Hyou1 (NM_001034028) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cab140; Orp150
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217402 representing NM_001034028 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCTACAGTAAGGAGGCAGAGGCCAAGGAGGCTACTCTGTTGGGCCTTGGTGGCTGTCCTCTTGG CAGACCTGTTGGCACTGAGTGACACACTGGCTGTGATGTCTGTGGACCTGGGCAGTGAATCCATGAAGGT GGCCATTGTCAAGCCTGGAGTGCCCATGGAGATTGTATTGAACAAGGAATCTCGGAGGAAAACTCCGGTG ACTGTGACCTTGAAGGAAAACGAAAGGTTTCTAGGTGACAGTGCAGCTGGCATGGCCATCAAGAACCCAA AGGCTACGCTCCGTTATTTCCAGCACCTCCTTGGAAAGCAGGCAGATAACCCTCATGTGGCTCTTTACCG GTCCCGTTTCCCAGAACATGAGCTCAATGTTGACCCACAGAGGCAGACTGTGCGCTTCCAGATCAGTCCG CAGCTGCAGTTCTCTCCCGAGGAGGTGCTGGGCATGGTTCTCAACTACTCCCGTTCCCTGGCTGAAGATT TTGCAGAACAACCTATTAAGGATGCAGTGATCACCGTGCCAGCCTTTTTCAACCAGGCCGAGCGCCGAGC TGTGCTGCAGGCTGCTCGTATGGCTGGCCTCAAGGTGCTGCAGCTCATCAATGACAACACTGCCACAGCC CTCAGCTATGGTGTCTTCCGCCGGAAAGATATCAATTCCACTGCACAGAATATCATGTTCTATGACATGG GCTCGGGCAGCACTGTGTGTACCATCGTGACCTACCAAACGGTGAAGACTAAGGAGGCTGGGACGCAGCC ACAGCTACAGATCCGGGGCGTGGGATTTGACCGCACCCTGGGTGGCCTGGAGATGGAGCTTCGGCTGCGA GAGCACCTGGCTAAGCTCTTCAATGAGCAGCGCAAGGGCCAGAAAGCCAAGGATGTTCGGGAAAACCCCC GAGCCATGGCCAAACTGCTTCGGGAAGCCAATCGGCTTAAAACCGTCCTGAGTGCCAATGCTGATCACAT GGCACAGATTGAAGGCTTGATGGACGATGTGGACTTCAAGGCAAAAGTAACTCGAGTGGAGTTTGAGGAG CTGTGTGCAGATTTGTTTGATCGAGTGCCTGGGCCTGTACAGCAGGCCCTGCAGAGTGCTGAGATGAGCC TGGATCAAATTGAGCAGGTGATCCTGGTGGGTGGGGCCACTCGTGTTCCCAAAGTTCAAGAGGTGCTGCT GAAGGCTGTGGGCAAGGAGGAACTAGGAAAGAACATCAATGCCGATGAAGCAGCTGCCATGGGGGCCGTG TACCAGGCAGCGGCACTGAGCAAAGCCTTCAAAGTGAAGCCATTTGTTGTGCGTGATGCTGTTATTTACC CCATCCTGGTGGAGTTCACAAGGGAGGTGGAGGAGGAGCCTGGGCTTCGAAGCCTGAAGCACAATAAACG TGTGCTCTTCTCCCGAATGGGGCCCTACCCTCAGCGCAAAGTCATCACCTTTAACCGATACAGCCATGAT TTCAACTTTCACATCAACTACGGTGACCTGGGCTTCCTGGGGCCTGAGGATCTTCGGGTATTTGGCTCCC AGAATCTGACCACAGTGAAACTAAAAGGTGTGGGAGAGAGCTTCAAGAAATATCCTGACTATGAGTCCAA AGGCATCAAGGCCCACTTTAACCTAGACGAGAGTGGAGTGCTCAGTTTAGACAGGGTGGAGTCCGTATTC GAGACCCTGGTGGAGGACAGCCCAGAGGAAGAGTCTACTCTTACCAAACTTGGCAACACCATTTCCAGCC TGTTTGGCGGTGGTACCTCATCAGATGCCAAAGAGAATGGTACTGATGCTGTACAGGAGGAGGAGGAGAG CCCTGCTGAGGGGAGCAAGGATGAGCCTGCAGAACAGGGGGAACTCAAGGAGGAAGCTGAAGCCCCAATG GAGGATACCTCCCAGCCTCCACCCTCTGAGCCTAAGGGGGATGCAGCCCGTGAGGGAGAAACACCTGATG AAAAAGAAAGTGGGGACAAGTCTGAGGCCCAGAAGCCCAATGAGAAGGGGCAGGCAGGGCCTGAGGGTGT CCCTCCAGCTCCCGAGGAAGAAAAAAAGCAGAAACCTGCCCGGAAGCAGAAAATGGTGGAGGAGATAGGT GTGGAACTGGCTGTCTTGGACCTGCCAGACTTGCCAGAGGATGAGCTGGCCCATTCCGTGCAGAAACTTG AGGACTTGACCCTGCGAGACCTTGAAAAGCAGGAGAGGGAGAAAGCTGCCAACAGCTTAGAAGCTTTTAT CTTTGAGACCCAGGACAAACTGTACCAACCTGAGTACCAGGAAGTGTCCACTGAGGAACAACGGGAGGAG ATCTCTGGAAAACTCAGTGCCACTTCTACCTGGCTGGAGGATGAGGGATTTGGAGCCACCACTGTGATGT TGAAGGACAAGCTGGCTGAGCTGAGAAAGCTGTGCCAAGGGCTGTTTTTTCGGGTGGAAGAGCGCAGGAA ATGGCCAGAGCGGCTTTCAGCTCTGGATAATCTCCTCAATCACTCCAGCATTTTCCTCAAGGGTGCCCGA CTCATCCCAGAGATGGACCAGATCTTCACTGACGTGGAGATGACAACGTTGGAGAAAGTCATCAATGACA CCTGGACCTGGAAGAATGCAACCCTGGCCGAGCAGGCCAAGCTTCCTGCCACAGAGAAACCCGTGCTGCT TTCAAAAGACATCGAGGCCAAAATGATGGCCCTGGACCGGGAGGTGCAGTATCTACTCAATAAGGCCAAG TTTACTAAACCCCGGCCACGGCCCAAGGACAAGAATGGCACCCGGACAGAGCCTCCCCTCAATGCCAGTG CTGGTGACCAAGAGGAAAAGGTCATTCCACCTACAGGCCAGACTGAAGAGGCGAAGCCCATCTTAGAACC TGACAAAGAGACTGGTACAGAGCCAGCAGACTCTGAGCCTCTGGAATTAGGAGGTCCTGGTGCAGAATCT GAACAGGCAGAGCAGACAGCAGGGCAGAAGCGGCCTTTGAAGAATGATGAGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001034028
Insert Size	2997 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001034028.1, NP_001029200.1
RefSeq Size	4346 bp
RefSeq ORF	2997 bp
Locus ID	192235
Gene Summary	a stress protein induced by hypoxia [RGD, Feb 2006]

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