Man1b1 (NM_001109196) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	ERMan1; RGD1563595
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217061 representing NM_001109196 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCCGCCGCCGCCGGCGCCGGCGCCTCACCGGGACTTCATCTCGGTGACGCTGAGCCTGGGAGAGA GCTACGACAACAGCAAGAGCCGGCGGCGGCGCTCATGCTGGAGGAAATGGAAGCAGCTTTCGAGGTTACA GCGGAATGTGATTCTCTTCGTCCTGGGCTTTCTGATTCTCTGTGGATTTCTGTATTCTCTTCAGGTGTCT GACCAGTGGAAAGCTCTGAGTGGCAGCCGTGCAGAAGTAGAGAAGATGAAGCTAGAAGTCCTGCCTGTCT TACCAGCTCCTCAGAAGGAAAGTGCTGAACCAGAAGGGTTTGCAGACATACTCTCTCAGAAGCGGCAAAG GCATTTGCGGCGGGGCCCTCCGCACCTGCAGATCAGACCCCCCAACACCGTCTCCAAAGATGGGATGCAG GATGATGCTAAGGAGAGGGAAGCAGCCCTGGGGAAGGCTCAGCAGGAAGAAAACACCCAGAGAACCGTCA TCAGCTGGCGGGGAGCAGTAATTGAGCCTGAACAGGCCACTGAACCTCCTTCCAAAAGAGCTGAAGCCTC CATCAAGCCTCTGTTTTTGGCGTCCAGGATATGGAAAGAGCCAGCTCCCCCAAATGAGCGCCAGAAGGGA GTGATTGAGGCCTTTCTCCATGCTTGGAAAGGTTACCAAAAGTTCGCTTGGGGCCATGATGAACTGAAGC CTGTGTCCAAAACCTTCAGTGAGTGGTTTGGCCTGGGCCTCACCCTGATCGATGCCTTGGATACCATGTG GATTTTGGGTCTGAAGCAAGAATTTAAGGAAGCCAGGAAGTGGGTGTCGGAGAACTTAGACTTTCAGAAG AATGTCGATGTCAACCTGTTTGAGAGTACTATCCGAATCTTAGGGGGCCTGCTGAGTGCCTACCACCTGT CTGGGGACAGCCTCTTCCTGAGCAAGGCCGAGGATTTTGGAAACCGGTTAATGCCTGCCTTCACCACACC CTCCAAGATTCCATACTCTGATGTGAACATTGGCACTGGGTTTGCCCACTCGCCCCAGTGGACTTCAGAC AGCACGGTGGCAGAAGTGACGAGCATTCAGTTGGAGTTCCGAGAGCTCTCCCGTCTTACCGGGATTAAGA AATTCCAGGAGGCCGTGGAGGAAGTGACAAAGCACATCCACTCCCTGTCTGGGAAGAAAGACGGGCTGGT GCCTATGTTCATCAACACGAACAGCGGCCTCTTCACCCACCCGGGAGTGTTCACCTTGGGTGCCAGGGCT GACAGCTACTACGAATACCTGCTGAAACAGTGGATCCAGGGTGGCAAGAAGGAGACACAGTTGCTGGAAG ACTATGTTAGAGCCATCGAGGGGATCAAAGCTCATCTGCTTCGACAGTCTCAGCCCAGAAAGCTCACCTT CGTGGGAGAGCTTGCCCATGGCCGCTTCAGTGCCAAGATGGACCACTTGGTGTGCTTCCTGCCAGGGACG CTGGCCTTGGGCGTCCACCATGGCCTACCGGCTGACCACATGGATCTGGCTCGGGCACTCATGGAGACCT GCTACCAGATGAACCAACAGATGGAGACAGGTCTGAGCCCTGAGATTGCACATTTCAACATGTACCCTCG AGCAGATCATAAAGACGTGGAGGTCAAGCCGGCTGATAGGCACAACCTGCTGCGGCCGGAGACCGTGGAA AGCCTGTTCTATCTGTACCGCGTCACAAAAGACCGCAAGTACCAAGACTGGGGCTGGGAAATCCTGCAGA GCTTCAATAAGTACACACGGGTTCCCTCGGGTGGCTATTCTTCTATCAACAACGTCCAGAATTCCCACAA GCCTGAGCCCAGGGACAAGATGGAGAGCTTCTTTGTGGGAGAAACACTGAAGTACCTCTACCTGCTGTTT TCTGATGACCTGGAGCTGCTCGGCCTGGACACCTGTGTGTTTAACACAGAAGCTCATCCCCTGCCCATCT GGTCTCCTGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001109196
Insert Size	1974 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001109196.1, NP_001102666.1
RefSeq Size	3694 bp
RefSeq ORF	1974 bp
Locus ID	499751
UniProt ID	B2GUY0
Gene Summary	Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man(5-6)GlcNAc(2) (By similarity).[UniProtKB/Swiss-Prot Function]

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