Slc1a3 (NM_001289941) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	EAAT1; GLAST; GLAST-1; GluT-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216790 representing NM_001289941 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAAAAAGCAACGGAGAAGAGCCCAGGATGGGAAGCAGGATGGAAAGATTCCAGCAAGGGGTGCGCA AGCGGACGCTCCTGGCCAAGAAGAAAGTTCAGAACATCACCAAGGAGGATGTGAAGAGCTACCTGTTTCG GAATGCCTTTGTGCTACTCACCGTCAGCGCTGTCATTGTGGGAATGGCGGCCCTAGATAGTAAGGCATCT GGGAAGATGGGGATGCGAGCTGTGGTCTATTACATGACTACCACCATCATTGCTGTGGTGATCGGCATAA TCATTGTCATCATCATCCACCCCGGAAAGGGCACGAAAGAAAACATGTACAGAGAAGGTAAAATCGTGCA GGTGACTGCCGCAGATGCCTTCCTGGATTTAATCAGGAACATGTTCCCACCCAATCTGGTAGAAGCCTGC TTTAAACAGTTTAAAACCAGCTATGAGAAGAGAAGCTTTAAAGTGCCCATCCAGGCCAACGAAACACTGT TGGGCGCCGTGATCAACAACGTGTCAGAGGCCATGGAGACTCTGACCCGGATCCGGGAGGAGATGGTACC CGTTCCTGGGTCTGTGAATGGGGTCAATGCCCTGGGCCTCGTTGTCTTCTCCATGTGCTTCGGCTTCGTG ATCGGAAACATGAAGGAGCAGGGGCAAGCGCTAAGAGAGTTCTTCGACTCTCTCAATGAAGCCATCATGA GATTGGTAGCGGTGATAATGTGGTATGCACCTCTGGGCATCCTCTTCTTGATCGCAGGGAAGATTGTTGA GATGGAAGATATGGGTGTGATTGGGGGGCAGCTTGCCATGTATACAGTGACAGTCATCGTCGGCCTCCTC ATTCATGCCGTCATCGTCCTGCCTCTCCTCTACTTCCTGGTAACCCGGAAGAACCCCTGGGTTTTCATTG GAGGGTTGCTGCAAGCACTCATCACAGCCCTGGGGACCTCCTCAAGTTCTGCCACCCTGCCCATCACTTT CAAGTGCCTGGAAGAAAACAATGGTGTGGACAAACGCATCACCAGATTTGTGCTTCCCGTGGGGGCCACC ATTAACATGGATGGGACCGCCCTCTACGAGGCTTTGGCCGCCATTTTCATCGCTCAAGTTAACAACTTTG ACCTGAATTTTGGACAGATTATAACAATAAGTATCACAGCCACAGCCGCAAGCATTGGGGCAGCTGGCAT TCCTCAGGCCGGTCTAGTCACCATGGTCATCGTGCTGACATCTGTGGGCCTGCCCACGGATGACATCACA CTCATCATTGCAGTGGACTGGTTTCTGGACCGCCTCCGAACCACCACCAACGTGCTGGGTGACTCCCTCG GGGCCGGGATTGTTGAACACTTGTCCCGACATGAACTGAAGAACCGAGATGTTGAAATGGGGAACTCCGT GATTGAGGAGAATGAAATGAAAAAGCCGTATCAGCTGATTGCCCAGGACAATGAACCAGAGAAACCCGTG GCAGACAGCGAAACCAAGATGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289941
Insert Size	1494 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289941.1, NP_001276870.1
RefSeq Size	3805 bp
RefSeq ORF	1494 bp
Locus ID	29483
UniProt ID	P24942
Gene Summary	carries out Na+ dependent transport of glutamate and aspartate; may regulate neurotransmitter concentration in the central nervous system [RGD, Feb 2006] Transcript Variant: This variant (Glast1a, PMID:11086157) lacks an alternate in-frame exon in the 5' coding region, compared to variant Glast. The encoded protein (isoform 2) is shorter, compared to isoform 1.

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