Slc8a1 (NM_001270772) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ncx; Ncx1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217348 representing NM_001270772 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCGACTAAGTCTCCCACCCAATGTTTCAATGGGATTTCGTCTGGTAACTCTGGTGGCTCTCTTGT TTACCCATGTTGACCATATAACTGCAGATACAGAGGCAGAAACAGGAGGAAATGAAACCACTGAATGTAC TGGCTCATATTACTGTAAGAAAGGGGTGATTCTGCCCATTTGGGAACCCCAAGACCCATCTTTTGGGGAT AAAATTGCTAGAGCAACTGTGTATTTTGTGGCCATGGTCTACATGTTCCTCGGAGTTTCTATTATTGCCG ACCGGTTTATGTCCTCTATAGAAGTCATCACCTCTCAAGAAAAGGAGATTACCATAAAGAAACCAAATGG AGAGACCACCAAGACTACAGTGCGTATCTGGAATGAGACTGTGTCCAACCTGACCTTGATGGCCCTGGGA TCTTCCGCTCCTGAGATTCTCCTGTCTGTCATTGAAGTGTGTGGCCATAACTTCACCGCAGGGGACCTTG GTCCCAGCACCATTGTGGGAAGCGCCGCCTTCAACATGTTCATCATCATCGCGCTTTGTGTTTATGTGGT CCCAGATGGAGAGACGAGGAAGATTAAACATCTGCGTGTGTTCTTTGTGACAGCAGCCTGGAGCATCTTT GCCTATACCTGGCTTTACATAATTTTGTCTGTCAGCTCTCCTGGTGTCGTGGAGGTCTGGGAAGGATTAC TCACTTTCTTCTTCTTTCCCATCTGTGTTGTGTTCGCTTGGGTTGCAGACAGGCGGCTTCTCTTTTACAA GTATGTCTACAAGCGGTACAGGGCTGGCAAGCAGAGGGGGATGATCATTGAACATGAAGGAGACAGACCA GCTTCCAAAACTGAAATTGAAATGGATGGGAAAGTAGTCAACTCCCACGTTGACAATTTCTTAGATGGCG CTCTGGTTTTGGAAGTCGATGAGAGGGACCAAGATGACGAGGAAGCCAGGCGTGAGATGGCAAGGATTCT GAAGGAACTTAAGCAGAAGCATCCCGACAAAGAGATCGAACAATTAATAGAATTAGCCAACTATCAAGTC CTAAGTCAGCAGCAAAAGAGCCGAGCATTTTACCGAATTCAAGCTACTCGCCTGATGACTGGAGCTGGTA ACATTTTGAAGAGGCATGCAGCTGACCAAGCGAGGAAGGCTGTCAGCATGCATGAAGTCAACATGGATGT GGTTGAAAATGACCCAGTCAGTAAGGTCTTCTTTGAGCAAGGGACATACCAGTGTCTAGAAAACTGTGGT ACTGTGGCCCTCACCATTATTCGAAGAGGGGGTGACTTGACCAACACTGTGTTTGTTGACTTCAGGACGG AAGATGGCACAGCCAATGCTGGGTCTGATTATGAGTTCACGGAAGGGACTGTGATCTTCAAACCTGGGGA GACCCAGAAGGAAATCAGAGTTGGCATCATTGATGATGATATCTTTGAAGAAGATGAAAACTTTCTTGTG CATCTTAGCAACGTCAGGGTCTCTTCAGAAGTCTCGGAAGATGGCATACTAGACTCCAATCACGTGTCTG CGATTGCTTGTCTCGGGTCACCCAACACTGCCACCATAACCATTTTTGATGATGACCACGCGGGCATCTT TACTTTCGAGGAACCCGTGACTCACGTGAGCGAGAGCATTGGCATCATGGAGGTGAAGGTGCTGAGAACC TCTGGAGCGCGAGGAAATGTTATCATTCCCTATAAAACCATTGAAGGCACAGCCCGAGGTGGAGGGGAGG ACTTTGAGGACACCTGTGGAGAGCTGGAATTCCAGAATGATGAAATAGTCAAAACAATATCAGTCAAGGT AATCGATGACGAGGAGTATGAGAAAAACAAGACCTTCTTCATTGAGATTGGAGAACCCCGTCTGGTGGAG ATGAGTGAGAAGAAAGGTGGCTTCACATTAACAGAGGAGTACGATGACAAGCAGCCACTGACCAGCAAAG AGGAGGAGGAGAGGCGCATTGCAGAAATGGGGCGCCCCATTCTAGGCGAACACACCAAGCTGGAAGTGAT CATTGAAGAGTCTTACGAATTCAAGAGCACTGTGGACAAACTCATTAAGAAGACGAACCTGGCCCTCGTG GTGGGGACCAACAGCTGGAGAGAGCAGTTCATTGAAGCGATCACCGTCAGCGCTGGGGAAGATGACGATG ATGATGAATGTGGGGAGGAGAAGCTGCCCTCCTGTTTTGATTACGTGATGCACTTTCTCACAGTGTTCTG GAAGGTTCTGTTTGCCTTCGTCCCACCTACAGAATATTGGAATGGCTGGGCCTGCTTCATTGTCTCCATC CTCATGATCGGCCTACTGACAGCCTTCATTGGAGATCTGGCTTCCCACTTTGGCTGCACCATTGGTCTGA AAGATTCCGTGACTGCAGTTGTGTTTGTCGCTCTTGGAACCTCAGTGCCAGACACATTTGCCAGCAAAGT AGCAGCTACCCAGGACCAGTATGCAGATGCGTCCATAGGCAATGTCACCGGAAGCAACGCTGTGAATGTC TTCCTGGGAATCGGCGTGGCCTGGTCCATTGCTGCCATCTACCATGCGGCCAACGGGGAACAGTTCAAAG TGTCCCCTGGCACGCTAGCTTTCTCTGTCACTCTCTTCACCATTTTTGCTTTCATCAACGTGGGGGTGCT GCTGTATCGGCGGAGGCCAGAAATAGGAGGTGAGCTGGGTGGGCCCCGGACTGCCAAGCTCCTCACATCT TCCCTGTTTGTGCTCCTGTGGCTCTTGTACATTTTCTTCTCCTCCCTGGAGGCCTACTGCCACATAAAAG GCTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270772
Insert Size	2808 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270772.1, NP_001257701.1
RefSeq Size	6134 bp
RefSeq ORF	2808 bp
Locus ID	29715
UniProt ID	Q01728
Gene Summary	displays Na+ gradient-dependent Ca2+ transport activity; plays a role in regulation of calcium ion transport [RGD, Feb 2006] Transcript Variant: This variant (2) lacks three alternate in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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