Myo1a (NM_019324) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	BBMI; MIHC; Myh1; Myhl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217436 representing NM_019324 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCTCCTGGAAGGTTCCGTGGGGGTGGAGGATCTCATCCTCCTGGAGCCTCTGGATGAGGAGTCTC TGATCAAGAACCTCCAACTTCGTTATGAGAAAAAAGAGATTTATACGTACATTGGGAATGTGGTGATCTC CATGAACCCCTACCAGCAGCTTCCTATCTATGGCCCAGAGTTTATTGCCAAATACCGGGACTATACCTTC TATGAGCTGAAGCCCCACATCTATGCTTTGGCAAACGTGGCCTACCAGTCGCTGAAGGATCGGGACCGGG ACCAGTGTATCCTGATCACTGGCGAGAGTGGAGCTGGCAAGACTGAGGCTAGTAAGCTAGTGATGTCCTA TGTGGCTGCTGTCTGTGGGAAAGGGGAGCAGGTGAACTCGGTGAAGGAGCAGCTGCTTCAGTCAAACCCA GTGCTGGAAGCATTTGGCAATGCCAAGACCATCCGCAATGACAACTCCTCTCGATTTGGAAAATACATGG ACATCGAGTTTGACTTCAAAGGGTCCCCCCTTGGAGGTGTCATTACCAACTATCTGCTTGAGAAGTCCAG AGTGGTGAAGCAACTCAAAGGGGAAAGGAACTTCCATATCTTCTATCAGCTGCTGGCTGGAGCAGACACA CAGCTGCTGAAGGCCCTAAAGCTTGAGGAAGATGCACGTGGCTATGCCTACCTTAATGGGGAGGTGTCCA GGGTGAACGGTATGGATGATGCCTCCAACTTCAGGGCTGTGCAGAACGCAATGGCAGTGATTGGGTTCTC AGAGGAGGAGATTAGACAAGTGCTGGAGGTGACAGCCCTGGTACTGAAGCTGGGCAATGTGAAGCTGGCA GATGAATTCCAGGCCAACGGGCTTCCAGCCAGTGGTGTCTGTGATGGGAAAGGTATTCAGGAGATTGGGG AGATGATGGGTTTGAACTCTGCAGAACTAGAGAAAGCTTTGTGCTCAAGAACCATGGAGACAGGCAAAGA GAAGGTGGTCACGGTGCTGAACGTCATCCAGGCTCAGTATGCTCGGGATGCCCTGGCCAAGAATATTTAC AGCCGCCTCTTCGACTGGATAGTGAATCGCATCAACGAGAGCATCAAGGTGGGCACTGGGGAGAAGAGGA AGGTTATGGGGGTCCTGGATATCTACGGCTTTGAGATACTGGAGGATAACAGCTTTGAGCAGTTCGTGAT CAACTACTGCAATGAAAAACTACAGCAGGTGTTTATAGAACTGACCTTGAAGGAAGAACAGGAGGAATAT AAGAGAGAAGGCATACCATGGACAAAGGTGGAGTACTTTGACAACGGCATCATCTGTGACCTCATTGAAC ATAGTCAGCGAGGCATCCTGGCCATGCTGGATGAGGAGTGCCTGAGACCTGGGGTGGTCAGTGACACCAC TTTTCTAGCAAAGTTGAACCAGCTTTTCTCCAAGCACAGTCACTACGAGAGTAAAGTCACCCAGAATGCT CAGCGCCAATACGACCGCACCATGGGCCTCAGCTGCTTCCGCATCTGCCACTATGCGGGCAAGGTGACAT ACAACGTGACCAGTTTCATCGACAAGAATAACGACCTACTCTTCCGAGACCTATCCCAGACAATGTGGAA GGCCCAGCATCCCCTCCTCCACTCCCTGTTCCCAGAGGGGAATCCCAAGGAAGCATCTCTCAAACGGCCC CCGACTGCTGGGACCCAGTTTAAGAATTCGGTGGCCATACTCATGAAGAACCTGTATTCTAAGAACCCGA ACTACATCAGGTGCATAAAGCCCAATGACCAGCAGCAGCAAGGCAGGTTCACCTCAGAACTGGTTATGGT CCAGGCTCGATACCTGGGGCTGCTGGAGAATGTTCGGGTGCGCAGGGCTGGCTACGCCTTCCGCCAGGCC TATAAGCCTTTCCTGGAGAGGTACCGATTGCTGAGCCGGAGCACGTGGCCGCGCTGGAATGGGGAAGACC GGGAAGGTGTGGAGAAGGTCCTGGGGTCTCTGATTTTGTCATCAGAGGAGCTGGCCTATGGGAGGACAAA GATCTTCATCAGAAGCCCCAAGACTCTTTTCTACCTGGAAGAACAAAGGCGCCTGAGGCTTCAGCAGCTG GCCACCCTCATCCAGAAGGTTTACCGGGGCTGGCGTTGTCGGACCCACTACCAGCAGATGCGGAAGAGTC AGATCCTCATCTCGGCTTGGTTTCGGGGCAACAAGCAAAAGAAGCACTATGGGAAGATCCGGTCATCAGT ACTGCTGATCCAGGCTTTCGTGAGGGGCTGGAAGGCCCGCAAGAACTATCGAAAATACTTCCGGTCAGGC GCCGCCATCACTTTGGCAAATTTTATCTACCAGAGCATAACCCAGAAATTCCTCTTGAATCTGAAGAAGA ATTTGCCATCCACCAAAGTGTTGGACAACACGTGGCCAGCTGCCCCTTACAGGTGCTTCAACACAGCCAA TCAGGAACTGCAGCACCTCTTCTACCAGTGGAAGTGCAAGAAGTACCGAGATCAGCTGTCCCCGAAGCAG GTGCAGACACTGAGGGAAAAGCTCTGTGCCAGCGAACTGTTTAAGGGCAAGAAGGCTTCCTACCTCCAGA GCGTGCCTGTCCCTTTCCGTGGTGATTACGTCGGCCTGCAGGGGAACCCCAAGCTGCAGAGACTGAAGGG CAGGGAGGAAGGGCCTGTCCTGGTGGCAGACACCGTGAAGAAGGTCAACCGTGGCAATGGCAAGACTTCT TCACGGATTCTCCTCCTGACCAAGGGGCATGTGATTCTCACAGATGCCAAGAAGTCCCAGGCCCAAATTG TCATTAAGCTGGAGGATGTGGCTGGGGTGTCAGTCACCAGCCTCGAGGACGGGCTCTTTAGCTTGCATCT GAGTGAGATGTCGTCGGCAGCCTCCAAGGGGGACATCCTGCTAATCAGCGACCACCTGGTTGAACTGCTG ACAAAAATGTACCAAGCAGTGCTGGATGCCACACAGAGGCAGCTTCCTGTCACTGTGACTGAGAAGTTCT CAGTGAGGTTCAAGGAGGGCAGTGTGGCTGTCAAGGTTACCCAGGGCCCCCAGGGGAGTGGGAACAGAAA GCTGACTTGCAAGAAGAAGGGGAGTAGTTCTATGGAGGTGACTGTGCGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019324
Insert Size	3132 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019324.1, NP_062197.1
RefSeq Size	3332 bp
RefSeq ORF	3132 bp
Locus ID	299509
Gene Summary	myosin isoform that connects actin filaments to the membrane in intestinal microvilli [RGD, Feb 2006]

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