Ulk3 (NM_001271135) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216748 representing NM_001271135 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGTCCGGCTGGGGTCTCCCTCGGCTGGACGGTTTCATCCTTACCGAGCGCCTGGGCAGTGGCA CGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGCTACTCGGGAAGTGGTAGCCATAAAATGCGTGGC CAAGAAAAGTCTCAACAAGGCGTCAGTGGAAAACCTCCTGACTGAGATTGAGATCCTCAAGGGCATTCGG CACCCCCATATCGTGCAGCTGAAAGACTTCCAGTGGGACAATGACAATATCTACCTCATCATGGAGTTCT GTGCAGGGGGTGACCTGTCTCGCTTCATCCATACCCGCAGGATTCTGCCTGAGAAGGTGGCCCGTGTTTT CATGCAGCAGTTGGCTAGTGCCCTGCAGTTCCTGCATGAACGAAACATCTCTCACTTGGATCTGAAGCCG CAGAACATCCTCCTGAGTTCTTTGGAGAAGCCCCACCTGAAACTGGCAGACTTTGGCTTTGCCCAGCATA TGTCCCCGTGGGATGAGAAACATGTGCTTCGTGGCTCCCCGCTCTACATGGCTCCTGAGATGGTGTGTCG GCGGCAGTATGACGCGCGTGTGGACCTCTGGTCTGTGGGGGTCATCCTGTACGAAGCTCTCTTTGGGCAG CCTCCCTTTGCCTCCAGATCGTTCTCAGAGCTAGAAGAGAAGATTCGTAGCAATCGGGTTATTGAGCTCC CTCTTCGGCCCCAACTCTCCCTAGACTGCCGGGACCTGTTGCAGCGACTTCTAGAGCGGGACCCCAGCCA TCGAATCTCCTTTCAGGACTTCTTTGCCCACCCCTGGGTGGACCTGGAGCACATGCCTAGTGGGGAGAGC CTGGCACAAGCAACAGCCCTTGTGGTGGAGGCCGTGAAGAAGGACCAGGAGGGGGACGCTGCTGCTGCCC TGTCACTCTACTGCAAGGCCCTGGACTTCTTTGTACCTGCACTACACTACGAAGTGGATGCCCAGAGGAA AGAGGCGATTAAGGCGAAGGTGGGGCAGTATGTGTCCAGGGCAGAGGAGCTCAAAGCCATTGTCTCCTCC TCCAATCAGGCCCTGCTAAGGCAGGGCACAACTGGCCAAGAGCTGCTGCGAGAGATGGCCCGTGACAAGC CACGCCTCCTAGCTGCCCTGGAAGTGGCCTCAGCTGCCATGGCCAAGGAGGAGGAAGCTGGCAAAGAGCA GGATGCCCTGGACCTGTACCAGCACAGCCTCGGGGAGCTGCTACTGCTGTTGGCAGCAGAAGCCCCAGGC CGAAGGCGGGAGCTCCTTCACACTGAGGTTCAGAACCTCATGGCTCGAGCTGAATACCTGAAGGAGCAGA TCAAGATCAGGGAATCTCACTGGGAAGCAGAGAGTCTGGACAAAGAGGGGCTGTCGGAGTCTGTTCGAAG TTCTTGCACACTGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271135
Insert Size	1419 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271135.1, NP_001258064.1
RefSeq Size	1729 bp
RefSeq ORF	1419 bp
Locus ID	691171
UniProt ID	D3ZHP7
Gene Summary	Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy (By similarity).[UniProtKB/Swiss-Prot Function]

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