Pla2g6 (NM_001270796) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	iPla2; PNPLA9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217171 representing NM_001270796 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTTCTTTGGACGCCTCGTTAACACCCTCAGTAGTGTCACCAACTTGTTCTCAAACCCATTCCGGG TAAAGGAGGTGTCCTTGGCTGACTATGCCTCAAGTGAACGTGTTCGAGAGGAAGGGCAGCTGATCCTGTT GCAGAATGCTTCCAATCGTACCTGGGACTGTGTCCTGGTCAGCCCTAGGAACCCACAGAGTGGCTTCCGA CTCTTCCAACTGGAGTCAGAGGCAGACGCCCTGGTGAACTTCCAGCAGTATTCCTCCCAGCTGCCACCGT TCTATGAGAGCTCTGTGCAGGTCCTGCATGTGGAGGTGCTGCAGCACCTGACGGACCTCATTCGGAACCA CCCCAGCTGGACAGTGACGCACCTGGCCGTGGAACTTGGCATTCGGGAGTGTTTCCATCACAGCCGCATC ATCAGCTGCGCCAACAGCACAGAGAATGAGGAGGGCTGTACCCCACTACATCTGGCCTGCCGCAAGGGTG ACAGTGAGATTCTGGTGGAGTTGGTACAGTACTGCCACGCCCAGATGGATGTCACTGACAACAAAGGAGA GACCGCCTTCCATTATGCTGTGCAAGGGGACAATCCCCAGGTGCTACAGCTCCTAGGAAAGAATGCCTCA GCCGGCCTGAACCAGGTGAACAACCAAGGGCTGACGCCGCTGCACCTGGCCTGCCAGATGGGGAAGCAGG AGATGGTGCGTGTCCTGCTGCTCTGCAATGCCCGCTGCAACATCATGGGGCCCGGTGGCTTCCCCATCCA CACAGCCATGAAGTTTTCCCAGAAGGGGTGTGCTGAAATGATTATCAGCATGGACAGTAACCAGATCCAC AGCAAGGATCCTCGCTATGGAGCCAGCCCGCTCCACTGGGCCAAGAACGCTGAGATGGCCCGAATGCTTC TGAAGAGGGGCTGTGACGTGGACAGCACAAGTGCCTCGGGGAACACAGCCCTGCACGTGGCGGTGACGCG CAACCGCTTTGACTGTGTCATGGTGCTGCTGACCTATGGGGCCAACGCAGGTGCCCGAGGAGAGCATGGG AACACACCACTGCATCTGGCCATGTCGAAAGATAACATGGAGATGGTCAAAGCCCTCATTGTATTTGGGG CAGAAGTAGACACCCCGAATGACTTTGGGGAGACTCCTGCATTCATAGCTTCCAAGATCAGCAAGCAGCT TCAGGACCTCATGCCCGTCTCCCGAGCCCGGAAGCCAGCGTTCATCCTGAGCTCCATGAGGGATGAGAAG CGAAGCCATGACCACCTGCTCTGCTTGGATGGAGGGGGCGTGAAGGGCCTGGTCATCATCCAGCTTCTCA TTGCCATTGAGAAGGCCTCAGGTGTGGCTACCAAGGACCTCTTCGACTGGGTGGCAGGAACCAGCACAGG CGGCATCCTGGCCCTGGCCATTCTACACAGTAAATCCATGGCCTATATGCGTGGCGTGTACTTCCGTATG AAGGATGAGGTGTTTCGGGGCTCACGGCCCTATGAGTCTGGGCCCCTGGAGGAGTTCCTGAAGCGTGAGT TTGGGGAGCACACCAAGATGACAGATGTCAAGAAACCCAAGGTGATGCTGACAGGGACACTGTCAGACCG GCAGCCAGCAGAGCTCCACCTCTTCCGGAATTACGACGCCCCAGAGGCCGTTCGGGAACCTCGCTGTACC CCAAACATTAACCTGAAGCCGCCCACCCAGCCTGCAGACCAGTTAGTATGGCGGGCAGCCCGGAGCAGTG GGGCAGCCCCAACCTATTTCCGGCCCAATGGACGCTTCTTGGATGGTGGGCTGCTGGCCAACAACCCCAC ACTGGATGCCATGACTGAAATCCATGAGTACAATCAGGATATGATCCGCAAGGGCCAGGGCAACAAGGTG AAGAAACTCTCCATAGTCGTGTCTCTGGGGACAGGAAAGTCCCCTCAAGTGCCTGTAACCTGTGTAGATG TCTTTCGTCCCAGCAACCCTTGGGAACTGGCCAAGACTGTTTTTGGAGCCAAGGAACTGGGCAAGATGGT GGTGGACTGTTGCACAGATCCAGATGGGCGGGCTGTGGATCGTGCCCGGGCCTGGTGTGAGATGGTCGGT ATCCAGTACTTCAGGCTGAACCCCCAGCTAGGCTCAGACATCATGCTGGACGAGGTCAGTGATGCAGTGC TGGTCAACGCCCTCTGGGAGACCGAGGTCTACATCTATGAGCACCGGGAGGAGTTCCAGAAGCTTGTCCA GCTGCTGCTATCTCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270796
Insert Size	2259 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270796.1, NP_001257725.1
RefSeq Size	2992 bp
RefSeq ORF	2259 bp
Locus ID	360426
UniProt ID	P97570
Gene Summary	has Ca2+ independent phospholipase A2 activity; may play a role in regulating transmembrane ionic fluxes in pancreatic beta cells [RGD, Feb 2006] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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