Sik3 (NM_001271216) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217601 representing NM_001271216 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGTTGCCGTGGCCGGAGCAGGGGGAGCCGGGCCGGCCG GTCGCCTGCTGCCTCCGCCCGCCGCGGGGCCCCCGGCAGCCCCCGCCGCAGTGCCCCCGGCCGCCTGCCC GCCGCGTCCCACAGCCCCGGCCTCCCGCGGGTCGATGGCCGCTCGCATCGGCTACTACGAGATCGACCGC ACCATCGGCAAGGGCAACTTCGCTGTGGTCAAGCGGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTA TTAAAATCATAGATAAGACCCAGTTGGATGAAGAGAACCTGAAGAAGATTTTCCGGGAGGTTCAGATAAT GAAGATGCTTTGCCACCCACATATCATCAGGCTCTACCAGGTCATGGAGACAGAGCGCATGATTTACCTG GTGACAGAATACGCTAGCGGAGGGGAGATATTTGACCACTTGGTGGCCCATGGAAGAATGGCGGAGAAGG AAGCTCGGCGGAAGTTCAAACAGATTGTCACAGCTGTATATTTTTGTCACTGTCGGAATATCGTTCATCG TGATTTAAAAGCTGAAAATTTGCTTCTGGACGCCAATCTGAATATCAAAATAGCAGACTTTGGCTTCAGC AACCTCTTCACTCCGGGTCAGCTGCTAAAGACGTGGTGTGGCAGTCCTCCCTATGCCGCACCAGAGCTCT TCGAAGGCAAGGAATATGATGGGCCCAAAGTGGACATATGGAGTCTTGGAGTTGTCCTCTATGTGCTTGT GTGTGGCGCCCTGCCGTTTGATGGGAGCACACTGCAGAATCTGCGGGCCCGTGTGCTGAGCGGCAAGTTC CGCATCCCGTTCTTCATGTCCACAGAGTGTGAGCACTTGATCCGCCACATGCTGGTGTTAGATCCAAATA AGCGTCTCTCAATGGAGCAGATCTGCAGGCACAGGTGGATGAAGCTCGGAGATGCAGACCCCAACTTTGA GAGGTTAATAGCAGAATGCCAGCAGCTGAAGGAGGAAAGGCAGGCAGAGCCCCTCAATGATGAGGTTCTC TTGGCCATGGAAGACATGGGACTAGACAAGGAACGCACACTTCAGTCATTAAGATCGGATGCCTATGACC ACTATAGTGCGATCTACAGCCTGCTGTGCGATCGACACAAGAAAAACAAAACTCTGCGTCCTGGAGCACT TCCCAGCACGCCCCAAGCCATGACTTTTCAGGCACCAGTCAATATCCAGGCGGAGCAGACAGGCACTGCT ATGAACATTAGCGTCCCCCAAGTTCAGCTGATCAACCCAGAGAACCAAATTATAGAGCCTGACGGGGCCG TGAACTTGGACAGCGACGAGGGTGAAGAGCCTTCTCCAGAAGCCTTGGTTCGCTATTTGTCAATGAGGAG GCACACGGTGGGAGTGGCTGACCCACGCACGGAAGTTATGGACGAGATGCAGAAGCTCCTGCCCGGCTTC CCTGGAGCGAACCCACAGACTCCATTCCTGCAGGTGGCCCCTAACATGAACTTCATGCACAACCTGCTGC CCATGCAGAGTCTGCAGCCAACTGGGCAGCTTGAGTACAAGGAGCAGTCTCTATTACAGCCGCCTACACT ACAGCTACTGAATGGAATGGGGCCCCTTGGCCGAAGAGCCTCAGACGGAGGCGCCAACATCCAACTACAT GCCCAGCAGCTGCTCAAGCGCCCACGGGGACCATCCCCACTTGTCACCATGACACCAGCAGTGCCAGCAG TTACCCCTGTGGATGAGGAGAGCTCGGATGGGGAGCCAGATCAGGAAGCTGTGCAGAGGTACTTGGCAAA TAGGTCCAAAAGGCACACACTGGCCATGACCAGCCCGACAGCTGAGATCCCACCGGACCTGCAACGGCAG CTAGGACAACAGTCTTTCCGTTCCCGGGTCTGGCCTCCTCACCTGGTACCTGACCAGCATCGCTCCACCT ACAAGGACTCCAACACCCTGCATCTCCCTACGGAGCGTTTCTCCCCCGTGCGCCGGTTCTCAGACGGGGC GGCAAGCATCCAGGCCTTCAAAGCTCACCTGGAAAGAATGGGCAACAGCAGCAGCATCAAACAGTTACAA