Nfatc1 (NM_001244933) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217241 representing NM_001244933 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAAGTACCAGCTTTCCAGTCCCTTCCAAGTTTCCACTCGGCCCTCCGGCCGCAGTCTGCGGGAGCG GAGAAACTTTGCGGCCCGCGCCGCTCGCCGGCGGCACCATGAAGGCGGCCGAGGAAGAACACTACAGTTA TGCGTCCCCTAGCGTCACCTCAACTCTGCCCCTTCCCACAGCACACTCCGCCTTGCCAGCGGCATGCCAT GACCTCCAGACGTCCACCCCGGGCATCTCAGCTGTTCCTTCGGCCAATCACCCCTCTGGTTATGGGGGCG CTGTGGACAGTGGGCCTTCAGGATACTTCCTGTCCTCCGGCAACACGAGACCCAATGGGGCCCCGACTCT CGAGAGTCCTCGAATCGAGATCACCTCCTATCTGGGCCTTCACCACGGCAGCAACCAATTTTTCCACGAT GTGGAGGTGGAAGACGTACTTCCTAGCTGCAAGCGGTCACCATCTACAGCAACCCTGCACCTGCCCAGTC TGGAAGCCTACAGAGACCCCTCCTGCCTGAGCCCAGCCAGCAGCCTGTCCTCCAGGAGCTGTAACTCTGA GGCGTCCTCCTATGAGTCCAACTATTCATACCCGTACGCATCCCCCCAGACCTCTCCATGGCAGTCACCC TGTGTGTCTCCCAAGACCACGGACCCAGAGGAGGGCTTTCCCCGAAGCCTGGGTGCCTGCCACTTGCTGG GATCGCCAAGGCACTCCCCATCCACCTCTCCTCGTGCAAGCGTCACCGAGGAGAGCTGGCTCGGTGCCCG TGGCTCCCGGCCCACATCCCCCTGCAACAAGCGCAAATACAGTCTCAACGGCAGGCAGCCCTCCTGCTCA CCCCACCACTCGCCCACACCATCCCCCCATGGCTCCCCTCGGGTCAGCGTGACGGAAGACACCTGGCTTG GTAACACCACCCAGTACACCAGCTCTGCTATTGTGGCAGCCATCAACGCCCTGACCACTGATAGCACTCT GGACCTGGGTGACGGTGTCTCTATCAAGTCTCGAAAGACAGCCCTGGAGCACGTGCCCTCTGTGGCCCTC AAGGTAGAGCCGGCTGGGGAAGACCTGGGCACCACACCACCCACTTCTGACTTCCCACCCGAGGAGTTCG CCTTCCAGCACCTTCGGAAGGGTGCCTTTTGTGAGCAGTATCTGTCGGTGCCACAGGCCTCGTATCAGTG GGCAAAGCCCAAGTCTCTATCCCCAACGTCATACATGAGCCCATCCCTGCCTGCCCTTGACTGGCAGCTG CCGTCGCACTCTGGTCCGTATGAACTCCGGATCGAGGTGCAACCCAAGTCTCACCACAGGGCTCACTATG AGACGGAAGGCAGCCGGGGAGCTGTGAAGGCTTCTGCTGGAGGACACCCTGTCGTGCAGCTACATGGTTA CTTGGAAAACGAGCCTCTCACGCTCCAGCTGTTCATTGGGACGGCTGACGACCGCCTGCTACGGCCCCAT GCCTTCTACCAGGTCCACCGGATCACGGGGAAGACTGTCTCCACCACCAGCCACGAGGCTATCCTGTCCA ACACCAAAGTCCTGGAGATCCCACTGCTTCCAGAAAATAACATGCGAGCCATCATCGACTGTGCTGGGAT CCTGAAGCTCAGAAACTCTGATATCGAGCTGAGGAAAGGGGAGACAGACATCGGGAGAAAGAACACCAGG GTGCGGCTGGTCTTCCGAGTCCACATCCCCCAGCCCAGCGGCCGGACGCTGTCTCTCCAGGTGGCCTCAA ACCCGATCGAGTGCTCCCAGCGGTCGGCCCAGGAACTGCCCCTCGTGGAGAAGCAGAGCACAGACAGCTA CCCGGTCATTGGAGGGAAGAAGATGGTGTTGTCTGGCCATAACTTTCTGCAAGACTCCAAGGTCATCTTC GTGGAGAAGGCCCCAGATGGCCACCATGTCTGGGAGATGGAAGCGAAAACTGACCGAGACCTGTGCAAGC CAAATTCCCTGGTGCTGGAGATACCACCTTTCCGCAACCAGAGAATAACCAGCCCCGTCCAAGTCAGCTT CTATGTCTGCAACGGGAAGCGGAAGAGAAGCCAGTACCAGCATTTCACGTACCTTCCTGCCAATGTTCCA ATTATAAAGACAGAACCCACGGACGACTTTGAGCCAGCTCTGACCTGTGGACCAATGAGCCAGGGACTTA GCCCTCTGCCGAGGCCTTACTATAGCCAACAACTCACCGCGCCTCCTGACCCTGGCTCCTGCCTTGTGGC CGGCTTTGCCCCCTGCTCACAGAGGAACACGCCGATGCCCACGTCTCCCAATGCAAGCCCAAAGCTCCAC GACCTTTCCTCTCCCGCCTACGCCAAGGGCCTCGCCAACCCAGGCCACAGTGGTCACCTTGGACTTCAGC CACCAGCTTCAGAGGCCCCCACCATGCAGGAATTGCCGAGACCCATGGCCGTCCAGCCCAACTCGCCTGA GCAGCCCACATCCGTCAGGCTACAGCCGCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001244933
Insert Size	2484 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001244933.1, NP_001231862.1
RefSeq Size	4132 bp
RefSeq ORF	2484 bp
Locus ID	100361818

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