Col16a1 (NM_001302967) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217662 representing NM_001302967 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGTATCCTGGGCTCCCGGCCTATGGGTGCTTGGGCTCTGGGCCACCTTCAGCCATGGAACAAATA TAGGTGAGCGCTGCCCAACTTCACAGGAAGAAGGACTCAAGCTGGAACACAGCAGTGACCCGTCAACCAA CATGACTGGCTTCAACCTAATCCGCAGGCTGAACCTTATGAAGACATCCGCCATCAAAAAGATCCGCAAC CCCAAGGGGCCTCTCATCTTGCGACTGGGGGCTGCCCCACTGACCCAAGCAACTCGACGAGTGTTCCCTC GGGGCCTCCCCGAGGAGTTTGCCCTGGTCCTGACGGTCCTTCTGAAGAAACACACCTTCCGGAATACATG GTACTTATTCCAAGTGACTGACGCAAATGGGTACCCACAGGTCTCCTTGGAAGTCAACAGCCAGGAGCGG ACTCTAGAGCTCCGGGCACAGGGCCAAGATGGTGACTTTGTGTCCTGCATCTTCCCAGTGCCTCAGCTCT TCGATCTGCGTTGGCACAAGCTGATGCTGAGCGTGGCAAGCCGTGTGGCCTCTGTGCATGTGGACTGCGT CTCGGCTTCCTCCCAGCCTCTGGGGCCCCGGCAGCCCATCCGGCCTGGGGGACATGTGTTTCTGGGCTTG GACGCTGAGCAGGGCAAGCCGGTTTCATTTGACCTTCAGCAGGCGCACATCTACTGTGACCCGGAGCTGG TGCTGGAGGAAGGCTGCTGTGAGATCTTACCGGGAGGGTGTCCCCCAGAGACCTCCAAGTCACGCCGGGA CACCCAGAGTAATGAGCTCATTGAGATCAATCCTCAGACCGAGGGCAAGGTCTACACCCGCTGCTTCTGC TTGGAAGAACCTCATAACAGCAAGGTGGACGCACAGCTGATGGGAAGAAGCAGCCAGAAGGCAGAAAGGG GGACAAAGGCCCACCAGGAGACTGGAGCCAGCGAGTGTCCGCCCTGTGCCCGCAGCGCCCGGGAGAGCAA TGTCACACTTGGTCCCCCTGGCCTCAAGGGAGGGAAAGGTGACCAAGGCCTGACTGGACCATCAGGCCCC AAGGGAGAGAAGGGAGCACGGGGCAATGACTGTGTTCGAATCTCCCCAGATGGCCCACTTCAGTGTGCAG AAGGTCCGAAAGGAGAGAAGGGAGAATCAGGAGACTTGGGACCCTCAGGACTCCCGGGCCCAACAGGCCA GAAGGGCCAGAAAGGGGAGAAGGGCGATGGAGGCCTCAAAGGACTGCCGGGAAAGCCGGGGCGAGATGGT CGGCCAGGAGAGATCTGTGTCATTGGGCCCAAAGGGCAGAAAGGAGACCCTGGCTTTGTTGGGCCTGAGG GATTGGCAGGAGAGCCTGGGCCCCCTGGCCTACCCGGACCACCTGGGATAGGACTGCCTGGGACTCCTGG AGATCCAGGTGGCCCACCAGGCCCTAAAGGAGCAAAGGGCAGCTCTGGAATACCAGGAAAGGAAGGCCCA GGAGGGAAGCCGGGGAAGCCAGGAGTACCCGGGACTAAGGGAGAAAAGGGTGACCCCTGTGAAGTGTGTC CAACGCTGCCCGAAGGGTTCCAGAACTTTGTGGGGCTCCCTGGAAAGCCAGGGCCTAAAGGAGAACCAGG TGATCCTGCTCCAGCCAGGGAGGGCCTAGGAACTGTTGGACTGAAAGGTGACCGGGGAGATCCAGGCATC CAAGGAATGAAAGGAGAGAAGGGGGAACCCTGCTCATCCTGCAGTTCAGGTGTTGGGGCCCAGCATCTTG GGCCCTCCACTGGGGTCAATGAAGACATGGGCTCCCCTGTCCATGGTTTACCTGGCCTTCCGGGCAAACC TGGAATTCCCGGGCCAAGAGGCATGAAAGGAGAGAAGGGTAGTTTTGGCGATGCTGGGCCGGCTGGAGTT GCGGGACCACCAGGACCAGTGGGACCGGCTGGTATCAAAGGGGCAAAGGGGGAGCCGTGTGAGCCCTGTG CATCTTTGTCCAAGCTCCAAGATGGGGACACTCAAGTGGTTCACCTGCCCGGCCCAGCTGGAGAGAAGGG AGAGCCTGGGCCCCCGGGCTCCGGGCTGCCTGGGAAGCAGGGTAGAGCTGGAGAGCGTGGACTCAAGGGG CAGAAGGGGGATGCTGGGAATCCAGGAGACCCTGGAACGCCAGGCATCACAGGGCAGCCTGGAATGTCTG GAGAGCCTGGGGTTCGGGGTCCTGCAGGACCAAAAGGAGAAAAGGGCGATGGATGCACTGCCTGCCCTAG CCTGCAGGGGGCGTTGACAGATGTATCGGGACTGCCTGGGAAACCAGGTCCCAAAGGAGAGCCAGGTCCT GAAGGTGTGGGTCGGCCCGGTAAACCAGGCCAGCCTGGTCTACCAGGAGTTCAAGGGCCTCCAGGACTGA AGGGCACTCAGGGAGAGCCTGGACCTCCAGGCACCGGTGCTGAGGGACCCCAGGGGGAGCCTGGAACCCC