Atp12a (NM_001301664) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217338 representing NM_001301664 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGGGGCTTTTCCATCCTCCTGTGGATAGGTGCTGCCTTGTGCTGGATCGCATTTGTGATCCAGT ACGTCAACAATTCCGCATCCCTAGACAACGTCTACTTGGGCGCCATACTTGTCCTGGTTGTTATTTTAAC GGGGATTTTTGCTTATTACCAAGAAGCCAAAAGCACCAACATCATGGCCAGCTTTAGTAAGATGATCCCC CAGCAAGCTCTTGTCATCCGAGATGCCGAAAAGAAGGTCATTTCAGCAGAGCAACTGGTGGTGGGGGACG TTGTGGAGATTAAAGGAGGGGACCAGATCCCTGCAGACATCAGACTGGTATTTTCCCAAGGGTGCAAAGT AGATAACTCCTCTCTCACTGGGGAATCTGAGCCCCAGGCTCGTTCAACTGAGTTTACCCATGAAAACCCC TTGGAAACCAAGAACATAGGCTTCTATTCCACAACCTGCCTGGAAGGTACAGCAACTGGCATTGTCATCA ACACGGGTGACCGTACCATCATCGGGCGCATAGCCTCCTTGGCTTCAGGCGTTGGGAGTGAGAAAACACC CATTGCCATTGAGATCGAGCATTTTGTCCATATTGTGGCAGGAGTGGCTGTGTCCATTGGCATCATTTTC TTCATCACCGCCGTGTGCATGAAGTACTATGTCCTCGACGCCATCATCTTCCTCATTAGCATCATTGTGG CTAACGTGCCAGAGGGCCTCCTGGCTACTGTCACTGTTACCCTGTCACTGACGGCAAAACGGATGGCCAA GAAGAACTGCCTGGTAAAGAATCTGGAGGCAGTGGAGACTCTTGGCTCCACCTCCATCATCTGCTCAGAC AAGACGGGGACCCTGACCCAGAACAGGATGACTGTGGCTCACTTGTGGTTTGACAATCAGATCTTTGTGG CTGATACTAGTGAAAACCAAACAAAACAAGCCTTTGACCAAAGCTCTGGAACCTGGGCTTCCTTGTCCAA GATAATAACACTGTGTAACCGAGCAGAGTTCAGGCCAGGCCAGGAGAGTGTCCCCATCATGAAGAGAACT GTGGTTGGAGACGCCTCAGAAACTGCTCTGCTAAAGTTCTCAGAGGTCATTTTGGGTGACGTGATGGGAA TTAGGAAAAGAAACCACAAAGTGGCTGAAATTCCTTTTAATTCTACCAACAAATTTCAGCTCTCCATACA TGAGACAGAGGACCCTAATGACAAACGCTTCCTCGTGGTGATGAAAGGAGCCCCTGAGAGGATCTTAGAG AAGTGCAGCACCATCATGATCAATGGCCAGGAGCAGCCACTGGACAAGAGCTCTGCTGATAGCTTCCACA CAGCCTACATGGAGCTGGGCGGTCTGGGCGAGCGTGTGTTGGGTTTCTGTCACCTCTACTTGCCAGCAGA GCAGTTCCCCCAAAGTTACATCTTTGATGTCGACTCCGTAAACTTTCCCACTTCCAACTTCTGCTTTGTG GGGCTTCTGTCCATGATCGACCCTCCTCGGTCTACTGTGCCAGACGCAGTCTCCAAATGTCGGAGTGCCG GGATCAAGGTTATCATGGTCACAGGTGATCACCCTATCACCGCCAAAGCTATTGCCAAGAGTGTAGGAAT CATATCGGCCAACAACGAGACAGTGGAAGACATCGCGAAACGCCGCAATATCGCTGTGGAGCAGGTCAAC AAAAGAGAAGCTAAAGCCGCTGTGGTGACTGGCATGGAGCTGAAGGACATGACCCCAGAACAACTGGATG AGCTTTTGACCAACTACCAAGAAATCGTATTTGCCCGGACATCACCCCAGCAGAAGTTGATCATTGTGGA GGGCTGTCAGAGGCAGGACGCCATCGTTGCCGTGACGGGTGATGGAGTGAACGACTCTCCAGCACTGAAG AAGGCAGACATCGGGATTGCTATGGGGATAGCAGGTTCTGACGCTGCTAAGAACGCAGCTGACATGGTCT TACTAGACGACAACTTCGCGTCTATAGTCACAGGGGTGGAGGAAGGTCGCCTGATCTTCGACAACCTGAA GAAAACCATTGCGTACACCCTGACAAAGAACATCGCCGAGCTCTGCCCTTTTTTGATCTACATCGTTGCT GGGCTCCCCCTGCCTATTGGAACCATCACTATCCTATTCATCGACCTGGGCACAGACATTATCCCCTCCA TTGCCTTGGCCTATGAGAAAGCAGAAAGTGACATTATGAATAGGAAACCTCGCCACAAGAAGAAAGACAG ACTGGTGAACACGCAGCTGGCCATCTACTCTTACCTTCACATTGGTCTCATGCAAGCTCTGGGAGGTTTC CTCGTCTATTTCACTGTCTATGCACAGCAGGGCTTTTGGCCCACTTCTCTCATCAACCTGAGGGTAGCAT GGGAAACAGATGACATAAATGACTTGGAAGACAGCTACGGGCAGGAATGGACAAGGTATCAGAGAAAATA CCTGGAATGGACAGGCTCAACGGCTTTCTTTGTTGCCATCATGATCCAACAAATTGCGGATCTGATCATC AGGAAAACCCGGAGGAATTCCATCTTTCAGCAGGGGCTCTTCAGAAATAAAGTCATCTGGGTGGGGATCG CCTCACAGGTCATCGTTGCACTGATCCTCTCCTATGGTCTCGGGAGTGTCCCAGCTCTGAGCTTTACCAT GCTCAGGGTTCAGTACTGGTTTGTGGCTGTGCCACATGCCATTTTGATTTGGGTGTATGACGAGATGCGA AAGTTGTTCATCAGGCTCTACCCTGGAAGCTGGTGGGATAAGAACATGTATTATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301664
Insert Size	2787 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301664.1, NP_001288593.1
RefSeq Size	4160 bp
RefSeq ORF	2787 bp
Locus ID	171028
Gene Summary	may act as a H+,K(+)-ATPase in distal colon; may also play a role in K+ absorption and H+ secretion in the distal nephron [RGD, Feb 2006] Transcript Variant: This variant (2) has an alternate 5' sequence, which results in translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (b, also known as HKalpha2b) has a shorter N-terminus, compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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