Ntrk2 (NM_001163168) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RATTRKB1; Tkrb; trk-B; trkB; TRKB1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216757 representing NM_001163168 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCCCTGGCCGAGGTGGCATGGACCCGCCATGGCGCGGCTCTGGGGCTTATGCTTGCTGGTCTTGG GCTTCTGGAGGGCTTCTCTTGCCTGCCCCATGTCCTGCAAATGCAGCACCACTAGGATTTGGTGTACCGA GCCTTCTCCTGGCATCGTGGCATTTCCGAGGTTGGAACCTAACAGCATTGACCCAGAGAACATCACCGAA ATTCTCATTGCAAACCAGAAAAGGTTAGAAATCATCAATGAAGATGATGTCGAAGCTTACGTGGGGCTGA AAAACCTTACAATTGTGGATTCCGGCTTAAAGTTTGTGGCTTACAAGGCGTTTCTGAAGAACGGCAACCT GCGGCACATCAATTTCACTCGAAACAAGCTGACGAGTTTGTCCAGGAGACATTTCCGCCACCTTGACTTG TCTGACCTGATCCTGACGGGTAATCCGTTCACGTGTTCCTGTGACATCATGTGGCTCAAGACTCTCCAGG AGACGAAATCCAGCCCCGACACTCAGGATTTGTATTGCCTCAATGAGAGCAGCAAGAATACCCCTCTGGC GAACCTGCAGATTCCCAATTGTGGTCTGCCGTCTGCACGTCTGGCCGCTCCTAACCTCACGGTGGAGGAA GGGAAGTCTGTGACCATTTCCTGCAGCGTCGGGGGTGACCCGCTCCCCACCTTGTACTGGGACGTTGGGA ATTTGGTTTCCAAACACATGAATGAAACAAGCCACACACAGGGCTCCTTAAGGATAACAAACATTTCATC GGATGACAGTGGGAAACAAATCTCTTGTGTGGCAGAAAACCTCGTTGGAGAAGATCAAGACTCTGTGAAC CTCACTGTGCATTTTGCACCAACCATCACATTTCTCGAATCTCCAACCTCAGACCACCACTGGTGCATCC CATTCACTGTGAGAGGCAACCCCAAGCCAGCACTTCAGTGGTTCTACAACGGAGCCATACTGAATGAATC CAAGTACATCTGTACCAAAATACACGTCACCAATCACACGGAGTACCACGGCTGCCTCCAGCTGGATAAC CCCACTCATATGAATAATGGAGACTACACCCTAATGGCCAAGAATGAATATGGGAAGGACGAGAGACAGA TTTCTGCTCACTTCATGGGCCGGCCTGGAGTTGACTATGAGACAAACCCAAATTACCCTGAAGTCCTCTA TGAAGACTGGACCACGCCAACTGACATCGGGGATACTACAAACAAAAGTAATGAGATCCCCTCCACGGAT GTTGCTGACCAAACCAATCGGGAGCATCTCTCGGTCTATGCCGTGGTGGTGATTGCCTCTGTGGTAGGAT TCTGCCTGCTGGTGATGCTGCTTCTGCTCAAGTTGGCGAGACATTCCAAGTTTGGCATGAAAGGTTTTGT TTTGTTTCATAAGATCCCCCTGGATGGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001163168
Insert Size	1431 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001163168.2, NP_001156640.1
RefSeq Size	6931 bp
RefSeq ORF	1431 bp
Locus ID	25054
UniProt ID	Q63604
Gene Summary	receptor for brain-derived neurotrophic factor (BDNF) [RGD, Feb 2006] Transcript Variant: This variant (2) lacks multiple coding exons and uses a distinct terminal exon compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus compared to to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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