Gsta3 (NM_001287025) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Gsta5; Yc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215946 representing NM_001287025 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGAACAAGTGCCCATGGTGGAGATTGACGGGATGAAGCTGGTGCAGACCAAAGCCATTCTCAACT ACATTGCCACCAAATACAACCTCTATGGGAAGGACATGAAGGAGAGAGCCCTCATCGACATGTATGCAGA AGGTGTGGCCGATCTGGAGTTGATGGTTCTCTATTACCCCTACATGCCCCCTGGGGAGAAAGAGGCGAGT CTTGCCAAGATCAAGGACAAAGCAAGGAACCGTTACTTCCCTGCCTATGAGAAGGTGTTGAAGAGCCACG GACAAGATTATCTCGTTGGCAACAAGCTGAGCAGGGCTGATGTTTCCCTGGTTGAACTTCTCTACCATGT GGAAGAGATGGACCCAGGCATTGTGGACAACTTCCCTCTGCTAAAGGCCCTGAGAACCAGAGTCAGCAAC CTCCCCACAGTGAAGAAATTTCTTCAGCCTGGCAGCCAGAGGAAGCCTTTTGATGATGAGAAATGTGTAG AATCAGCGAAGAAGATCTTCAGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001287025
Insert Size	516 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287025.1, NP_001273954.1
RefSeq Size	1370 bp
RefSeq ORF	516 bp
Locus ID	494500
Gene Summary	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate 5' exon compared to variant 1 that results in the use of a downstream translation initiation codon. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the Celera genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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