Ppfia3 (NM_001270985) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217537 representing NM_001270985 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGCGAGGTGATGCCTACCATCAGCGAGGATGGCCGGCGGGGCTCGGCGCTGGGCCCGGACGAGG CCGGCGGCGAGCTGGAGCGCCTCATGGTCACGATGCTCACGGAGCGCGAGCGCCTGCTGGAGACGCTGCG AGAGGCGCAGGACGGGTTGGCGACAGCGCAGCTACGACTGCGCGAGCTCGGCCACGAGAAGGACTCGCTG CAGCGGCAGCTCAGCATTGCTCTGCCTCAGGAGTTTGCGGCTCTGACGAAGGAGCTTAACTTATGTCGGG AACAGCTTCTGGAGAGGGAAGAAGAAATCGCAGAGCTGAAGGCGGAGCGAAACAACACCCGGCTACTTCT GGAACACCTCGAGTGCCTAGTGTCCAGGCACGAGCGTTCCCTACGAATGACTGTGGTGAAACGCCAAGCC CAATCTCCTGGAGGAGTCTCCTCTGAGGTGGAAGTGCTCAAGGCTCTAAAATCCCTCTTTGAGCACCACA AGGCCCTGGATGAGAAGGTCCGGGAAAGATTGCGGATGGCACTGGAGAGGGTAGCAGTGCTCGAGGAGGA GCTGGAACTGAGCAATCAGGAGGCCCTGAACCTTCGAGACCAGCTATCTAGAAGGCGGTCAGGACTGGAG GAGCCGGGCAAGGATGGGGATGGACAGACCCTTGCCAATGGCCTGGGTCCCGTTGGAGAATCCAGCCGGC GCACGGCAGAGCTGGAGGAGGCACTGGAGCGGCAGCGTGCAGAGGTGTGCCAGCTGAGGGAGCGCCTGGC GGTGCTGTGCCGTCAGATGAGCCAGCTGGAGGAGGAGCTGGGCACTGCTCACCGCGAGCTGGGTAAGGCG GAGGAAGCTAACTCCAAGCTGCAGCGCGACCTCAAGGAGGCCCTGGCTCAGCGGGAAGATATGGAAGAAC GCATCACCACACTGGAAAAGCGCTATCTGAGTGCCCAGCGTGAGGCCACATCTCTACATGATGCCAACGA TAAGCTGGAGAATGAGCTGGCCAGCAAAGAGTCCCTGTACAGACAGAGTGAAGAGAAGAGCCGTCAGCTG GCCGAGTGGCTGGATGACGCCAAGCAGAAGCTGCAGCAGACCCTGCAGAAGGCAGAGACCTTGCCTGAGA TTGAGGCTCAGCTGGCACAGAGAGTGGCTGCTCTGAACAAGGCTGAGGAACGCCATGGGAACTTTGAGGA GAGGCTTCGGCAGCTGGAGGCACAGCTGGAGGAGAAGAACCAGGAGCTGCAGAGGGCCCGGCAGAGGGAG AAGATGAACGATGACCACAATAAACGACTATCAGAAACGGTGGACAAGCTGCTAAGTGAATCCAATGAAC GGTTACAGCTTCACCTCAAGGAACGTATGGGGGCACTGGAGGAGAAGAACTCACTGAGTGAGGAGATTGC TAACATGAAGAAGCTTCAAGATGAGCTGCTCCTGAATAAGGAGCAGCTCTTGGCTGAGATGGAACGGATG CAGATGGAGATCGACCAGCTACGGGGGAGGCCGCCCTCCTATTCCAGGTCCCTCCCTGGCAGTGCCCTGG AGCTCCGGTACTCCCAGGCACCAACCTTACCTTCTGGTGCCCCTCTGGATCCTTATGGGGCAGGCAGTGG CCGGGCAGGCAAGAGGGGCCGCTGGTCGGGGGCCAAGGACGAGTCTTCCAAGGACTGGGACCGGTCTACC CCTGCTGGATCCATTCCACCCCCTTTCCCTGGAGAGCTAGATGGCTCAGATGAGGAGGAGACCGAGGGGA TGTTCGGGGCTGAACTGCTGTCCCCCAGTGGGCAAGCTGATGTGCAGACACTGGCCATCATGCTCCAGGA GCAATTGGAAGCCATCAACAAGGAAATCAAGCTAATTCAAGAAGAGAAAGAAACGACAGAGCAGCGTGCA