Irs2 (NM_001168633) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	4PS; IRS-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217591 representing NM_001168633 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAGCGCGCCCCTGCCTGGGCCCCCCGCGTCGGCGGGCGGGGACGGCCCGAACCTCAATAACAACA ACAACAACAACAACAACCACAGCGTGCGCAAGTGCGGCTACCTGCGCAAGCAGAAGCACGGCCACAAGCG CTTCTTCGTGCTGCGCGGCCCGGGCACCGGCGGCGAGGAGGCAGCCGCGGCTGGGGGGTCGCCGCCGCAG CCTCCGCGGCTGGAGTACTATGAGAGCGAGAAGAAGTGGAAGAGCAAGGCGGGCGCGCCGAAGCGAGTGA TCGCGCTCGACTGCTGTCTGAACATCAACAAGCGCGCGGACGCCAAGCACAAGTACCTGATCGCCCTCTA CACCAAGGACGAGTACTTCGCCGTAGCGGCGGAGAACGAGCAGGAGCAGGAGGGCTGGTACCGCGCACTC ACCGACTTGGTCAGCGAAGGCCGCTCTGGCGATGGGGGCTCCGGCACCACGGGCGGCTCTTGCAGCGCCT CTCTCCCGGGGGCCCTGGGCGGCTCGGCGGGCGCCGCTGGCTGCGATGACAACTACGGGCTCGTGACGCC CGCCACGGCAGTCTACCGCGAGGTGTGGCAGGTGAACCTGAAGCCTAAGGGACTGGGCCAGAGCAAGAAC CTGACCGGTGTATACCGCCTATGCCTGTCTGCGCGCACCATCGGCTTCGTGAAGCTCAATTGCGAACAGC CGTCGGTGACGCTGCAGCTTATGAACATTCGCCGCTGCGGCCACTCGGACAGCTTCTTCTTCATCGAGGT GGGCCGTTCAGCCGTCACCGGTCCCGGCGAGCTGTGGATGCAAGCCGATGACTCGGTGGTGGCGCAGAAC ATCCATGAGACCATCCTGGAGGCCATGAAGGCACTCAAGGAGCTCTTCGAGTTCCGGCCTCGCAGCAAGA GTCAGTCGTCTGGGTCGTCTGCCACGCACCCCATCAGCGTACCGGGCGCGCGCCGCCACCACCACCTAGT CAACCTACCCCCTAGCCAGACGGGCCTGGTGCGCCGCTCTCGCACTGATAGCCTGGCGGCCACCCCGCCA GCAGCCAAGTGCACTTCGTGCCGGGTTCGTACAGCCAGCGAGGGTGACGGCGGCGCGGCAGGCGGAGCCG GGACGGCAGGAGGCAGGCCGATGTCGGTGGCAGGGAGCCCCCTGAGTCCCGGGCCGGTGCGCGCGCCCCT TAGCCGCTCGCACACCCTGAGCGCCGGCTGCGGAGGCCGCCCGAGCAAAGTGGCTCTGGCGCCGGCAGGG GGAGCCCTACAACACAGCCGCTCCATGTCCATGCCCGTGGCGCACTCGCCGCCTGCAGCCACCAGCCCAG GCAGCCTGTCCTCCAGCAGTGGGCATGGCTCAGGCTCCTACCCGCTGCCTCCTGGTTCCCACCCGCACCT GCCTCATCCGCTCCATCACCCCCAATGCCAACGTCCGTCCAGCGGAAGTGCCTCCGCCTCGGGCTCCCCC AGCGACCCGGGTTTCATGTCCCTTGACGAGTATGGCTCCAGCCCTGGTGACCTGAGAGCCTTCAGTAGCC ACAGGAGCAACACACCTGAGTCCATCGCGGAGACCCCGCCAGCCAGGGACGGCAGTGGGGGCGAGCTCTA TGGGTATATGAGCATGGATAGGCCCCTGAGCCACTGTGGCCGCCCTTACCGTAGGGTCTCTGGGGATGGG GCCCAGGATCTGGACAGAGGACTGAGGAAGAGGACTTACTCCCTAACCACGCCTGCCCGGCAGCGGCAGG TTCCTCAGCCTTCCTCTGCCTCTCTAGACGAATACACTCTCATGCGGGCCACCTTCTCTGGCAGTTCAGG TCGCCTCTGCCCATCCCTCCCTGCGTCCTCTCCCAAAGTGGCCTACAACCCTTACCCAGAGGACTATGGA GACATTGAGATTGGTTCTCACAAGAGTTCCAGCAGTAACCTGGGGGCAGATGATGGCTACATGCCCATGA CCCCTGGGGCAGCCCTCAGGAGTGGTGGCCCCAATAGCTGCAAGAGCGATGACTACATGCCCATGAGCCC