Phka2 (NM_001190994) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217557 representing NM_001190994 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGAGCAGGAGCAATTCTGGGGTCCGCCTGGACGGGTACGCGAGGCTGGTGCAGCAAACCATCCTGT GTTACCAGAACCCAGTCACTGGGCTGTTATCAGCTAGCCATGACCAGAAGGATGCCTGGGTACGGGACAA CATCTATAGCATACTGGCTGTTTGGGGCCTGGGCATGGCGTACCGTAAGAATGCTGACCGTGATGAGGAT AAAGCCAAGGCCTATGAATTGGAACAGAACGTAGTGAAGCTGATGCGAGGTCTTCTCCAGTGTATGATGA GGCAGGTGGATAAAGTGGAGAAGTTCAAGCATACTCAGAGTACCAAGGACAGTCTACATGCCAAGTACAA CACTGCCACATGCAGCACTGTGGTGGGTGATGACCAGTGGGGCCACCTCCAGGTGGATGCCACCTCCCTC TTCCTCCTGTTCCTGGCCCAGATGACTGCCTCAGGTTTGCGTATTATTTTTACCCTCGACGAAGTGGCCT TCATACAGAATCTTGTCTTCTACATAGAGGCTGCATATAAAGTTGCTGATTATGGAATGTGGGAACGTGG AGATAAGACCAATCAGGGCATTCCAGAATTGAATGCAAGCTCAGTGGGAATGGCCAAGGCAGCACTCGAA GCAATTGATGAACTAGATCTTTTTGGAGCCCATGGAGGACGCAAATCGGTGATCCATGTCCTGCCTGACG AAGTTGAGCACTGCCAGTCAATTCTTTTCTCCATGTTGCCGAGAGCATCAACATCTAAAGAAATCGATGC TGGACTTCTTTCTATTATTTCTTTCCCGGCCTTTGCAGTAGAAGATGTGCACCTTGTGAATGTGACCAAA AATGAAATTATTTCCAAGCTTCAGGGGCGTTACGGATGCTGTCGCTTCCTTCGGGATGGTTATAAAACCC CAAGAGAGGACCCACACCGATTGCATTATGACCCTGCTGAACTTAAGCTTTTTGAAAACATTGAATGTGA ATGGCCTGTGTTCTGGACTTATTTAATCATAGATGGAGTCTTCAATGGTGATGCTGTTCAGGTCCAGGAA TACCGAGAAGCCCTGGAAGGAATATTAATCAGAGGCAAAGATGGGATCCACTTGGTGCCAGAGCTCTATG CCATCCCACCAGACAAGGTGGATGAAGAGTATAAGAATCCACACACAGTAGACCGAATTCCACTGGGGAA GCTGCCCCATCTTTGGGGACAGTCCTTGTATATCCTCAGCTCACTGCTAGCCGAGGGTTTCCTTGCCACT GGTGAAATTGATCCGTTAAATAGAAGATTTTCTACTTCAGTCAAACCTGATGTTGTAGTCCAAGTTGCTG TTTTGGCAGAAAACAGTCACATTAAGGGGCTCTTGAAGCAACATGGAATAAATGTCCAGAGCATTGCTGA TGTGCATCCAATTCGAGTCCAGCCAGGCCGGATTCTTAGTCACATATATGCCAAATTGGGACAAAATAAG AATATGAAATTGAGTGGTCGACCATATCGGCACATCGGGGTCCTTGGCACCTCTAAACTCTATGTGATTA GGAATCACATCTTCACTTTTACACCCCAGTTCACTGACCAGCATCACTTCTACCTGGCCCTGGACAACGA GATGATTGTGGAGATGCTGAGGATCGAACTGGCCTATCTGTGCACCTGCTGGCGGATGACTGGCCGCCCC ACTCTCACCTTCCCTGTCACACACACCATGCTCACAAATGATGGCTCAGACATTCATCCTGCAGTTCTTT CTACAATTAGAAAACTGGAAGATGGCTATTTTGGAGGTGCTAGAGTAAAACTAGGAATCCTGGCAGAGTT CCTTACTACCTCATTCTACACATACCTGACCTTCCTGGATCCAGACTGTGATGAGAAGTTGTTTGGTGAC