GAGGAGCTGGAGAGCCGGGTGTCCAGTTCTGGCCTCGACTCTCTGGGCCGGTACCGCAGCAGCTGTTCAC TACCGCCCTCCCTCACCACCTCCACCCTCGCCAGCCCCTCACCTCCCAGCTCCGGACACTCCACCCCTCG TCTGGCACCTCCCAGTCCTGCCCGGGAGACCGACAAGACCAATCATGTCCCCAAGGACGAAGCCGGTGTT CCACGAGGGGAGGGTCCAGCCATTCCAGGAGACACCCCACCACCCACTCCCCGCTCTGCCCGTCTTGAGA GGATGACCCAGGCTTTGGCACTGCAAGCGGGGTCACTGGAAGATGGGGCACCTCCCCGGGGAAGTGAGAG CACCCCAGATTCCCTCCACAAAGCCCCCAAGAGGAAGAGCATCAAGTCATCCATCGGTCGGCTCTTCGGC AAGAAAGAGAAGGGCCGGATGGGACCCCCAGGCCGGGAAAGTGTTTCTCTGGCTGGGACACCGTCTGATG AAACACTGGCCACTGACCCTCTGGGACTAGCCAAGCTGACTGGTCCAGGAGATAAGGACAGGAGGAACAA GAGAAAGCATGAACTTCTAGAAGAGGCCTGTCGACAAGGCCTGCCTTTTGCTGCCTGGGACGGCCCCACC GTGGTCTCCTGGCTGGAGCTCTGGGTGGGCATGCCTGCATGGTACGTGGCCGCCTGCCGGGCCAATGTCA AGAGCGGCGCCATCATGGCCAACCTGTCAGACACGGAGATCCAGCGGGAAATCGGCATCAGCAATCCACT GCACAGGCTCAAACTGCGCCTTGCCATTCAGGAGATGGTATCACTCACATCACCCTCTGCTCCTGCCTCC TCACGCACGCCCACAGGAAACGTGTGGATGACACACGAGGAGATGGAGTCTCTGACTGCAGCTACAAAAC CCGAGACCAAGGAGATCAGCTGGGAGCAGATCCTGGCATATGGCGACATGAACCACGAGTGGGTGGGCAA CGACTGGCTGCCCAGCCTTGGGCTGCCCCAGTACCGAAGCTACTTCATGGAGTCGCTAGTTGATGCTCGG ATGTTGGACCATCTGAACAAGAAAGAACTGCGAGGCCAACTCAAGATGGTGGACAGCTTCCACAGGGTTA GTCTGCACTATGGTATCATGTGCTTGAAACGGCTCAACTATGACCGGAAGGATCTGGAGCGAAGGAGGGA AGAAAGCCAGACCCAAATCAGAGATGTGATGGTGTGGTCCAACGAACGGGTCATGGGTTGGGTGTCTGGT CTTGGCCTGAAGGAGTTTGCTACGAATCTCACCGAGAGTGGGGTGCACGGAGCCCTGTTGGCTCTGGATG AAACCTTCGACTACTCCGACCTGGCCTTGCTTCTACAAATACCTACACAGAATGCACAAGCTCGGCAACT GCTGGAGAAAGAATTCAGTAACCTCATCTCCCTAGGAACAGACAGACGGCTGGATGAGGACAGTGCCAAG TCCTTCAGCCGCTCCCCATCCTGGAGGAAGATGTTCCGTGAGAAGGACCTCCGGGGTGTAACCCCCGATT CAGCCGAGATGTTACCCCCCAACTTTCGTTCAGCTGCAGCAGGAGCCCTAGGCTCACCGGGACTCCCACT CCGCAAACTGCAGCCTGAAGGCCAGACTTCTGGGAGTTCACGAGCAGACGGTGTTTCGGTCCGGACTTAC TCCTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270985
Insert Size	3579 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270985.2, NP_001257914.1
RefSeq Size	4643 bp
RefSeq ORF	3579 bp
Locus ID	140591
Gene Summary	may play a role in integrating active zone proteins and synaptic vesicles [RGD, Feb 2006]

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