Cit (NM_001029911) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215463 representing NM_001029911 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGAAGTTCAAGTATGGAGTGCGGAACCCGTCCGAGGCCAGTGCCCCCGAGCCCATTGCCAGTCGGG CCTCCAGGCTAAATCTCTTCTTCCAGGGGAAACCGCCCCTCATGACTCAACAGCAGATGTCTGCTCTTTC CCGGGAAGGGGTGTTAGATGCCCTCTTTGTTCTCTTGGAAGAGTGCAGTCAGCCTGCCCTCATGAAGATA AAGCACGTGAGCAGCTTCGTCCGGAAGTATTCTGACACGATAGCCGAGTTACGGGAGCTCCAGCCGTCGG TGAGGGACTTCGAAGTGCGAAGTCTTGTGGGCTGCGGTCACTTCGCCGAAGTGCAGGTGGTTAGAGAGAA GGCCACCGGGGATGTCTACGCCATGAAGATCATGAAGAAGGCGGCTTTGCGGGCCCAGGAGCAGGTTTCA TTTTTCGAGGAGGAGAGGAACATATTATCCCAGAGCACGAGCCCTTGGATCCCCCAATTACAGTACGCCT TTCAGGACAAAAATAACCTTTACCTGGTCATGGAGTATCAGCCTGGAGGGGATTTGCTGTCTCTTCTGAA TAGATATGAGGACCAGTTAGATGAAAACATGATTCAGTTTTACCTTGCTGAGCTGATCTTGGCTGTCCAC AGCGTGCACCAGATGGGATATGTGCATCGAGACATCAAGCCGGAGAACATCCTCATTGACCGAACGGGAC ACATCAAGCTGGTGGATTTTGGATCAGCCGCTAAGATGAATTCAAATAAGGTGGATGCCAAACTCCCTAT TGGGACCCCAGATTACATGGCTCCGGAAGTGTTGACTGTGATGAACGAGGACCGAAGGGGCACCTACGGC CTGGACTGTGACTGGTGGTCTGTCGGAGTGGTTGCCTATGAGATGCTTTACGGGAAGACCCCATTCACGG AGGGAACCTCCGCCCGGACCTTCAACAACATCATGAACTTCCAGCGGTTTTTGAAGTTCCCGGATGACCC CAAAGTTAGCAGTGAGCTCCTTGATCTGATTCAGAGTTTGCTGTGTGTCCAGAAAGAGAGACTGAAGTTC GAGGGTCTCTGTTGCCACCCTTTCTTTGCCAGAACCGACTGGAATAACATCCGGAACTCTCCTCCCCCCT TCGTTCCCACCCTCAAGTCTGACGATGACACCTCCAATTTTGATGAACCAGAGAAGAATTCGTGGGTTTC ATCCTCTCCGTGCCAGCTGAGCCCTTCGGGTTTCTCGGGCGAAGAGCTGCCCTTTGTGGGGTTTTCATAC AGCAAGGCACTGGGGTATCTTGGTAGATCTGAGTCTGTTGTGTCGGGTCTGGACTCCCCTGCCAAGATTA GCTCCATGGAAAAGAAACTTCTCATCAAAAGCAAAGAGCTGCAAGACTCTCAGGACAAGTGTCACAAGAT GGAGCAGGAAATGGCCCGGTTGCATCGCAGAGTGTCAGAGGTGGAGGCTGTGCTTAGTCAGAAGGAGGTG GAGCTGAAGGCCTCTGAGACTCAGAGATCCCTCCTGGAGCAGGACCTTGCCACCTACATCACAGAATGCA GTAGCTTAAAGCGAAGTCTGGAGCAAGCGCGGATGGAGGTGTCTCAGGAGGATGACAAAGCTCTTCAGCT TCTCCACGACATCAGAGAGCAGAGCCGGAAGCTCCAGGAGATCAAGGAGCAGGAGTACCAGGCTCAGGTG GAGGAGATGAGGCTGATGATGAATCAGCTGGAAGAGGACCTCGTGTCAGCCCGAAGACGCAGCGATCTCT ACGAGTCTGAGCTGAGGGAGTCTCGGCTCGCCGCCGAGGAATTCAAGCGGAAGGCAAACGAATGTCAGCA TAAACTGATGAAGGCTAAGGACCTAGGGAAGCCTGAAGTGGGAGAATGTTCCAGACTGGAGAAGATCAAT GCTGAGCAGCAGCTGAAGATTCAGGAGCTCCAGGAGAAGCTGGAGAAGGCTGTAAAAGCCAGCACCGAGG CCACAGAGCTCCTGCAGAACATCCGGCAGGCAAAGGAGCGAGCTGAGAGGGAGCTGGAGAAGCTGCACAA CAGGGAAGATTCTTCCGAAGGCATCAAAAAGAAACTGGTGGAAGCTGAGGAACGCCGCCACTCCCTGGAG