCTGCTGCACCCCAAGGACCATGAAAACAT TCAGGCATTTTTAGATGAATTTGACAAAAGGAGTAATGGAGATCCTAGTAGCAAGAATCCCAAGGCTGAA GACACACAAGGTATCTATGGGAGTCTTTCCAGCCCTACTTTAGGAGTGCATGAGATGAATATTGGCCCGA TCCTCTTCCAAGTGGCCACCGGGGACATCATCAAAGAAGTGGCAGATGTGATTGTCAATTCCACATCATG CACGTTTAATCTCAAATCAGGGGTCTCCAAAGCAATTTTGGAAGGTGCAGGACAAAATGTGGAGCAAGAG TGTTCTCGCTTGGCTCAACAGGGTAAAGAGTACATAGTCACTGGAGGTGGATTATTGAAATGTAAGAGTA TTATTCATGTTGATGGTGGGAATGATGTCAAGAGATCAGTTTCCTGTATTTTGGAAGAATGTGAACAACG CAGTTTCTCATCCATTTGTCTACCAGCTATTGGCACAGGAAGCGCTCAACAGGATCCAAAGGTAGTAGCA AAAGCGGTATTGGATGCCATTGAAGAATTTGTCCAGAAGAAATCAGTGCAGGCTGTGAAGAGAGTTAAAG TTGTTATCTTCCAGCCCCATATTCTGCAATTTTTCTATGACAACATGAAAGAAAGAGAAGGTTCTCCAGC TCCTGTCAAGCCGTCAGTGATGTCTAAGATTGCCTCCTTCCTTGGCTTTCCAACACAAGCTTCACCCCCA AAGAACACCCTGGTTTTGGAAAAGAAAATTGAGCTCACAGTTTTCCAGATATGTGGCCCAGGTGTAGACA GTGTGGAAGGTACAATCTCCTGGCTAAAGAGTCTGATTACCAAAGAGCAGTTTTCCTTCACCAATGAGGA CGAGTGTGTCAGAGACTTTGACACCGAGGAGTACAGGAAACTGAATGAGATGCAGAAGAGGCTGAACATC ATCATTGAGCTGAACCAGAAGAAACCTTTGATTAAAGTCTCAGGAATCAGCAGAGACGTGGTAGAAGCTA GAGATGAGATTGACAACATGGTCAAGAGCATTAGATTGGCTAAGGAAAAGGAAAACCAGGCAGACTACGT CTCTACATTTGTGGAATGGCAATATATTGCTAATAACACCACACGGTGTTTTGACAAGATAGCCAACATG CAGTTGGAGGATGCATGGAAGGCAAAGAAGAAGCACACTGTTGTCAAAATTCAGAATCAGGACTTCACGG TGGACTTGAGCACAAACACAGCCACGGCTCCCCAAGGACTGAGTTTCACTGTCCAGCGACTCCTCAAAGC TGAAGCTGAAATTCCTGCCAATTGGAGTGATATGAAGCAGAACAAATTGCTTGTGGTGAGTCTGCAAACC AGTGATGCCGAGTACACCATGGTATCCAGGGCCTTCCATCAGACCTGCCCAAACTTTGTCATAGAGAAGA TTGAAAGGATTCAAAATCCAGCTCTTTGGAGGAGGTACCAGGCGTACAAAAAAATTATGGATGAAAAGAA TGGCAATGTGATAAATGAGAAGCAACTCTTCCATGGGACGGAGTTTGGCTCTCTGGCACAGCTCAACAGT AATGGATTTAACCGCAGCTATGCTGGGAAGAACGCTACAGCCTATGGGAAAGGAACCTATTTTGCAGTCA ATGCTTCCTATTCTGCCCATGACACATATTCCAAACCAGATGCCAATGGGAAAAAGTATATGTATTATGT GCGGGTCCTCACTGGAAACTACACACAAGGAAACGCATCACTGATTGTGCCTCCTTCAAGGGACCCTCAA AATGCTACTGACTTGTTTGACACTGTCGCAGATAATGTTATAAATCCGAGTATATTTGTAGTGTTTTATG ACAATCAAGCATACCCTGAGTATCTTATCACATTTAGGAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191659
Insert Size	5364 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191659.1, NP_001178588.1
RefSeq Size	7087 bp
RefSeq ORF	5364 bp
Locus ID	303903

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