AGGTTTGCCTGGCATTCAGGGGCCCCCCGGACCTCGGGGACCACCTGGCTCAACCGGCGAACACGGTGCC CAGGGACCTCCAGGGCCCAAAGGAGCCACAGGACCCATGGGACCTCCTGGTGCTAGTATCTCTGGGCCTC CGGGCCAAAAGGGATCACGAGGAGAGAAAGGTGAACCGGGAGAGTGTTCCTGTCCTTCTCGAGGAGAGCC CATCTTCTCCGGCATGCCGGGTGCTCCGGGACTGTGGATGGGCAGCTCCTCGCAGCCGGGCCCGCAGGGT CCCCCAGGTGTCCCCGGACCACCAGGTCCCCCCGGAATGCCTGGATTGCAGGGAGTGCCTGGACACAACG GTTTGCCAGGACAGCCTGGGCTCACTGCCGAGCTGGGATCCTTACCCATCGAGCAGCATCTCCTCAAGAG CATCTGTGGTGACTGTGCCCAGGGCCAGGCAGCCCATTCAGCCTCCCTTCTGGTGAAAGGAGAAAAGGGG GACCAGGGTGTCCCTGGTGTGCCGGGTTTCAACGACTGTGCCAGGTGCTTCATAGAGAGGGAGCGCCCGA GAGCTGAGGAGGCCAGGGGAGACAACAGTGAGGGAGAACCTGGCTGTCCTGGGAGCCCTGGCCTGCCTGG TCCTCCAGGGATGCCTGGCCAAAGAGGAGAAGAGGGTCCACCTGGCATGAGGGGCTCTCCTGGTCTTCCA GGCCCTGTTGGTCTTCAAGGGGAGCGAGGCCCCACAGGCCTGACTGGAGACAAGGGAGAACCGGGTCCCC CAGGACAGCCTGGCTACCCGGGTGCCATGGGTCCCCCAGGACTGCCTGGCATCAAGGGAGAAAGAGGATA TGCTGGCCCTGCGGGAGAGAAGGGGGAGTCGGGCCCACCAGGATCTGAAGGCCTTCCAGGTCCCCAAGGT CCAGCGGGTCCCCGAGGAGAGCGAGGTCCCCAAGGGAGCTCGGGAGAGAAGGGTGATCAGGGATTTCAAG GCCAGCCAGGTTTCCCTGGCCCACCGGGTCCCCCGGGATTCCCAGGCAAAGCTGGAGCTCCTGGCCCACC TGGCCCCCAAGCAGAGAAGGGCAGTGAAGGGATGCGAGGTCCCTCAGGCTTGCCTGGTTCCCCTGGACCA CCAGGCCCTCCTGGGATTCAGGGCCCTGCTGGACTGGATGGACTGGATGGGAAAGACGGCAAACCGGGCC TGAGGGGGGACCCAGGTCCCGCTGGCCCTCCTGGACTCATGGGACCTCCAGGTTTCAAAGGGAAAACAGG GCATCCTGGTCTCCCTGGACCTAAGGGTGACTGTGGAAAACCAGGTCCTCCTGGCAGCAGTGGCCGGCCA GGTGCAGAGGGTGAGCCCGGTGCCATGGGACCCCAGGGACGACCAGGACCTCCAGGGCATCTTGGGCCAC AGGGGCCTCCAGGCCAGCCAGGTCCACCTGGGCTCTCCACCATGGGCCTGAAAGGAGATCGGGGATCCCC TGGAGAAAGGGGCTTGGCCGGCCTCCCAGGCCAGCCTGGCACACCCGGACACCCCGGCCCTCCGGGTGAA CCTGGTTCGGATGGTGCAGCTGGCAAAGAGGGACCTCCAGGAAAGCAGGGACTCTATGGACCCCCCGGCC CAAAGGGTGACCCAGGACCTGCAGGACAGAAAGGCCAAGCAGGAGAAAAGGGAAGATCTGGCATGCCTGG GGGCCCTGGCAAGAGTGGCTCCATGGGACCGATTGGACCACCGGGTCCTGCGGGAGAGAGAGGCCACCCT GGGTCCCCAGGGCCTGCTGGGAACCCTGGATTGCCTGGTTTGCCAGGCTCTATGGGAGACATGGTGAATT ATGATGATATCAAGAGGTTTATCAGACAAGAGATCATTAAACTGTTTGATGAGAGGATGGCTTACTACAC CTCTAGGATGCAGTTCCCCATGGAGGTGGCAGCAGCTCCAGGCCGGCCAGGGCCTCCAGGGAAGGATGGC GCCCCAGGGCGGCCAGGTGCTCCAGGGTCACCTGGGCTCCCTGGTCAGATAGGCCGAGAAGGACGCCAGG GTTTGCCAGGAATGAGAGGATTGCCTGGTACCAAAGGTGATAAGGGAGACATTGGTGTTGGCATTGCAGG AGAAAATGGTCTCCCTGGCCCTCCAGGTCCTCAAGGGCCTCCAGGTTATGGCAAGATGGGTGCAACCGGG CCAATGGGCCAGCAAGGCATTCCTGGGATCCCTGGCCCCCCGGGGCCCATGGGCCAGCCAGGGAAGGCAG GCCACTGCAACCCCTCAGACTGTTTTGGGGCCATGCCAATGGAGCAGCAGTACCCGCCCATAAAAAGCAT GAAGGGGCCTTTTGGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302967
Insert Size	4779 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302967.1, NP_001289896.1
RefSeq Size	5212 bp
RefSeq ORF	4779 bp
Locus ID	366474

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