Ciita (NM_001270803) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	C2ta; Mhc2ta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217454 representing NM_001270803 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTGCCAGGTTCCCGGCCCTTCTGGGTCTTACCTGCAAGGCCATACTCTGTGTGCCACCATGGAGC TGGGGTCTCCAGAGGGCAGCTACCTGGAGCTCCTTAACAGTGATGCTGACCCCCTACACCTCTACCACCT CTATGACCAGATGGACCTTGCTGGGGAGGAAGAGATCGAACTCAACTCAGAGACAGACATGGACACCATC AACTGTGACCAGTTCAGCAAGCTGTTGCAGGATATGGAAATGGATGAAGAGACCCGGGAGGCCTATGCCA ATATCGCGGAACTGGATCAGTACGTGTTCCAGGATACCCAGCTGGAGGGCCTGAGCAAGGACCTCTTCAT ACAGCACATTGGACCAGAGGAAGGCTTTGGTGAGAACGTAGAGGGCCCTGCAGAATCAGGAGAGAAGCCT CAGAAGAGACGCTTCCCAGAAGAGCATGCTATGGACTCAAAGCACAGGAAACTAGTGCCTCCCTCTAGGA CCTCACTGAACTATTTGGATCTCCCCGCTGGGCATATCCAGATCTTCACCACTCTGCCCCAGGGACTCTG GCAAATCTCAGGGGTTGGCACAGGGCTCTCCAGTGTCCTAGTCTACCACGGTGAGATGCCCCAGGTCAAC CAATTACTCTCTTCAAGCAGCCCCACTGTCCCCAGTCTCCCCGAGTCCCCAGACCGGCCTGGCTCCACCA GCCCCTTCGCGCCATCCACAGCTGACCTGCCCGGCATGCCTGAACCTGCTCTGACCTCCCGTGTAAATGA GACAGAGGACACAACACCCCCCTCCCCATGCCAAGAGGATCCCGAGCCTTCCATCAAGCTTCCAAAATGG CCAGAGTCCGTGGAGCGGTTCCAGCACTCCCTACAGGACAAGTACCAAGCACTGCCCGGGAGCCCAAGTG GTCCTCTGGTAGCCATGGAGCTGGTGCGGGCCAGGTTGGAAAGAGGTAGCAACAAGAGTCAGGAAAGGGA GCTGGCCACTCCTGACTGGACAGAGCGCCAGCTAGCCCATGGCGGTCTGGCCGAGGTACTTCGGGTTGTC GGCGACTCCAGGAGATCACGAGAGACACAGGTGGTCGCTGTGCTGGGCAAGGCTGGTCAGGGCAAGAGCC ACTGGGCCAGGACAGTGAGCCACATTTGGGCACAAGGCCAGTTACCACAATATGACTTTGTCTTCTACGT CCCCTGTCAATGCTTGGATCGCCCTGGGGACACCTACCACCTGCGGGATCTGCTCTGTCCCCCGAACCTG CAGCCACTGGCCATGGATGACGAGGTCATTGATCATATTGTGAGGCAGCCGGACCGTGTTCTGCTCATCC TAGATGCTTTTGAGGAGCTAGAGGCCCAAGACAGCCTCCTGCATGGACCCTGTGGACCTCTGTCCCCAGA GCCCTGCTCCCTCCGGGGACTGCTGGCTGGGCTCTTCCAGCGTAAGCTGCTGCGAGGCTGCACCCTGCTC CTCACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCAAGGCAGATGCCATCTTCGAGGTGCCCA GCTTCTCTACGAAACAGGCCCAGATCTATATGAGGCACTACTTTGAGAACTCAGGGACAGCAGGGAGTCA CGACAAGGCCCTGGGCCTCCTGGAGGGCCAGCCTTTTCTCCTCACCCACAGTCACAGCCCTGTTCTGTGC AGTGCCGTGTGTCAGCTCTCCAAGGCCCTGCTGGATCAGGGCGCAGAGGCCCAGCTACCTTGCACACTCA CAGGACTCTATGTTGGCCTGCTAGGCCCTGCAGCTCAGAACAGTCCTCCTGGAGCCTTAGTTGAGTTGGC CAAGCTAGCCTGGGAGCTGGGACGGAGGCACCAAAGCACCTTGCAGGAAACCCAGTTTCCATCCGTGGAA GTGAAGACCTGGGCTGTGGCCCAAGGCTTGATGCAGCAGACCACAGAGGCCCAACTGGCTTTCTCCAGTT TTCTGCTACAGTGCTTCCTGGGTGCTGTGTGGCTGGCACAGTGCAATGAGATGAAAGACAAGGAGCTGCC ACAGTACCTGGCCTTGACCCCGAGGAAGAAGCGACCCTATGACAACTGGCTGGAGGGCGTACCACGCTTT CTGGCTGGCTTAGTTTTCCAGCCTCGAGCCCACTGCCTGGGAGCTCTGGTGGAGCCTGCAATGGCTGCAG TGGCAGATAGGAAACGGAAGGTTCTTACCAGGTACCTGAAGCGCTTGAAGCTGGGGACACTTCGGGCCGG GAGGTTGCTGGAGCTGCTACACTGTGCCCACGAAACACAGGAACCTGGTATTTGGAAGCATGTTTCACAC CAGCTCCCAGGGCACCTCTCCTTCCTGGGCACACGGCTCACAGCCCCAGATGTGTATGTTCTGGGCAGGG CCTTGGAGACAGCCAGCCAGGACTTCTCCTTGGACCTTCGTCAGACTGGCATCGAGCCCTCCAGACTGGG AGACCTCGTGGGACTCAGCTGTGTCACCAGTTTCAGGGCCTCCTTGAGTGACACAATGGCATTATGGGAG TCCCTACAGCATCAGGGAGAAACCCAGCTACTCCAGGCGGCAGAGGAGAAGTTCACCATTGAGCCATTTA AGGCCAAATCCCCAAAGGATGTGGAAGACCTGGACAGCCTTGTGCAGACCCAGAGGCTGAGAAACCCCTC AGCGGATGCAGCCAAGGATCTTCCTGCCATCCGGGACCTTAAGAAGCTAGAGTTTGCATTGGGCCCCGTC TTGGGCCCGCAGGCTTTCCCCACACTGGCCAAGATCCTTCCAGCATTCTCTTCCCTGCAGCACCTGGACC TGGACTCACTTAGCGAGAACAAGATCGGGGACAAGGGTGTGTCGAAGCTCTCAGCCACCTTCCCTCAGCT GAAGGCCCTGGAGACGCTCAACCTGTCCCAGAATAGCATCACCGATGTGGGAGCTTGCAAGCTTGCAGAA GCCCTGCCGGCCCTGGCCAAGTCCCTCCTAAGGCTGAGCTTGTACAACAACTGCATCTGTGACGAAGGGG CCAAGAGCCTGGCACGAGTGCTTCCAGACATGGTGTCCCTCCGGGTGATGGATGTCCAGTTCAACAAGTT CACGGCTGTCGGGGCCCAGCAGCTGACCTCCAGCCTTCAGAAGTGCCCTCAGGTGGAGACACTGGCAATG TGGACACCCACTATCCCCTTTGGGGTCCAGGAACACCTGCAGCAGCTGGATGCCCGGATTAGCCTGAGAT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270803
Insert Size	3222 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270803.1, NP_001257732.1
RefSeq Size	5076 bp
RefSeq ORF	3222 bp
Locus ID	85483
Gene Summary	may play a role in transcriptional activation of MHC class II genes [RGD, Feb 2006] Transcript Variant: This variant (2) has an alternate first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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