Kctd1 (NM_001100516) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Vad; Vad5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217273 representing NM_001100516 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGAATGCCTGGCAGCGGGGACTGTAACACCAGCGCGGGCGGCAGCGCCGCCGCTGGGGCCGAGA ACAATGGGGAGCGGGGCGAGGGCGAGCGCGGGGCCGGGGGCCGCGGTCGCCGCTACGGCCGCCCGCATTA CTGCAGCGCGGGCGAGGAGGAGGAGGAAGAGGAGGAGGAGGACGAGATCCAGGAGGTGCAGATAACGGGG GACGAGGAGGAGGACGGAGGTGGGGGGCTGGAGGAGGACGAGGAGGAGGAGGAAGAGGAGGAGATGGGGA TGGACTGGGAAGAGCCCCTGGAGCCAGAGGACTCGGCCGGGGAGGAGCTGGAGCCCGAGCCGGTCCATAT GATCCATATGGACCAGAGCGCCGCGCTGGAGCCCGAGGCGCCGCCACGACTGCTGGCGCCCCGGGCCCGC GCGGGGCCGCCCGGGGACAGCGCGGAGCTGGACCCCGACGTGCTGCAGCGCCCCGAGCGGGCCCGGCTGA GCGAGAACACCCGGCTGGCCACCCGCTACGCCGTGCGCATCTTCCGGGAGTACCTGAGCGAAAAGGCTCA GAGCCCAGACTTCGAGACCATGGACAAGGGGGCGCTGTGCCGCGTGCTGCGCTCCTTCTATGCCGAGGCC CGTTCTAAGAGCGGCCAGCTCTACAGCAAGTCCTCGCTCATCAGCATCCGCAGCTCCCTCAACCGCTACC TCAACGAGCCCCCTTACTGTCGCACGCTCGACCTCACCAAGGACCCCGAGCTGCGCAGCGCCAACCTGAC GCTGGCCGCAGTCATCCGCAAGCTCGAGGAGCAGGGCGCGGGGCCCGTGGTGCAGAAGCAAGCCATCACG CGCGCCGACCTGCGCAAGCTCTACACCTCCAGCGTCTTCAGCACCAACACGCCCTTTGGACTGCTCAACA AGGTCTGGTTCGAGACGTGCATGTACTTTTGCACGCGAGGCCGCGAGAACCAGCGCGAGCTGGAGGAGGA CTCCTTCGGGCTGGCCATGGACGAGGACGGTCGCAAGTTCGTCTACTTCAAGTCGCTCGGGCCTTACCAC AAGTCGCGTTCCTCGTCCTGGAGTAAGAAGCGAGCGGAGAGCAGCGACGAAGAGAACTTGCCTCGCATGT ACGAGACAGGCACTGAGTTCTGTCCCTACGCCAGCTTTGTCAAGTACCTGTCTAAGCGCAATCCCCTCTG TAAGGCGTTCTTCCAGCGGCCCCGGGACCACTGCAGCGAGGGCGATGTGACATGGTACGAGAACAAAGCC ATAGGCAAGAACTTGCTGGGCACCAGGATGCAGATGCTCTCCAAGGCAGCCAAGCTGTCCAAGACCTACA CTAACCACTGCATCGGCGCGGTGTCCATCGCCACGCTCAACAGCATCGCGGGCATCGGGACCAAGCTAGG CTCCCCAGCCCCGCAGGGCTGCTACACCGACTCTCTGAACGGGTCAGCTCGTCACCACTCCCACCATGCC CCCACCCATCCTTCCCACCACCACCGCCCCCAGCCGTCCTCGCTGGGTAGCACTTATATCCTCCCCAAAG ACAGCCAGGTCGGGCCCGACGTGAAGTCCGAGGCTGCGCCCAAGCGCGCGCTGTACGAGTCAGTGTTCGG GTCCGGGGAAATCTGCGGCCCTTCTTCCCCCAAAAGACTTTGTATCCGTCCCTCCTCGGAACCTGTGGAT GCGGTGGTAGTGGTTTCCGTGAAACACGACCCCCTGCCTCTTCTTCCAGAAGTCAATGGGCACCGAAGCA CCAATTCTCCCACCATAGTTTCACCTGCTATTGTTTCCCCCACCCAGGACAGTCGGCCCAATATGTCAAG ACCTCTGATCACTAGATCCCCCGCATCGCCACTGAACAACCAAGGCATTCCAACTCCAGCACAGCTCACC AAGTCCAATGCTCCCGTCCACATTGACGTGGGCGGCCACATGTACACCAGCAGCCTGGCCACCCTCACCA AATACCCCGAATCAAGAATCGGAAGACTCTTCGATGGCACAGAGCCCATTGTCCTGGACAGTCTCAAACA GCACTATTTCATTGACAGAGACGGGCAGATGTTCAGATATATCTTGAATTTTCTACGAACATCAAAACTC CTCATTCCCGATGATTTCAAGGACTACACTTTGTTGTATGAAGAGGCAAAATATTTCCAACTCCAGCCCA TGTTGTTGGAGATGGAGCGATGGAAACAGGACAGAGAAACGAGCCGCTTTTCACGGCCCTGCGAGTGCCT CGTGGTGCGTGTGGCCCCGGACCTTGGAGAAAGGATCACGCTCAGTGGGGACAAGTCCTTGATAGAAGAA GTGTTTCCCGAGATCGGCGACGTGATGTGCAACTCCGTCAATGCAGGTTGGAATCACGACTCGACGCATG TCATCAGGTTTCCACTAAACGGCTACTGCCACCTCAACTCAGTTCAGGTCCTCGAGCGGTTGCAGCAAAG AGGATTTGAAATTGTGGGTTCCTGTGGAGGAGGCGTGGACTCATCCCAGTTCAGTGAATACGTCCTCCGA CGGGAACTGAGACGGACACCCCGAGTACCCTCCGTCATCCGGATAAAACAGGAGCCACTGGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100516
Insert Size	2586 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100516.1, NP_001093986.1
RefSeq Size	3431 bp
RefSeq ORF	2586 bp
Locus ID	291772

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