Shkbp1 (NM_001271080) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1309281; Sb1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217125 representing NM_001271080 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTCCCGACGACTGCAGTGGAGGGGGTCCGGAGTCGGGGAGCGCCCGGGGAAGTGATTCATCTGA ACGTAGGGGGCAAGCGGTTCAGCACCTCTCGCCAGACGCTAACCTGGATCCCAGACTCCTTCTTTTCCAG TCTTTTGAGCGGACGTATCTCAACCCTGAAAGATGAGACCGGAGCCATCTTCATCGACCGGGACCCCACT GTCTTTGCCCCCATCCTCAATTTCCTGAGGACCAAAGAGTTAGACCCCAGGGGTGTCCATGGTTCCAGCC TCCTCCATGAAGCCCAATTCTATGGGCTCACTCCACTGGTTCGTCGCCTGCAGGTAAGGGAAGAGTTGGA TCGATCTTCCTGCGGAAATGTCCTCTTCAATGGTTATCTACCTCCTCCAGTGTTCCCAGTGAAGCGGAGA AACCGGCACAGCCTGGTGGGGCCCCAGCAAATAGGGGGACGCCCAGCCCCTGTTCGACGGAGCAACACGA TGCCCCCCAACTTGGGTAATGCAGGGCTGCTAGGCCGAATGCTGGATGATAGAGCACCTCCATCCCCATC AGGCCAACCAGAGGAACCAGGGATGGTGCGGCTGGTGTGTGGTCACCACAACTGGATTGCTGTGGCGTAT ACCCACTTTCTCGTCTGTTATAGACTGAAGGAAGCCTCCGGTTGGCAGCTTGCATTCTCCAGTCCTCGCC TAGACTGGCCCATTGAGCGCCTGGCTCTCACAGCTCGGGTGCTCGGTGGTGCTCCAGGCGAGCATGACAA GATGGTGGCTGCAGCCACAGGCAGCGAGATCCTTCTGTGGGCGCTACAGGCACAAGGTGGTGGCTCTGAG ATAGGTGTCTTCCACCTGGGTGTGCCTGTGGAAGCCTTGTTCTTTGTTGGGAACCAGCTCATTGCCACAA GCCACACAGGGCGCATCGGTGTCTGGAACGCCGTGACCAAGCACTGGCAGGTCCAGGAGGTCCAGCCTAT CACGAGTTATGATGCAGCCGGCTCCTTCCTCCTCCTGGGCTGCAGCAATGGCTCCATCTATTATGTAGAT GTGCAGAAATTCCCCCTGCGCATGAAGGACAACGACCTCCTTGTCAGTGAGCTCTACCGAGACCCAGCAG AAGATGGGGTCACCGCCCTCAGCGTCTACCTCACCCCCAAGACCAGTGACAGTGGGAACTGGATTGAGAT TGCCTATGGCACCAGCTCCGGAGTGGTGCGGGTCATTGTCCAGCACCCCGAGACAGTGGGCTCGGGACCC CAGCTCTTCCAGACCTTCAGTGTACACCGCAGCCCTGTCACCAAGATCATGCTGTCAGAGAAGCACCTCA TCTCTGTGTGTGCCGACAACAACCATGTTCGGACGTGGTCTGTGACCCGCTTTCGAGGCATGATCTCCAC CCAGCCTGGTTCCACTCCACTGGCATCCTTCAAGATCCTGGCACTTGAATCAGCCGATGGGCTCGGTGGC TGCAGTGCTGGCAATGACATTGGTCCCTATGGTGAGCGGGATGACCAGCAGGTGTTCATCCAGAAGGTGG TACCAAATGCCAGCCAGCTGTTTGTGCGTCTCTCCTCCACTGGGCAGCGGGTGTGTTCTGTACGCTCAGT GGATGGCTCCGCTACGACTGCCTTCACAGTGTTGGAGTGTGAGGGCTCCAGGCGGTTGGGCTCCAGACCC CGACGCTACCTGCTCACCGGCCAAGCCAATGGCAGCCTGGCCATGTGGGACCTGACCACTGCCATGGACG GCCTGGGCCAAACCCCTGCAGGCGGCCTGACCGAAGAAGAGCTGATGGACCAACTGGAGCAGTGTGAACT GAGCCCATTGACTTCTTCTCGGGCCTCTTTTCCCAGCCCCTCACCCAGAACCTCTCTTACCAGTCTACAC TCAGCCTCCAGCAACACCTCGCTGTGTGGCCACAGAGGGAGCCCCAGCCCACCGCAGGCAGGGGCCCGGA GCCGCGGGGCAGGAAGCTTCGTGGATCGGTTTAAAGAACTGGCAAGGGGTGCACCGGAGCTCCGAGGGCC ACCAACACCAGCCCCTCGACCCTCCACCAGTCTTGGGAATCCCCTGATACTTCCCAAAAACACACTTAAC GAAACTTCCTTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271080
Insert Size	2115 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271080.1, NP_001258009.1
RefSeq Size	2300 bp
RefSeq ORF	2115 bp
Locus ID	292735
UniProt ID	P0C5J9
Gene Summary	Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.[UniProtKB/Swiss-Prot Function]

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