P2rx3 (NM_001270621) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216512 representing NM_001270621 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTGTATATCAGACTTCTTCACCTACGAGACTACCAAGTCGGTGGTTGTGAAGAGCTGGACCATTG GGATCATCAACCGAGCCGTCCAGCTGCTGATTATCTCCTACTTTGTGGGGTGGGTTTTCTTGCATGAGAA GGCCTACCAAGTGAGGGACACCGCCATTGAGTCCTCAGTAGTTACAAAGGTGAAAGGCTTCGGGCGCTAT GCCAACAGAGTCATGGACGTGTCGGATTATGTGACCCCACCCCAGAATGAAGAGAAGTACCGCTGTGTGT CTGACAGCCAGTGTGGGCCTGAACGCTTCCCAGGTGGGGGGATCCTCACCGGCCGCTGCGTGAACTACAG CTCTGTTCTCCGGACCTGTGAGATCCAGGGCTGGTGCCCCACTGAGGTGGACACCGTGGAGATGCCTATC ATGATGGAGGCTGAGAACTTCACCATTTTCATCAAGAACAGCATCCGTTTCCCTCTCTTCAACTTTGAGA AGGGAAACCTCCTGCCTAACCTCACCGACAAGGACATAAAGAGGTGCCGCTTCCACCCTGAAAAGGCCCC ATTTTGCCCCATCTTGAGGGTAGGGGATGTGGTTAAGTTTGCTGGACAGGATTTTGCCAAGCTGGCCCGC ACGGGTGGCGTTCTGGGTATTAAGATCGGCTGGGTGTGCGATCTAGACAAGGCCTGGGACCAGTGCATCC CTAAATATTCCTTCACTCGGCTGGATGGAGTTTCTGAGAAAAGCAGTGTTTCCCCTGGCTACAACTTCAG GTTTGCCAAATACTATAAGATGGAGAACGGCAGCGAGTACCGCACACTCCTGAAGGCTTTTGGCATCCGC TTTGATGTGCTGGTATATGGGAACGCTGGCAAGTTCAACATCATCCCCACCATTATCAGCTCGGTGGCGG CCTTCACTTCTGTGGGAGTGGGCACTGTTCTCTGTGACATCATCCTGCTCAATTTCCTCAAAGGGGCTGA TCACTACAAAGCCAGGAAGTTTGAGGAGGTGACTGAGACAACACTGAAGGGTACTGCGTCAACCAACCCA GTGTTCGCCAGTGACCAGGCCACTGTGGAGAAGCAGTCTACAGACTCAGGGGCCTATTCTATTGGTCACT AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270621
Insert Size	1122 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270621.1, NP_001257550.1
RefSeq Size	3692 bp
RefSeq ORF	1122 bp
Locus ID	81739
UniProt ID	P49654
Gene Summary	ligand gated, cation-selective ion channel that is a receptor for ATP [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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