Sema4b (NM_001170462) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217234 representing NM_001170462 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCGGGCGAGCCGCTCCGCCGACCTGCTGAGCGCGCTGCAGCTGCTGTTGCTACTGCTGCGGACGA CGACGGCGCGGGCGCTCGGCCCACGGATCAGTGTGCCGCTGGACTCTGAAGAAAGGCTGATTAGAAAATT TGAAGCTGAAAACATCTCCAACTACACGGCCCTTCTGCTGAGCCAAGATGGCAAGACGCTGTATGTGGGG GCCCGAGAGGCCCTCTTTGCCCTTAACAGCAACCTCAGCTTCTTGCCGGGCGGGGAGTACCAAGAGCTGC TGTGGAGTGCAGATCCTGACAGGAAGCAGCAGTGCAGCTTCAAGGGCAAGGACCCAAAGCGTGACTGTCA AAACTACATCAAGATCCTTCTGCCCCTCAACAGCAGCCATCTGCTCACCTGTGGCACCGCAGCCTTCAGC CCCCTGTGTGCTTACATTAACACAGCAAGCTTTACATTAGCCCGAGACGAGGCTGGCAGTGTCCTCCTAG AGGATGGCAAGGGCCGGTGTCCCTTTGACCCCAACTTCAAGTCCACGGCTCTGGTGGTTGATGGTGAGCT GTACACTGGAACAGTCAGTAGCTTCCAGGGAAACGACCCAGCCATTTCCCGGAGCCAGAGTCCCCGCCCC ACCAAGACTGAGAGCTCCCTCAACTGGCTGCAAGACCCTGCCTTTGTGGCCTCAGCCTATGTTCCTGAGA GCCTGGGCAGCCCAATAGGTGATGATGATAAGATCTACTTCTTCTTCAGCGAGACGGGCCAGGAGTTTGA GTTCTTTGAGAACACCATTGTCTCCAGAGTTGCCCGAGTCTGTAAGGGCGATGAGGGTGGAGAGCGGGTG TTGCAGCAGCGCTGGACTTCCTTCCTCAAGGCTCAGCTCCTGTGCTCCCGGCCCGATGATGGCTTTCCCT TTAACGTGCTACAAGATGTCTTCACCCTGAACCCCAACCCTCAGGACTGGCGCAAGACCCTTTTCTATGG GGTCTTTACCTCCCAGTGGCACAGAGGGACCACAGAAGGCTCTGCCATCTGCGTCTTCACCATGAATGAC GTGCAGAAGGCCTTTGATGGTCTGTATAAGAAAGTGAACAGAGAGACACAGCAGTGGTATACTGAGACCC ACCCAGTGCCCACTCCCCGGCCGGGAGCGTGTATCACCAACAGTGCCCGGGAACGGAAGATCAACTCGTC GCTGCAGCTCCCGGACCGAGTGCTGAACTTCCTCAAGGATCACTTCTTGATGGATGGGCAGGTCCGCAGC CGCCTGCTGCTGCTCCAGCCACAAGCCCGCTACCAGCGTGTGGCCGTGCACCGAGTGCCTGGCCTGCACA GCACGTATGATGTACTATTTCTGGGCACTGGTGATGGTCGCCTGCACAAGGCAGTGACCCTGGACTCCAG AGTGCACATCATTGAGGAGCTGCAGGTCTTCCCTCAAGGACAGCCTGTGCAGAACCTGCTCTTGGACAGC CATGGGGGACTGTTGTATGCCTCCTCCCATTCTGGGGTAGTGCAAGTGCCCGTAGCCAACTGCAGCCTGT ACCCAACCTGTGGAGACTGCCTCCTGGCTCGAGACCCTTACTGCGCCTGGAACGGCTCCACCTGCAAGCT CCTTAGTCTCTACCAGCCTGATCTGGCCTCCAGGCCATGGATCCAGGATATTGAGGGTGCCAATACCAAG GAACTCTGCAATATTTCCTCAGCCAAGGCCCGGTCTTTTAAGCCAGGTAAGCCATGTAAACAAGTCCAGA TCCAAGCAAACACAGTGAACACCCTGGCCTGCCCACTCCTCTCAAACCTGGCTACTCGGCTCTGGCTGCA CAATGGGGCTCCTGTCAATGCTTCTGCCTCCTGCCGGGTGTTACCCAGTGGGGACCTGCTGCTGGTAGGC AGCCAGCAGGGCTTGGGGGTGTTCCAGTGTTGGTCCATAGAAGAAGGATTCCGGCAGCTGGTGGCCAGCT ACTGCCCAGAGGTGAAGGAGGATGGAGTAATGGACCAAAATGACCAGAGTGATGGCACCCCGGTCATTAT CAACACATCACGAGTGAGTGCACCTGCTGGTGGCAGGGCCAGCTGGGGTGCAGACAAGTCATACTGGAAT GAATTCCTGGTGATGTGTACTCTGTTTGGGCTTGCTGTGGTGCTTCTGGTTCTGTTCTTTCTCTACCGAA ATCGGGATGGCATGAAGCTCTTCCTAAAGCAGGGCGAATGTGCCAGTGTGCACCCCAAGACTCGCCCTGC AGTGCTCCCACCTGAGACCCGACCACTCAATGGTGTTGGCCCTCCCAGCACCCCCCTTGACCACCGAGGC TACCAGGCTCTGTCGGATAGCTCCCCAGGGCCCCGAGTCTTTACTGAGTCAGAGAAGAGGCCACTGAGTA TCCAGGACAGCTTTGTGGAGGTGTCTCCTGTGTGTCCCCGGCCCCGAGTTCGACTGGGCTCTGAGATCCG AGACTCTGTGGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170462
Insert Size	2466 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001170462.1, NP_001163933.1
RefSeq Size	3449 bp
RefSeq ORF	2466 bp
Locus ID	293042

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