Ptbp1 (NM_001271057) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ptb; PTB1; Pybp; Pybp1; Pybp2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216906 representing NM_001271057 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGCATCGTCCCAGACATAGCAGTCGGTACAAAGCGGGGATCCGACGAGCTCTTCTCCACGTGTG TCAGCAACGGCCCCTTCATCATGAGCAGCTCGGCCTCAGCAGCCAACGGAAATGATAGCAAGAAGTTCAA AGGTGATAACAGGAGCACAGGAGTTCCTTCCAGAGTCATCCATGTCCGAAAGCTACCTAGTGATGTCACT GAGGGCGAGGTCATCTCCCTGGGGCTGCCCTTTGGGAAAGTTACCAACCTCCTTATGCTGAAGGGGAAGA ACCAGGCCTTCATTGAAATGAGCACAGAGGAGGCTGCCAACACTATGGTTAACTACTACACATCGGTGGC GCCAGTGCTTCGTGGACAGCCCATCTACATCCAGTTCTCCAACCACAAGGAGCTCAAAACCGACAGCTCG CCCAACCAGGCACGTGCCCAGGCAGCCCTGCAGGCTGTGAACTCCGTACAGTCAGGAAACCTGGCCTTGG CAGCCTCTGCTGCTGCCGTGGATGCAGGAATGGCAATGGCAGGCCAGAGCCCAGTGCTCAGGATCATTGT GGAGAACCTTTTCTACCCAGTGACACTGGACGTGCTGCACCAGATCTTCTCGAAGTTTGGCACAGTCCTG AAGATCATCACGTTCACCAAGAACAACCAGTTCCAGGCGCTGCTGCAGTATGCTGACCCTGTGAGCGCCC AGCATGCCAAGCTGTCCCTGGATGGCCAGAACATCTACAATGCCTGCTGCACGCTGCGCATCGACTTCTC CAAGCTCACCAGTCTCAACGTCAAATACAACAACGACAAGAGCCGAGACTACACACGCCCTGACCTGCCC TCTGGAGACAGCCAGCCATCACTGGATCAGACCATGGCAGCAGCCTTTGGTGCGCCCGGCATAATGTCAG CCTCTCCGTATGCAGGAGCCGGGTTCCCTCCCACCTTTGCCATCCCTCAGGCCGCAGGCCTCTCTGTTCC TAATGTCCATGGAGCCCTGGCACCCCTGGCCATACCCTCTGCTGCTGCTGCTGCTGCTGCAGCCGGCCGC ATTGCCATCCCAGGGCTGGCAGGCGCCGGGAATTCCGTCCTTTTGGTCAGCAACTTGAACCCTGAGAGAG TCACACCCCAAAGCCTCTTTATTCTCTTCGGCGTCTACGGTGATGTGCAGCGGGTGAAGATCCTGTTCAA CAAGAAGGAGAACGCACTTGTGCAGATGGCAGACGGCAGCCAGGCCCAGCTGGCCATGAGCCACCTGAAC GGGCACAAGCTGCATGGGAAGTCAGTGCGCATCACCCTGTCAAAGCACCAGAGTGTGCAGCTGCCTCGTG AGGGACAGGAGGACCAGGGCCTGACCAAGGACTATGGCAGCTCACCGCTGCACCGCTTCAAGAAACCTGG CTCCAAGAACTTTCAGAACATCTTTCCTCCCTCAGCTACCCTGCACCTCTCCAACATCCCGCCCTCTGTG TCAGAGGATGACCTCAAGAGCCTCTTCTCCAGCAATGGCGGTGTGGTCAAAGGCTTCAAGTTCTTCCAGA AGGACCGCAAGATGGCACTGATCCAGATGGGCTCTGTGGAGGAGGCAGTGCAGGCACTAATTGAGCTGCA CAACCATGACCTGGGCGAGAACCACCACCTGCGAGTGTCCTTTTCCAAGTCCACCATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271057
Insert Size	1671 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271057.1, NP_001257986.1
RefSeq Size	2905 bp
RefSeq ORF	1671 bp
Locus ID	29497
Gene Summary	DNA binding protein; involved in the negative regulation of alternative splicing [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform a).

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