ATCAGTGATAGGAGCTTTAGTCCTGACAGTGAGTCAGACCTGGGAGGATACCTTGAAGACAGCAGTCATC AAGAAAGCCAAGATGAACTTGACCAGTATATCAGTCACCTTCTTCAAAGCACATCCTTGAAGTGCTACCT GCCCCCTCTTTGTAAGAAGTCAGAAGACAGCCATGTTTTCAGTGCTGTCCACTCCACTCGGGACATACTT TCTGTGATGGCCAAAGCAAAGGGTTTGGAAACTCCATTTTTTCCCATGATTTTGCCAACTAGAGTTCTAA GTGGACACCGTAAGTCACTGAATCTTGTTGACTCCCCTCAGCCATTCCTAAAGAAGACTCCTGAATATGA CTACCAGTGGCCCAGAGATGACCAGGATGAAGTGGACTGTGAGAAGCTAGTTGGGCAACTGAAAGACTGC TCAAACCTACAGGACCAAGCAGACATTCTGTACATTCTTTATGTAATAAAGGGTCCCCGCTGGGATACCA ATTTGTTTGGACAGCATGGAGTCACTGTTCACAGTCTTCTCGGCGAGCTTTATGGAAAAGCTGGCCTGAA CCAAGAATGGAGTTTGATTCGCTACATTTCAGGCCTGCTCAGGAAGAAAGTGGAGGTCCTGGCTGAGGCC TGTGCAGATCTGCTGTCCCACCAGAAGCAGCTTACAGTGGGCCTGCCCCCTGAGCCCCGGGAGAAGACCA TATCTACGCCTCTTCCCCCAGAGGAGCTCACAGAACTCATATACGAAGCCAGTGGACAGGACATCAGCAT TGCTGTCCTCACACAGGAGATCGTGGTTTACCTGGCCATGTATGTCCGGGCCCAGCCTAGCCTCTTTGCA GAGATGCTCAGACTCCGGATTGGATTGATCATCCAGGTGATGGCCACAGAGCTGGCTCGGAGCCTGAACT GCTCAGGGGAAGAAGCTTCAGAGAGCTTGATGAACCTCAGCCCTTTCGACATGAAGAGCCTTCTGCATCA TATACTGAGTGGGAAGGAGTTCGGCGTAGAGAGAAGTGTGCGCCCAATACACTCCTCCATGTCCAGCCCT GCCATCTCCATCCATGAGGTGGGTCATACTGGAGTCACCAAAACTGAGAGGAGTGGTATCAGCAGACTGA GGAGTGAGATGAAACAGATGAATAGGCGAGCTAGTGCTGATGAGCAGTTCTTTCCACTGGGCCAAGCTGT GCCCAAACGTTTACATTCCATCAAGTCTGCAAGGTCCAGCACACCGTCCTCCCCGACAGGCACATCATCT ACAGACTCTGGAGGACACCACCTGGGCTGGGGAGAACAGCAGGGCCAGTGGCTGCGCAGGAGAAGATTGG ATGGGGCTATCAACAGGGTCCCTGTGGGATTCTACCAGAGAGTGTGGAAGATCCTTCAGAAGTGCCATGG TCTATCCATCGACGGTTATGTCCTCCCATCTTCAACAACCCAAGAGATGACCCCTTGTGAGATCAAGTTC GCTGTCCATGTGGAATCAGTGCTCAACCGTGTGTCCCAGCCCGAGTATCGGCAGCTACTGGTAGAAGCCA TCATGGTACTGACACTGCTCTCAGATACGGAAATGGACAGTATCGGGGGCATCATCCATGTGGACCAGAT AGTGCAGCTAGCCAATCAGCTGTTCCTGCAGGACCAGGTGTCATTTGGAACCACAGATATCCTGGAAAAA GACCAAGCCACAGGAATTTGCCACCTCTTTTATGACAGTGCTCCCAGTGGGGCTTATGGCACAATGACCT ACCTAACGAAAGCAGTAGCTTCTCATTTGCAGGACCTGTTGCCCAGTTCAGGCTGCCAGATGCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001190994
Insert Size	3708 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001190994.1, NP_001177923.1
RefSeq Size	3738 bp
RefSeq ORF	3708 bp
Locus ID	678739

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