Nlrp3 (NM_001191642) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cias1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217429 representing NM_001191642 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATGATGAGTGTTCGCTGCAAGCTGGCCCAGTATCTAGAGGACCTGGAAGATGTGGACCTCAAGA AATTTAAAATGCATTTGGAAGATTACCCACCCGAGAAAGGCTGTGTTCCGATCCCCAGGGGCCAGATGGA GAAGGCAGATCACTTGGATCTAGCCACACTCATGATTGACTTCAATGGGGAGGAGAAGGCGTGGGGCATG GCTGTGTGGATCTTTGCAGCGATCAACAGGCGAGACCTCTGGGAAAAAGCTAAGAAGGACCAGCCAGAGT GGAATGATGCATGCACGTCTAATCTCTCCATGGTGTGCCAGGAAGACAGCCTTGAAGAGGAGTGGATAGG TTTGCTGGGATATCTCTCCCGCATCTCGATTTGTAAAAAAAAGAAAGATTACTGTAAGATATACAGAAGG CATGTGAGAAGCAGGTTCTACTCCATCAAAGACAGGAATGCACGTCTAGGTGAGAGCGTGGACCTCAACA GACGCTACACCCAGCTCCAACTGGTCAAGGAGCATCCAAGCAAGCAGGAAAGGGAGCATGAACTCTTGAC CATTGGCAGGACTAAGATGTGGGACAGGCCCATGAGCTCCCTTAAGCTGGAGCTGCTCTTTGAGCCTGAG GATGAACACTTGGAGCCCGTGCACACAGTGGTGTTCCAGGGAGCAGCAGGCATCGGAAAAACAATCCTAG CCAGGAAGATTATGTTGGACTGGGCCTTGGGGAAGCTCTTCAAAGACAAATTTGATTATTTGTTCTTTAT CCACTGTCGAGAGGTGAGCCTCAGGGCACCAAAGAGCCTAGCAGATCTCATTATCAGCTGCTGGCCTGAC CCAAACCCACCAGTCTGCAAGATCCTGTGCAAGCCTTCCAGGATCCTCTTCCTCATGGACGGCTTTGATG AGCTGCAGGGGGCCTTTGATGAGCACATCGAGGAGGTCTGCACAGACTGGCAGAAGGCTGTGAGGGGAGA CATTCTGCTAAGCAGCCTCATCCGAAAGAAGTTGCTGCCTAAGGCCTCTCTGCTTATTACGACGAGGCCA GTAGCCTTGGAGAAGCTGCAGCATCTCCTGGACCACCCCCGCCACGTGGAGATCCTAGGTTTCTCTGAGG CCAAACGGAAGGAGTATTTCTTTAAGTATTTCTCAAACGAGCTGCAGGCCAGGGAGGCCTTCAGGCTGAT CCAAGAGAATGAGATCCTGTTTACTATGTGCTTCATCCCCCTGGTCTGCTGGATTGTGTGCACAGGGCTA AAGCAGCAGATGGAGACTGGAAAGAGCTTGGCCCAGACCTCCAAGACCACGACTGCCGTCTATGTCTTTT TCCTTTCCAGCCTGCTGCAGTCCCGGGGGGGCATTGAGGAGCACCTCTTCTCTGCCTACCTGCCAGGGCT CTGTTCATTGGCTGCGGATGGAATTTGGAACCAGAAGATCCTATTTGAAGAGTGTGATCTGCGGAAACAC GGCCTGCAGAAGACCGACGTCTCTGCTTTCCTGAGAATGAACGTGTTCCAGAAGGAGGTGGACTGCGAGA GATTTTACAGCTTCAGCCACATGACTTTCCAGGAGTTCTTTGCGGCTATGTACTATCTGCTAGAAGAGGA GGAAGAGGGGGTGACCGTGAGGAAGGGACCAGAAGGCTGTTCAGACCTTCTGAACCGAGACGTGAAGGTC CTATTAGAAAATTACGGCAAGTTCGAAAAAGGCTATCTGATTTTTGTTGTACGCTTCCTCTTTGGCCTTG TAAACCAGGAGAGAACTTCTTATTTGGAGAAGAAGCTAAGTTGCAAGATCTCTCAGCAAGTCAGACTGGA GCTGCTGAAGTGGATCGAAGTGAAAGCCAAGGCCAAGAAGCTGCAGAGGCAGCCCAGCCAGCTGGAACTG TTCTACTGCTTGTACGAGATGCAGGAGGAAGACTTTGTGCAGAGCGCCATGGGCCACTTCCCCAAAATTG AGATCAACCTCTCTACCAGAATGGACCACGTGGTTTCTTCCTTTTGCATTAAGAATTGCCATAGGGTAAA AACACTTTCCCTGGGATTTCTCCACAACTCACCCAAGGAGGAAGAAGAAGAGAAAAGAGGAAGTCAACCC TTGGACCAGGTCCAGTGTGTTTTCCCAGACCCTCATGTTGCCTGTTCTTCCAGACTGGTGAACTGCTGCC TCACCTCTAGCTTCTGCCGAGGTCTCTTCTCAAGTCTCAGCACCAACCAGAGCCTCACTGAACTGGACCT CAGTGACAATACTCTAGGGGATCCAGGCATGAGGGTGCTGTGTGAGGCACTCCAGCACCCAGGCTGTAAC ATTCAGAGACTGTGGTTGGGGCGCTGCGGACTGACCCATCAATGCTGTTTCAACATCTCCTCTGTCCTGA GCAGCAGCCAGAAGCTGGTGGAGCTGGACCTCAGTGACAATGCCCTGGGGGACTTTGGAGTCAGACTGCT GTGCGTGGGACTGAAGCATCTGCTCTGCAACCTGCAGAAACTCTGGTTGGTCAGCTGCTGTCTCACATCT GCGTGTTGTCAGGATCTCGCATTGGTTCTGAGCTCCAACCATTCTCTGACCAGACTGTACATTGGAGAAA ATGCCTTGGGAGACTCAGGAGTTCAAGTTTTGTGTGAAAAAATGAAGGACCCACAGTGTAACTTGCAGAA GCTGGGGTTGGTGAATTCCGGCCTTACTTCACTGTGCTGCTCAGCTCTGACCTCTGTGCTCAAAACCAAC CAGAACCTCACACACCTGTACCTACGAAGCAATGCCCTTGGAGACATGGGACTCAAGCTCCTCTGTGAGG GACTTCTGCACCCCGACTGTAAGCTACAGATGCTGGAGTTAGACAACTGCAGCCTCACCTCACACTCCTG CTGGGATCTCTCCACAATTCTGACCCATAACCAGAGCCTCCGGAAGCTGAACTTGAGCAACAACGACCTG GGCGATCTGTGTGTGGTGACGCTCTGTGAGGTTCTGAAACAGCAGGGCTGCCTCCTGCAGAGCCTACAGT TGGGTGAAATGTATTTAAATTGTGAAACAAAACGTACCTTAGAAGCTCTCCAGGAGGAAAAGCCTGAGCT GACTGTTGTTTTTGAGATTTCTTGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191642
Insert Size	3108 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191642.1, NP_001178571.1
RefSeq Size	3857 bp
RefSeq ORF	3108 bp
Locus ID	287362
UniProt ID	D4A523
Gene Summary	As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion (By similarity). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33 (By similarity). Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (By similarity).[UniProtKB/Swiss-Prot Function]

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