Kdm2b (NM_001100679) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Fbxl10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217582 representing NM_001100679 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCAGAGAAAGACTCTGGAAGAAGATTGCGTGCGATTGACCGCCAGAGATACGACGAGAACGAAG ACTTGTCGGACGTGGAGGAAATTGTCAGCGTCCGTGGCTTCAGCCTGGAGGAGAAGCTACGTAGCCAGCT ATACCAGGGGGACTTCGTGCATGCCATGGAAGGCAAAGATTTTAACTATGAGTACGTACAGAGAGAAGCT CTCAGGGTTCCCCTGGTTTTTCGAGACAAGGATGGACTAGGAATCAAGATGCCAGACCCTGACTTCACAG TCCGAGACGTCAAACTCCTGGTGGGGAGTCGCCGGCTGGTGGACGTGATGGACGTGAACACCCAGAAGGG CACGGAGATGAGCATGTCCCAGTTCGTGCGTTACTACGAGACACCAGAGGCACAGAGGGACAAACTGTAC AATGTCATCAGCCTCGAGTTCAGCCATACGAAGCTGGAGCATCTAGTCAAGCGTCCCACTGTGGTGGACC TGGTCGACTGGGTGGACAACATGTGGCCCCAACATCTAAAGGAAAAGCAGACAGAAGCCACAAACGCCCT TGCCGAGATGAAATACCCCAAAGTGAAGAAGTACTGTCTGATGAGCGTGAAGGGCTGTTTCACCGACTTC CACATTGACTTTGGAGGCACCTCCGTGTGGTACCATGTTTTCCGTGGCGGGAAGATCTTTTGGCTGATCC CCCCAACCCTGCACAACTTGGCTCTGTATGAGGAGTGGGTGCTGTCTGGCAAACAGAGTGACATCTTTCT GGGAGACCGAGTGGAACGCTGCCAAAGAATTGAGCTGAAGCAAGGCTACACATTTTTCATCCCTTCTGGT TGGATCCATGCAGTGTACACGCCCGTGGACTCTCTGGTGTTCGGCGGGAACATCCTGCACAGCTTCAACG TGCCCATGCAGCTGCGCATCTACGAGATCGAGGACAGGACCCGGGTTCAGCCCAAGTTCCGTTACCCCTT CTACTATGAGATGTGCTGGTATGTCTTGGAGAGATACGTGTACTGTGTGACCCAGCGCTCCTACCTCACT CAGGAATACCAGCGAGAATTAATGCTCATTGATGCCCCAAGAAAAAACAGTGTAGACGGTTTCTCATCTG ATTCCTGGCTGGAGATGGAGGAGGAGTCCCGTGAGCAGCAGCTCCAGGAGGAGGAGGACAAGGAAGAGGA AGGGGATGGTGCAGACAAAACACCCAAGCCACCCACCGAGGGCCCCACCTCACCCACCAGCACCCAGTCG GAAGACCAGGACAGCACAGGGAAGAAGCCTAAAGCCCCTGCTATGCGATTCCTCAAGAGGACTTTGTCCA ATGAGTCCGAGGAAAGTGTCAAGTCCACAGCGATGCCCGTAGACTACCCCAAGACGCCCACAGGCTCCCC GGCCACTGAGGTTTCTACCAAGTGGACTCACCTTACCGAATTTGAACTGAAGGGCTTGAAAGCCCTGGTT GAAAAACTAGAGTCTCTTCCGGAGAATAAGAAGTGTGTCCCTGAGGGAATTGAGGACCCCCAGGCCCTCC TGGAAGGTGTTAAGAATGTACTGAAGGAACACGTGGATGATGACCCCAACCTGGCCATCACCGGGGTCCC CGTGGTCAGTTGGCCAAAGAAAACTCCAAAGAACCGGGTGGTGGGTCGGCCTAAGGGCAAGTTGGGCCCG GCCTCAGCGGTGAAGTTGGCTGCCAACCGAACAACAGCAGGAGCTCGCAGGCGCCGGACGCGATGCCGCA AGTGCGAGGCCTGCCTGCGGACGGAGTGTGGAGAGTGCCACTTTTGCAAGGACATGAAGAAGTTCGGAGG TCCTGGGCGCATGAAGCAGAGCTGCATCATGCGGCAGTGCATCGCGCCAGTGCTGCCCCACACCGCCGTG TGCCTTGTGTGTGGCGAGGCAGGGAAGGAGGACACAGTGGAAGAGGAAGAAGGCAAGTTTAACCTCATGC