Piwil4 (NM_001271133) Rat Untagged Clone

CAT#: RN217245

Piwil4 (untagged) - Rat piwi-like RNA-mediated gene silencing 4 (Piwil4)



  "NM_001271133" in other vectors (1)

CNY 7,980.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN217245 representing NM_001271133
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGGACGGGCCCGTGTGAGGGCGCGAGGCATCACCACTGGCCACAGTGCCAGGGAAGTGGAGCGGG
CCCCAAGAGACCTCATGGTGAGTGCTTCTGTCTATGAGCTTGGCGTCTCTTCTGGTGATGGTAGACGTAC
TCTCGTGGAAAAAAGAGGCAATGGCAGACAGGACTTCGTCGATTTGGGTGTCTGTACCAGGGAGAAGTTG
ACTCACGTAAAAGATTCTAAAACAGGTTCTAGCGGAATACCTGTGCAGCTGGCTACAAATCTCTTCCATT
TAGATTTGCCCCAGGATTGGCAGCTATACCAGTACCATGTCACATACAGCCCGGATTTAGCCTCTAGGAG
GCTGAGGACTGCTCTGCTGTATAACCACAGCATACTCTCCGACAAGGCAAAGGCATTCGACGGCACCAGC
CTTTTCCTCTCAGAAAAGCTGGACCACAAAGTCACAGAACTGACAAGTGAAACCCAAAGAGGGGAGACTG
TGAAGATAACTCTCACCCTGACCAGTGAGCTGTTCCCAAACTCCCCGATGTGCATTCAGTTTTTCAATGT
CATCTTCAGAAAGATCTTTAAAAAGTTGAGCATGTACCAGATTGGACGGAACTACTATAAGCCTTCAGAG
CCAGTGGAGATCCCCCAGTACAAATTGTCCCTGTGGCCTGGGTTTGCCATTTCTGTGTCTCGCTTTGAAA
GCAAACTCCTGTTCAATGCTGACGTAAACTATAAAGTCCTCCGCAATGAGACTGTTCTGGAATTCATGAC
TGATCTCTGCCTCAGAACCGACATGTCATGCTTCACTGAGACGTGCCACAAACAGCTAGTGGGGCTCATT
GTCCTTACCAGGTACAACAACAAAACATATCGTATCGATGACATTGACTGGTCTGTGAAGCCCACACATG
CCTTTCAGAAGCGGGATGGCTCAGAGACCACGTATGTGGATTACTACAAGCAGCAATATGATATCACCTT
ATCTGACCTAAATCAGCCAGTGCTTGTCAGTCTGTTGAAAAGAAGGAGAAATGATAACTCCGAGCCTCAG
ATGGTCCACCTGATCCCGGAGCTCTGCTTTCTCACGGGCCTGACCAGCCAAGCAACCTCAGACTTCCGCT
TGATGAAGGCAGTCGCTGAAGAAACTCGGCTCAGTCCTATGGGAAGGCAGCAGCAACTAGCCCGACTGGC
AGATGACATCCAGAGAAACCAAGTGACTCGGTTTGAGCTTGAGACCTGGGGATTGCATTTTGGAAGCCAG
CTATCCCTGACCGGCCGGGTTGTTCCCTCTGAAAAGATCCTGCTGCAGGACCACACATGTCAACCTGCAT
TTGCTGCTGATTGGTCCAAAGATATGCGATCTTGCAAGGTTCTGAGTTCTGAGCCTTTGAATACATGGTT
GATCGTGTGCTGTAGCAGGGCTGAGCATTTGATTGAATCCTTCCTGAGCTGTCTGAGGAGAGTTGGAGGT
TCCACGGGATTCAGTGTGGGCCACCCAAAAATCATAAAAGTGGATGAAAACCCTGCAGCGTTCCTTCGAG
CCATCCAGCTGCACGTCAACCCCGATGTTCAGTTGGTGATGTGCATCCTGCCTTCTAATCAGAAGAACTA
TTACGACTCCATTAAAAGATATTTGAGCTCCGACTGCCCGGTCCCAAGCCAGTGTGTGCTGACCCGGACC
TTGAACAAACAGGGAATGATGCTGAGTGTGGCCACCAAGATCGCCATGCAAATGACCTGCAAACTCGGGG
GCGAGCTGTGGGCTGTGGAGATCCCATTGAAGTCACTGATGGTCGTGGGCATCGACATCTGTAGAGACGC
CCTCAGCAAGGATGTAGCGGTCGTCGGGTTTGTCGCCAGCATTAACTCCAGGATCACCAGGTGGTTTTCC
CGCTGTGTCCTCCAGAGAACGGCAGCTGATATTGCAGATTGCCTGAAAGTCTGCATGACTGGTGCTCTCA
ACAAGTGGTACAAACACAACCACGATTTGCCGGCACGGATAATCGCGTACCGGGATGGTGTGGGCAACGG
CCAGCTAAAGGCAGTTTTGGAATACGAAGTCCCACAGCTGCTGAGCAGTGTGACAGAATGTGGCTCCGAC
GCCAGGAGCTGTAGACTGTCAGTGGTCGTGGTCAGGAAGAGGTGTCTGCTGCGTCTCTTTGCCGAGTCTG
GCCATACGCTACAGAACCCCCCACTTGGCACTGTCGTGGACTCAGAAGCAACACGTCCAGAGTGGTATGA
CTTCTACCTGACCAGCCAGACTGCTAACCGGGGGACTGTTAGTCCCACCTACTACAATGTCATCTATGAC
GACAACGCTCTGAAGCCTGACCATATGCAGCGGCTGACCTTCAAACTGTGCCACCTCTACTACAACTGGC
AGGGCGTAATCAGTGTCCCTGCTCCGTGCCAGTATGCACACAAGCTGACCTTCCTGGTGGCACAAAGCAT
CCACAAGGAACCAAGTCTGGAATTAGCCAATAAGCTCTTCTACCTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001271133
Insert Size 2499 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001271133.1, NP_001258062.1
RefSeq Size 3109 bp
RefSeq ORF 2499 bp
Locus ID 689972
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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