AACAAGGTAAAGAGACTAGAGACCATGGAGCGTAGAGAGAACAGACTGAAGGATGACATCCAGACAAAGT CCGAACAGATCCAGCAGATGGCTGATAAAATTCTGGAGCTGGAGGAGAAGCATCGGGAGGCTCAGGTCTC AGCTCAACACCTAGAAGTACACCTGAAGCAGAAGGAACAGCACTATGAGGAAAAGATCAAAGTGTTGGAC AATCAGATAAAGAAGGACTTGGCCGACAAGGAGAGCCTAGAGACCATGATGCAGAGGCACGAAGAGGAGG CCCACGAGAAGGGCAAGATCCTCAGCGAGCAGAAGGCGATGATCAACGCAATGGATTCCAAGATCAGATC CCTGGAGCAGAGGATTGTGGAGCTGTCAGAAGCCAACAAGCTTGCGGCAAACAGCAGTCTCTTCACCCAG AGGAACATGAAGGCCCAGGAAGAGATGATCTCAGAACTCAGGCAGCAGAAATTTTACCTGGAGACACAGG CCGGGAAGCTGGAGGCCCAGAACCGAAAACTGGAAGAGCAACTAGAGAAGATCAGCCACCAAGATCACAG TGACAAGAATCGGCTGCTGGAGCTGGAGACAAGGTTGAGAGAGGTCAGCCTGGAGCATGAGGAGCAGAAG CTGGAGCTGAAGCGGCAGCTCACGGAGCTGCAGCTGTCCCTGCAGGAGCGTGAGTCCCAGCTGACGGCCC TGCAGGCCGCCCGGGCTGCCCTTGAGAGCCAGCTCCGCCAGGCGAAGACGGAGCTGGAGGAGACAACTGC AGAAGCGGAGGAGGAGATCCAGGCACTCACGGCACATCGAGATGAAATCCAGCGCAAATTCGATGCCCTT CGCAACAGCTGTACTGTCATCACCGACCTGGAGGAGCAGCTGAACCAGCTCACCGAGGACAACGCCGAGC TCAACAACCAAAACTTCTACCTGTCCAAACAACTCGATGAGGCTTCGGGTGCCAACGATGAGATTGTGCA GCTGCGAAGCGAGGTGGACCATCTCCGCCGTGAGATCACGGAGCGGGAGATGCAGCTCACCAGCCAGAAG CAAACAATGGAGGCTCTGAAGACGACCTGCACCATGCTGGAGGAGCAGGTCATGGACCTGGAGGCCCTGA ACGACGAGCTGCTGGAGAAGGAGCGCCAGTGGGAGGCCTGGAGGAGCGTCCTCGGGGACGAGAAGTCTCA GTTTGAGTGTCGAGTTCGAGAGCTACAGAGGATGCTGGACACCGAGAAGCAGAGCAGGGCTAGAGCTGAT CAGCGGATCACGGAGTCTCGCCAGGTGGTGGAGCTGGCAGTGAAGGAGCACAAAGCCGAGATTCTCGCGC TGCAGCAGGCTCTCAAAGAGCAGAAGCTGAAAGCTGAGAGCCTGTCGGACAAGCTCAACGACCTGGAGAA GAAGCACGCCATGCTTGAGATGAATGCCCGGAGCTTACAGCAGAAACTGGAGACAGAGCGGGAGCTCAAA CAGAGGCTTCTGGAGGAGCAAGCCAAGTTGCAGCAGCAGATGGACCTGCAGAAGAACCACATCTTCAGAC TGACACAAGGGCTGCAGGAGGCGCTGGACCGGGCGGATCTGCTGAAGACCGAAAGGAGCGACCTGGAATA TCAGCTGGAAAACATTCAGGTTCTCTACTCACACGAGAAGGTGAAAATGGAAGGCACTATTTCTCAACAA ACCAAACTCATTGATTTCCTGCAAGCCAAAATGGACCAGCCCGCTAAAAAGAAAAAGGTTCCTCTGCAGT ACAATGAGCTGAAGCTCGCCCTGGAAAAGGAGAAAGCGCGCTGTGCGGAGCTGGAGGAAGCCCTTCAGAA GACCCGCATCGAACTCCGGTCCGCACGGGAGGAAGCTGCTCACCGCAAAGCCACGGACCACCCGCACCCA TCTACGCCAGCCACTGCGAGGCAGCAGATCGCCATGTCCGCCATCGTGCGGTCGCCTGAGCACCAGCCCA GTGCCATGAGCCTGCTCGCCCCACCATCCAGCCGCAGAAAGGAGGCGTCAACTCCAGAGGAGTTCAGCCG GCGTCTTAAAGAACGTATGCACCACAACATTCCTCACCGGTTTAACGTGGGCCTGAATATGCGAGCCACC AAGTGCGCCGTGTGTCTGGATACTGTGCACTTTGGACGCCAGGCATCCAAATGTCTCGAATGTCAGGTGA