Ptpn11 (NM_001177593) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Shp2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216979 representing NM_001177593 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATCCCGGAGATGGTTTCACCCCAATATCACTGGTGTGGAGGCAGAGAATCTCCTGCTGACCCGAG GAGTCGATGGCAGTTTCTTAGCGAGGCCCAGCAAGAGTAACCCTGGAGACTTCACTCTGTCTGTTAGAAG AAATGGAGCCGTTACCCACATCAAGATTCAGAACACTGGGGACTACTATGACCTCTATGGCGGGGAAAAG TTTGCCACCTTGGCTGAACTGGTCCAGTATTACATGGAGCATCACGGGCAGCTGAAAGAGAAGAATGGAG ATGTTATTGAGCTCAAGTACCCACTGAACTGTGCAGACCCTACCTCTGAAAGGTGGTTCCACGGTCACTT GTCTGGAAAAGAAGCAGAGAAGCTGCTGACAGAGAAGGGGAAGCATGGCAGTTTCCTCGTCCGGGAGAGC CAGAGCCACCCTGGGGACTTCGTCCTCTCCGTCCGCACTGGTGATGACAAAGGGGAGAGCAATGACAGCA AGTCCAAAGTGACCCATGTCATGATCCGCTGTCAGGAGCTGAAATATGATGTTGGTGGAGGAGAGCGCTT TGACTCTTTGACAGACCTGGTGGAGCATTACAAGAAGAACCCCATGGTGGAGACACTGGGCACAGTCCTG CAGCTCAAACAGCCCCTCAACACAACTCGTATTAATGCCGCTGAAATCGAAAGCCGGGTTCGGGAGTTAA GCAAGCTAGCCGAGACCACAGATAAAGTCAAACAGGGCTTTTGGGAAGAATTTGAGACTCTACAGCAACA GGAATGCAAACTTCTCTACAGCCGAAAAGAAGGACAGAGACAAGAAAATAAAAACAAAAATAGATACAAA AACATCCTGCCCTTTGATCATACCAGGGTTGTCCTGCACGATGGGGATCCCAACGAGCCAGTTTCTGATT ACATCAATGCCAACATCATCATGCCTGAATTTGAAACCAAGTGCAACAATTCAAAACCCAAAAAGAGTTA CATTGCCACTCAAGGCTGCCTGCAGAACACGGTGAATGACTTCTGGCGGATGGTGTTCCAGGAGAACTCT CGAGTCATTGTCATGACCACAAAGGAAGTGGAGAGAGGGAAGAGCAAGTGTGTCAAGTACTGGCCTGATG AGTGTGCACTCAAAGAGTATGGCGTCATGCGTGTGAGGAACGTCAGAGAAAGTGCTGCGCATGACTACAC CTTACGAGAACTCAAACTCTCTAAGGTCGGACAAGCTCTACTCCAGGGAAACACAGAGAGAACCGTCTGG CAGTACCACTTTCGGACCTGGCCAGACCACGGTGTGCCTAGTGACCCTGGAGGTGTGCTGGACTTCCTGG AGGAGGTCCACCACAAGCAGGAGAGCATCGTGGATGCAGGCCCTGTCGTGGTTCACTGCAGTGCTGGGAT TGGCCGGACAGGAACGTTCATTGTGATTGATATCCTTATTGACATCATCCGAGAGAAAGGTGTGGACTGT GACATCGACGTTCCTAAAACCATTCAGATGGTACGGTCCCAGAGGTCAGGGATGGTCCAGACAGAAGCAC AGTACCGGTTCATCTACATGGCCGTCCAGCACTACATAGAGACGCTGCAACGCAGGATCGAGGAGGAGCA GAAAAGCAAAAGGAAAGGACATGAATATACCAATATTAAGTATTCCCTGGTGGACCAGACAAGTGGCGAT CAGAGTCCCCTGCCACCTTGCACCCCAACGCCACCCTGTGCGGAAATGCGGGAGGACAGCGCTCGAGTCT ATGAGAACGTGGGCCTCATGCAGCAGCAGAGGAGTTTCAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001177593
Insert Size	1794 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001177593.1, NP_001171064.1
RefSeq Size	5538 bp
RefSeq ORF	1794 bp
Locus ID	25622
UniProt ID	P41499
Gene Summary	catalyzes the tyrosine dephosphorylation of para-nitrophenylphosphate, nicotinic acetylcholine receptor, myelin basic protein, and other proteins [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.