Ctdnep1 (NM_001100494) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Dullard
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216124 representing NM_001100494 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCGGACGCAGTGTCTGCTGGGGCTGCGCACGTTCGTGGCCTTCGCCGCCAAGCTCTGGAGCTTCT TTATTTACCTTTTGCGGAGGCAGATCCGCACGGTAATTCAGTATCAAACTGTTCGATATGATATCCTTCC CTTATCTCCTTTGTCCCGGAATCGACTAGCCCAGGTGAAGAGGAAGATCCTGGTGCTGGATCTGGATGAA ACCCTTATTCATTCTCACCACGATGGGGTCCTGAGGCCTACAGTGAGGCCTGGAACACCTCCTGATTTCA TCCTGAAGGTGGTAATAGACAAACATCCTGTCCGGTTTTTTGTACATAAGAGACCCCATGTGGATTTCTT CTTAGAAGTGGTAAGCCAGTGGTATGAGCTTGTGGTGTTTACAGCAAGCATGGAAATTTATGGCTCTGCT GTGGCAGATAAACTGGACAATAGCAGAAGCATTCTTAAGAGGAGATACTACAGACAGCACTGCACTTTGG AGTTGGGCAGCTACATCAAAGACCTCTCTGTGGTCCACAGTGACCTTTCCAGCATCGTGATCCTGGACAA CTCCCCTGGGGCTTACAGGAGCCACCCAGACAATGCCATCCCCATCAAATCCTGGTTCAGTGACCCCAGT GACACAGCCCTTCTCAACCTTCTCCCAATGTTGGACGCCCTCAGGTTCACTGCTGATGTCCGATCGGTGC TGAGCCGAAACCTTCACCAACATAGGCTCTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100494
Insert Size	735 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100494.1, NP_001093964.1
RefSeq Size	1530 bp
RefSeq ORF	735 bp
Locus ID	287447
UniProt ID	Q3B7T6
Gene Summary	Serine/threonine protein phosphatase forming with CNEP1R1 an active phosphatase complex that dephosphorylates and may activate LPIN1 and LPIN2. LPIN1 and LPIN2 are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at different levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol. May antagonize BMP signaling (By similarity).[UniProtKB/Swiss-Prot Function]

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