TGTGCCATCCCAAGTGTTCCACCTGCTTGCCCGCCACCTGTGGCCTACCTGCCGAATATGCCACACACTT CACCGAGGCCTTCTGCCGAGACAAAGTGAGCTCCCCGGGGCTCCAGAGCAAGGAGCCCAGCAGCAGCTTG CACCTGGAAGGGTGGATGAAAGTGCCCAGGAATAACAAACGAGGACAGCAAGGCTGGGACAGAAAGTACA TTGTCTTAGAGGGTTCAAAAGTCCTCATCTATGACAACGAAGCCAGAGAAGCTGGACAGAGGCCGGTGGA AGAATTTGAGCTGTGCCTTCCCGATGGGGATGTATCTATTCATGGTGCCGTTGGTGCTTCTGAACTTGCA AATACAGCCAAAGCAGATGTCCCATACATACTGAAGATGGAGTCCCATCCGCATACCACCTGCTGGCCTG GGAGAACCCTCTACCTGCTGGCACCCAGCTTTCCTGACAAGCAGCGCTGGGTCACAGCCCTAGAGTCTGT CGTCGCAGGTGGGAGAGTTTCTAGGGAAAAGGCAGAGGCCGATGCTAAATTACTTGGAAACTCTCTGCTG AAGCTGGAAGGTGACGACCGGCTTGACATGAACTGTACCCTGCCCTTCAGCGACCAGGTGGTGCTGGTGG GCACCGAGGAGGGCCTCTATGCGCTGAACGTCTTGAAAAACTCCTTAACCCACATCCCAGGAATCGGAGC AGTCTTCCAGATTTACATCATCAAGGACCTGGAGAAGCTGCTCATGATAGCAGGGGAAGAGCGGGCTCTG TGTTTGGTGGACGTGAAGAAGGTGAAGCAGTCGTTGGCACAGTCACACCTTCCTGCCCAGCCTGACGTCT CCCCCAACATATTTGAAGCCGTCAAAGGCTGCCACCTGTTTGCTGCTGGCAAGATCGAGAACAGCCTCTG CATCTGTGCCGCCATGCCGAGCAAAGTCGTCATCCTGCGCTACAACGACAACCTCAGCAAGTTCTGCATC CGCAAGGAGATTGAGACCTCTGAGCCCTGCAGCTGTATCCACTTCACCAATTACAGTATCCTCATCGGAA CCAACAAGTTCTATGAGATCGACATGAAACAGTACACGCTTGAGGAGTTCCTGGACAAGAATGACCATTC CTTGGCACCTGCTGTGTTCGCATCCTCGACCAACAGCTTCCCCGTCTCCATCGTGCAGGCAAACAGCACC GGGCAGCGGGAAGAGTACCTGCTGTGTTTCCACGAATTTGGGGTGTTCGTGGATTCTTATGGAAGACGTA GCCGCACAGACGATCTCAAGTGGAGTCGCTTACCTCTGGCCTTTGCCTACAGAGAGCCTTATCTGTTTGT GACTCACTTTAACTCCCTGGAAGTAATTGAGATCCAGGCACGTTCCTCACTGGGGACCCCTGCCCGAGCC TATCTGGAAATCCCAAACCCTCGCTACCTGGGCCCCGCAATTTCCTCCGGAGCAATTTACCTGGCCTCCT CATACCAGGACAAGTTAAGGGTCATATGCTGCAAAGGAAACCTCGTGAAGGAGTCTGGCACTGAGCAGCA CCGCGTGCCCTCCACATCCCGCAGCAGCCCTAACAAGCGAGGCCCACCAACATACAACGAGCACATCACC AAACGTGTGGCCTCCAGCCCAGCACCACCAGAAGGCCCCAGCCATCCCCGAGAGCCAAGCACACCCCACC GCTACCGAGACAGAGAGGGCCGGACAGAGCTGCGCAGGGACAAGTCTCCAGGCCGTCCTCTGGAGCGGGA GAAGTCGCCAGGCCGGATGCTCAGCACGAGGAGAGAGCGGTCCCCGGGGAGACTGTTTGAAGACAGCAGC AGGGGCCGGCTGCCTGCGGGAGCCGTGAGGACCCCACTGTCCCAGGTTAACAAGGTCTGGGACCAGTCTT CAGTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001029911
Insert Size	6168 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001029911.1, NP_001025082.1
RefSeq Size	6291 bp
RefSeq ORF	6168 bp
Locus ID	83620
UniProt ID	E9PSL7
Gene Summary	a serine/threonine kinase; involved in regulating G2/M transition [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.