Ddr1 (NM_001166022) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cak; Drd1; PTK3D
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217313 representing NM_001166022 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACAGGGACCCTCTCATCTCTCCTGCTGCTGCTACTCTTGGTGACAATTGGAGATGCTGACATGA AGGGACATTTTGACCCAGCCAAGTGCCGCTATGCCCTGGGCATGCAGGACCGCACCATTCCAGACAGCGA TATCTCTGTGTCCAGCTCCTGGTCGGACTCTACCGCCGCCCGCCACAGCAGGCTGGAAAGCAGTGATGGA GATGGGGCTTGGTGCCCTGCAGGGCCTGTGTTCCCCAAAGAAGAGGAGTACCTGCAGGTGGACCTTCGTA GGCTACACCTGGTGGCTCTGGTGGGCACCCAGGGCCGCCATGCTGGGGGTCTGGGCAAAGAGTTCTCCCG AAGCTACCGGTTGCGTTACTCCCGAGATGGCCGACGATGGATGGACTGGAAAGACAGATGGGGTCAGGAG GTGATTTCGGGTAACGAGGATCCTGGGGGAGTGGTGCTGAAGGATCTCGGGCCCCCCATGGTGGCCCGGC TGGTCCGTTTCTACCCCAGGGCTGACCGGGTCATGAGTGTCTGTCTTCGGGTGGAGCTCTATGGCTGCCT CTGGCGGGACGGACTCTTGTCCTATACAGCCCCCGTGGGGCAGACCATGCAATTATCTGAGATGGTGTAT CTCAATGATTCCACTTACGATGGATATACTGCTGGAGGGCTGCAGTACGGTGGTCTGGGCCAACTGGCAG ACGGCGTGGTGGGGCTGGATGATTTCAGGCAGAGCCAGGAGCTGCGGGTTTGGCCAGGCTATGACTATGT GGGATGGAGCAACCATAGCTTCCCCAGCGGCTACGTGGAGATGGAGTTTGAGTTTGACCGGCTGAGGTCT TTCCAGACCATGCAGGTCCACTGTAACAACATGCACACTCTGGGAGCCCGCCTCCCAGGCGGGGTGGAAT GCCGGTTTAAAAGGGGTCCGGCCATGGCCTGGGAAGGAGAGCCTGTACGCCATGCCCTGGGGGGTAGCCT TGGAGACCCCAGAGCCAGGGCCATCTCGGTGCCCCTGGGTGGTCACGTGGGCCGCTTCCTGCAGTGCAGA TTCCTCTTTGCAGGACCCTGGTTACTCTTCAGTGAGATCTCTTTCATCTCGGATGTGGTGAACGACTCGT CTGACACCTTCCCACCAGCCCCCTGGTGGCCGCCTGGCCCGCCTCCCACCAACTTCAGCAGCTTGGAGCT GGAGCCCCGGGGTCAACAGCCTGTGGCCAAGGCGGAGGGGAGCCCAACTGCCATCCTCATTGGCTGCCTG GTGGCCATCATCCTTCTCCTCCTCCTTATCATTGCCCTGATGCTCTGGAGGCTGCACTGGCGCCGGCTGC TCAGCAAGGCAGAGCGCCGGGTGTTGGAGGAGGAGCTGACGGTTCACCTCTCTGTCCCTGGGGACACCAT CCTCATCAACAACCGCCCAGGGCCCCGAGAGCCACCCCCTTACCAGGAACCCCGGCCTCGGGGGACTCCA ACTCACTCTGCGCCCTGCGTCCCCAATGGCTCTGCGTTGCTGCTCTCCAATCCGGCCTACCGCCTCCTTC TGGCCACTTACGCCCGTCCCCCTCGAGGCCCGGGCCCCCCCACACCCGCCTGGGCCAAACCCACCAACAC CCAGGCCTGCAGTGGGGACTACATGGAGCCCGAGAAGCCGGGCGCCCCACTTCTACCCCCACCTCCCCAG AACAGCGTCCCCCATTACGCCGAGGCTGACATTGTCACCCTGCAGGGCGTCACAGGGGGCAACACCTACG CTGTTCCCGCACTGCCCCCAGGGGCGGTTGGGGATGGGCCCCCCAGAGTGGATTTCCCTCGGTCACGGCT CCGTTTCAAGGAGAAGCTCGGCGAGGGCCAATTTGGGGAGGTACATCTCTGTGAAGTAGAGGACCCTCAA GATCTGGTCACTAGTGACTTCCCTATCAGTGTGCAAAAGGGACACCCCTTGCTGGTGGCAGTGAAGATCC TCCGGCCAGATGCCACCAAAAATGCCAGGAATGACTTCCTGAAGGAGGTAAAGATCATGTCACGGCTGAA GGACCCAAACATCATCCGGCTCCTGGGTGTGTGTGTGCAGGATGACCCTCTCTGCATGATTACAGACTAC ATGGAGAACGGTGACCTGAACCAATTCCTCAGTGCCCACCAGCTGGAGAACAAGGTCACTCAGGGGCTTC CTGGGGACAGAGAGTCTGACCAGGGGCCCACAATCAGCTACCCTATGCTGTTACACGTGGGGGCCCAGAT CGCCTCTGGCATGCGATATCTGGCCACCCTTAACTTTGTGCATCGGGACCTGGCCACCCGGAACTGCTTG GTTGGGGAAAATTTCACCATCAAAATCGCCGACTTTGGCATGAGCCGGAATCTCTACGCTGGGGATTATT ACCGTGTACAGGGCCGGGCAGTGCTGCCCATCAGGTGGATGGCCTGGGAGTGTATCCTCATGGGGAAGTT CACAACAGCCAGTGATGTGTGGGCCTTCGGAGTGACCCTGTGGGAGGTGCTTATGCTCTGCAGGTCACAG CCCTTCGGGCAACTCACAGACGAGCAGGTCATCGAGAACGCCGGCGAGTTCTTCAGGGACCAGGGCCGGC AGGTATACTTATCCAGGCCACCCGCCTGCCCGCAGACCTTGTATGAGCTGATGCTCCGGTGTTGGAGCCG GGAGCCGGAGCAGCGGCCGCCCTTCTCCCAGCTTCATCGGTTCCTGGCGGATGATGCACTCAACACAGTG TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001166022
Insert Size	2733 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001166022.1, NP_001159494.1
RefSeq Size	2834 bp
RefSeq ORF	2733 bp
Locus ID	25678
Gene Summary	protein tyrosine kinase that may play a role in nervous system development [RGD, Feb 2006] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were based on transcript alignments.

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