Mapk8 (NM_053829) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	JNK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216642 representing NM_053829 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGAAGTAAACGTGACAACAATTTTTATAGTGTAGAGATCGGAGATTCTACATTCACAGTCCTAA AACGATACCAGAACTTAAAGCCTATAGGCTCAGGAGCTCAAGGAATAGTGTGTGCAGCTTATGATGCTAT TCTTGAAAGAAATGTTGCAATCAAGAAGCTCAGCCGGCCATTTCAGAATCAGACCCATGCTAAGCGAGCC TACCGAGAACTAGTTCTTATGAAGTGTGTTAATCACAAAAATATAATTGGCCTTTTGAATGTTTTCACAC CACAGAAATCCCTAGAAGAATTTCAAGATGTTTACATAGTCATGGAGCTCATGGATGCAAATCTTTGCCA AGTGATTCAGATGGAGTTAGATCATGAAAGAATGTCCTACCTTCTCTATCAAATGCTGTGTGGAATCAAG CACCTTCACTCTGCTGGAATTATTCATCGGGACTTAAAGCCTAGTAATATAGTAGTCAAATCAGACTGCA CTTTGAAGATTCTTGATTTTGGACTGGCAAGGACTGCAGGAACGAGTTTTATGATGACGCCTTACGTGGT AACTCGTTACTACAGAGCACCAGAGGTCATTCTCGGCATGGGCTACAAGGAGAACGTGGATTTATGGTCT GTGGGGTGCATTATGGGAGAAATGGTTTGCCTCAAAATCCTCTTTCCAGGAAGGGACTATATTGATCAGT GGAATAAAGTTATTGAACAGCTCGGAACACCTTGTCCTGAATTCATGAAGAAACTACAACCAACAGTAAG GACTTACGTTGAAAACAGACCTAAGTACGCTGGCTATAGCTTTGAGAAACTGTTTCCTGATGTGCTTTTC CCAGCTGACTCAGAACATAACAAACTTAAAGCCAGTCAGGCGAGAGATTTGTTATCTAAAATGCTGGTGA TAGATGCGTCCAAAAGGATCTCCGTAGACGAAGCTCTCCAGCACCCGTACATCAACGTCTGGTATGATCC TTCAGAAGCAGAGGCCCCACCACCAAAGATCCCTGACAAGCAGTTAGATGAAAGGGAGCACACAATAGAG GAGTGGAAAGAACTGATATACAAGGAGGTCATGGATTTGGAGGAGCGAACTAAGAATGGCGTCATAAGAG GGCAGCCGTCTCCTTTAGGTGCAGCAGTGATCAATGGCTCTCAGCATCCGGTCTCTTCGCCGTCTGTCAA TGACATGTCTTCAATGTCCACAGATCCGACTCTGGCCTCGGATACAGACAGCAGTCTAGAAGCATCAGCT GGACCTCTGGGCTGCTGTAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053829
Insert Size	1284 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053829.2, NP_446281.2
RefSeq Size	5796 bp
RefSeq ORF	1284 bp
Locus ID	116554
UniProt ID	P49185
Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. [provided by RefSeq, Jul 2012]

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