CAGGAGTGTGAGCAGCTACAGAAGATGTACGGCGGGCAGGTTGACGAGAGAACCCTGGAGAAGACCCAGC AGCAGCATATGCTGTACCAGCAGGAGCAGCACCATCAGATCCTCCAGCAGCAAATTCAAGATTCTATTTG TCCTCCTCAGCCCTCCCCACCTCTTCAGGCTGCATGTGAAAATCAGCCAACCCTCCTCACCCACCAGCTG CAGAGGTTAAGGATTCAACCTTCAAGCCCACCCCCCAACCACCCCAGCAACCATCTCTTCAGGCAGCCAA GTAACAGTCCTCCCCCAGTCAGCAGTGCCATGATCTCTTCTCACGGTGCTACGTCTCCCTCCCAGTTTCA AGGCTTACCCTCCCATGGTGCAATCTTCCAGCAGCAACCTGAGAACTGTTCCCCACCTCCCAGTGTAGCA CTAACCTGCTTGGGCCTACAGCAGGCTAGCCAGTCACAGCCAGTGACTATCCAGCTACAGGAGCCAGTTG ACATGCTCAGCAACATGGCTGGGACAACTGCAGGTTCTGCAGGGCGGGGTATCCCCATCAGCCCCAGTGC CAGTCAGATTCAGATGCAGCACCGTACCAGCCTAATGGCTCCCTTCGGCTATGGGCACCGGCCCTTGTCC AAGCAGCTGAGTGCTGACAGCGCAGAGGCTCACAGCTTGAGCATGAATCGATTCTCCCCTGCCAACTACG ACCAGGCGCATTTACACCCCCATCTGTTCTCGGAGCAGTCCCGAGGGCCCCCCAGCAGCTACAGCCCTTC AACAGGAGTGGGGTTTCCTCCGGCTCAAGCCCTGAGAGTTCCTCCGCTTGACCAGTTCCCCACTTTCCCT CCCAGCGCCCATCAGCAGCCACACTATACCACATCAGCACTACAGCAGGCCCTGCTGTCCCCTACACCAC CAGACTATACCAGACACCAGCAGGTTCCCCACATCCTTCAAGGACTGCTCTCTCCCCGGCATTCACTCAC CGGCCACTCGGACATTCGGCTGCCTCCAGCAGAGTTTGCCCAGCTCATCAAAAGACAGCAGCAGCATCGA CAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAAGAATACCATGAGCTGTTCAGGCACATGAACCAAG GGGATGCTGTTAGCCTAGCTCCCGGCCTCGGGGGACAGAATATGACAGAGCACCAGGCTTTATCTTATCA AAATGCTGACTCATACCACCGCCACCACACCAGCCCCCAGCATCTCCTACAGATCAGAGCGCAAGATTGT ATCTCACAGGGTCCCTTGCCCACCCCCACCCGTGGGTATGCCCGTCAGTCACCACTAATGCATTCGGAGA GTATGGAAGAAGACTGCTTGTGTGAAGGGGTCAAGGAGGGCTTCGCGGACAAAAGCTCAAGCACACTGAC CAAAGGTTGCCATGACAGCCCTCTGCTCTTGTGTACCAGTGGGCCTGGGGACCCCGAGCCTTTGCTGGGA ACTGTGAGTCAGGCCCGGGAACTGGGAATCCATCCCTACGGACACCAGCCAACGGCCACCACATTCAGTA GAAATAAGGTGCCCAGCCGAGAGTCTGTCATAGGGAACTGCATGGAGAGAAGTTCTCCTGGACAAGCAAT GGAGCTGCCGGACCACAACGGCCTTGGGTATCCTGCACGGCCCTTAGTCAGTGAGCACCTCAGGTCCCGG ACCCTCCAGAGACACCACACCATCCAGAACAGCGACGATGCTTATGTACAGCTGGATACCTTGCCAGGAA TGAGCCTGGTGGCAGGAAAGGCACTGAGCTCTGCCCGGATGTCAGATGCAGTTCTTAGTCAGTCTTCACT CATGGGCAGCCAGCAGTTTCAGGATGAGGAAGATGAAGAATGTGGGGTGAGCCTGGGCCATGAGCACCCA GCCCTGGGTGACGGTAGCCAGCATTTAAACTCCCGCTATCCAGCTACGTGTGTTACAGACATCATGCTCA ACCACAAGCATCCAGAGGTCTCCTTCAGCATGGAGCAGGCTGGCGTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271216
Insert Size	4110 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271216.2, NP_001258145.2
RefSeq Size	6248 bp
RefSeq ORF	4110 bp
Locus ID	684112

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