TCATGGAGTGCTCCATCTGCAACGAGATCATCCACCCTGGATGCCTTAAGATTAAGGAATCAGAGGGTGT GGTCAACGATGAGCTTCCCAACTGCTGGGAGTGTCCGAAGTGTAACCATGCCGGCAAGACCGGGAAACAA AAGCGTGGCCCTGGCTTTAAGTATGCCTCCAACTTGCCTGGCTCCTTGCTCAAGGAGCAGAAGATGAACC GGGACAACAAGGAAGGGCAGGAGCCTGCCAAGCGGAGGAGTGAGTGTGAAGAGGCCCCCCGTCGCAGGTC AGACGAGCACCCCAAGAAGGTGCCTACAGATGGCATCCTCCGCCGAAAGTCTGACGATGTGCACCTGAGG AGGAAGCGGAAATACGAGAAGCCCCAAGAGCTGAGTGCACGCAAGCGAGCCTCGACGCTTCAAACGTCCC CCGGTTCCTCCTCTCACCTCTCGCCGAGGCCCCCTCTAGGCAGCAGTCTCAGCCCTTGGTGGAGATCCAG TCTCACTTACTTCCAGCAGCAGCTAAAACCTGGCAAAGAAGATAAGCTTCTCAGGAAAAAGCGGCGGTCC TGGAAGAACGCTGAGGATCGGCTGTCACTGGCCAACAAGCCCCTTCGGCGCTTCAAGCAGGAGCCAGAGG ACGACCTGCCTGAGGCACCTCCTAAGACCCGGGAGAGTGATCAGTCACGGTCCAGCTCGCCCACAGCTGG GCCCAGCACTGAGGGGGCCGAGGGCCCAGAAGAGAAGAGGAAGGTGAAGATGCGCCGGAAACGGCGGCTT CCCAACAAGGAGTTGAGCAAAGAGCTAAGCAAGGAGCTCAACCACGAGATCCAAAAGACAGAGAGCACCT TAGCCCACGAGAACCACCAGCCTATCAAGTCAGAGCCCGAGAGTGAAAACGAGGAGCCAAAGAGGCCCTT AAGCCACTGCGAGCGCCCGCACCGCTTCAGCAAAGGGCTCAACGGCACTCCTCGGGAGCTGCGGCACTCA CTGGGACCTGGGCTTCGCAGCCCACCTCGTGTCATCTCCCGGCCCCCGCCCTCTACATCCCCACCCAAGT GCATCCAGATGGAGCGTCACGTGATCCGGCCACCACCCATCAGCCCCCCACCTGACTCGCTGCCCCTGGA TGATGGAGCAGCCCATGTCATGCATAGGGAGGTGTGGATGGCAGTCTTCAGCTACCTCAGCCACCAAGAC CTGTGTGTCTGCATGCGGGTCTGCAGGACCTGGAACCGCTGGTGCTGCGATAAGCGGTTGTGGACCCGCA TCGACCTGAACCACTGCAAGTCCATCACTCCCCTGATGCTGAGCGGCATCATCCGGCGGCAGCCGGTCTC CCTGGATCTCAGCTGGACCAACATCTCCAAGAAGCAGCTGAGCTGGCTCATCAACCGGTTGCCTGGGCTC CGAGACTTGGTGCTGTCAGGCTGCTCATGGATCGCTGTCTCAGCCCTCTGTAGCTCCAGTTGTCCACTGC TCCGGACCCTGGATGTCCAGTGGGTAGAAGGACTAAAGGATGCCCAGATGCGGGATCTCCTGTCTCCACC CACAGACAACAGGCCAGGTCAAATGGACAATCGGAGCAAGCTCCGGAACATTGTGGAACTGCGCCTAGCT GGCCTGGACATCACAGATGTCTCCCTGCGGCTCATTATTCGCCATATGCCCCTGCTCTCCAAGCTCCACC TCAGTTACTGTAACCACGTCACTGATCAGTCCATCAACCTGCTCACTGCGGTCGGCACCACCACCCGGGA CTCTCTGACAGAGATCAACCTATCGGACTGCAATAAGGTCACTGACCAGTGCCTGTCCTTCTTCAAACGC TGTGGAAATATCTGTCATATTGACCTGAGGTACTGCAAGCAAGTCACCAAGGAAGGCTGTGAGCAGTTCA TAGCTGAAATGTCTGTGAGTGTCCAGTTTGGGCAAGTGGAAGAGAAACTCCTGCAAAAACTAAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100679
Insert Size	3918 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100679.1, NP_001094149.1
RefSeq Size	5190 bp
RefSeq ORF	3918 bp
Locus ID	304495

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