CACCAGCGTGTCTGCCCCTAAGCAGATCCTGCAACCACGTTCGGCAGCGGCCTTGCCCCCCTCTGGAGCA GCCGTGCCAGCACCCCCTTCAGGGGCGGGCAGGACTTTCCCAGTGAACGGAGGCGGCTACAAAGCCAGCT CCCCAGCGGAGAGCTCCCCAGAAGATAGCGGGTACATGCGAATGTGGTGTGGCTCCAAGCTGTCCATGGA GAACCCAGACCCTAAGCTGCTCCCCAATGGGGACTACCTCAACATGTCCCCCAGTGAGGCAGGCACCGCA GGGACCCCACCTGACTTCTTCTCAGCAGCTTTGCGTCCAGGCGGTGAGGCCCTCAAAGGCGTCCCTGGCC ACTGCTACAGCTCTTTGCCCCGCTCTTACAAGGCTCCCTGTACTTGCGGTGGTGGAGACAACGACCAGTA CGTGCTCATGAGCTCCCCTGTGGGTCGGATTTTGGAAGAGGAGAGACTGGAGCCCCAGGCCACCCCAGGG GCTGGCACATTTGGGGCAGCTGGTGGTAGTCATACCCAGCCTCATCACTCAGCAGTGCCTTCCTCCATGA GACCAAGTGGCATCGTTGGCCGACCTGAGGGCTTCCTGGGCCAGCGCTGTCGGGCAGTGCGGCCCACACG CCTTTCGCTAGAGGGACTGCAGACCCTTCCCAGCATGCAAGAGTACCCTCTACCCACTGAGCCCAAGAGC CCTGGCGAGTACATCAACATTGACTTTGGTGAAGGGGGTACCCGTCTGTCTCCGCCTGCTCCCCCACTAC TGGCATCAGCGGCCTCCTCCTCTTCACTGCTCTCAGCCAGTAGTCCTGCTTCATCCCTGGGTTCAGGTAC CCCAGGCACCAGCAGTGACAGCCGGCAGCGCTCTCCGCTCTCTGACTATATGAACCTGGACTTCAGTTCT CCCAAGTCCCCTAAGCCTAGCACCCGCAGTGGGGACACAGTGGGCTCCATCGATGGCCTTCTCTCTCCAG AAGCCTCATCCCCATACCCACCGCTGCCCCCGCGTCCTTCTGCTTCCCCTTCCTCCTTACAGCAGCCTCT GCCACCTGCCCCAGGAGACCTATACCGCCTGCCTCCAGCAACAGCTGCCACATCCCAGGGTCCCACTGCT GGCTCCTCAATGTCCTCTGAGCCTGGGGATAATGGTGACTATACCGAGATGGCCTTTGGTGTGGCTGCTA CCCCGCCACAACCTATTGCGGCACCCCCGAAGCCAGAAGGTGCCCGAGTGACCAGTCCCACATCAGGCTT GAAGCGGCTAAGTCTCATGGATCAGGTATCTGGGGTAGAGGCCTTCCTTCAAGTCAGCCAGCCCCCTGAT CCCCACCGGGGCGCTAAGGTCATCCGTGCAGACCCGCAGGGGGGACGTCGTCGCCACAGTTCAGAGACCT TCTCCTCTACCACCACTGTCACCCCAGTGTCCCCATCCTTTGCTCACAATTCCAAGCGCCACAATTCGGC CTCTGTGGAAAACGTCTCTCTCAGGAAAAGCAGTGAAGGCAACAGCATCCTGGGAGGAAGTGATGAGCCA TCCACATCCCCAGGACAGGCACAGCCCTCGGCAGGTGTGCCCCCAGCGCCACAGGCTAGGCCATGGAACC CCGGTCAGCCTGGAGCTTTGATTGGCTGTCCTGGAGGCAGCAGTTCTCCCATGCGCAGAGAGACCTCCGT GGGTTTCCAGAACGGCCTCAACTATATCGCCATCGATGTGAGAGGTGAGCAGGGGTCCTTGGCGCAGTCT CAGCCTCAGCATCCACAGCCAGGAGACAAGAACTCCTGGGGCCGGACCCGTAGCCTTGGGGGGCTCCTCG GTACCGTTGGAGGCTCTGGCACCAGTGGAGTGTGTGGGGGTCCAGGCACTGGAGCCTTACCCTCTGCCAG CACCTACGCAAGCATCGACTTCTTGTCCCATCACTTGAAAGAAGCCACCGTGGTGAAAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001168633
Insert Size	3984 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001168633.1, NP_001162104.1
RefSeq Size	6301 bp
RefSeq ORF	3984 bp
Locus ID	29376
Gene Summary	plays a role in insulin-stimulated fetal liver growth [RGD, Feb 2006]

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