Mx1 (NM_001271059) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	IFI78
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217052 representing NM_001271059 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAGCAGACCTCTGCATGCCGTCACCAGACACCACAGAAACATCCTGATACCTCTGAAGAGAGCC AGGCAATGGAGTCTGTGGATAACCTCTGCAGTCAGTATGAGGAGAAGGTGCGGCCCTGCATTGACCTCAT CGACTCCCTGCGAGCTCTGGGTGTGGAGCAAGACCTGGCCCTGCCTGCCATCGCTGTCATCGGGGACCAG AGTTCAGGGAAGAGCTCTGTGCTGGAAGCACTGTCTGGAGTGGCCCTCCCCAGAGGCAGTGGTATTGTTA CCAGGTGTCCTCTGGTGCTGAAATTGAAGCAACTGAAGCAGGGAGAAAAATGGAGCGGCAAGGTCATTTA TAAGGACACCGAGATTGAGATCTCACACCCTTCACTGGTAGAAAGGGAAATCAATAAAGCCCAGAACTTG ATTGCTGGGGAAGGGTTGAAGATTAGCTCTGATCTCATTAGCTTGGAGGTTAGCTCTCCACATGTCCCAG ACCTGACTCTGATTGACCTTCCTGGTATCACAAGAGTGGCTGTGGGTGACCAGCCTGCAGACATCGAACA CAAGATCAAGAGACTTATCACTGAATACATCCAGAAACAGGAGACCATCAACCTGGTGGTGGTCCCCAGC AATGTGGACATTGCCACCACAGAGGCCCTGAAAATGGCTCAGGAGGTGGACCCTCAAGGGGATAGAACCA TAGGGATCTTGACCAAGCCCGATCTGGTGGACAGAGGGACTGAAGACAAGGTCGTGGATGTGGTGCGGAA CCTCGTGTGCCACCTGAAGAAGGGCTACATGATAGTCAAGTGCAGGGGTCAGCAGGACATCCAGGAGCAG CTGAGCCTGGCTGAGGCTCTTCAGAAGGAGCAAGTCTTCTTCAAGGAACACCCTCAATTCAGAGTTCTTC TCGAGGATGGGAAGGCCACAGTGCCCTGCTTGGCAAAGAGACTGACCATGGAGCTCACCTCCCACATCTG TAAATCGCTGCCAATATTGGAAAATCAAATAAATGTGAATCATCAGATTGCGAGTGAGGAGCTGCAGAAG TACGGAGCAGACATACCAGAAGATGACAGTAAGAGGCTGTCCTTTCTGATGAATAAAATCAATGTCTTCA ATAAGGATATCCTAAGTTTGGTACAAGCACAAGAAAACATATCATGGGAAGAAAGCCGGCTGTTCACCAA ACTGCGAAATGAGTTTCTTGCTTGGAATGATTATATTGAGGAACATTTCAAAAAAACCCTAGGTTCTTCT GAGAAACACAGCCAGATGGAAAAATTTGAAAGTCATTATCGTGGCCGGGAACTGCCAGGGTTTGTGGACT ATAAGGCATTTGAGAACATTATCAAGAAGGAAGTCAAGGCCCTGGAAGAACCCGCTCTGAACATGCTGCA CAGGGTCACTACCATGGTCAAAAATGCCTTCACAAAGGTTTCATCAAACAATTTTGGTGATTTTTTAAAC CTCCACAGTACTGCCAAGTCCAAAATTGAAGACATCAGATTTAGCCAAGAAAAAGAAGCCGAAAAACTGA TCCGACTTCACTTCCAGATGGAACACATTGTCTACTGCCAGGACCAGGCTTACAAGAAAGCCTTGAAGGA GATCAGAGAGAAGGAAGCTGAGAAAGAGAAGTCAACCTTTGGTGTCTTTCAACAAAACTCTCCTCGAAAG GAGTTGACTACCACTGAGATGACCCAGCACCTGAATGCCTACTACCAGGAGTGCGGAAGGAACATTGGGA GACAGATCCCTCTGATCATCCAGTACTCCATCCTGCAAACATTTGGGCAGGAAATGGAGAAAGCAATGCT TCAGCTTCTACAGGACACCAGTAAGTGCAACTGGTTCCTGACTGAGCAGAGTGACAGCAGAGAGAAGAAG AAGTTCCTGAAGAGGCGGCTTTTAAGGCTGGATGAGGCTCAGCGGAAGCTTGCCAAATTCTCCAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271059
Insert Size	1959 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271059.1, NP_001257988.1
RefSeq Size	3247 bp
RefSeq ORF	1959 bp
Locus ID	24575
Gene Summary	mouse homolog is an interferon inducible gene that confers influenza resistance to mice [RGD, Feb 2006] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All six variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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