Gls2 (NM_001270786) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ga
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216984 representing NM_001270786 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTCCATGAGGGCTCTGCAGAACGCGCTGAGCCGCGCGGGCAGCCACGGCCAGAGGGGAGGCTGGG GTCACCCGAGCCGGGGCCCGCTCCTGGGCGGGGGTGTCCGGTACTACTTCGGTGAGGCCGCGGCGCAGGG CAGGGGGACGCCGCACAGCCACCAGCCGCAGCACTCGGACCATGATGCCTCGAACAGTGGCATGTTGCCT CGACTTGGTGACCTGCTTTTCTACACCATTGCAGAGGGGCAGGAACGCATCCCCATCCATAAGTTCACCA CGGCCCTGAAGGCCACTGGACTGCAGACGTCAGACCCACGGCTCCAGGACTGCATGAGCAAGATGCAGCG CATGGTCCAAGAGTCCAGCAGTGGTGGCCTCTTGGACCGAGAACTCTTCCAAAAGTGTGTGAGCAGCAAC ATTGTGCTCCTGACTCAGGCATTCCGGAAGAAGTTTGTCATTCCTGACTTTGAGGAGTTCACAGGCCATG TGGATCGTATATTTGAGGATGCCAAAGAGCTCACTGGAGGCAAAGTGGCAGCCTACATCCCTCACCTGGC CAAGTCAAACCCAGATCTTTGGGGCGTCTCCCTGTGCACTGTGGATGGGCAGCGACACTCTGTGGGCCAC ACGAAGATCCCTTTCTGCCTACAGTCCTGTGTCAAGCCACTCACTTACGCCATCTCCGTGAGCACCTTAG GCACCGACTACGTGCACAAGTTCGTGGGCAAGGAACCCAGTGGTCTGCGCTATAACAAACTCTCCCTCAA CGAGGAAGGAATCCCTCATAACCCCATGGTCAATGCTGGTGCTATTGTGGTCAGCTCCCTGATCAAGATG GACTGTAACAAAGCAGAGAAGTTTGACTTTGTGTTACAGTATCTGAACAAGATGGCTGGGAATGAATTCA TGGGGTTCAGCAATGCCACATTCCAGTCAGAGAAGGAGACTGGGGATCGGAATTACGCCATTGGCTATTA CCTCAAGGAGAAGAAGTGCTTCCCTAAGGGAGTGGACATGATGGCTGCCCTTGATCTCTATTTCCAGCTG TGCTCTGTGGAGGTTACCTGTGAGTCAGGCAGTGTCATGGCGGCCACTCTTGCCAATGGCGGCATCTGCC CCATCACAGGGGAGAGCGTGCTGAGTGCGGAAGCCGTGCGCAACACCCTCAGCCTCATGCACTCCTGTGG CATGTATGACTTCTCGGGCCAGTTTGCCTTCCACGTGGGCCTGCCAGCCAAGTCAGCTGTGTCGGGAGCC ATCCTCCTGGTTGTACCCAATGTCATGGGGATGATGTGTCTGTCACCCCCGTTAGACAAGCTGGGGAACA GCCACAGGGGCATCAGCTTCTGCCAGAAGTTGGTGTCTCTGTTTAACTTCCACAACTACGACAACCTGCG GCACTGTGCTCGGAAGTTAGACCCACGGAGGGAAGGGGGGGAAGTTCGGAACAAGACCGTGGTGAACCTG TTATTTGCTGCATATAGTGGAGATGTCTCAGCTCTTCGAAGGTTTGCCTTGTCTGCCGTGGATATGGAGC AGAAGGACTATGATTCCCGCACAGCCCTACATGTGGCGGCAGCGGAAGGACACATTGACGTTGTCAAGTT TCTGATCGAGGCTTGCAAAGTGAATCCTTTTGTCAAGGACAGGTGGGGCAACATTCCCCTGGATGATGCC GTGCAGTTCAATCACCTGGAGGTGGTCAAACTGCTTCAGGATTACCATGACTCCTACATGCTGTCTGAGA CTCAAGCTGAGGTAGCAGCTGAGACTCTGTCAAAAGAGAACTTAGAGAGCATGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270786
Insert Size	1809 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270786.1, NP_001257715.1
RefSeq Size	2552 bp
RefSeq ORF	1809 bp
Locus ID	192268
UniProt ID	P28492
Gene Summary	first enzyme of glutamine catabolism in liver [RGD, Feb 2006] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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